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The map label for this gene is pth

Identifier: 182680480

GI number: 182680480

Start: 4060950

End: 4061546

Strand: Reverse

Name: pth

Synonym: Bind_3581

Alternate gene names: 182680480

Gene position: 4061546-4060950 (Counterclockwise)

Preceding gene: 182680481

Following gene: 182680479

Centisome position: 97.4

GC content: 53.27

Gene sequence:

>597_bases
ATGCTTCTTTTCGTCGGCCTTGGCAATCCCGGCAAGGCCTATAGCGCCAACCGGCACAATATCGGCTTCATGGCCATTGA
TGCCATAGCTCGGGAATATGGAGCGCCGGCTTTTCGTGCGCGTTTCCAAGGTCTCACCAGCGAAATCACTCTCTCTGGCG
AAAAAATCGTCTTGCTGAAGCCAGAGACCTATATGAACGAATCCGGACGCTCGGTCAGCGACGCTGTGCATTTCTATAAG
ATCGACGCGAGCAGGGTCATCGTCTTTCACGATGAGCTCGATCTCGCGCCCGGCAAGGTCCGAGTCAAAAAAGGCGGCGG
GAATGCTGGCCATAATGGGCTGAAATCCATCACGCAGCATATCACCAATGATTACCGGCGCGTGCGCATCGGCATTGGAC
ATCCGGGCATCAAGGACATGGTTCGCCATTATGTCCTTGGCGATTTCTCCAAAACTGAATCGGCCTGGGTCGAAACCCTT
TGCCTTTCAATCGCGGTGAATGCCCCATTGCTTGCCAAGGGCGAGGATGAAAATTTTCAGAATAAAATTTTCCATGCGAT
GGAAACTGCGGGCCTCGCCAAGGATGGCTTGTTCTGA

Upstream 100 bases:

>100_bases
TTCCTCGTGGATTCGCGGGCCTGTTGCGTATCCTCTCGTTCCTTCACCTGCATCGCCCGATGAGTTTTCATTGGAACATG
CTTTTATCGGGCAGGCCGCC

Downstream 100 bases:

>100_bases
CCGGATCCGTGAGTCATGTTTTAAGCCTTTATAAAAACATTAACTTATCGGAAATAGCATCCGCTTTCGGTTGACCCGAT
GGTCTTGATCGGCCGCTTTC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MLLFVGLGNPGKAYSANRHNIGFMAIDAIAREYGAPAFRARFQGLTSEITLSGEKIVLLKPETYMNESGRSVSDAVHFYK
IDASRVIVFHDELDLAPGKVRVKKGGGNAGHNGLKSITQHITNDYRRVRIGIGHPGIKDMVRHYVLGDFSKTESAWVETL
CLSIAVNAPLLAKGEDENFQNKIFHAMETAGLAKDGLF

Sequences:

>Translated_198_residues
MLLFVGLGNPGKAYSANRHNIGFMAIDAIAREYGAPAFRARFQGLTSEITLSGEKIVLLKPETYMNESGRSVSDAVHFYK
IDASRVIVFHDELDLAPGKVRVKKGGGNAGHNGLKSITQHITNDYRRVRIGIGHPGIKDMVRHYVLGDFSKTESAWVETL
CLSIAVNAPLLAKGEDENFQNKIFHAMETAGLAKDGLF
>Mature_198_residues
MLLFVGLGNPGKAYSANRHNIGFMAIDAIAREYGAPAFRARFQGLTSEITLSGEKIVLLKPETYMNESGRSVSDAVHFYK
IDASRVIVFHDELDLAPGKVRVKKGGGNAGHNGLKSITQHITNDYRRVRIGIGHPGIKDMVRHYVLGDFSKTESAWVETL
CLSIAVNAPLLAKGEDENFQNKIFHAMETAGLAKDGLF

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=162, Percent_Identity=34.5679012345679, Blast_Score=100, Evalue=8e-22,
Organism=Escherichia coli, GI1787455, Length=159, Percent_Identity=45.9119496855346, Blast_Score=120, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321983, Length=170, Percent_Identity=30.5882352941176, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_BEII9 (B2IG63)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001834626.1
- GeneID:   6200957
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_3581
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21687; Mature: 21687

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLFVGLGNPGKAYSANRHNIGFMAIDAIAREYGAPAFRARFQGLTSEITLSGEKIVLLK
CEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCEEEEEC
PETYMNESGRSVSDAVHFYKIDASRVIVFHDELDLAPGKVRVKKGGGNAGHNGLKSITQH
CHHHCCCCCCCHHHCEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHH
ITNDYRRVRIGIGHPGIKDMVRHYVLGDFSKTESAWVETLCLSIAVNAPLLAKGEDENFQ
HHCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHH
NKIFHAMETAGLAKDGLF
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLLFVGLGNPGKAYSANRHNIGFMAIDAIAREYGAPAFRARFQGLTSEITLSGEKIVLLK
CEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCEEEEEC
PETYMNESGRSVSDAVHFYKIDASRVIVFHDELDLAPGKVRVKKGGGNAGHNGLKSITQH
CHHHCCCCCCCHHHCEEEEEECCCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHH
ITNDYRRVRIGIGHPGIKDMVRHYVLGDFSKTESAWVETLCLSIAVNAPLLAKGEDENFQ
HHCCEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCHH
NKIFHAMETAGLAKDGLF
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA