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The map label for this gene is thiE [H]

Identifier: 182680422

GI number: 182680422

Start: 4000841

End: 4001485

Strand: Reverse

Name: thiE [H]

Synonym: Bind_3522

Alternate gene names: 182680422

Gene position: 4001485-4000841 (Counterclockwise)

Preceding gene: 182680426

Following gene: 182680421

Centisome position: 95.96

GC content: 64.5

Gene sequence:

>645_bases
ATGTCCGATGCCGCTCCGCGTCTTTATCTCATCACGCCCTTGCTCGACGAGGCGGAGGCCTTCGCCCCTCGCCTCAAGGC
GGCGCTCGACGCCGGCGATGTCGCCTGCCTCCTGCTGCGGATCGCCGATCAAGCGCGTGGTGACGCCAAGACGATCATTC
GCGCCCTCACGCCTTTGGCGCAGCAAAGGGGTGTTGCCTGTTTGATCGAGGACGACACGCAATTGGCGGCGCGCGCCGAT
ACCGATGGCGTCCATATCGAGGCCGATCCCGAGACGCATAAGGATGTTCTCGACGCGGCGATCGCGGCCATGCAGCCGCG
CCGCATCGTGGGCGTCGGGGGGCTGATGAACCGTGATGCGGCCATGATCGCCGGCGAATCCGGCGCCGATTATCTCATGT
TCGGCTTGCCGGATGTCGAGGAAAAACAGGAAGACCTTCTCGAACGCATCGCCTGGTGGGCTGAAATTTTCCAGGTTCCC
TGCGTCGGCCAGGCGCATCGGCTCGAAGACGTGCCGGCCCTCGTGCAAGCGGGCGCCGATTTCATTGCGCTTGGCCGTAT
CGTCTGGGAGGATCCGCGCGGTCCCGCTGCCGTCATTGCCGAGGCCATGGAAAAATGCACTTCGAGCCCCATGCCCGCCC
GATGA

Upstream 100 bases:

>100_bases
CTTCCAAGTTTGATGCTATAGGAGCATATTTCCGGCCAAAATTCCTGGCAGTCCAGGCTGATAGGTCTGCAAGCCCTTTT
TCAAAGAGTTCTCCTCCGCA

Downstream 100 bases:

>100_bases
TGAACAGGGTCAAAAGCGGCTGCGAGCATTCTTGCAAGAGTCATTGGATGAGAAACAGGGTTTTGCCGGTGAGCCTGGCC
TTCAGTCTTGCCTTGCTCAG

Product: thiamine monophosphate synthase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSDAAPRLYLITPLLDEAEAFAPRLKAALDAGDVACLLLRIADQARGDAKTIIRALTPLAQQRGVACLIEDDTQLAARAD
TDGVHIEADPETHKDVLDAAIAAMQPRRIVGVGGLMNRDAAMIAGESGADYLMFGLPDVEEKQEDLLERIAWWAEIFQVP
CVGQAHRLEDVPALVQAGADFIALGRIVWEDPRGPAAVIAEAMEKCTSSPMPAR

Sequences:

>Translated_214_residues
MSDAAPRLYLITPLLDEAEAFAPRLKAALDAGDVACLLLRIADQARGDAKTIIRALTPLAQQRGVACLIEDDTQLAARAD
TDGVHIEADPETHKDVLDAAIAAMQPRRIVGVGGLMNRDAAMIAGESGADYLMFGLPDVEEKQEDLLERIAWWAEIFQVP
CVGQAHRLEDVPALVQAGADFIALGRIVWEDPRGPAAVIAEAMEKCTSSPMPAR
>Mature_213_residues
SDAAPRLYLITPLLDEAEAFAPRLKAALDAGDVACLLLRIADQARGDAKTIIRALTPLAQQRGVACLIEDDTQLAARADT
DGVHIEADPETHKDVLDAAIAAMQPRRIVGVGGLMNRDAAMIAGESGADYLMFGLPDVEEKQEDLLERIAWWAEIFQVPC
VGQAHRLEDVPALVQAGADFIALGRIVWEDPRGPAAVIAEAMEKCTSSPMPAR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22976; Mature: 22844

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAAPRLYLITPLLDEAEAFAPRLKAALDAGDVACLLLRIADQARGDAKTIIRALTPLA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QQRGVACLIEDDTQLAARADTDGVHIEADPETHKDVLDAAIAAMQPRRIVGVGGLMNRDA
HHCCCEEEEECCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
AMIAGESGADYLMFGLPDVEEKQEDLLERIAWWAEIFQVPCVGQAHRLEDVPALVQAGAD
CEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
FIALGRIVWEDPRGPAAVIAEAMEKCTSSPMPAR
HHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDAAPRLYLITPLLDEAEAFAPRLKAALDAGDVACLLLRIADQARGDAKTIIRALTPLA
CCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QQRGVACLIEDDTQLAARADTDGVHIEADPETHKDVLDAAIAAMQPRRIVGVGGLMNRDA
HHCCCEEEEECCCHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
AMIAGESGADYLMFGLPDVEEKQEDLLERIAWWAEIFQVPCVGQAHRLEDVPALVQAGAD
CEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
FIALGRIVWEDPRGPAAVIAEAMEKCTSSPMPAR
HHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA