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The map label for this gene is pbpG [H]

Identifier: 182679951

GI number: 182679951

Start: 3468592

End: 3470754

Strand: Reverse

Name: pbpG [H]

Synonym: Bind_3046

Alternate gene names: 182679951

Gene position: 3470754-3468592 (Counterclockwise)

Preceding gene: 182679953

Following gene: 182679950

Centisome position: 83.23

GC content: 61.4

Gene sequence:

>2163_bases
ATGACGAAAAGCGAGAAAGACCCCCAGCAGCGGTCGAAAAAGGCCCGGCGTCGCTCGCCCTTATGGCGGATTTTTTCGGC
TTTTTGCGCCCTGGCGATCTGGGCCGGCGTCCTGGGCATCGGTATTATCGGTTATTATACCCTGAAAATGCCGCCGATCG
ATCAGCTTAGTGTGCCCAAACGTCCACCTAATATCGCTATTTTGAGCGAGACGGGGACCTTGATCGCTAATCGGGGCGAC
ACGGGCGGGGCGACGATTCGCCTGGCCGATCTGCCACCCTATTTGCCGCGCGCCTTCGTTGCGATCGAAGATCGTCGTTT
CTATCAACATTCCGGCATTGATCCGCTTGGTATTATACGCGCATTGTTCCGCAATGTCGCGGGCGGCGGGGCGATGCAAG
GCGGCTCTACCTTGACACAGCAACTCGCCAAAAATCTGTTCCTGACCCAGGAAAGGACCATGGCGCGCAAGATCCAGGAG
GTGATCCTGGCCGTCTGGCTCGAGCATAAATACAGCAAGGACCAATTGCTGGAACTTTATCTCAACCGCGTCTATTTCGG
TTCCGGCGCCTATGGCGTCGAGGCGGCGGCGGAGAAATATTTCGGCCATCCGGCGAAGCTTGTCACCCTGCCGGAGGCGG
CCATGCTGGCCGGGCTGATGAAGGCGCCAACCAAGCTCGCCCCAACGCATAATCCGCGCGGCGCGGCGGAGCGGGCCGCG
CAAGTCGTCGCCGCCATGCGCGAGGAAGGGTTCATCACCGAATCGATGGCGCAACTCGCCCTCTTGCATCCGGCGCAGGT
GCAGCGCAGCCAGGGGGCGGGCTCGTTCAATTATGCCGCCGATTATGCGATGGACCTGCTCGACGAGACGATTGGCACGA
TCGATCAGGATATTCTCGTGACGACGAGTATTAGCCTGCCTCTGCAGGCCGCCGCCGAAAAAGCCCTGACGGAGGAGCTT
GACCGCAAAGGCGCGCGCTATGGGGTGGCGCAGGGCGCCATGGTCGCGCTCGCGCCCGATGGCGCGATCAAGGCTTTGGT
TGGTGGCCGCAATTATGCCGAAAGCCAGTTCGATCGAGCAGCTTCCGCGCATCGTCAGCCGGGTTCATCCTTCAAGCCCT
TCGTCTATCTCGCCGCGATGGAAGCAGGTCTGACGCCGGATACTGTCCGCGATGACGCGCCGATCAATATCAAGGGCTGG
CAACCGGAAAATTATTCCCATGCCTATCAGGGGCCTGTCACCCTGACGCGGGCTTTGTCGCAATCTCTCAATACAGTGGC
GGCACGGCTCGGGAATGAGGTCGGTCCCAAGGCGGTGATCCGTTTGGCGCATCGGCTCGGCATTGAATCGGACCTGCAAG
CCAATGCCTCGCTGGCGCTCGGCACATCGGAAGTCACGCCGCTCGAAATCACCAGTGCCTATGTGCCCTTTGCCAATGGC
GGCATTGGCGTGCAGCCGCATATCATTCTCAAGGTCAAGACCGCGAGCGGCCAATTACTCTACCAGCGCAAGGGCAGGAG
CAATGGTCGGGTGATCGATGAGGCCTCCCTGTCTATGATGAACCGAATGATGCAGGAAACCCTGATCAGCGGCACGGCGC
ATGGGACAAGCGTGCCGGGCTGGCAGGCGGCGGGCAAGACCGGCACGAGCCAGGATTGGCGCGATGCCTGGTTCATCGGC
TATACGAGCCATATGATTGCGGGGGTTTGGCTCGGCAATGACGATAATTCGCCGACGCGCAAGGCCTCGGGAGGCACATT
GCCAGTGGAAATCTGGTCCCGTTTCATGAAGATCGCACATCAGAACCAGCCGCCGCAGCCGCTGCCCGGTGGCGTATGGC
AAAGCCCGGCGATGCCACAAGTTCTGGCTCCCAATTCGGCTCCTCCGGGTGAGGCGGGGCTTGGCGCGCCGATTGCGGTT
GGAGCAGCGCCGCCGGTCTCCGAGGCGCAAGACTCCGCGCCGCGCGCTCAAGCGCCGACTGCCTATGGGGCGCCAGCCGC
GTCTTCTTCTTCCTCCTCATCCTCATGGGCTCCCGAGAATGACCTCAATGGAATGCTGCCGCCGGCGGCCATTCCGAACA
CTCAGGGGAATGCAGCCCGAGGAAGAGGGCGCCAGCCGCCGCAAACCGAACGTGGCCTGTTCGAAAGTCTGTTCGGCGGA
TGA

Upstream 100 bases:

>100_bases
CCGTTTTTCCCAAGTTAACTGGCCTTTAATCAGCCATATTGAAATCTGACATCCGATGGTTCGGTGACGGAGCCGAAATC
GAGATCCTTTCGCAGGCCGC

Downstream 100 bases:

>100_bases
TCGTCTCCAGACAGCATCCTCATGCGATTATCGTTATGGGCGTGTCGGGCTCTGGCAAATCGACGCTCGGCACGCTTCTG
GCGCAGGCCCTGCACGTTCC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MTKSEKDPQQRSKKARRRSPLWRIFSAFCALAIWAGVLGIGIIGYYTLKMPPIDQLSVPKRPPNIAILSETGTLIANRGD
TGGATIRLADLPPYLPRAFVAIEDRRFYQHSGIDPLGIIRALFRNVAGGGAMQGGSTLTQQLAKNLFLTQERTMARKIQE
VILAVWLEHKYSKDQLLELYLNRVYFGSGAYGVEAAAEKYFGHPAKLVTLPEAAMLAGLMKAPTKLAPTHNPRGAAERAA
QVVAAMREEGFITESMAQLALLHPAQVQRSQGAGSFNYAADYAMDLLDETIGTIDQDILVTTSISLPLQAAAEKALTEEL
DRKGARYGVAQGAMVALAPDGAIKALVGGRNYAESQFDRAASAHRQPGSSFKPFVYLAAMEAGLTPDTVRDDAPINIKGW
QPENYSHAYQGPVTLTRALSQSLNTVAARLGNEVGPKAVIRLAHRLGIESDLQANASLALGTSEVTPLEITSAYVPFANG
GIGVQPHIILKVKTASGQLLYQRKGRSNGRVIDEASLSMMNRMMQETLISGTAHGTSVPGWQAAGKTGTSQDWRDAWFIG
YTSHMIAGVWLGNDDNSPTRKASGGTLPVEIWSRFMKIAHQNQPPQPLPGGVWQSPAMPQVLAPNSAPPGEAGLGAPIAV
GAAPPVSEAQDSAPRAQAPTAYGAPAASSSSSSSSWAPENDLNGMLPPAAIPNTQGNAARGRGRQPPQTERGLFESLFGG

Sequences:

>Translated_720_residues
MTKSEKDPQQRSKKARRRSPLWRIFSAFCALAIWAGVLGIGIIGYYTLKMPPIDQLSVPKRPPNIAILSETGTLIANRGD
TGGATIRLADLPPYLPRAFVAIEDRRFYQHSGIDPLGIIRALFRNVAGGGAMQGGSTLTQQLAKNLFLTQERTMARKIQE
VILAVWLEHKYSKDQLLELYLNRVYFGSGAYGVEAAAEKYFGHPAKLVTLPEAAMLAGLMKAPTKLAPTHNPRGAAERAA
QVVAAMREEGFITESMAQLALLHPAQVQRSQGAGSFNYAADYAMDLLDETIGTIDQDILVTTSISLPLQAAAEKALTEEL
DRKGARYGVAQGAMVALAPDGAIKALVGGRNYAESQFDRAASAHRQPGSSFKPFVYLAAMEAGLTPDTVRDDAPINIKGW
QPENYSHAYQGPVTLTRALSQSLNTVAARLGNEVGPKAVIRLAHRLGIESDLQANASLALGTSEVTPLEITSAYVPFANG
GIGVQPHIILKVKTASGQLLYQRKGRSNGRVIDEASLSMMNRMMQETLISGTAHGTSVPGWQAAGKTGTSQDWRDAWFIG
YTSHMIAGVWLGNDDNSPTRKASGGTLPVEIWSRFMKIAHQNQPPQPLPGGVWQSPAMPQVLAPNSAPPGEAGLGAPIAV
GAAPPVSEAQDSAPRAQAPTAYGAPAASSSSSSSSWAPENDLNGMLPPAAIPNTQGNAARGRGRQPPQTERGLFESLFGG
>Mature_719_residues
TKSEKDPQQRSKKARRRSPLWRIFSAFCALAIWAGVLGIGIIGYYTLKMPPIDQLSVPKRPPNIAILSETGTLIANRGDT
GGATIRLADLPPYLPRAFVAIEDRRFYQHSGIDPLGIIRALFRNVAGGGAMQGGSTLTQQLAKNLFLTQERTMARKIQEV
ILAVWLEHKYSKDQLLELYLNRVYFGSGAYGVEAAAEKYFGHPAKLVTLPEAAMLAGLMKAPTKLAPTHNPRGAAERAAQ
VVAAMREEGFITESMAQLALLHPAQVQRSQGAGSFNYAADYAMDLLDETIGTIDQDILVTTSISLPLQAAAEKALTEELD
RKGARYGVAQGAMVALAPDGAIKALVGGRNYAESQFDRAASAHRQPGSSFKPFVYLAAMEAGLTPDTVRDDAPINIKGWQ
PENYSHAYQGPVTLTRALSQSLNTVAARLGNEVGPKAVIRLAHRLGIESDLQANASLALGTSEVTPLEITSAYVPFANGG
IGVQPHIILKVKTASGQLLYQRKGRSNGRVIDEASLSMMNRMMQETLISGTAHGTSVPGWQAAGKTGTSQDWRDAWFIGY
TSHMIAGVWLGNDDNSPTRKASGGTLPVEIWSRFMKIAHQNQPPQPLPGGVWQSPAMPQVLAPNSAPPGEAGLGAPIAVG
AAPPVSEAQDSAPRAQAPTAYGAPAASSSSSSSSWAPENDLNGMLPPAAIPNTQGNAARGRGRQPPQTERGLFESLFGG

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=544, Percent_Identity=32.1691176470588, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI87082258, Length=316, Percent_Identity=35.7594936708861, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1788867, Length=571, Percent_Identity=28.3712784588441, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=36.3636363636364, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 76745; Mature: 76614

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSEKDPQQRSKKARRRSPLWRIFSAFCALAIWAGVLGIGIIGYYTLKMPPIDQLSVPK
CCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
RPPNIAILSETGTLIANRGDTGGATIRLADLPPYLPRAFVAIEDRRFYQHSGIDPLGIIR
CCCCEEEEECCCCEEEECCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHCCCCHHHHHH
ALFRNVAGGGAMQGGSTLTQQLAKNLFLTQERTMARKIQEVILAVWLEHKYSKDQLLELY
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LNRVYFGSGAYGVEAAAEKYFGHPAKLVTLPEAAMLAGLMKAPTKLAPTHNPRGAAERAA
HHHHEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
QVVAAMREEGFITESMAQLALLHPAQVQRSQGAGSFNYAADYAMDLLDETIGTIDQDILV
HHHHHHHHCCCHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
TTSISLPLQAAAEKALTEELDRKGARYGVAQGAMVALAPDGAIKALVGGRNYAESQFDRA
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHCCCCHHHHHHHHH
ASAHRQPGSSFKPFVYLAAMEAGLTPDTVRDDAPINIKGWQPENYSHAYQGPVTLTRALS
HHHHCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHH
QSLNTVAARLGNEVGPKAVIRLAHRLGIESDLQANASLALGTSEVTPLEITSAYVPFANG
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEHEEECCCCCC
GIGVQPHIILKVKTASGQLLYQRKGRSNGRVIDEASLSMMNRMMQETLISGTAHGTSVPG
CCCCCCEEEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
WQAAGKTGTSQDWRDAWFIGYTSHMIAGVWLGNDDNSPTRKASGGTLPVEIWSRFMKIAH
CCCCCCCCCCCCCCCEEEEEEHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QNQPPQPLPGGVWQSPAMPQVLAPNSAPPGEAGLGAPIAVGAAPPVSEAQDSAPRAQAPT
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCCCCCC
AYGAPAASSSSSSSSWAPENDLNGMLPPAAIPNTQGNAARGRGRQPPQTERGLFESLFGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TKSEKDPQQRSKKARRRSPLWRIFSAFCALAIWAGVLGIGIIGYYTLKMPPIDQLSVPK
CCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
RPPNIAILSETGTLIANRGDTGGATIRLADLPPYLPRAFVAIEDRRFYQHSGIDPLGIIR
CCCCEEEEECCCCEEEECCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHCCCCHHHHHH
ALFRNVAGGGAMQGGSTLTQQLAKNLFLTQERTMARKIQEVILAVWLEHKYSKDQLLELY
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LNRVYFGSGAYGVEAAAEKYFGHPAKLVTLPEAAMLAGLMKAPTKLAPTHNPRGAAERAA
HHHHEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
QVVAAMREEGFITESMAQLALLHPAQVQRSQGAGSFNYAADYAMDLLDETIGTIDQDILV
HHHHHHHHCCCHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
TTSISLPLQAAAEKALTEELDRKGARYGVAQGAMVALAPDGAIKALVGGRNYAESQFDRA
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHCCCCHHHHHHHHH
ASAHRQPGSSFKPFVYLAAMEAGLTPDTVRDDAPINIKGWQPENYSHAYQGPVTLTRALS
HHHHCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHH
QSLNTVAARLGNEVGPKAVIRLAHRLGIESDLQANASLALGTSEVTPLEITSAYVPFANG
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEHEEECCCCCC
GIGVQPHIILKVKTASGQLLYQRKGRSNGRVIDEASLSMMNRMMQETLISGTAHGTSVPG
CCCCCCEEEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCC
WQAAGKTGTSQDWRDAWFIGYTSHMIAGVWLGNDDNSPTRKASGGTLPVEIWSRFMKIAH
CCCCCCCCCCCCCCCEEEEEEHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QNQPPQPLPGGVWQSPAMPQVLAPNSAPPGEAGLGAPIAVGAAPPVSEAQDSAPRAQAPT
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEECCCCCCCHHHCCCCCCCCCC
AYGAPAASSSSSSSSWAPENDLNGMLPPAAIPNTQGNAARGRGRQPPQTERGLFESLFGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]