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The map label for this gene is alkR [H]

Identifier: 182680428

GI number: 182680428

Start: 4007298

End: 4008236

Strand: Reverse

Name: alkR [H]

Synonym: Bind_3528

Alternate gene names: 182680428

Gene position: 4008236-4007298 (Counterclockwise)

Preceding gene: 182680430

Following gene: 182680427

Centisome position: 96.12

GC content: 61.77

Gene sequence:

>939_bases
ATGCTCGCCTCTCGAGTCGATTTGTTGTCCCAAATGCTCGACCTCGTACGGCTACACGGGGAATTGGTGTTTTCCGCCGA
GTTGAGCCACCCATGGGCGCTGCGGTTCGAGCCGGGGTCGGCCTATTTCTTCGTGGTCCTGGAAGGAAGCTTGATCGCGC
AGGCGGCCGACGCTCCCCCCGTGAAGGCGGTCGCAGGCGACCTCGTCATGCTGCCGCGAGGAACCGGCCATATCCTCGGC
GATGGCAGCGATGCGACTGCGGCAGACGCCGCCGACTTGATGAGAGAGCAGTTCACGGCAGAACAGCTGGGTCTTCGCCA
TGGCGGCAATGGCGAACAGACCAGGGTTATCGCCGGGGCATTCCATTTCGAGAGCACCGCCGTGCCATGGGTCGTCTCAG
CCCTTCCGGCCGTGATCCATATTGCAAAGTCGGGCGGCCAGACTGGCGGATGGCTCGAGGGATTGGCCTATTTCATGATG
ATGGAAGCCCAGGTGGTGCACCCCGGCTCTTCGGTCATGATCTCGCGCCTCATCGACGTTCTGATCATCCGCGTCATCCG
AACCTGGGCGCAGACCAAGAACGCCAGCGACACAGGATGGCTGGGCGCGTTGGGGGACCCCCGCATCAGCCGCGCCCTCA
AGGCCATTCACGACGAACCTTTCCGCAAATGGAGCGTGGCCGACCTGGCAAACGCGGCCGGAATGTCCAGGTCCAGCTTT
GCCGAAAGGTTCTCTTCCCTGGTCAAGGAAGCGCCGTTGTCCTACCAGAATCGATGGCGGCTCACCCTTGCTCACGGGCT
TCTCAGCCAGGCCAATGCCCGTGTCGGCGATGTCGCCAGGCAGGTGGGTTACGACTCCGATGCGGCTTTCAGCCGAGCGT
TCAAAGCTCAATTCGGGATTCCTCCCGCCGGTATTAAATCAGCAGTTTCACAGTCATAG

Upstream 100 bases:

>100_bases
ATATTTCGTCTCTGAAGACCTCTTGAACTTACGGTTTGGCGACATGGAACACCATGATCGGAACCCCGCAAGACATGCTC
CATCGTCCGGATTTGGGGCC

Downstream 100 bases:

>100_bases
TGCCGATATTGACCAATCGGAGGTTCTCCGCGCCGAATGGCGGCTTTGAGAAATCGCGCGGCGGCTGTCCCAGATCGGAG
GGATGGGAGCGGACAGTCCG

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MLASRVDLLSQMLDLVRLHGELVFSAELSHPWALRFEPGSAYFFVVLEGSLIAQAADAPPVKAVAGDLVMLPRGTGHILG
DGSDATAADAADLMREQFTAEQLGLRHGGNGEQTRVIAGAFHFESTAVPWVVSALPAVIHIAKSGGQTGGWLEGLAYFMM
MEAQVVHPGSSVMISRLIDVLIIRVIRTWAQTKNASDTGWLGALGDPRISRALKAIHDEPFRKWSVADLANAAGMSRSSF
AERFSSLVKEAPLSYQNRWRLTLAHGLLSQANARVGDVARQVGYDSDAAFSRAFKAQFGIPPAGIKSAVSQS

Sequences:

>Translated_312_residues
MLASRVDLLSQMLDLVRLHGELVFSAELSHPWALRFEPGSAYFFVVLEGSLIAQAADAPPVKAVAGDLVMLPRGTGHILG
DGSDATAADAADLMREQFTAEQLGLRHGGNGEQTRVIAGAFHFESTAVPWVVSALPAVIHIAKSGGQTGGWLEGLAYFMM
MEAQVVHPGSSVMISRLIDVLIIRVIRTWAQTKNASDTGWLGALGDPRISRALKAIHDEPFRKWSVADLANAAGMSRSSF
AERFSSLVKEAPLSYQNRWRLTLAHGLLSQANARVGDVARQVGYDSDAAFSRAFKAQFGIPPAGIKSAVSQS
>Mature_312_residues
MLASRVDLLSQMLDLVRLHGELVFSAELSHPWALRFEPGSAYFFVVLEGSLIAQAADAPPVKAVAGDLVMLPRGTGHILG
DGSDATAADAADLMREQFTAEQLGLRHGGNGEQTRVIAGAFHFESTAVPWVVSALPAVIHIAKSGGQTGGWLEGLAYFMM
MEAQVVHPGSSVMISRLIDVLIIRVIRTWAQTKNASDTGWLGALGDPRISRALKAIHDEPFRKWSVADLANAAGMSRSSF
AERFSSLVKEAPLSYQNRWRLTLAHGLLSQANARVGDVARQVGYDSDAAFSRAFKAQFGIPPAGIKSAVSQS

Specific function: This protein activates the expression of the alkane 1- monooxygenase AlkM [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786496, Length=266, Percent_Identity=26.3157894736842, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082197, Length=94, Percent_Identity=38.2978723404255, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 33448; Mature: 33448

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLASRVDLLSQMLDLVRLHGELVFSAELSHPWALRFEPGSAYFFVVLEGSLIAQAADAPP
CCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCEEEEEEEECCHHHHCCCCCC
VKAVAGDLVMLPRGTGHILGDGSDATAADAADLMREQFTAEQLGLRHGGNGEQTRVIAGA
HHHHCCCEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
FHFESTAVPWVVSALPAVIHIAKSGGQTGGWLEGLAYFMMMEAQVVHPGSSVMISRLIDV
EECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LIIRVIRTWAQTKNASDTGWLGALGDPRISRALKAIHDEPFRKWSVADLANAAGMSRSSF
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHCCCCHHHH
AERFSSLVKEAPLSYQNRWRLTLAHGLLSQANARVGDVARQVGYDSDAAFSRAFKAQFGI
HHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
PPAGIKSAVSQS
CCHHHHHHHCCC
>Mature Secondary Structure
MLASRVDLLSQMLDLVRLHGELVFSAELSHPWALRFEPGSAYFFVVLEGSLIAQAADAPP
CCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCEEEEEEEECCHHHHCCCCCC
VKAVAGDLVMLPRGTGHILGDGSDATAADAADLMREQFTAEQLGLRHGGNGEQTRVIAGA
HHHHCCCEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
FHFESTAVPWVVSALPAVIHIAKSGGQTGGWLEGLAYFMMMEAQVVHPGSSVMISRLIDV
EECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LIIRVIRTWAQTKNASDTGWLGALGDPRISRALKAIHDEPFRKWSVADLANAAGMSRSSF
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHCCCCHHHH
AERFSSLVKEAPLSYQNRWRLTLAHGLLSQANARVGDVARQVGYDSDAAFSRAFKAQFGI
HHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
PPAGIKSAVSQS
CCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]