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The map label for this gene is queD [H]

Identifier: 182679141

GI number: 182679141

Start: 2488792

End: 2489145

Strand: Direct

Name: queD [H]

Synonym: Bind_2178

Alternate gene names: 182679141

Gene position: 2488792-2489145 (Clockwise)

Preceding gene: 182679140

Following gene: 182679143

Centisome position: 59.68

GC content: 52.54

Gene sequence:

>354_bases
ATGAAAATCACCCAAGCCTTTCATTTCGAGGCCGCCCATCGTTTGCCAAAAGTGCCGGAAGGCCATCGCTGCGCCAACTT
GCACGGCCATTCCTACCGCGTCGAACTGTATCTCGAAGGACCAGTCGATCCGGATACCGGCTTTGTTATCGACTTTTTCG
ATATTGAAGCCGTTTTCGGCCCGTTGTTGCACAGGCTCGATCATCATTATCTGAATGAGATCGAAGGGCTCGAAAACCCA
ACAGCCGAACTCATCGCTGTCTGGATCTGGGACCGTGTCAAACCGTCACTGCCGCAACTCGTCAAGGTCATCGTCTACGA
GACACCAGATTGTTGGGCTGAATACGAAGGGTGA

Upstream 100 bases:

>100_bases
ACGCCAATCTGGCGCGAAACACCACGGAAGCCACGGCCTATTGCCTTGCGCATCCGCAGTGGCGGCTCAGCCTGCAAACC
CATAAATTGATTGGTATTCC

Downstream 100 bases:

>100_bases
TACAATCATCATCCTCCCTCGATCAGCATTTTTCAGCCGACATACACTCATCAATGCCGCTATCATTGTATCGATAGCTC
CAAAATGACCTGATGGACGA

Product: queuosine biosynthesis protein QueD

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MKITQAFHFEAAHRLPKVPEGHRCANLHGHSYRVELYLEGPVDPDTGFVIDFFDIEAVFGPLLHRLDHHYLNEIEGLENP
TAELIAVWIWDRVKPSLPQLVKVIVYETPDCWAEYEG

Sequences:

>Translated_117_residues
MKITQAFHFEAAHRLPKVPEGHRCANLHGHSYRVELYLEGPVDPDTGFVIDFFDIEAVFGPLLHRLDHHYLNEIEGLENP
TAELIAVWIWDRVKPSLPQLVKVIVYETPDCWAEYEG
>Mature_117_residues
MKITQAFHFEAAHRLPKVPEGHRCANLHGHSYRVELYLEGPVDPDTGFVIDFFDIEAVFGPLLHRLDHHYLNEIEGLENP
TAELIAVWIWDRVKPSLPQLVKVIVYETPDCWAEYEG

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789124, Length=113, Percent_Identity=58.4070796460177, Blast_Score=140, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19550074, Length=132, Percent_Identity=34.0909090909091, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585378, Length=132, Percent_Identity=34.0909090909091, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 13520; Mature: 13520

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITQAFHFEAAHRLPKVPEGHRCANLHGHSYRVELYLEGPVDPDTGFVIDFFDIEAVFG
CCCCHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHH
PLLHRLDHHYLNEIEGLENPTAELIAVWIWDRVKPSLPQLVKVIVYETPDCWAEYEG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKITQAFHFEAAHRLPKVPEGHRCANLHGHSYRVELYLEGPVDPDTGFVIDFFDIEAVFG
CCCCHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCCCEEEEHHHHHHHHH
PLLHRLDHHYLNEIEGLENPTAELIAVWIWDRVKPSLPQLVKVIVYETPDCWAEYEG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]