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The map label for this gene is ispH

Identifier: 182678871

GI number: 182678871

Start: 2172525

End: 2173484

Strand: Reverse

Name: ispH

Synonym: Bind_1904

Alternate gene names: 182678871

Gene position: 2173484-2172525 (Counterclockwise)

Preceding gene: 182678875

Following gene: 182678870

Centisome position: 52.12

GC content: 58.23

Gene sequence:

>960_bases
ATGACCGATAAGCCGAAACTGCATATTCTTCTTTGCGCACCGCGCGGATTTTGTGCCGGCGTCGTGCGGGCCATTGATGC
GGTCGAACAGGCTTTGCGCCTGTACGGAGCGCCGGTCTATGTACGCCATGAGATCGTCCACAATAAATTCGTGGTCGAGG
GATTGAAGGCAAAGGGAGCAATCTTCGTCGAGGAGCTGGACGAGGTTCCAGATACGGATAAGCCCGTGATCTTCTCGGCT
CATGGCGTGCCGAAATCCATCCCCGCGGAAGCACAGAGCCGCAATATTTTCGCGATTGATGCGACCTGCCCGCTGGTGAC
AAAAGTGCATCGTGAGGCGGAACTCCACCACAATAATGGCCGCCAGGTGCTGCTCGTCGGCCATGCCGGTCACCCTGAAG
TCGTTGGCACATTGGGCCAATTGCCGGAAGGCTCGATCCTGCTGGTGCAGACGCCGGGTGAGATCGAAACTTTGCAGGTC
AAGGACGAGAAAAACCTCGCCTATGTCACGCAGACCACGCTTTCGGTCGATGACACAAGAAATATGGTCGAAGCACTGAC
CAAGCGTTTCCCCGAGATTATCGGGCCGCACCGTGAGGACATTTGCTATGCGACGACCAATCGGCAGGAGGCGGTGAAGC
GCATCGCGCCAATCGTCGATGCGCTGCTTGTCGTCGGCTCCTCCAATTCCTCCAATTCACAAAGGCTGCGGGAAGTCGCC
GAACGCTCGGGCTGCAAGCTCGCTCGTCTGGTGCTGCGTGCCGACGATGTGGAATGGGATTTGTTCTCGAATATTTCCTC
GCTTGGCATTACGGCTGGCGCCTCGGCCCCGGAAGTGCTCGTCGAGGAAATCATGGCCGCCTTCGCCGAGCGTTTCGAGC
TCGAGGTCGAGACTGTCTCGACCGCCGATGAAGGTGTCTTCTTCCCGCTCCCGCGTGAATTGCGTCAGGCGGCGTCTTGA

Upstream 100 bases:

>100_bases
GTGAACGGGGCTTCGAGATTTTCAGGATGTCCGAAGCTGCTTCATGAATGGCGTTCGTTGCGTGAATGCGTTCGAAAATC
CTGCCTGGGCAAGGCTCTCC

Downstream 100 bases:

>100_bases
CGAGCTGAGACTATTTGATGCCAAAAGGTCATCGGTACGACACCGATGACCGCCATCATAAAAATGATCAAGAGATCAGT
GCCTTTGTATGCGTGCGGTT

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTDKPKLHILLCAPRGFCAGVVRAIDAVEQALRLYGAPVYVRHEIVHNKFVVEGLKAKGAIFVEELDEVPDTDKPVIFSA
HGVPKSIPAEAQSRNIFAIDATCPLVTKVHREAELHHNNGRQVLLVGHAGHPEVVGTLGQLPEGSILLVQTPGEIETLQV
KDEKNLAYVTQTTLSVDDTRNMVEALTKRFPEIIGPHREDICYATTNRQEAVKRIAPIVDALLVVGSSNSSNSQRLREVA
ERSGCKLARLVLRADDVEWDLFSNISSLGITAGASAPEVLVEEIMAAFAERFELEVETVSTADEGVFFPLPRELRQAAS

Sequences:

>Translated_319_residues
MTDKPKLHILLCAPRGFCAGVVRAIDAVEQALRLYGAPVYVRHEIVHNKFVVEGLKAKGAIFVEELDEVPDTDKPVIFSA
HGVPKSIPAEAQSRNIFAIDATCPLVTKVHREAELHHNNGRQVLLVGHAGHPEVVGTLGQLPEGSILLVQTPGEIETLQV
KDEKNLAYVTQTTLSVDDTRNMVEALTKRFPEIIGPHREDICYATTNRQEAVKRIAPIVDALLVVGSSNSSNSQRLREVA
ERSGCKLARLVLRADDVEWDLFSNISSLGITAGASAPEVLVEEIMAAFAERFELEVETVSTADEGVFFPLPRELRQAAS
>Mature_318_residues
TDKPKLHILLCAPRGFCAGVVRAIDAVEQALRLYGAPVYVRHEIVHNKFVVEGLKAKGAIFVEELDEVPDTDKPVIFSAH
GVPKSIPAEAQSRNIFAIDATCPLVTKVHREAELHHNNGRQVLLVGHAGHPEVVGTLGQLPEGSILLVQTPGEIETLQVK
DEKNLAYVTQTTLSVDDTRNMVEALTKRFPEIIGPHREDICYATTNRQEAVKRIAPIVDALLVVGSSNSSNSQRLREVAE
RSGCKLARLVLRADDVEWDLFSNISSLGITAGASAPEVLVEEIMAAFAERFELEVETVSTADEGVFFPLPRELRQAAS

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=311, Percent_Identity=52.0900321543408, Blast_Score=331, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_BEII9 (B2IED0)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001833017.1
- GeneID:   6199867
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1904
- HOGENOM:   HBG335228
- OMA:   AAIHFKR
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34863; Mature: 34732

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKPKLHILLCAPRGFCAGVVRAIDAVEQALRLYGAPVYVRHEIVHNKFVVEGLKAKGA
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCE
IFVEELDEVPDTDKPVIFSAHGVPKSIPAEAQSRNIFAIDATCPLVTKVHREAELHHNNG
EEHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCC
RQVLLVGHAGHPEVVGTLGQLPEGSILLVQTPGEIETLQVKDEKNLAYVTQTTLSVDDTR
CEEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCEEEEEEEEEECHHHH
NMVEALTKRFPEIIGPHREDICYATTNRQEAVKRIAPIVDALLVVGSSNSSNSQRLREVA
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHH
ERSGCKLARLVLRADDVEWDLFSNISSLGITAGASAPEVLVEEIMAAFAERFELEVETVS
HHCCCHHHHHHHHCCCCCHHHHHCHHHCCEECCCCCHHHHHHHHHHHHHHHHEEEEEEEC
TADEGVFFPLPRELRQAAS
CCCCCEEECCCHHHHHHCC
>Mature Secondary Structure 
TDKPKLHILLCAPRGFCAGVVRAIDAVEQALRLYGAPVYVRHEIVHNKFVVEGLKAKGA
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCE
IFVEELDEVPDTDKPVIFSAHGVPKSIPAEAQSRNIFAIDATCPLVTKVHREAELHHNNG
EEHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCC
RQVLLVGHAGHPEVVGTLGQLPEGSILLVQTPGEIETLQVKDEKNLAYVTQTTLSVDDTR
CEEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCEEEEEEEEEECHHHH
NMVEALTKRFPEIIGPHREDICYATTNRQEAVKRIAPIVDALLVVGSSNSSNSQRLREVA
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHH
ERSGCKLARLVLRADDVEWDLFSNISSLGITAGASAPEVLVEEIMAAFAERFELEVETVS
HHCCCHHHHHHHHCCCCCHHHHHCHHHCCEECCCCCHHHHHHHHHHHHHHHHEEEEEEEC
TADEGVFFPLPRELRQAAS
CCCCCEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA