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The map label for this gene is proS

Identifier: 182678768

GI number: 182678768

Start: 2035734

End: 2037053

Strand: Reverse

Name: proS

Synonym: Bind_1797

Alternate gene names: 182678768

Gene position: 2037053-2035734 (Counterclockwise)

Preceding gene: 182678770

Following gene: 182678767

Centisome position: 48.85

GC content: 57.27

Gene sequence:

>1320_bases
ATGCGCCTTTCTCGTTATTTTCTGCCCATCCTGCGTGAAAACCCCAAGGAAGCCGAGATCGTCTCGCATCGGCTCATGCT
GCGGGCTGGCATGATCCGCCAGGAGGCGGCCGGTATTTATGCCTGGCTGCCGCTTGGTTTCCGGGTGTTGCAAAAGGTTG
AGCAGATCGTGCGGGAGGAAATGGACCGCGCCGGTGCCATCGAGCTTTTGATGCCAACGCTGCAGCTTGCCGACCTTTGG
CGTGAGACCGGGCGCTATGACGCCTATGGGCCGGAAATGCTGCGTATCAAGGATCGGCATGAGCGCGAATTGCTCTATGG
GCCGACCAATGAGGATATGATCACCGAGATTTTCCGCGCCTATGTGCGCTCCTATCGTGATCTGCCCAAAATTCTCTATC
ATATCCAATGGAAATTCCGCGACGAGCAGCGCCCTCGTTTCGGGGTCATGCGCGGCCGCGAATTTCTGATGAAGGACGCC
TATTCCTTCGATCTCGATGAAGAGGGCGCGCGGCTTTCCTACAATAAAATGTTCGTTGCCTATCTGCGCATCTATAAGCG
CATGGGCCTGACCGCCATTCCGATGCGGGCCGAGACCGGACCGATCGGCGGCGATCTCAGCCATGAGTTCATCATTCTTG
CGGATACGGGCGAATCGGCGGTCTTCTGTAACAAAGATGTGCTCGATCTTCCCATCCCCGATGAACATACCGATTATGAT
GGTGACCTGTCGCCGATCATCAAACAATGGACGAGCCTTTATGCCGCCACGGAAGATGTGCATGATGCCGCTCGTTTCGA
GAGCGAGACGCCGCCCGAGAAACGGATTGAACGCCGTGGCATCGAGGTTGGGCAAGTGTTTTATTTCGGCGAAAAATATT
CGAAGCCCATGCGATCCCTGATCACCGGGCCGGATGGCAGCGAGAAACCGTTCCAGGGCGGTTCCTATGGCGTCGGCGTG
TCGCGCCTCGTCGGCGCGATTATCGAGGCAAGCCATGACGAAGCCGGTATCATCTGGCCGGAGGCCGTCGCACCGTTCAC
GGTCGGGCTTGCCAATCTCAAGGTCGGCGATGCCGCGACTGATGCGGCCTGCGCGGAAATTTATGAACGCCTCGAGGCGC
TTGGCGTCGATGTGCTTTATGATGATACCGACGATCGTCCGGGCGGCAAATTCGCCAAGCTTGATCTGATTGGTTTGCCC
TATCAGATCATCGTCGGACCCAAGGGTCTTGCCGAGGGCAAGATCGAGATGAAGACGCGCGCGACGGGCGCACGGGAAAA
TCTATCGATTGCCGCAGTCATCGAACGGTTTACGACGTGA

Upstream 100 bases:

>100_bases
CCATGATTTCTGGTTTTGCCTCACAGGTCTTTGCCTTTCCGCGCGCCGCGCCTTAAACCTGTTACACTTAGCGTTCCTCA
CTCCTTCATGTGTCTCGTTC

Downstream 100 bases:

>100_bases
CCCAGACAAGACAAGAGTCGCCCCGGCAAGAACAGGAGAATGTATATCGCGAGGGCACCGCAGGGGCCACGCGTCCTTTC
GCGCCCTTCGAGTGGATGAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MRLSRYFLPILRENPKEAEIVSHRLMLRAGMIRQEAAGIYAWLPLGFRVLQKVEQIVREEMDRAGAIELLMPTLQLADLW
RETGRYDAYGPEMLRIKDRHERELLYGPTNEDMITEIFRAYVRSYRDLPKILYHIQWKFRDEQRPRFGVMRGREFLMKDA
YSFDLDEEGARLSYNKMFVAYLRIYKRMGLTAIPMRAETGPIGGDLSHEFIILADTGESAVFCNKDVLDLPIPDEHTDYD
GDLSPIIKQWTSLYAATEDVHDAARFESETPPEKRIERRGIEVGQVFYFGEKYSKPMRSLITGPDGSEKPFQGGSYGVGV
SRLVGAIIEASHDEAGIIWPEAVAPFTVGLANLKVGDAATDAACAEIYERLEALGVDVLYDDTDDRPGGKFAKLDLIGLP
YQIIVGPKGLAEGKIEMKTRATGARENLSIAAVIERFTT

Sequences:

>Translated_439_residues
MRLSRYFLPILRENPKEAEIVSHRLMLRAGMIRQEAAGIYAWLPLGFRVLQKVEQIVREEMDRAGAIELLMPTLQLADLW
RETGRYDAYGPEMLRIKDRHERELLYGPTNEDMITEIFRAYVRSYRDLPKILYHIQWKFRDEQRPRFGVMRGREFLMKDA
YSFDLDEEGARLSYNKMFVAYLRIYKRMGLTAIPMRAETGPIGGDLSHEFIILADTGESAVFCNKDVLDLPIPDEHTDYD
GDLSPIIKQWTSLYAATEDVHDAARFESETPPEKRIERRGIEVGQVFYFGEKYSKPMRSLITGPDGSEKPFQGGSYGVGV
SRLVGAIIEASHDEAGIIWPEAVAPFTVGLANLKVGDAATDAACAEIYERLEALGVDVLYDDTDDRPGGKFAKLDLIGLP
YQIIVGPKGLAEGKIEMKTRATGARENLSIAAVIERFTT
>Mature_439_residues
MRLSRYFLPILRENPKEAEIVSHRLMLRAGMIRQEAAGIYAWLPLGFRVLQKVEQIVREEMDRAGAIELLMPTLQLADLW
RETGRYDAYGPEMLRIKDRHERELLYGPTNEDMITEIFRAYVRSYRDLPKILYHIQWKFRDEQRPRFGVMRGREFLMKDA
YSFDLDEEGARLSYNKMFVAYLRIYKRMGLTAIPMRAETGPIGGDLSHEFIILADTGESAVFCNKDVLDLPIPDEHTDYD
GDLSPIIKQWTSLYAATEDVHDAARFESETPPEKRIERRGIEVGQVFYFGEKYSKPMRSLITGPDGSEKPFQGGSYGVGV
SRLVGAIIEASHDEAGIIWPEAVAPFTVGLANLKVGDAATDAACAEIYERLEALGVDVLYDDTDDRPGGKFAKLDLIGLP
YQIIVGPKGLAEGKIEMKTRATGARENLSIAAVIERFTT

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=437, Percent_Identity=32.4942791762014, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=57.9185520361991, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI115532348, Length=446, Percent_Identity=29.5964125560538, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=35.9550561797753, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=262, Percent_Identity=34.7328244274809, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24656200, Length=437, Percent_Identity=30.6636155606407, Blast_Score=202, Evalue=4e-52,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_BEII9 (B2IDI9)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832914.1
- GeneID:   6200401
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1797
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK12325
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49717; Mature: 49717

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFLPILRENPKEAEIVSHRLMLRAGMIRQEAAGIYAWLPLGFRVLQKVEQIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
MDRAGAIELLMPTLQLADLWRETGRYDAYGPEMLRIKDRHERELLYGPTNEDMITEIFRA
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCCHHHHHHHHHH
YVRSYRDLPKILYHIQWKFRDEQRPRFGVMRGREFLMKDAYSFDLDEEGARLSYNKMFVA
HHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
YLRIYKRMGLTAIPMRAETGPIGGDLSHEFIILADTGESAVFCNKDVLDLPIPDEHTDYD
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCEEECCCCCCCCCCC
GDLSPIIKQWTSLYAATEDVHDAARFESETPPEKRIERRGIEVGQVFYFGEKYSKPMRSL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECHHHHHHHHHH
ITGPDGSEKPFQGGSYGVGVSRLVGAIIEASHDEAGIIWPEAVAPFTVGLANLKVGDAAT
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCHHCCHHHHHHCEECCCCHH
DAACAEIYERLEALGVDVLYDDTDDRPGGKFAKLDLIGLPYQIIVGPKGLAEGKIEMKTR
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCEEEEEEC
ATGARENLSIAAVIERFTT
CCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRLSRYFLPILRENPKEAEIVSHRLMLRAGMIRQEAAGIYAWLPLGFRVLQKVEQIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHH
MDRAGAIELLMPTLQLADLWRETGRYDAYGPEMLRIKDRHERELLYGPTNEDMITEIFRA
HHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCCHHHHHHHHHH
YVRSYRDLPKILYHIQWKFRDEQRPRFGVMRGREFLMKDAYSFDLDEEGARLSYNKMFVA
HHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHH
YLRIYKRMGLTAIPMRAETGPIGGDLSHEFIILADTGESAVFCNKDVLDLPIPDEHTDYD
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCEEECCCCCCCCCCC
GDLSPIIKQWTSLYAATEDVHDAARFESETPPEKRIERRGIEVGQVFYFGEKYSKPMRSL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEECHHHHHHHHHH
ITGPDGSEKPFQGGSYGVGVSRLVGAIIEASHDEAGIIWPEAVAPFTVGLANLKVGDAAT
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCHHCCHHHHHHCEECCCCHH
DAACAEIYERLEALGVDVLYDDTDDRPGGKFAKLDLIGLPYQIIVGPKGLAEGKIEMKTR
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCEEEEEEC
ATGARENLSIAAVIERFTT
CCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA