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The map label for this gene is folC [H]

Identifier: 182678952

GI number: 182678952

Start: 2269851

End: 2271167

Strand: Reverse

Name: folC [H]

Synonym: Bind_1986

Alternate gene names: 182678952

Gene position: 2271167-2269851 (Counterclockwise)

Preceding gene: 182678953

Following gene: 182678949

Centisome position: 54.46

GC content: 60.29

Gene sequence:

>1317_bases
ATGCAGGCTCTCGACACGCTTCTTGCCCGGCTGTTCACCTTGCATCCGCAGCAGATCGAGCTTTCGCTCGGCCGTATGCG
GCGGCTCTTGGACGCCATGGGGTCGCCCGAAAAGCATTTGCCGCCGGTCATTCATGTCGCCGGGACTAATGGCAAAGGTT
CGACGGTGGCCTTTTTACGCGCCATTCTCGAGGCTGCGGGCAAAAGGGTCCATGTCTATACGTCGCCCCATCTCGTGCGG
TTTCATGAGCGGATCAGGCTCGGCGCGCCGGGCGGCGGCCGTCTTGTCGGTGATGCTGAATTATTGGAGGCTCTGCGCTT
TTGCGAGACCGTCAATAAGGGGCAACCGATCACCTTTTTCGAATCGACCACGGCGACCGCTTTCCATCTCTTTGCCAAGC
ATTCGGCAGATTACCTTTTGCTCGAGGTCGGACTCGGCGGCAGGCTGGACGCGACCAATGTTGTCGAAAATCCCCGCATG
ACGATCATCACACCGGTTTCGATCGATCATCAGGAATTCCTCGGTGAAACGCTTGAGAAGATCGCCGGTGAAAAGGCGGG
AATTCTGAAACAGGGGGTACCGGCGGTCATCGGACCGCAGCAACCGCAAGCCTTGGCCGTCATCGAGCGTGACGCGGCAC
GCTTGCATGCGCCCTTGATTCGCTCCGGCCAAGAATATGGTGTTCGCGAGGAACATGGCCATCTGATCTTCGAGGATGAA
CGCGGGCTCCTCGATTTACCCTTGCCGCGTTTGCCAGGCCGCCATCAGCATCACAATGCGGCCCAAGCGATCGCGGCGCT
TCGCTGGCTCGACGCGGATCTCCCTTTCGAAGCCATCGGACGTGGCATGGTGCAGGTGGAATGGCCGGCGAGATTGCAAA
ATCTCGCCAAGGGGCTGTTGGTGTCCCTTGCCCCGGAAGGGGCCGAACTCTGGCTCGACGGCGGGCATAATGCCGAGGGA
GGACAAGTGCTGGGTCAGGCGATGGCGGATTTCGAAGAAAAGATGCCGCGCCCGCTCGTCCTCATATGCGGTTCGCTGGC
CAGTAAGGACACGGTCGCTTTTCTCAGCGCCTTCAAAGGCTTGGCTCAAGAAGTTCTGGCTGTTCCCGTGTTGAGCGAGC
GTCATGCGCGCCCGACTAGCGAAGTCGCGGCGGCAGCGCAAAGCGCCGGTCTGTCGGCGGCAGCCTGTGAGAGCGTGAGC
TCCGCCCTGCGTTTTCTGGCTGTTCGAGATTGGCGCGTACCGCCCCGGATCTTGATCTGTGGCAGCCTCTATCTTGCGGG
CGAGGTTTTAGCGTTGAACGGCCCATCGCCAATATAG

Upstream 100 bases:

>100_bases
GGTGGTGCGCCGGCATGATCTGCCTGCCGTGCTCGCCCGTCTTTGCGCCTTTTTAACCAACAGCCCGCGTCGCGCTCCGA
TCCCCGCCTGACCCCATCGC

Downstream 100 bases:

>100_bases
ATCGTAATCCTCAATCAGAATTATCCATTAAAACAAAGAGATAGGAATTACTTCCTCACCAACTCAATAAAGAACGTCTT
CAATTCAATTGAAAATACGC

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MQALDTLLARLFTLHPQQIELSLGRMRRLLDAMGSPEKHLPPVIHVAGTNGKGSTVAFLRAILEAAGKRVHVYTSPHLVR
FHERIRLGAPGGGRLVGDAELLEALRFCETVNKGQPITFFESTTATAFHLFAKHSADYLLLEVGLGGRLDATNVVENPRM
TIITPVSIDHQEFLGETLEKIAGEKAGILKQGVPAVIGPQQPQALAVIERDAARLHAPLIRSGQEYGVREEHGHLIFEDE
RGLLDLPLPRLPGRHQHHNAAQAIAALRWLDADLPFEAIGRGMVQVEWPARLQNLAKGLLVSLAPEGAELWLDGGHNAEG
GQVLGQAMADFEEKMPRPLVLICGSLASKDTVAFLSAFKGLAQEVLAVPVLSERHARPTSEVAAAAQSAGLSAAACESVS
SALRFLAVRDWRVPPRILICGSLYLAGEVLALNGPSPI

Sequences:

>Translated_438_residues
MQALDTLLARLFTLHPQQIELSLGRMRRLLDAMGSPEKHLPPVIHVAGTNGKGSTVAFLRAILEAAGKRVHVYTSPHLVR
FHERIRLGAPGGGRLVGDAELLEALRFCETVNKGQPITFFESTTATAFHLFAKHSADYLLLEVGLGGRLDATNVVENPRM
TIITPVSIDHQEFLGETLEKIAGEKAGILKQGVPAVIGPQQPQALAVIERDAARLHAPLIRSGQEYGVREEHGHLIFEDE
RGLLDLPLPRLPGRHQHHNAAQAIAALRWLDADLPFEAIGRGMVQVEWPARLQNLAKGLLVSLAPEGAELWLDGGHNAEG
GQVLGQAMADFEEKMPRPLVLICGSLASKDTVAFLSAFKGLAQEVLAVPVLSERHARPTSEVAAAAQSAGLSAAACESVS
SALRFLAVRDWRVPPRILICGSLYLAGEVLALNGPSPI
>Mature_438_residues
MQALDTLLARLFTLHPQQIELSLGRMRRLLDAMGSPEKHLPPVIHVAGTNGKGSTVAFLRAILEAAGKRVHVYTSPHLVR
FHERIRLGAPGGGRLVGDAELLEALRFCETVNKGQPITFFESTTATAFHLFAKHSADYLLLEVGLGGRLDATNVVENPRM
TIITPVSIDHQEFLGETLEKIAGEKAGILKQGVPAVIGPQQPQALAVIERDAARLHAPLIRSGQEYGVREEHGHLIFEDE
RGLLDLPLPRLPGRHQHHNAAQAIAALRWLDADLPFEAIGRGMVQVEWPARLQNLAKGLLVSLAPEGAELWLDGGHNAEG
GQVLGQAMADFEEKMPRPLVLICGSLASKDTVAFLSAFKGLAQEVLAVPVLSERHARPTSEVAAAAQSAGLSAAACESVS
SALRFLAVRDWRVPPRILICGSLYLAGEVLALNGPSPI

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=355, Percent_Identity=32.112676056338, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI66932990, Length=355, Percent_Identity=32.112676056338, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1788654, Length=431, Percent_Identity=32.9466357308585, Blast_Score=161, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17553150, Length=471, Percent_Identity=26.3269639065817, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=471, Percent_Identity=26.3269639065817, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=471, Percent_Identity=26.3269639065817, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=437, Percent_Identity=29.9771167048055, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324815, Length=194, Percent_Identity=35.5670103092784, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24641571, Length=323, Percent_Identity=31.2693498452012, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24581568, Length=182, Percent_Identity=32.967032967033, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47171; Mature: 47171

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALDTLLARLFTLHPQQIELSLGRMRRLLDAMGSPEKHLPPVIHVAGTNGKGSTVAFLR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHH
AILEAAGKRVHVYTSPHLVRFHERIRLGAPGGGRLVGDAELLEALRFCETVNKGQPITFF
HHHHHCCCEEEEEECCHHHHHHHHHEECCCCCCCEECCHHHHHHHHHHHHHCCCCCEEEE
ESTTATAFHLFAKHSADYLLLEVGLGGRLDATNVVENPRMTIITPVSIDHQEFLGETLEK
ECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCCCEEEEEECCCCHHHHHHHHHHH
IAGEKAGILKQGVPAVIGPQQPQALAVIERDAARLHAPLIRSGQEYGVREEHGHLIFEDE
HCCCCCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
RGLLDLPLPRLPGRHQHHNAAQAIAALRWLDADLPFEAIGRGMVQVEWPARLQNLAKGLL
CCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCEEEECHHHHHHHHHHHE
VSLAPEGAELWLDGGHNAEGGQVLGQAMADFEEKMPRPLVLICGSLASKDTVAFLSAFKG
EEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
LAQEVLAVPVLSERHARPTSEVAAAAQSAGLSAAACESVSSALRFLAVRDWRVPPRILIC
HHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GSLYLAGEVLALNGPSPI
CCHHCCCEEEEECCCCCC
>Mature Secondary Structure
MQALDTLLARLFTLHPQQIELSLGRMRRLLDAMGSPEKHLPPVIHVAGTNGKGSTVAFLR
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHH
AILEAAGKRVHVYTSPHLVRFHERIRLGAPGGGRLVGDAELLEALRFCETVNKGQPITFF
HHHHHCCCEEEEEECCHHHHHHHHHEECCCCCCCEECCHHHHHHHHHHHHHCCCCCEEEE
ESTTATAFHLFAKHSADYLLLEVGLGGRLDATNVVENPRMTIITPVSIDHQEFLGETLEK
ECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCCCEEEEEECCCCHHHHHHHHHHH
IAGEKAGILKQGVPAVIGPQQPQALAVIERDAARLHAPLIRSGQEYGVREEHGHLIFEDE
HCCCCCCHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
RGLLDLPLPRLPGRHQHHNAAQAIAALRWLDADLPFEAIGRGMVQVEWPARLQNLAKGLL
CCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCEEEECHHHHHHHHHHHE
VSLAPEGAELWLDGGHNAEGGQVLGQAMADFEEKMPRPLVLICGSLASKDTVAFLSAFKG
EEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
LAQEVLAVPVLSERHARPTSEVAAAAQSAGLSAAACESVSSALRFLAVRDWRVPPRILIC
HHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GSLYLAGEVLALNGPSPI
CCHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]