Click here to switch to the map view.

The map label for this gene is rutF [H]

Identifier: 182678678

GI number: 182678678

Start: 1925989

End: 1926501

Strand: Reverse

Name: rutF [H]

Synonym: Bind_1705

Alternate gene names: 182678678

Gene position: 1926501-1925989 (Counterclockwise)

Preceding gene: 182678679

Following gene: 182678677

Centisome position: 46.2

GC content: 54.58

Gene sequence:

>513_bases
ATGACAACCGCAGAAACGCTTCCGGCTTCCGCCGCTCCAGACCTTGTTGCCGCTTTCAAGAATGCGATGCGGCGGCTCGC
CGCCAGTGTTACCGTCGTAACGACCAGCAAGGACAAGGAACGTTTCGGCATTACCGCGACGGCGGTGACATCAGTGACAA
CGCAGCCCCCGGCTGTTCTCGTATGCGTCAATCGCACGACAAGTGTCCATTCCATCCTTCAGTTGGGCACGCCCTTCTGT
GTCAATCTCCTGCATCACCAGCATGTCGATATTTCGAATGCCTTCGGCGGTTTTCTCAAATGCGATGATAAATTCGCTTT
TGGGAGCTGGCAGGATAACGAGCATAGCGTGCCTTTTCTCGTCGATGCCCAAGCCAATCTGTTCTGCACCGTTGATGGTC
TGTTCGATTATGGCACGCATACGATCGTCATTGGCAAGGTCGAATCGATCCTCACGACAGGCGATGTGGCACCGCTGATC
TATCAGAATGGTGGCTATTTGGGGCACGGCTGA

Upstream 100 bases:

>100_bases
CTGATCAGGATCAAAGCGACCTTCGAGGCTTTTGTAAACCGATCAATCGATCCCAAGGGGTGCTCCAGTCGGGATCAGAT
GCTTCAACGCGTGAGGGATT

Downstream 100 bases:

>100_bases
TAGCTGGTGAGGGAAAATAGCGCCGGCGGCACGATTATCCCTTGAATGAGGGATGTTGATCATTGGTTCAGCGGTCATCT
CGATGATCGCTGGTATAACG

Product: flavin reductase domain-containing protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MTTAETLPASAAPDLVAAFKNAMRRLAASVTVVTTSKDKERFGITATAVTSVTTQPPAVLVCVNRTTSVHSILQLGTPFC
VNLLHHQHVDISNAFGGFLKCDDKFAFGSWQDNEHSVPFLVDAQANLFCTVDGLFDYGTHTIVIGKVESILTTGDVAPLI
YQNGGYLGHG

Sequences:

>Translated_170_residues
MTTAETLPASAAPDLVAAFKNAMRRLAASVTVVTTSKDKERFGITATAVTSVTTQPPAVLVCVNRTTSVHSILQLGTPFC
VNLLHHQHVDISNAFGGFLKCDDKFAFGSWQDNEHSVPFLVDAQANLFCTVDGLFDYGTHTIVIGKVESILTTGDVAPLI
YQNGGYLGHG
>Mature_169_residues
TTAETLPASAAPDLVAAFKNAMRRLAASVTVVTTSKDKERFGITATAVTSVTTQPPAVLVCVNRTTSVHSILQLGTPFCV
NLLHHQHVDISNAFGGFLKCDDKFAFGSWQDNEHSVPFLVDAQANLFCTVDGLFDYGTHTIVIGKVESILTTGDVAPLIY
QNGGYLGHG

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=132, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18125; Mature: 17994

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAETLPASAAPDLVAAFKNAMRRLAASVTVVTTSKDKERFGITATAVTSVTTQPPAVL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCHHHCEEEEHEEEECCCCCEEE
VCVNRTTSVHSILQLGTPFCVNLLHHQHVDISNAFGGFLKCDDKFAFGSWQDNEHSVPFL
EEECCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCEECCCCCCCCCCCCEE
VDAQANLFCTVDGLFDYGTHTIVIGKVESILTTGDVAPLIYQNGGYLGHG
EECCCCEEEEEECCCCCCCEEEEEECHHHHHCCCCCCEEEEECCCEECCC
>Mature Secondary Structure 
TTAETLPASAAPDLVAAFKNAMRRLAASVTVVTTSKDKERFGITATAVTSVTTQPPAVL
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCHHHCEEEEHEEEECCCCCEEE
VCVNRTTSVHSILQLGTPFCVNLLHHQHVDISNAFGGFLKCDDKFAFGSWQDNEHSVPFL
EEECCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCEECCCCCCCCCCCCEE
VDAQANLFCTVDGLFDYGTHTIVIGKVESILTTGDVAPLIYQNGGYLGHG
EECCCCEEEEEECCCCCCCEEEEEECHHHHHCCCCCCEEEEECCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA