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The map label for this gene is fliF [H]

Identifier: 182679604

GI number: 182679604

Start: 3063669

End: 3065312

Strand: Reverse

Name: fliF [H]

Synonym: Bind_2689

Alternate gene names: 182679604

Gene position: 3065312-3063669 (Counterclockwise)

Preceding gene: 182679605

Following gene: 182679603

Centisome position: 73.51

GC content: 55.23

Gene sequence:

>1644_bases
ATGACCGTTCGAGAACAAATCGAGCGTCTCTGGGCGAATTTGCTGAAGCTTGGCACGAAGCGTCTGATCACGCTTGCGGT
CGTCGGCGTTTCTGTTTTCGCAATGACGGGATTAGCCGGATATTTCCTGAGTCGGCCGGCGCTCGAGGTCCTCTATTCTG
GCCTGGACAGGCAGGACGTCAGCCGGATCGCTTCCGCTCTCAAGGAGGCGGATATTCCTTTTGATGTCAATGCTGACGGC
AATAGCGTGCTGGTTCGCTTCGGGGAAACGGCGAGAGCGCGCATGCTCCTAGCGGAAAAAGGTCTGCCCAATAGCCCGAA
CGCTGGCAATGAACTGTTCGACAAGCTCGGATCTTTGGGACTGACGTCGTTCATGCAGGAAGTGACCCGTGTGCGCGCCC
TCGAAGGGGAATTGGCCCGGACGATCCAGCTCATGCATGGCGTGAAAGCGGCGCGCGTTCATATCGTCATGCCGGATGAA
GGCTCTTTTCGCCGGACACGTCAACCTCCCTCAGCTTCGGTCGTCGTACGAATGGAGTCGAACGATGATCCGAGTGCGGC
AAAGGCGATTCGTCATCTTGTCGCATCCGCCGTCCCGGGCATGACGGTTGAAGAGGTCACGGTTCTGAATACGGAGGGTG
TGCTGCTCGCATCTGGAGCCGATACAGTCGATGGCGCTCCCAGCGGCATGATCATGCTCGAAAAGGCGGTCTCTCGGGAA
ATCATTGATAATATCAGAAAGACTCTGACGCCTTATTTGGGTCTACGCAATTTCCAGATCAGCGTCGCGGCTCGTCTCAA
CACCGATAAGCGACAGACGAATGAAACGATCTACAATCCCGATTCGAAGGTCGAACGTTCCATTCACGTCACAAGGCAGA
ATCAGAATTCACAGAACTCCACGGTCCAATCCCCGACATCGGTCGAACGCAATCTTCCTCAGGAAAATGCACGGTCCGAT
GATGGCAAGCAATCGACTGAGGAAAGCCAGAAACGGGATGAACTGACCAATTATGAAATCTCTTCAAAAGTGGTCAATAC
CGTCAGCGGCGGCTATGGAATCGACAATCTGTCCGTGGCGGTCCTTGTCAATCGCGCGAGCTTGATCTCCTCCCTGGGTG
AAAAGCCCACGCCGGACGCGTTAGCCAAGCAGATGAACGAAATTGAGATGTTGGTCGCATCAGCGGCGGGTGCTCATAAG
GAGCGAGGAGACATCGTCAAAATTTCGGCTGTGGACTTTATCGATCCAAGTCATGAACTCGAGCCGCAGCCGCCGCCATC
CATCGGTGAAATGTTCCTGCGTCAGTCCACCAATCTCGTCAACGCGGCCACGATCCTCGTTGTGGCGATCCTCCTCATCT
GGTTCGGCTTGCGTCCGGCAACCAAGGCCATTCTGGCGAATACACGAAGCACCGCGACAGCGATCGATCTGAGAGATGAG
GAAGAATTCCAAGCCTTGGAGGATGATGACGCACCGCCGGCCTTGCCTGTCTGGTCGCCGCCTTCTGAACCCAATCTTAT
CGAGGATTTGACCAGTACGCCAAGACACACACCTCAGAAGCGGCTCGAACAGATCGTCGAGTATGACGAGCAGCAAGCGG
CCGCTGTCTTAAAGCAGTGGATTCACCAAGGGGAAGAAGTATGA

Upstream 100 bases:

>100_bases
GCCGCGCTCTTCAGAGCGCGGCCTTTTTCATGATCATATTCAGGCAAGCTTCACCCTGCATCTTCAGTCAAGTTTTATCC
ATAGAGGATTGAGGGGGCAC

Downstream 100 bases:

>100_bases
ATACGCGGCCCGTCAGTGAATTCCTCCGAGACTTTAACGCGCTTGAAATAAAAAATAATAGTGGCGGCTCCGCTGGCGTG
CTGGAGCATGACGTGAATAT

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MTVREQIERLWANLLKLGTKRLITLAVVGVSVFAMTGLAGYFLSRPALEVLYSGLDRQDVSRIASALKEADIPFDVNADG
NSVLVRFGETARARMLLAEKGLPNSPNAGNELFDKLGSLGLTSFMQEVTRVRALEGELARTIQLMHGVKAARVHIVMPDE
GSFRRTRQPPSASVVVRMESNDDPSAAKAIRHLVASAVPGMTVEEVTVLNTEGVLLASGADTVDGAPSGMIMLEKAVSRE
IIDNIRKTLTPYLGLRNFQISVAARLNTDKRQTNETIYNPDSKVERSIHVTRQNQNSQNSTVQSPTSVERNLPQENARSD
DGKQSTEESQKRDELTNYEISSKVVNTVSGGYGIDNLSVAVLVNRASLISSLGEKPTPDALAKQMNEIEMLVASAAGAHK
ERGDIVKISAVDFIDPSHELEPQPPPSIGEMFLRQSTNLVNAATILVVAILLIWFGLRPATKAILANTRSTATAIDLRDE
EEFQALEDDDAPPALPVWSPPSEPNLIEDLTSTPRHTPQKRLEQIVEYDEQQAAAVLKQWIHQGEEV

Sequences:

>Translated_547_residues
MTVREQIERLWANLLKLGTKRLITLAVVGVSVFAMTGLAGYFLSRPALEVLYSGLDRQDVSRIASALKEADIPFDVNADG
NSVLVRFGETARARMLLAEKGLPNSPNAGNELFDKLGSLGLTSFMQEVTRVRALEGELARTIQLMHGVKAARVHIVMPDE
GSFRRTRQPPSASVVVRMESNDDPSAAKAIRHLVASAVPGMTVEEVTVLNTEGVLLASGADTVDGAPSGMIMLEKAVSRE
IIDNIRKTLTPYLGLRNFQISVAARLNTDKRQTNETIYNPDSKVERSIHVTRQNQNSQNSTVQSPTSVERNLPQENARSD
DGKQSTEESQKRDELTNYEISSKVVNTVSGGYGIDNLSVAVLVNRASLISSLGEKPTPDALAKQMNEIEMLVASAAGAHK
ERGDIVKISAVDFIDPSHELEPQPPPSIGEMFLRQSTNLVNAATILVVAILLIWFGLRPATKAILANTRSTATAIDLRDE
EEFQALEDDDAPPALPVWSPPSEPNLIEDLTSTPRHTPQKRLEQIVEYDEQQAAAVLKQWIHQGEEV
>Mature_546_residues
TVREQIERLWANLLKLGTKRLITLAVVGVSVFAMTGLAGYFLSRPALEVLYSGLDRQDVSRIASALKEADIPFDVNADGN
SVLVRFGETARARMLLAEKGLPNSPNAGNELFDKLGSLGLTSFMQEVTRVRALEGELARTIQLMHGVKAARVHIVMPDEG
SFRRTRQPPSASVVVRMESNDDPSAAKAIRHLVASAVPGMTVEEVTVLNTEGVLLASGADTVDGAPSGMIMLEKAVSREI
IDNIRKTLTPYLGLRNFQISVAARLNTDKRQTNETIYNPDSKVERSIHVTRQNQNSQNSTVQSPTSVERNLPQENARSDD
GKQSTEESQKRDELTNYEISSKVVNTVSGGYGIDNLSVAVLVNRASLISSLGEKPTPDALAKQMNEIEMLVASAAGAHKE
RGDIVKISAVDFIDPSHELEPQPPPSIGEMFLRQSTNLVNAATILVVAILLIWFGLRPATKAILANTRSTATAIDLRDEE
EFQALEDDDAPPALPVWSPPSEPNLIEDLTSTPRHTPQKRLEQIVEYDEQQAAAVLKQWIHQGEEV

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=432, Percent_Identity=30.5555555555556, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 59773; Mature: 59642

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVREQIERLWANLLKLGTKRLITLAVVGVSVFAMTGLAGYFLSRPALEVLYSGLDRQDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHH
SRIASALKEADIPFDVNADGNSVLVRFGETARARMLLAEKGLPNSPNAGNELFDKLGSLG
HHHHHHHHHCCCCEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
LTSFMQEVTRVRALEGELARTIQLMHGVKAARVHIVMPDEGSFRRTRQPPSASVVVRMES
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECC
NDDPSAAKAIRHLVASAVPGMTVEEVTVLNTEGVLLASGADTVDGAPSGMIMLEKAVSRE
CCCHHHHHHHHHHHHHHCCCCCCHHEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHH
IIDNIRKTLTPYLGLRNFQISVAARLNTDKRQTNETIYNPDSKVERSIHVTRQNQNSQNS
HHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCCCCCCCCHHHHHHEEEEECCCCCCCC
TVQSPTSVERNLPQENARSDDGKQSTEESQKRDELTNYEISSKVVNTVSGGYGIDNLSVA
CCCCCHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
VLVNRASLISSLGEKPTPDALAKQMNEIEMLVASAAGAHKERGDIVKISAVDFIDPSHEL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCC
EPQPPPSIGEMFLRQSTNLVNAATILVVAILLIWFGLRPATKAILANTRSTATAIDLRDE
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCH
EEFQALEDDDAPPALPVWSPPSEPNLIEDLTSTPRHTPQKRLEQIVEYDEQQAAAVLKQW
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
IHQGEEV
HHCCCCC
>Mature Secondary Structure 
TVREQIERLWANLLKLGTKRLITLAVVGVSVFAMTGLAGYFLSRPALEVLYSGLDRQDV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHH
SRIASALKEADIPFDVNADGNSVLVRFGETARARMLLAEKGLPNSPNAGNELFDKLGSLG
HHHHHHHHHCCCCEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
LTSFMQEVTRVRALEGELARTIQLMHGVKAARVHIVMPDEGSFRRTRQPPSASVVVRMES
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECC
NDDPSAAKAIRHLVASAVPGMTVEEVTVLNTEGVLLASGADTVDGAPSGMIMLEKAVSRE
CCCHHHHHHHHHHHHHHCCCCCCHHEEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHH
IIDNIRKTLTPYLGLRNFQISVAARLNTDKRQTNETIYNPDSKVERSIHVTRQNQNSQNS
HHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHCCCCCCCCCHHHHHHEEEEECCCCCCCC
TVQSPTSVERNLPQENARSDDGKQSTEESQKRDELTNYEISSKVVNTVSGGYGIDNLSVA
CCCCCHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
VLVNRASLISSLGEKPTPDALAKQMNEIEMLVASAAGAHKERGDIVKISAVDFIDPSHEL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCC
EPQPPPSIGEMFLRQSTNLVNAATILVVAILLIWFGLRPATKAILANTRSTATAIDLRDE
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCH
EEFQALEDDDAPPALPVWSPPSEPNLIEDLTSTPRHTPQKRLEQIVEYDEQQAAAVLKQW
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
IHQGEEV
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11013709 [H]