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The map label for this gene is yjeA [H]

Identifier: 182680127

GI number: 182680127

Start: 3668830

End: 3669915

Strand: Reverse

Name: yjeA [H]

Synonym: Bind_3224

Alternate gene names: 182680127

Gene position: 3669915-3668830 (Counterclockwise)

Preceding gene: 182680130

Following gene: 182680126

Centisome position: 88.0

GC content: 59.21

Gene sequence:

>1086_bases
ATGACGAGCGAACATCCGGCAAAAACTCGGGCCCTTTCTCATGCCCCCTGGTGGGATAAAAAGGCTTATCATCTGCGGCG
GCCGAAACTCCTCGCCCGCGCCCGGATGATCGAGGCCACTCGGACATTCTTCAAGGCCCAGGACTTCATCGAGGTCGATA
CGGCGATTTTACAAGTCTCGCCGGGCAATGAGACCCATATCGGCGCTTTTGCCACGGCCTGGCGTCCCTTGGGCGGAAAC
AGCGCGCCTCTTTATCTTCACACCTCGCCGGAATTTGCCGCCAAGAAATTGCTGGCCGCCGGGGAGGAGAGGATTTTCAC
CTTCACCCATGCTTTCCGCAACGATGAGCGCGACCGGCTGCATCATCCCGAATTCATGATGCTCGAATGGTATCGCGCAG
AGGCGCCTTTCGAAGTTCTCATGTTCGATTGTGCGCGGCTCCTGGCCCTGACGGCCCAGGCGGCAGAGGCCAAAATCCTG
TCCTATCGGGGCCGTGTCGCCGATCCTTTCACTGAACCGGAACAACTCACCGTCTGCGAGGCTTTCCAGCGTTACGCTGG
GATTGATCTTGAGGCTTTTTTGCCGGGCGGGCCGGAGACCTTTGAAGCCTTTGCCGCGAAAACACGGGAACTCGGCCTCC
GTGTCGCTCCCGACGATGATTGGTCTGATCTTTTCAGCAAAATCCTGTCTGACCGGATCGAACCGAAGCTCGGGCAGGGC
AGGGCGACCTTTTTGACCGATTATCCGGCTTGCGAGGCGGCACTTGCGCGCTTGCGCGCGGATTCGCGTTTTGCTGAACG
GTTCGAGCTTTATGCCTGTGGCGTCGAACTCGCCAATGGGTTTGGTGAATTGACCGATCCCGTTGAACAGGAACGCCGTT
TTGAAGCTGCCATGGCCGAGCGCTTGCGGATCTATGGCGAGGCCTATCCAATCGACCCGGATTTTCTGGCCGCGCTCGCG
CAAATGCCGGAAGCCTCCGGCATTGCATTCGGCTTCGACCGTCTCGCTATGCTGGCGACGGGAAGCGATGATATTGCCGA
GGTTTTATGGACGCCCGTCGCGGAGTCGCCGGGGGAAGGCGCATGA

Upstream 100 bases:

>100_bases
AGACCTGGAATGCGGCTCCGGAATTTTTGCCTGAGCAAACCAGTTGAAAATTTGCTTAGTCGCTTAGCCGCGAACGGCTT
CCTTTTTCAAGCGAAATAGC

Downstream 100 bases:

>100_bases
CGTTCTCTCCCGAGGAAAAAATGCGGCCAGCTTTGCGCAGCACGACCGATCTCGTGGCGGCGGGCCTGATCCCGCCCGAA
CAGGAACGGGCGCTGGCGGA

Product: lysine--tRNA ligase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTSEHPAKTRALSHAPWWDKKAYHLRRPKLLARARMIEATRTFFKAQDFIEVDTAILQVSPGNETHIGAFATAWRPLGGN
SAPLYLHTSPEFAAKKLLAAGEERIFTFTHAFRNDERDRLHHPEFMMLEWYRAEAPFEVLMFDCARLLALTAQAAEAKIL
SYRGRVADPFTEPEQLTVCEAFQRYAGIDLEAFLPGGPETFEAFAAKTRELGLRVAPDDDWSDLFSKILSDRIEPKLGQG
RATFLTDYPACEAALARLRADSRFAERFELYACGVELANGFGELTDPVEQERRFEAAMAERLRIYGEAYPIDPDFLAALA
QMPEASGIAFGFDRLAMLATGSDDIAEVLWTPVAESPGEGA

Sequences:

>Translated_361_residues
MTSEHPAKTRALSHAPWWDKKAYHLRRPKLLARARMIEATRTFFKAQDFIEVDTAILQVSPGNETHIGAFATAWRPLGGN
SAPLYLHTSPEFAAKKLLAAGEERIFTFTHAFRNDERDRLHHPEFMMLEWYRAEAPFEVLMFDCARLLALTAQAAEAKIL
SYRGRVADPFTEPEQLTVCEAFQRYAGIDLEAFLPGGPETFEAFAAKTRELGLRVAPDDDWSDLFSKILSDRIEPKLGQG
RATFLTDYPACEAALARLRADSRFAERFELYACGVELANGFGELTDPVEQERRFEAAMAERLRIYGEAYPIDPDFLAALA
QMPEASGIAFGFDRLAMLATGSDDIAEVLWTPVAESPGEGA
>Mature_360_residues
TSEHPAKTRALSHAPWWDKKAYHLRRPKLLARARMIEATRTFFKAQDFIEVDTAILQVSPGNETHIGAFATAWRPLGGNS
APLYLHTSPEFAAKKLLAAGEERIFTFTHAFRNDERDRLHHPEFMMLEWYRAEAPFEVLMFDCARLLALTAQAAEAKILS
YRGRVADPFTEPEQLTVCEAFQRYAGIDLEAFLPGGPETFEAFAAKTRELGLRVAPDDDWSDLFSKILSDRIEPKLGQGR
ATFLTDYPACEAALARLRADSRFAERFELYACGVELANGFGELTDPVEQERRFEAAMAERLRIYGEAYPIDPDFLAALAQ
MPEASGIAFGFDRLAMLATGSDDIAEVLWTPVAESPGEGA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=344, Percent_Identity=28.1976744186047, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI5031815, Length=344, Percent_Identity=28.1976744186047, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=36.6153846153846, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1790571, Length=346, Percent_Identity=30.9248554913295, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789256, Length=340, Percent_Identity=31.1764705882353, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994340, Length=355, Percent_Identity=29.8591549295775, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17535925, Length=355, Percent_Identity=29.8591549295775, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17535927, Length=355, Percent_Identity=29.8591549295775, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320242, Length=338, Percent_Identity=29.8816568047337, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24640851, Length=344, Percent_Identity=28.4883720930233, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640849, Length=344, Percent_Identity=28.4883720930233, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 40389; Mature: 40258

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEHPAKTRALSHAPWWDKKAYHLRRPKLLARARMIEATRTFFKAQDFIEVDTAILQVS
CCCCCCCHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEEC
PGNETHIGAFATAWRPLGGNSAPLYLHTSPEFAAKKLLAAGEERIFTFTHAFRNDERDRL
CCCCCCCCHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCHHEEEHHHHHCCCCHHHH
HHPEFMMLEWYRAEAPFEVLMFDCARLLALTAQAAEAKILSYRGRVADPFTEPEQLTVCE
CCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AFQRYAGIDLEAFLPGGPETFEAFAAKTRELGLRVAPDDDWSDLFSKILSDRIEPKLGQG
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCCCCC
RATFLTDYPACEAALARLRADSRFAERFELYACGVELANGFGELTDPVEQERRFEAAMAE
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHH
RLRIYGEAYPIDPDFLAALAQMPEASGIAFGFDRLAMLATGSDDIAEVLWTPVAESPGEG
HHHHHCCCCCCCHHHHHHHHHCCCCCCCEECHHHHHHEECCCHHHHHHHHCCCCCCCCCC
A
C
>Mature Secondary Structure 
TSEHPAKTRALSHAPWWDKKAYHLRRPKLLARARMIEATRTFFKAQDFIEVDTAILQVS
CCCCCCHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEEC
PGNETHIGAFATAWRPLGGNSAPLYLHTSPEFAAKKLLAAGEERIFTFTHAFRNDERDRL
CCCCCCCCHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCHHEEEHHHHHCCCCHHHH
HHPEFMMLEWYRAEAPFEVLMFDCARLLALTAQAAEAKILSYRGRVADPFTEPEQLTVCE
CCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AFQRYAGIDLEAFLPGGPETFEAFAAKTRELGLRVAPDDDWSDLFSKILSDRIEPKLGQG
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCCCCC
RATFLTDYPACEAALARLRADSRFAERFELYACGVELANGFGELTDPVEQERRFEAAMAE
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHH
RLRIYGEAYPIDPDFLAALAQMPEASGIAFGFDRLAMLATGSDDIAEVLWTPVAESPGEG
HHHHHCCCCCCCHHHHHHHHHCCCCCCCEECHHHHHHEECCCHHHHHHHHCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA