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The map label for this gene is pdxJ [H]

Identifier: 182679396

GI number: 182679396

Start: 2785516

End: 2786298

Strand: Reverse

Name: pdxJ [H]

Synonym: Bind_2444

Alternate gene names: 182679396

Gene position: 2786298-2785516 (Counterclockwise)

Preceding gene: 182679397

Following gene: 182679395

Centisome position: 66.82

GC content: 60.92

Gene sequence:

>783_bases
ATGTCTGCTGGGAAAGCGTTGCGTCTTGGAGTGAATATTGATCATGTCGCGACCTTGCGCAATGCGCGGGGTGGAGCGCG
GCCTGACCCCGTGCGCGCCGCCTTGCTGGCGATCGAAGCGGGCGCGGATGGCATTACCGCCCATTTGCGTGAAGACCGGC
GGCATATTCAGGATGGCGACATGATTCGCCTCAAGGCGGCGATCAACAAGCCCTTGAATTTCGAAATGGCGGCGACCGAG
CAGATGCTCGACATCGCTTTGAAGATCAAACCGCATGCGGCTTGCCTGGTGCCGGAGCGGCGGACCGAGCGCACGACCGA
AGGCGGGCTCGATGTGCTGGGCGGTCTTGCCACGCTCAAGCCTTTTGTTGCGGAACTCGGCAGGGCGGGCATTCGTGTAT
CCCTGTTCATCGAACCCTCTTTCGAGGCCATCGACGCCGCCGTGGACATTGGCGCGCCGGTGGTCGAATTGCATACGGGT
GCCTGGTGCCATGCAGTCGAGGAGGGTGAAAGAGAGAAGGCCGCCCGGGAATTCGAGCGGCTGCGTCTTGCGGCACAACG
GGCGGCGAGGGCGGGTCTTGAATGCCATGCCGGCCATGGACTTGATTTCGAGACGGCACGCATTCTCTCCGCCTTGCCGG
AAATCGTTGAACTGAACATCGGCCATTTCTTGATTGGGGAAGCTGTGTTCATTGGCTTGCCCGAGGCGATCAGACAGATG
CGAAAGGCGATGGACGCGGGCCGCGCGACATATGTCGAAAGCCAATCCCTGCGATTGAACTGA

Upstream 100 bases:

>100_bases
TCATTGATGTTGAAGTCAACGACTTGAAGCATCTTAACGCCATCATGCAACAATTGCGTGCGCGGCCCGTGGTGCATCAG
GTGGAAAGGGTGAACGGCTG

Downstream 100 bases:

>100_bases
CGCCTATGATTATCGGCTTCGGCAATGATCTCTGCGATATCCGCCGGATCGAGGAATCACTCGCCCGGTTCGGCGAACGG
TTCATTGCGCGCTGCTTCAC

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSAGKALRLGVNIDHVATLRNARGGARPDPVRAALLAIEAGADGITAHLREDRRHIQDGDMIRLKAAINKPLNFEMAATE
QMLDIALKIKPHAACLVPERRTERTTEGGLDVLGGLATLKPFVAELGRAGIRVSLFIEPSFEAIDAAVDIGAPVVELHTG
AWCHAVEEGEREKAAREFERLRLAAQRAARAGLECHAGHGLDFETARILSALPEIVELNIGHFLIGEAVFIGLPEAIRQM
RKAMDAGRATYVESQSLRLN

Sequences:

>Translated_260_residues
MSAGKALRLGVNIDHVATLRNARGGARPDPVRAALLAIEAGADGITAHLREDRRHIQDGDMIRLKAAINKPLNFEMAATE
QMLDIALKIKPHAACLVPERRTERTTEGGLDVLGGLATLKPFVAELGRAGIRVSLFIEPSFEAIDAAVDIGAPVVELHTG
AWCHAVEEGEREKAAREFERLRLAAQRAARAGLECHAGHGLDFETARILSALPEIVELNIGHFLIGEAVFIGLPEAIRQM
RKAMDAGRATYVESQSLRLN
>Mature_259_residues
SAGKALRLGVNIDHVATLRNARGGARPDPVRAALLAIEAGADGITAHLREDRRHIQDGDMIRLKAAINKPLNFEMAATEQ
MLDIALKIKPHAACLVPERRTERTTEGGLDVLGGLATLKPFVAELGRAGIRVSLFIEPSFEAIDAAVDIGAPVVELHTGA
WCHAVEEGEREKAAREFERLRLAAQRAARAGLECHAGHGLDFETARILSALPEIVELNIGHFLIGEAVFIGLPEAIRQMR
KAMDAGRATYVESQSLRLN

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=238, Percent_Identity=52.9411764705882, Blast_Score=245, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 28120; Mature: 27989

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00449 ATPASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGKALRLGVNIDHVATLRNARGGARPDPVRAALLAIEAGADGITAHLREDRRHIQDGD
CCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHHHHCCCCC
MIRLKAAINKPLNFEMAATEQMLDIALKIKPHAACLVPERRTERTTEGGLDVLGGLATLK
EEEEEEHHCCCCCCCHHHHHHHHHHHEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHHH
PFVAELGRAGIRVSLFIEPSFEAIDAAVDIGAPVVELHTGAWCHAVEEGEREKAAREFER
HHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LRLAAQRAARAGLECHAGHGLDFETARILSALPEIVELNIGHFLIGEAVFIGLPEAIRQM
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHH
RKAMDAGRATYVESQSLRLN
HHHHHCCCEEEEECCCEECC
>Mature Secondary Structure 
SAGKALRLGVNIDHVATLRNARGGARPDPVRAALLAIEAGADGITAHLREDRRHIQDGD
CCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHEEEECCCCCHHHHHHHHHHHCCCCC
MIRLKAAINKPLNFEMAATEQMLDIALKIKPHAACLVPERRTERTTEGGLDVLGGLATLK
EEEEEEHHCCCCCCCHHHHHHHHHHHEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHHH
PFVAELGRAGIRVSLFIEPSFEAIDAAVDIGAPVVELHTGAWCHAVEEGEREKAAREFER
HHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
LRLAAQRAARAGLECHAGHGLDFETARILSALPEIVELNIGHFLIGEAVFIGLPEAIRQM
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCHHHHHHH
RKAMDAGRATYVESQSLRLN
HHHHHCCCEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 9870699 [H]