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The map label for this gene is irlS [H]

Identifier: 182680346

GI number: 182680346

Start: 3911087

End: 3912553

Strand: Reverse

Name: irlS [H]

Synonym: Bind_3446

Alternate gene names: 182680346

Gene position: 3912553-3911087 (Counterclockwise)

Preceding gene: 182680347

Following gene: 182680343

Centisome position: 93.82

GC content: 58.15

Gene sequence:

>1467_bases
TTGCCCTATGATCTTCGTCAGTGGCCGCGCAAACTTTTTCGGGCCGCGACCTTCCGTCTGGCCATTCTCTTCGCACTCGC
TGTTACCGCTGCGACCTCGATCGTCTTCCTCTTCGTGTATCTGCATGTGGCGGTCTATGACGACAAGCAGGTCAGGGCGC
ATCTGATCGAGGAAACCGCTGATGCGGCGATCGAGCCGATGGATCGCATCCAGGGTGAATTCGAACGCCGCCTCGCCAGC
GATTTGCGGATCGTCGATTTCGCGGGTCTCTACGACAAGGACGGGATGCTGATCGCCGGCAATCTTCCGGTCTTGCCTGC
TGATCTGCCGATCGATGGCGGTGCGCACCAACTTGAATCGCCGTCTTTCCAGGATTCCGATGGCTATGGCGGCGGTCCAG
CCCTCTTCGTCGGCCGCACGCGCGCGGACGGCACCACTATCGTGCTCGGCCGCAGCCTGTATGAAGTTCATGCCTTACGC
CATATCGTGTCGCGGGCGCTCATCGTCGGTGTCGTCCCGACCATTCTCCTCACGCTGATCATCGGTGCCGTCTTCAGCTG
GCGTTCGGCGCAACGGCTGCAATTCATCAATCAGCGCATTACCAGCATCATGCAGGGGCATCTGAACGAGCGGCTGCCGG
TCAAAGACGGTGTCGATGACATCGATCATGTCTCTCGCGCGGTGAACCGGATGCTCGATGAGATCGTCCGCTTGCTTTCA
CAGATCAAAAGCGTCGGCGACAATATTGCCCATGATCTGCGTACGCCCCTCGCTCTCATGTGCGCGCGGCTGGAGCGCGG
GCTCGACAGCGAGAACGAGGAGGATCTGCGCGAGAATGCACAATTGGCCCTGCGCGATCTCGATCGGGCCTTGATGACCA
TCACGACCTTGCTCCGCATGTCGGAAATCGAGAACGGCCCCTTGCGCCAGACTTTTGCTCCGGTCGATCTTCGCGAAGTC
TGCCAGCAGATTTTTGAGCTCTACGAGCCCTTGGCTGAGACCAAATCGATCACGCTTCAGCTCATCGAAAAATCTGCTGC
GCCTATGGTTGGCGATTTTCAGCTGCTCATCGAAATGGTCGCGAATCTCGTCGATAATGCCATCAAATTCACGCCTGTCG
GTGGGACGGTGACCTTCGGCGCCTTTCCAAGCCGGCAAGGGCCGGTTCTCCGGGTGGCCGATAATGGCCCTGGCATTCCG
GTCAATGAGCGAGAGGATATTTTCAAGCGATTCTATCGCCATGACCGGAGCCGGCATGTACCGGGCAATGGTCTCGGCCT
TAGCATGGTCGCCATTATCGCCGATCTACACGGCTACGATTTGACGGTGGGGGACAATCAGCCCGGTGCCATCTTTGATT
TCGCGCCTAAACGTATGGATGCAGCAAGGGTCTATCAGGTTGATCCCTGCGAGAAAGATCATTCCCTCATGGGCAGGACG
ACCATGAGGTCCTTCCAGTCTTCGTGA

Upstream 100 bases:

>100_bases
ATCGGCAAGCTGCGCAAGAAGATCGATCAACCGGGCGAGGTCCAAATGATCGAGAGTGTGCGCGGCGCCGGGTTCCGGCT
CAACAATTAAGGTTTTGGCG

Downstream 100 bases:

>100_bases
AGAGTTTCGCTTGTGAAAGCGGGCGCCGTGGGGCGCCCCGCCTGCTTTGATCGTCAGTCTGTAGCAATCTCAGGCCGTAG
CAGCCTCGGGCATGACGCCG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MPYDLRQWPRKLFRAATFRLAILFALAVTAATSIVFLFVYLHVAVYDDKQVRAHLIEETADAAIEPMDRIQGEFERRLAS
DLRIVDFAGLYDKDGMLIAGNLPVLPADLPIDGGAHQLESPSFQDSDGYGGGPALFVGRTRADGTTIVLGRSLYEVHALR
HIVSRALIVGVVPTILLTLIIGAVFSWRSAQRLQFINQRITSIMQGHLNERLPVKDGVDDIDHVSRAVNRMLDEIVRLLS
QIKSVGDNIAHDLRTPLALMCARLERGLDSENEEDLRENAQLALRDLDRALMTITTLLRMSEIENGPLRQTFAPVDLREV
CQQIFELYEPLAETKSITLQLIEKSAAPMVGDFQLLIEMVANLVDNAIKFTPVGGTVTFGAFPSRQGPVLRVADNGPGIP
VNEREDIFKRFYRHDRSRHVPGNGLGLSMVAIIADLHGYDLTVGDNQPGAIFDFAPKRMDAARVYQVDPCEKDHSLMGRT
TMRSFQSS

Sequences:

>Translated_488_residues
MPYDLRQWPRKLFRAATFRLAILFALAVTAATSIVFLFVYLHVAVYDDKQVRAHLIEETADAAIEPMDRIQGEFERRLAS
DLRIVDFAGLYDKDGMLIAGNLPVLPADLPIDGGAHQLESPSFQDSDGYGGGPALFVGRTRADGTTIVLGRSLYEVHALR
HIVSRALIVGVVPTILLTLIIGAVFSWRSAQRLQFINQRITSIMQGHLNERLPVKDGVDDIDHVSRAVNRMLDEIVRLLS
QIKSVGDNIAHDLRTPLALMCARLERGLDSENEEDLRENAQLALRDLDRALMTITTLLRMSEIENGPLRQTFAPVDLREV
CQQIFELYEPLAETKSITLQLIEKSAAPMVGDFQLLIEMVANLVDNAIKFTPVGGTVTFGAFPSRQGPVLRVADNGPGIP
VNEREDIFKRFYRHDRSRHVPGNGLGLSMVAIIADLHGYDLTVGDNQPGAIFDFAPKRMDAARVYQVDPCEKDHSLMGRT
TMRSFQSS
>Mature_487_residues
PYDLRQWPRKLFRAATFRLAILFALAVTAATSIVFLFVYLHVAVYDDKQVRAHLIEETADAAIEPMDRIQGEFERRLASD
LRIVDFAGLYDKDGMLIAGNLPVLPADLPIDGGAHQLESPSFQDSDGYGGGPALFVGRTRADGTTIVLGRSLYEVHALRH
IVSRALIVGVVPTILLTLIIGAVFSWRSAQRLQFINQRITSIMQGHLNERLPVKDGVDDIDHVSRAVNRMLDEIVRLLSQ
IKSVGDNIAHDLRTPLALMCARLERGLDSENEEDLRENAQLALRDLDRALMTITTLLRMSEIENGPLRQTFAPVDLREVC
QQIFELYEPLAETKSITLQLIEKSAAPMVGDFQLLIEMVANLVDNAIKFTPVGGTVTFGAFPSRQGPVLRVADNGPGIPV
NEREDIFKRFYRHDRSRHVPGNGLGLSMVAIIADLHGYDLTVGDNQPGAIFDFAPKRMDAARVYQVDPCEKDHSLMGRTT
MRSFQSS

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=304, Percent_Identity=22.6973684210526, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788279, Length=218, Percent_Identity=28.8990825688073, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787894, Length=307, Percent_Identity=28.6644951140065, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786912, Length=209, Percent_Identity=31.1004784688995, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790861, Length=307, Percent_Identity=27.0358306188925, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=31.5315315315315, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788393, Length=225, Percent_Identity=25.3333333333333, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789808, Length=243, Percent_Identity=27.9835390946502, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789403, Length=325, Percent_Identity=25.5384615384615, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790346, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789149, Length=217, Percent_Identity=29.9539170506912, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790551, Length=228, Percent_Identity=30.2631578947368, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787374, Length=296, Percent_Identity=23.6486486486486, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=230, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788713, Length=214, Percent_Identity=27.5700934579439, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54293; Mature: 54162

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYDLRQWPRKLFRAATFRLAILFALAVTAATSIVFLFVYLHVAVYDDKQVRAHLIEETA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DAAIEPMDRIQGEFERRLASDLRIVDFAGLYDKDGMLIAGNLPVLPADLPIDGGAHQLES
HHHHCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
PSFQDSDGYGGGPALFVGRTRADGTTIVLGRSLYEVHALRHIVSRALIVGVVPTILLTLI
CCCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAVFSWRSAQRLQFINQRITSIMQGHLNERLPVKDGVDDIDHVSRAVNRMLDEIVRLLS
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QIKSVGDNIAHDLRTPLALMCARLERGLDSENEEDLRENAQLALRDLDRALMTITTLLRM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEIENGPLRQTFAPVDLREVCQQIFELYEPLAETKSITLQLIEKSAAPMVGDFQLLIEMV
HHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ANLVDNAIKFTPVGGTVTFGAFPSRQGPVLRVADNGPGIPVNEREDIFKRFYRHDRSRHV
HHHHHCCEEEECCCCEEEECCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCC
PGNGLGLSMVAIIADLHGYDLTVGDNQPGAIFDFAPKRMDAARVYQVDPCEKDHSLMGRT
CCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCHHHEEECCCCCCHHHHHHHH
TMRSFQSS
HHHHHCCC
>Mature Secondary Structure 
PYDLRQWPRKLFRAATFRLAILFALAVTAATSIVFLFVYLHVAVYDDKQVRAHLIEETA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DAAIEPMDRIQGEFERRLASDLRIVDFAGLYDKDGMLIAGNLPVLPADLPIDGGAHQLES
HHHHCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
PSFQDSDGYGGGPALFVGRTRADGTTIVLGRSLYEVHALRHIVSRALIVGVVPTILLTLI
CCCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAVFSWRSAQRLQFINQRITSIMQGHLNERLPVKDGVDDIDHVSRAVNRMLDEIVRLLS
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QIKSVGDNIAHDLRTPLALMCARLERGLDSENEEDLRENAQLALRDLDRALMTITTLLRM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEIENGPLRQTFAPVDLREVCQQIFELYEPLAETKSITLQLIEKSAAPMVGDFQLLIEMV
HHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ANLVDNAIKFTPVGGTVTFGAFPSRQGPVLRVADNGPGIPVNEREDIFKRFYRHDRSRHV
HHHHHCCEEEECCCCEEEECCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCC
PGNGLGLSMVAIIADLHGYDLTVGDNQPGAIFDFAPKRMDAARVYQVDPCEKDHSLMGRT
CCCCCHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCHHHEEECCCCCCHHHHHHHH
TMRSFQSS
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]