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The map label for this gene is hsrA [H]

Identifier: 182678891

GI number: 182678891

Start: 2193916

End: 2195367

Strand: Direct

Name: hsrA [H]

Synonym: Bind_1924

Alternate gene names: 182678891

Gene position: 2193916-2195367 (Clockwise)

Preceding gene: 182678887

Following gene: 182678892

Centisome position: 52.61

GC content: 58.13

Gene sequence:

>1452_bases
ATGGTTTGGACGCGATTCCAGGCTTGGCCCCTGCAATCGGTTGAAACCATGCCTCGTTATCTCCTCGTCGCGCTGATCGT
GGCCTGCGCTTCCTTCATGGAAAATCTCGACGCGACGGTCATTTCGACCGCATTGCCGGCGATCGCCGCCGATCTGCATG
AAGATCCGATCGCGCTCAAGCTTGCCATGACGTCCTATCTTCTTTCACTCGCCGTCTTCATTCCGATTAGCGGCTGGGCC
GCCGATCGGTTCGGCGCCCGCACGATTTTTTGCGCCGCGATCGTCGTCTTTACGCTGGGTTCGATTCTCTGCGGCTTTTC
CGGCACCCTGCCGGAACTCATCGGCGCCCGTATCATTCAGGGCCTCGGCGGAGCCATGATGGTTCCGGTTGGCCGGCTGG
TTCTCTTGCGCAGCGTGGAACGCGCCGATCTGGTCCGGGCTTTGTCCTATCTCATGGTCCCGGCCCTGCTTGGTCCTGTG
ACGGGGCCAATCGTTGGTGGTTTCATCACCACCTATTTCCACTGGCGGTGGATTTTCTGGATCAATGTGCCGATCGGCAT
CCTCGGCTTCACCCTCGCCATGATCTTCATCGAGAATATCCGCGAAGATGAGGCAAAACCGCTCGATCGCTCTGGTTTCC
TGCTGTGCGGCCTGGGACTTGGCATGCTGATCTTCGGCTTTGCCGGCGCGGGGCGTGGACTGGTCTCCCCCTTGGCCATC
GCCTTGCTCGCCGGCTGTGGTGGGCTTTGCCTCACGCTTTACGTTCGTCATGCAAAGCGGGTCGCCCATCCGCTGATCGA
TCTGCAACTCCTCGCCATTCCGACATTCCGCGCCAGCATTGTGGGTGGCTCGCTGTTTCGCATTGGCATTGGCTCACTGC
CATTCCTTTTGCCCCTGATGCTCCAAATCGGCTTCGGAATGACCCCGTTCCAATCGGGCATGGTCACCTTCATCTCCTCC
GTCGGCGCGCTTTTGATGAAGACAACCGCGACCCCGATCCTGCGGCGCTTCGGATTTCGCTTCATCCTGATCTACGATGT
CATCCTCAGCGCGATCATTCTCACCTCTTATGGATTTTTCACCGTCTCGACCCCCATGCTCGCGATGATGAGTCTGCTCC
TCGCCGGCGGTTTTTTCCGCTCGCTGGCCTTCACCAGCATCAATGCCATTGCCTATGCCGATATCGATCATGACAGGATG
AGCCAGGCGACGAGCTTTGCCTCAGTCGCACAGCAATTATCCCTCAGTATCGGCATCGCATTTGCCGCCGGCATTCTGCA
GGGTTTGAGCTTTTTCCAGGCAGAGGGCGATCCCTTTACATTGGATAATTTCAAATGGGGTTTTATCGGCGTCGGTCTGA
TTTCAGCCGCATCCGTTTTCGTCTTTCGGACTCTCCCACAAGATGCTGGAGCGGAGCTTGCGAGCCGCCCACAGACGGAG
AGCGCTGGCTAA

Upstream 100 bases:

>100_bases
ATGCAGTAGTGCACGGGTACAGAATATCGGACGTTTAAATCGAATCCTGTCCGACGCTCTCAAATCGTTGAAAAAGCATC
AAATGGCCTAGGGGAGGTCT

Downstream 100 bases:

>100_bases
CCCCGGGCCGTTCCCCATGGCAATTCTTTAGCGTTACGACCTCTAACAGAAAGACACCTTCTCTATCTCACCCAAGCAAG
GGAAGATTGAACCTCTTTCT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MVWTRFQAWPLQSVETMPRYLLVALIVACASFMENLDATVISTALPAIAADLHEDPIALKLAMTSYLLSLAVFIPISGWA
ADRFGARTIFCAAIVVFTLGSILCGFSGTLPELIGARIIQGLGGAMMVPVGRLVLLRSVERADLVRALSYLMVPALLGPV
TGPIVGGFITTYFHWRWIFWINVPIGILGFTLAMIFIENIREDEAKPLDRSGFLLCGLGLGMLIFGFAGAGRGLVSPLAI
ALLAGCGGLCLTLYVRHAKRVAHPLIDLQLLAIPTFRASIVGGSLFRIGIGSLPFLLPLMLQIGFGMTPFQSGMVTFISS
VGALLMKTTATPILRRFGFRFILIYDVILSAIILTSYGFFTVSTPMLAMMSLLLAGGFFRSLAFTSINAIAYADIDHDRM
SQATSFASVAQQLSLSIGIAFAAGILQGLSFFQAEGDPFTLDNFKWGFIGVGLISAASVFVFRTLPQDAGAELASRPQTE
SAG

Sequences:

>Translated_483_residues
MVWTRFQAWPLQSVETMPRYLLVALIVACASFMENLDATVISTALPAIAADLHEDPIALKLAMTSYLLSLAVFIPISGWA
ADRFGARTIFCAAIVVFTLGSILCGFSGTLPELIGARIIQGLGGAMMVPVGRLVLLRSVERADLVRALSYLMVPALLGPV
TGPIVGGFITTYFHWRWIFWINVPIGILGFTLAMIFIENIREDEAKPLDRSGFLLCGLGLGMLIFGFAGAGRGLVSPLAI
ALLAGCGGLCLTLYVRHAKRVAHPLIDLQLLAIPTFRASIVGGSLFRIGIGSLPFLLPLMLQIGFGMTPFQSGMVTFISS
VGALLMKTTATPILRRFGFRFILIYDVILSAIILTSYGFFTVSTPMLAMMSLLLAGGFFRSLAFTSINAIAYADIDHDRM
SQATSFASVAQQLSLSIGIAFAAGILQGLSFFQAEGDPFTLDNFKWGFIGVGLISAASVFVFRTLPQDAGAELASRPQTE
SAG
>Mature_483_residues
MVWTRFQAWPLQSVETMPRYLLVALIVACASFMENLDATVISTALPAIAADLHEDPIALKLAMTSYLLSLAVFIPISGWA
ADRFGARTIFCAAIVVFTLGSILCGFSGTLPELIGARIIQGLGGAMMVPVGRLVLLRSVERADLVRALSYLMVPALLGPV
TGPIVGGFITTYFHWRWIFWINVPIGILGFTLAMIFIENIREDEAKPLDRSGFLLCGLGLGMLIFGFAGAGRGLVSPLAI
ALLAGCGGLCLTLYVRHAKRVAHPLIDLQLLAIPTFRASIVGGSLFRIGIGSLPFLLPLMLQIGFGMTPFQSGMVTFISS
VGALLMKTTATPILRRFGFRFILIYDVILSAIILTSYGFFTVSTPMLAMMSLLLAGGFFRSLAFTSINAIAYADIDHDRM
SQATSFASVAQQLSLSIGIAFAAGILQGLSFFQAEGDPFTLDNFKWGFIGVGLISAASVFVFRTLPQDAGAELASRPQTE
SAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=460, Percent_Identity=40, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788392, Length=485, Percent_Identity=36.4948453608247, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1789042, Length=408, Percent_Identity=26.4705882352941, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788710, Length=411, Percent_Identity=27.007299270073, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081983, Length=254, Percent_Identity=29.5275590551181, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790146, Length=175, Percent_Identity=28, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788509, Length=137, Percent_Identity=27.7372262773723, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=157, Percent_Identity=28.6624203821656, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51761; Mature: 51761

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50850 MFS ; PS00261 GLYCO_HORMONE_BETA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWTRFQAWPLQSVETMPRYLLVALIVACASFMENLDATVISTALPAIAADLHEDPIALK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHH
LAMTSYLLSLAVFIPISGWAADRFGARTIFCAAIVVFTLGSILCGFSGTLPELIGARIIQ
HHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLGGAMMVPVGRLVLLRSVERADLVRALSYLMVPALLGPVTGPIVGGFITTYFHWRWIFW
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEE
INVPIGILGFTLAMIFIENIREDEAKPLDRSGFLLCGLGLGMLIFGFAGAGRGLVSPLAI
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
ALLAGCGGLCLTLYVRHAKRVAHPLIDLQLLAIPTFRASIVGGSLFRIGIGSLPFLLPLM
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHCCCHHHHCCCCHHHHHHHH
LQIGFGMTPFQSGMVTFISSVGALLMKTTATPILRRFGFRFILIYDVILSAIILTSYGFF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHH
TVSTPMLAMMSLLLAGGFFRSLAFTSINAIAYADIDHDRMSQATSFASVAQQLSLSIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
FAAGILQGLSFFQAEGDPFTLDNFKWGFIGVGLISAASVFVFRTLPQDAGAELASRPQTE
HHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC
SAG
CCC
>Mature Secondary Structure
MVWTRFQAWPLQSVETMPRYLLVALIVACASFMENLDATVISTALPAIAADLHEDPIALK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHH
LAMTSYLLSLAVFIPISGWAADRFGARTIFCAAIVVFTLGSILCGFSGTLPELIGARIIQ
HHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLGGAMMVPVGRLVLLRSVERADLVRALSYLMVPALLGPVTGPIVGGFITTYFHWRWIFW
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEE
INVPIGILGFTLAMIFIENIREDEAKPLDRSGFLLCGLGLGMLIFGFAGAGRGLVSPLAI
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
ALLAGCGGLCLTLYVRHAKRVAHPLIDLQLLAIPTFRASIVGGSLFRIGIGSLPFLLPLM
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHCCCHHHHCCCCHHHHHHHH
LQIGFGMTPFQSGMVTFISSVGALLMKTTATPILRRFGFRFILIYDVILSAIILTSYGFF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHH
TVSTPMLAMMSLLLAGGFFRSLAFTSINAIAYADIDHDRMSQATSFASVAQQLSLSIGIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
FAAGILQGLSFFQAEGDPFTLDNFKWGFIGVGLISAASVFVFRTLPQDAGAELASRPQTE
HHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCC
SAG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]