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The map label for this gene is leuC [H]

Identifier: 182679066

GI number: 182679066

Start: 2404881

End: 2406293

Strand: Reverse

Name: leuC [H]

Synonym: Bind_2103

Alternate gene names: 182679066

Gene position: 2406293-2404881 (Counterclockwise)

Preceding gene: 182679067

Following gene: 182679063

Centisome position: 57.7

GC content: 59.52

Gene sequence:

>1413_bases
ATGGCGCAACCGCGTACCCTCTATGATAAAATCTGGGATGATCATGTGGTCGAGACCCAGGCCGATGGCACGAGCCTTCT
CTATATCGACAGGCACCTCGTCCATGAGGTGACGAGCCCGCAAGCCTTCGAGGGTCTGCGCATGGCTGGCCGCAAGGTTC
GCGCGCCCCAGAAAACCTTGGCTGTCGTTGATCATAATGTGCCGACCACCGATCGCAGCCATGGGATTGAAGATCCCGAA
AGCCGGATCCAGGTTGCCCAGCTTGCCGCCAATGCGGCTGAATTCGGCATTGAATATTTCGATGCGCTGGATCATCGGCA
GGGTGTCGTTCATATCATCGGGCCGGAGCAAGGGTTCACCCTGCCGGGCACGACGATTGTCTGCGGTGATAGCCACACCT
CGACGCATGGAGCCTTTGGCGCCTTGGCGCATGGCATTGGTACGTCCGAGGTCGAGCATGTGCTGGCGACACAGACATTG
ATTCAGGCCAAGGCGAAGAACATGCGCGTTCTCGTCGATGGCGTTCTGCCCGATCACGTCAGCGCCAAGGATCTCATCCT
TGCGATCATCGGCCAGATCGGCACGGCGGGCGGTACTGGCCATGTCATCGAATATGCTGGCGAGGCGATCCGCGCCCTTT
CGGTGGAAGGCCGTATGACGGTCTGCAACATGACGATTGAGGGCGGCGCGCGTGCCGGCATGATCGCGCCGGACGCCAAA
ACCTTTGCCTATCTGAAGGATCGTCCCAAATCGCCGCGCGGCGCCGCCTGGGACGCGGCCGTGGCCTATTGGGAAACCCT
GCATTCCGATGAAGGTGCGGTTTTCGATCGGGAAATTCGTCTCGACGCAGCCAATTTGCCGCCGACCGTGACTTGGGGCA
CGAGCCCTGAGGATGTCGCGTCCATCAATGGCGTGGTTCCCTCGCCTCAGGACGCAACGAGCGAGGCGAAGAAAGCCTCG
ATTGCCCGTGCCCTCGAATATATGGGCTTGAAGGGTGGCGAGAAACTGACCTCGATCGCCATTGATCGCGTGTTTATCGG
CTCCTGTACCAATGGCCGGATCGAGGATTTGCGGGAAGCCGCGAAAATCGTCCAAGGCAAGCATATTGCTGCTTCGGTCA
ACGGTCTCGTCGTGCCGGGGTCTGGCTTGGTCAAGGAACAAGCGGAAGCTGAAGGTCTCGATGTGATCTTCAAAGCGGCG
GGCTTTGAATGGCGCGAACCTGGATGCTCGATGTGCCTTGCCATGAACCCCGACAAGCTTGCGCCTGGCGAACGCTGCGC
CTCCACGTCCAACCGTAATTTCGAGGGACGTCAAGGCTTCAAGGGACGCACACATCTCGTCTCTCCGGCCATGGCGGCGG
CAGCCGGTATTGCCGGCCATTTTCGTCGACATTCGCGATTGGCACTAAGATAA

Upstream 100 bases:

>100_bases
ATGAATTCGATGCCCGCCGGAACACCAAGCGTGCAGGATAGGCTCGACTTCGCTACCAGCCCTAACGATTCCATAAATGA
AGAGGACCGACAGGACAGCC

Downstream 100 bases:

>100_bases
AGGGAGCCATCCCGCGTCGATCGAAGCGGGATGGCTCCAGCTTCTCATGAACTCTGCCTCGCATTAGCTCTTGGCGATAT
TGACCTTGCCGAGCGCCTCT

Product: 3-isopropylmalate dehydratase, large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAQPRTLYDKIWDDHVVETQADGTSLLYIDRHLVHEVTSPQAFEGLRMAGRKVRAPQKTLAVVDHNVPTTDRSHGIEDPE
SRIQVAQLAANAAEFGIEYFDALDHRQGVVHIIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQAKAKNMRVLVDGVLPDHVSAKDLILAIIGQIGTAGGTGHVIEYAGEAIRALSVEGRMTVCNMTIEGGARAGMIAPDAK
TFAYLKDRPKSPRGAAWDAAVAYWETLHSDEGAVFDREIRLDAANLPPTVTWGTSPEDVASINGVVPSPQDATSEAKKAS
IARALEYMGLKGGEKLTSIAIDRVFIGSCTNGRIEDLREAAKIVQGKHIAASVNGLVVPGSGLVKEQAEAEGLDVIFKAA
GFEWREPGCSMCLAMNPDKLAPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAGIAGHFRRHSRLALR

Sequences:

>Translated_470_residues
MAQPRTLYDKIWDDHVVETQADGTSLLYIDRHLVHEVTSPQAFEGLRMAGRKVRAPQKTLAVVDHNVPTTDRSHGIEDPE
SRIQVAQLAANAAEFGIEYFDALDHRQGVVHIIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQAKAKNMRVLVDGVLPDHVSAKDLILAIIGQIGTAGGTGHVIEYAGEAIRALSVEGRMTVCNMTIEGGARAGMIAPDAK
TFAYLKDRPKSPRGAAWDAAVAYWETLHSDEGAVFDREIRLDAANLPPTVTWGTSPEDVASINGVVPSPQDATSEAKKAS
IARALEYMGLKGGEKLTSIAIDRVFIGSCTNGRIEDLREAAKIVQGKHIAASVNGLVVPGSGLVKEQAEAEGLDVIFKAA
GFEWREPGCSMCLAMNPDKLAPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAGIAGHFRRHSRLALR
>Mature_469_residues
AQPRTLYDKIWDDHVVETQADGTSLLYIDRHLVHEVTSPQAFEGLRMAGRKVRAPQKTLAVVDHNVPTTDRSHGIEDPES
RIQVAQLAANAAEFGIEYFDALDHRQGVVHIIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLI
QAKAKNMRVLVDGVLPDHVSAKDLILAIIGQIGTAGGTGHVIEYAGEAIRALSVEGRMTVCNMTIEGGARAGMIAPDAKT
FAYLKDRPKSPRGAAWDAAVAYWETLHSDEGAVFDREIRLDAANLPPTVTWGTSPEDVASINGVVPSPQDATSEAKKASI
ARALEYMGLKGGEKLTSIAIDRVFIGSCTNGRIEDLREAAKIVQGKHIAASVNGLVVPGSGLVKEQAEAEGLDVIFKAAG
FEWREPGCSMCLAMNPDKLAPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAGIAGHFRRHSRLALR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=383, Percent_Identity=25.5874673629243, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=23.9473684210526, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786259, Length=457, Percent_Identity=63.2385120350109, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=23.9554317548747, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=382, Percent_Identity=26.7015706806283, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI32564738, Length=382, Percent_Identity=26.7015706806283, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI25149342, Length=300, Percent_Identity=27, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=449, Percent_Identity=25.1670378619154, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=465, Percent_Identity=66.2365591397849, Blast_Score=615, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6320440, Length=430, Percent_Identity=25.8139534883721, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=388, Percent_Identity=28.8659793814433, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322261, Length=383, Percent_Identity=27.154046997389, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI281365315, Length=382, Percent_Identity=25.6544502617801, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI17864292, Length=382, Percent_Identity=25.6544502617801, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=25.6544502617801, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28571643, Length=365, Percent_Identity=27.1232876712329, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645686, Length=378, Percent_Identity=25.3968253968254, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17137564, Length=369, Percent_Identity=24.390243902439, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50134; Mature: 50003

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPRTLYDKIWDDHVVETQADGTSLLYIDRHLVHEVTSPQAFEGLRMAGRKVRAPQKTL
CCCCHHHHHHHCCCCEEEECCCCCEEEEEEHHHHHHCCCCHHHHHHHHCCHHCCCCCHHE
AVVDHNVPTTDRSHGIEDPESRIQVAQLAANAAEFGIEYFDALDHRQGVVHIIGPEQGFT
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
LPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQAKAKNMRVLVDGVLPDHV
CCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
SAKDLILAIIGQIGTAGGTGHVIEYAGEAIRALSVEGRMTVCNMTIEGGARAGMIAPDAK
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCEEEEEEEECCCCCCCCCCCCHH
TFAYLKDRPKSPRGAAWDAAVAYWETLHSDEGAVFDREIRLDAANLPPTVTWGTSPEDVA
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEECCCCHHHHH
SINGVVPSPQDATSEAKKASIARALEYMGLKGGEKLTSIAIDRVFIGSCTNGRIEDLREA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEECCCCCHHHHHHHH
AKIVQGKHIAASVNGLVVPGSGLVKEQAEAEGLDVIFKAAGFEWREPGCSMCLAMNPDKL
HHHHCCCEEEEECCCEEECCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCC
APGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAGIAGHFRRHSRLALR
CCCHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AQPRTLYDKIWDDHVVETQADGTSLLYIDRHLVHEVTSPQAFEGLRMAGRKVRAPQKTL
CCCHHHHHHHCCCCEEEECCCCCEEEEEEHHHHHHCCCCHHHHHHHHCCHHCCCCCHHE
AVVDHNVPTTDRSHGIEDPESRIQVAQLAANAAEFGIEYFDALDHRQGVVHIIGPEQGFT
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
LPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQAKAKNMRVLVDGVLPDHV
CCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
SAKDLILAIIGQIGTAGGTGHVIEYAGEAIRALSVEGRMTVCNMTIEGGARAGMIAPDAK
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCEEEEEEEECCCCCCCCCCCCHH
TFAYLKDRPKSPRGAAWDAAVAYWETLHSDEGAVFDREIRLDAANLPPTVTWGTSPEDVA
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEECCCCHHHHH
SINGVVPSPQDATSEAKKASIARALEYMGLKGGEKLTSIAIDRVFIGSCTNGRIEDLREA
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEEEECCCCCHHHHHHHH
AKIVQGKHIAASVNGLVVPGSGLVKEQAEAEGLDVIFKAAGFEWREPGCSMCLAMNPDKL
HHHHCCCEEEEECCCEEECCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCC
APGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAGIAGHFRRHSRLALR
CCCHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA