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The map label for this gene is purB [H]

Identifier: 182679597

GI number: 182679597

Start: 3055619

End: 3056926

Strand: Reverse

Name: purB [H]

Synonym: Bind_2682

Alternate gene names: 182679597

Gene position: 3056926-3055619 (Counterclockwise)

Preceding gene: 182679598

Following gene: 182679596

Centisome position: 73.3

GC content: 56.35

Gene sequence:

>1308_bases
ATGATTCCGCGTTATGCCCGTCCCGAAATGGTAGCCATCTGGGAGCCGCGTACGCGTTTCCGCATTTGGTTCGAGATCGA
AGCCTATGCTTGTGAGGCCATGGCCGAGATCGGGATCATCCCCAAGGAATCCGCCAAGGCGATCTGGGAGAAGGCGAAAG
ATGTCGAATTCGATGTCGAACGGATCGATGAAATTGAAAAAATCACCAAGCACGATGTCATCGCCTTTTTGACGCATCTT
GCCGAATTCATCGGCGCCGACGCGCGTTTCGTGCATCAGGGCATGACATCCTCGGATGTGCTCGATACGTGTCTGGCGGT
GCAGCTCTCCCGTGCGTCGGATCTTCTTCTTGCTGATCTTGATGGTTTGCTGGCCGCGTTGAAGCGGCGTGCCTTCGAGC
ACAAAATGACGCCGACGATCGGCCGCTCGCATGGAATTCATGCCGAACCGGTGACCTTTGGTCTCAAGCTCGCTCAGGCC
TATGCCGAATTTTCTCGGGCGCGGGAACGTCTCGTTTATGCGCGCAAGGAGATTGCGACCTGTGCCATCTCCGGCGCCGT
CGGCACATTCGCCAATATTGATCCGCGTGTGGAAACCTATGTCGCTCAAAAGCTCGGCCTCGCGGTCGAGCCTGTTTCGA
CGCAGGTGATTCCGCGCGATCGCCATGCGATGTTTTTCGCGACACTGGGTGTCATCGCTTCGTCCATGGAAAGACTTGCG
GTCGAGGTCCGGCATTTACAGCGCACCGAAGTGCTCGAGGCCGAGGAATATTTCTCGGAAGGCCAGAAGGGCTCCTCGGC
CATGCCGCATAAGCGTAATCCTGTCTTGAGCGAGAATGTCACGGGCCTTGCCCGGCTTGTGCGCGGCATGGCGGTCCCGG
CCATGGAGAATGTCGCGCTCTGGCATGAGCGGGATATTTCGCATTCTTCGGTCGAACGCATGATCGGCCCCGATGCCACG
ATCACCCTGGATTTTGCCTTGGTGCGGCTGACTGGTCTTATTGATAAATTGTTGATCTATCCCGCTAATATGCAAAAGAA
TCTCGACCGGTTGGGTGGCCTTGTCCATTCACAGCGGGTTCTGCTCGCCTTGACCCAGAAGGGCATCAGCCGGGAGGATG
CCTATGCTTATGTCCAGCGCAATGCCATGCCTGTCTGGCGAGGGGAGGGTGAGTTTCTGCCGCTCCTGAAGGCGGATGCG
GATATAGCAAAAGTGTTGAGCCCGGCCGAACTCGAGGAATTGTTCGATCTCGGCTATCACTTCAAGAATGTCGATGTCAT
CTTCGATCGTGTCTTTGGTGAATCCTGA

Upstream 100 bases:

>100_bases
AGCGAATGCCCTTCGTGTCAGGAAAATGCATTCCATCACCAGGCTAGAGTTTTCCCTTCCCCCGTCGGCCTGTTTCCGGC
CACCGTTAAATGAGCTTACG

Downstream 100 bases:

>100_bases
CGCCGCCACTGCGGCTGCACAATCATCGGTACACAATCATCGGTCAATGAGGTTTCCATGAACGAGAGTCTGATGTCGGA
ACAGGTTCAGCTTGATGCTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYARPEMVAIWEPRTRFRIWFEIEAYACEAMAEIGIIPKESAKAIWEKAKDVEFDVERIDEIEKITKHDVIAFLTHL
AEFIGADARFVHQGMTSSDVLDTCLAVQLSRASDLLLADLDGLLAALKRRAFEHKMTPTIGRSHGIHAEPVTFGLKLAQA
YAEFSRARERLVYARKEIATCAISGAVGTFANIDPRVETYVAQKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSMERLA
VEVRHLQRTEVLEAEEYFSEGQKGSSAMPHKRNPVLSENVTGLARLVRGMAVPAMENVALWHERDISHSSVERMIGPDAT
ITLDFALVRLTGLIDKLLIYPANMQKNLDRLGGLVHSQRVLLALTQKGISREDAYAYVQRNAMPVWRGEGEFLPLLKADA
DIAKVLSPAELEELFDLGYHFKNVDVIFDRVFGES

Sequences:

>Translated_435_residues
MIPRYARPEMVAIWEPRTRFRIWFEIEAYACEAMAEIGIIPKESAKAIWEKAKDVEFDVERIDEIEKITKHDVIAFLTHL
AEFIGADARFVHQGMTSSDVLDTCLAVQLSRASDLLLADLDGLLAALKRRAFEHKMTPTIGRSHGIHAEPVTFGLKLAQA
YAEFSRARERLVYARKEIATCAISGAVGTFANIDPRVETYVAQKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSMERLA
VEVRHLQRTEVLEAEEYFSEGQKGSSAMPHKRNPVLSENVTGLARLVRGMAVPAMENVALWHERDISHSSVERMIGPDAT
ITLDFALVRLTGLIDKLLIYPANMQKNLDRLGGLVHSQRVLLALTQKGISREDAYAYVQRNAMPVWRGEGEFLPLLKADA
DIAKVLSPAELEELFDLGYHFKNVDVIFDRVFGES
>Mature_435_residues
MIPRYARPEMVAIWEPRTRFRIWFEIEAYACEAMAEIGIIPKESAKAIWEKAKDVEFDVERIDEIEKITKHDVIAFLTHL
AEFIGADARFVHQGMTSSDVLDTCLAVQLSRASDLLLADLDGLLAALKRRAFEHKMTPTIGRSHGIHAEPVTFGLKLAQA
YAEFSRARERLVYARKEIATCAISGAVGTFANIDPRVETYVAQKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSMERLA
VEVRHLQRTEVLEAEEYFSEGQKGSSAMPHKRNPVLSENVTGLARLVRGMAVPAMENVALWHERDISHSSVERMIGPDAT
ITLDFALVRLTGLIDKLLIYPANMQKNLDRLGGLVHSQRVLLALTQKGISREDAYAYVQRNAMPVWRGEGEFLPLLKADA
DIAKVLSPAELEELFDLGYHFKNVDVIFDRVFGES

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=432, Percent_Identity=26.3888888888889, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI183227688, Length=415, Percent_Identity=27.4698795180723, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1787376, Length=381, Percent_Identity=27.8215223097113, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=26.010101010101, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=363, Percent_Identity=26.4462809917355, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=390, Percent_Identity=26.6666666666667, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24647570, Length=382, Percent_Identity=25.130890052356, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48712; Mature: 48712

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYARPEMVAIWEPRTRFRIWFEIEAYACEAMAEIGIIPKESAKAIWEKAKDVEFDVE
CCCCCCCCCEEEEECCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHH
RIDEIEKITKHDVIAFLTHLAEFIGADARFVHQGMTSSDVLDTCLAVQLSRASDLLLADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHH
DGLLAALKRRAFEHKMTPTIGRSHGIHAEPVTFGLKLAQAYAEFSRARERLVYARKEIAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVETYVAQKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSMERLA
HHHHCCHHCCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VEVRHLQRTEVLEAEEYFSEGQKGSSAMPHKRNPVLSENVTGLARLVRGMAVPAMENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
WHERDISHSSVERMIGPDATITLDFALVRLTGLIDKLLIYPANMQKNLDRLGGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LLALTQKGISREDAYAYVQRNAMPVWRGEGEFLPLLKADADIAKVLSPAELEELFDLGYH
HHHHHHHCCCHHHHHHHHHHCCCCEECCCCCEEEHHHCCCHHHHHCCHHHHHHHHHHCCC
FKNVDVIFDRVFGES
CCCHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYARPEMVAIWEPRTRFRIWFEIEAYACEAMAEIGIIPKESAKAIWEKAKDVEFDVE
CCCCCCCCCEEEEECCCCEEEEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHH
RIDEIEKITKHDVIAFLTHLAEFIGADARFVHQGMTSSDVLDTCLAVQLSRASDLLLADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHH
DGLLAALKRRAFEHKMTPTIGRSHGIHAEPVTFGLKLAQAYAEFSRARERLVYARKEIAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVETYVAQKLGLAVEPVSTQVIPRDRHAMFFATLGVIASSMERLA
HHHHCCHHCCCCCCCHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VEVRHLQRTEVLEAEEYFSEGQKGSSAMPHKRNPVLSENVTGLARLVRGMAVPAMENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
WHERDISHSSVERMIGPDATITLDFALVRLTGLIDKLLIYPANMQKNLDRLGGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LLALTQKGISREDAYAYVQRNAMPVWRGEGEFLPLLKADADIAKVLSPAELEELFDLGYH
HHHHHHHCCCHHHHHHHHHHCCCCEECCCCCEEEHHHCCCHHHHHCCHHHHHHHHHHCCC
FKNVDVIFDRVFGES
CCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]