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The map label for this gene is rbsB [H]

Identifier: 182679296

GI number: 182679296

Start: 2677179

End: 2678099

Strand: Reverse

Name: rbsB [H]

Synonym: Bind_2340

Alternate gene names: 182679296

Gene position: 2678099-2677179 (Counterclockwise)

Preceding gene: 182679297

Following gene: 182679295

Centisome position: 64.22

GC content: 57.33

Gene sequence:

>921_bases
ATGACCATCAAGGCGTTATTTCTGGCAGGAATGACTGCTGTGCTCACGGTCAATGCCGCCTCGGCGAAGGACTTGCGTTC
TATTGGCTTGACTCTTGGCTCTCTCGGCAATCCCTATTTTTTGGCGCTCGTCGATGGTGCGACCAAACAGGCCAAGACGA
TCAATCCCGCGGTCAAGGTGAATGCTGTCTCTTCCGAATATGATCTTGGCAAACAGTCCAGTCAGATCGACAATTTCATT
GCGTCCGGAGTGGATCTCATTCTGGTGGCCGCAGCCGACCCGAAAGCCATTGGATCGGCTGTGCAACGCGCCCAGGCGGC
TGGGGTCACGGTCATCGCGGTTGACGTGGAGGCCGATAAGGTCGACGCGACCGTTCAAACAGACAATAAGCAGGCCGGCG
CGATCTCCTGCGATTATCTGGTGAGCAAGATTGGCGGCAAAGGGAATGTCATCATCGAAAGCGGCCCGCCAACCTCGTCC
GTGCTTCAACGTGTCGAAGGCTGCAAAATGGCTCTGGCGGCCTATCCCGATATCAAGATCTTGTCGGACAATCAGAACGG
CAAGGTTTCGCGGGAGGGAGGAAAGGATGTGATGCTGAGCTATCTCACCCGTTTCCCCAATATCCAGGGCGTGTTCACGA
TCGCGGATCCCCAGGCGATCGGCTCCATCCTGGCGATCAAACAGCTTAGCCGATCCGGGATCCTCGTCACGTCGGTGGAT
GGCTCCCCCGATGCCGTCGAGGCCATGAGGAGCGATACGCCGTTGCAGGCCTCGGCCGCTCAGGACCCAGCCGGGCTGGG
GTGGCGTGCGGTGCAAATCGGCTATGACATCATGAACGGGAAAAAGCCGGAGAACCGGGTAACGCAACTTCCTCCCATTC
TCGTCACACGTGACAATGTGAATGGCTATAAAGGGTGGTAG

Upstream 100 bases:

>100_bases
GACACTTTTAGTTGTCGCATGCTCCTTTCAGGAACGACTGCTGGCTTTGTCTCGGCAGGCGATGGAAGGAGTGCGTCCAT
CGAACACGAAGGAAAGCAAA

Downstream 100 bases:

>100_bases
AAGTCTGCGGATAGACACCAAGTGTTGCCATGAGAGAGACTCGGTCCGTTTTTGAGCTTTCGCCTTTTTCAAAAGCATGG
CGAGAATTTGATGAGAATCC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTIKALFLAGMTAVLTVNAASAKDLRSIGLTLGSLGNPYFLALVDGATKQAKTINPAVKVNAVSSEYDLGKQSSQIDNFI
ASGVDLILVAAADPKAIGSAVQRAQAAGVTVIAVDVEADKVDATVQTDNKQAGAISCDYLVSKIGGKGNVIIESGPPTSS
VLQRVEGCKMALAAYPDIKILSDNQNGKVSREGGKDVMLSYLTRFPNIQGVFTIADPQAIGSILAIKQLSRSGILVTSVD
GSPDAVEAMRSDTPLQASAAQDPAGLGWRAVQIGYDIMNGKKPENRVTQLPPILVTRDNVNGYKGW

Sequences:

>Translated_306_residues
MTIKALFLAGMTAVLTVNAASAKDLRSIGLTLGSLGNPYFLALVDGATKQAKTINPAVKVNAVSSEYDLGKQSSQIDNFI
ASGVDLILVAAADPKAIGSAVQRAQAAGVTVIAVDVEADKVDATVQTDNKQAGAISCDYLVSKIGGKGNVIIESGPPTSS
VLQRVEGCKMALAAYPDIKILSDNQNGKVSREGGKDVMLSYLTRFPNIQGVFTIADPQAIGSILAIKQLSRSGILVTSVD
GSPDAVEAMRSDTPLQASAAQDPAGLGWRAVQIGYDIMNGKKPENRVTQLPPILVTRDNVNGYKGW
>Mature_305_residues
TIKALFLAGMTAVLTVNAASAKDLRSIGLTLGSLGNPYFLALVDGATKQAKTINPAVKVNAVSSEYDLGKQSSQIDNFIA
SGVDLILVAAADPKAIGSAVQRAQAAGVTVIAVDVEADKVDATVQTDNKQAGAISCDYLVSKIGGKGNVIIESGPPTSSV
LQRVEGCKMALAAYPDIKILSDNQNGKVSREGGKDVMLSYLTRFPNIQGVFTIADPQAIGSILAIKQLSRSGILVTSVDG
SPDAVEAMRSDTPLQASAAQDPAGLGWRAVQIGYDIMNGKKPENRVTQLPPILVTRDNVNGYKGW

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=275, Percent_Identity=32, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1790526, Length=287, Percent_Identity=27.1777003484321, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790674, Length=195, Percent_Identity=33.8461538461538, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788473, Length=336, Percent_Identity=26.1904761904762, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 31900; Mature: 31769

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKALFLAGMTAVLTVNAASAKDLRSIGLTLGSLGNPYFLALVDGATKQAKTINPAVKV
CCCHHHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEE
NAVSSEYDLGKQSSQIDNFIASGVDLILVAAADPKAIGSAVQRAQAAGVTVIAVDVEADK
EEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCC
VDATVQTDNKQAGAISCDYLVSKIGGKGNVIIESGPPTSSVLQRVEGCKMALAAYPDIKI
CCEEEEECCCCCCCEEHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCEEEEEECCCEEE
LSDNQNGKVSREGGKDVMLSYLTRFPNIQGVFTIADPQAIGSILAIKQLSRSGILVTSVD
EECCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECC
GSPDAVEAMRSDTPLQASAAQDPAGLGWRAVQIGYDIMNGKKPENRVTQLPPILVTRDNV
CCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEHHHCCCCCCCHHHCCCCEEEEECCC
NGYKGW
CCCCCC
>Mature Secondary Structure 
TIKALFLAGMTAVLTVNAASAKDLRSIGLTLGSLGNPYFLALVDGATKQAKTINPAVKV
CCHHHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEE
NAVSSEYDLGKQSSQIDNFIASGVDLILVAAADPKAIGSAVQRAQAAGVTVIAVDVEADK
EEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCC
VDATVQTDNKQAGAISCDYLVSKIGGKGNVIIESGPPTSSVLQRVEGCKMALAAYPDIKI
CCEEEEECCCCCCCEEHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCEEEEEECCCEEE
LSDNQNGKVSREGGKDVMLSYLTRFPNIQGVFTIADPQAIGSILAIKQLSRSGILVTSVD
EECCCCCEEECCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCEEEEECC
GSPDAVEAMRSDTPLQASAAQDPAGLGWRAVQIGYDIMNGKKPENRVTQLPPILVTRDNV
CCCHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEHHHCCCCCCCHHHCCCCEEEEECCC
NGYKGW
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]