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The map label for this gene is dacD [H]

Identifier: 182678644

GI number: 182678644

Start: 1889954

End: 1891645

Strand: Direct

Name: dacD [H]

Synonym: Bind_1670

Alternate gene names: 182678644

Gene position: 1889954-1891645 (Clockwise)

Preceding gene: 182678640

Following gene: 182678651

Centisome position: 45.32

GC content: 60.52

Gene sequence:

>1692_bases
ATGTCCGATGTGGCATTTCGCCGCAGTTCATTCGCAAAGATTTTAACGCCGATTCCTTGCGGCCTCACCGCCATCGCTCT
CCTCGGCTGCATTGGATTTGGCGCAGGATTCGTGACCTCTGCCGAGGCGCGCCACCGCCATGCCCATCAGCATTCCCATT
TGGCGCGCCAAGGCTCCCATCATCACGCAAGCCATGCGGCCAGCGGCGGTGAAACACCCGCCAGTGTCGCGGCCATCGTG
GTCGACGCCAATTCCGGCCATACGCTCTATGCGCATGGTGAGCATGAATTGCGTCATCCCGCGTCAATCACCAAGGTCAT
GACGCTCTACCTCTTGTTCGAACAACTCGAGCAAGGACATTTGCGGCTCGATAGCGCGATCCACGTGTCACCGCATGCCG
CAGCACAGAAGCCGACCAAGCTTGGCCTGCATGCTGGCTCGTCGATCAGCGTTGAGGACGCGATCCGCGCCGTCGTCACG
CGATCCGCCAATGACATGGCTGTGGCTATCGCCGAAACGGTCGGGGGCGATGAAGAGACTTTTGCGGGCATGATGACCCG
CAAGGCGCATATGCTCGGCATGAGCCGGACCCATTTCGAAAATGCCTCTGGCCTGCCCAATGACGCGCAGGTCACGACCG
CGCATGATCTCGCGATCCTCGGTCGTGCGATCCAGGACCGTTTCCCGCGTTATTACCCCTATTTCTCCACGCATGTGTTC
CATTACGCCGGTCAGGAGATCCGCAATCACAATCATCTCCTCGATCAGGTCGAAGGTATGGATGGGATCAAGACGGGATA
TACGCGCGCCTCGGGCTTCAATCTCCTGACTTCGGTCAAGCGTGGTGGTCATTCCATTGTGGCTGCCGTCCTCGGCGGCG
TGAGCGCGGGCGCCCGCGACCGGATCATGGCACGGCTCATCGAAAACCAGATCGACAATGGCGCGACCACACGCTCCGTC
GGCTTGCTGACCGAAAACACAGCTTCCGACCGGCTCGCCCGTTCTGGCGAGGAACTGCCGCCGGAATTGCGTCGGATCAG
CATGCCCATGCCACCGCGCACGAGTTTGCTCGGCGATGCGAAAGCCGCAAATGCCGGCAAGCCTGACGCCGCGAATGCAC
CGATCCGCCTCGCTTCCATGAGCAATGAATTGCCCATGGAGAAGCCACGCCCGGCCTATATCGCCGGCACGGCCTCTCAA
CCAGAACATGGTCAGGCTGATCGGCCGCAAGCGGCCGCAAAGGCTGGCGATGGTTCGACCGCGCGTCTCGCAATCCAGGC
CAATACCGGCAAGGCCGCTGTCACACCGGCTGGGAACAGGGCGCAATATGCCAAGGCCGAATTGACGCAAATCCTCAAGG
CTGAAACCAGCAAGGCTGAAACAGCGAAAAAGGAGACCAGCAAAGAGAGCGAGCCGGCGTCGCCCCTCGCTCATTCGGCG
GTGGCTCCCCATGGCTGGGTCATTCAGATTGGCGCGACGGATGATGTAGCCAAGGCCACTGCTTTGCTGTCACGGGCCAA
GACGGAAGGCTCTCGAGCCCTGGCCAATGCCCAGCCCTATACGGAAAAAGTGCGCAAGGGATCGGAAACCCTCTATCGCG
CCCGCTTTGCCGGTCTCGCGGAGGAAAGCGCGGAAGCTGCGTGCAAAACTCTGAAACGTTCCGGTTTTTCGTGCTTCGCG
ACGAAAAATTGA

Upstream 100 bases:

>100_bases
TAACCAAAGCGTAACCGCGCCGCCCTACCCTTACGCCATCTCTATTCTCTTCAAATACAGCATAACCGATCATCAGCCAG
ATCGAGGGCTCAGGATGACG

Downstream 100 bases:

>100_bases
GGTCTTCAGCTGTCTCGTAAAGGAAAAATACATTCTTCCGAGACAAAAATAGAACACGATAGATAGAAGATGAAGGCTCG
GTCTTGACTGATATCATCAG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MSDVAFRRSSFAKILTPIPCGLTAIALLGCIGFGAGFVTSAEARHRHAHQHSHLARQGSHHHASHAASGGETPASVAAIV
VDANSGHTLYAHGEHELRHPASITKVMTLYLLFEQLEQGHLRLDSAIHVSPHAAAQKPTKLGLHAGSSISVEDAIRAVVT
RSANDMAVAIAETVGGDEETFAGMMTRKAHMLGMSRTHFENASGLPNDAQVTTAHDLAILGRAIQDRFPRYYPYFSTHVF
HYAGQEIRNHNHLLDQVEGMDGIKTGYTRASGFNLLTSVKRGGHSIVAAVLGGVSAGARDRIMARLIENQIDNGATTRSV
GLLTENTASDRLARSGEELPPELRRISMPMPPRTSLLGDAKAANAGKPDAANAPIRLASMSNELPMEKPRPAYIAGTASQ
PEHGQADRPQAAAKAGDGSTARLAIQANTGKAAVTPAGNRAQYAKAELTQILKAETSKAETAKKETSKESEPASPLAHSA
VAPHGWVIQIGATDDVAKATALLSRAKTEGSRALANAQPYTEKVRKGSETLYRARFAGLAEESAEAACKTLKRSGFSCFA
TKN

Sequences:

>Translated_563_residues
MSDVAFRRSSFAKILTPIPCGLTAIALLGCIGFGAGFVTSAEARHRHAHQHSHLARQGSHHHASHAASGGETPASVAAIV
VDANSGHTLYAHGEHELRHPASITKVMTLYLLFEQLEQGHLRLDSAIHVSPHAAAQKPTKLGLHAGSSISVEDAIRAVVT
RSANDMAVAIAETVGGDEETFAGMMTRKAHMLGMSRTHFENASGLPNDAQVTTAHDLAILGRAIQDRFPRYYPYFSTHVF
HYAGQEIRNHNHLLDQVEGMDGIKTGYTRASGFNLLTSVKRGGHSIVAAVLGGVSAGARDRIMARLIENQIDNGATTRSV
GLLTENTASDRLARSGEELPPELRRISMPMPPRTSLLGDAKAANAGKPDAANAPIRLASMSNELPMEKPRPAYIAGTASQ
PEHGQADRPQAAAKAGDGSTARLAIQANTGKAAVTPAGNRAQYAKAELTQILKAETSKAETAKKETSKESEPASPLAHSA
VAPHGWVIQIGATDDVAKATALLSRAKTEGSRALANAQPYTEKVRKGSETLYRARFAGLAEESAEAACKTLKRSGFSCFA
TKN
>Mature_562_residues
SDVAFRRSSFAKILTPIPCGLTAIALLGCIGFGAGFVTSAEARHRHAHQHSHLARQGSHHHASHAASGGETPASVAAIVV
DANSGHTLYAHGEHELRHPASITKVMTLYLLFEQLEQGHLRLDSAIHVSPHAAAQKPTKLGLHAGSSISVEDAIRAVVTR
SANDMAVAIAETVGGDEETFAGMMTRKAHMLGMSRTHFENASGLPNDAQVTTAHDLAILGRAIQDRFPRYYPYFSTHVFH
YAGQEIRNHNHLLDQVEGMDGIKTGYTRASGFNLLTSVKRGGHSIVAAVLGGVSAGARDRIMARLIENQIDNGATTRSVG
LLTENTASDRLARSGEELPPELRRISMPMPPRTSLLGDAKAANAGKPDAANAPIRLASMSNELPMEKPRPAYIAGTASQP
EHGQADRPQAAAKAGDGSTARLAIQANTGKAAVTPAGNRAQYAKAELTQILKAETSKAETAKKETSKESEPASPLAHSAV
APHGWVIQIGATDDVAKATALLSRAKTEGSRALANAQPYTEKVRKGSETLYRARFAGLAEESAEAACKTLKRSGFSCFAT
KN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=249, Percent_Identity=33.7349397590361, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1786851, Length=229, Percent_Identity=31.8777292576419, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1787062, Length=233, Percent_Identity=32.618025751073, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 59631; Mature: 59500

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVAFRRSSFAKILTPIPCGLTAIALLGCIGFGAGFVTSAEARHRHAHQHSHLARQGSH
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCC
HHASHAASGGETPASVAAIVVDANSGHTLYAHGEHELRHPASITKVMTLYLLFEQLEQGH
CCHHHCCCCCCCCCEEEEEEEECCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHHHHCCC
LRLDSAIHVSPHAAAQKPTKLGLHAGSSISVEDAIRAVVTRSANDMAVAIAETVGGDEET
EEECCEEEECCCHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHCCCCHHH
FAGMMTRKAHMLGMSRTHFENASGLPNDAQVTTAHDLAILGRAIQDRFPRYYPYFSTHVF
HHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHH
HYAGQEIRNHNHLLDQVEGMDGIKTGYTRASGFNLLTSVKRGGHSIVAAVLGGVSAGARD
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHH
RIMARLIENQIDNGATTRSVGLLTENTASDRLARSGEELPPELRRISMPMPPRTSLLGDA
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHCCC
KAANAGKPDAANAPIRLASMSNELPMEKPRPAYIAGTASQPEHGQADRPQAAAKAGDGST
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCE
ARLAIQANTGKAAVTPAGNRAQYAKAELTQILKAETSKAETAKKETSKESEPASPLAHSA
EEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VAPHGWVIQIGATDDVAKATALLSRAKTEGSRALANAQPYTEKVRKGSETLYRARFAGLA
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHCCHHHHHHHHHHHHH
EESAEAACKTLKRSGFSCFATKN
HHHHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
SDVAFRRSSFAKILTPIPCGLTAIALLGCIGFGAGFVTSAEARHRHAHQHSHLARQGSH
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCC
HHASHAASGGETPASVAAIVVDANSGHTLYAHGEHELRHPASITKVMTLYLLFEQLEQGH
CCHHHCCCCCCCCCEEEEEEEECCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHHHHCCC
LRLDSAIHVSPHAAAQKPTKLGLHAGSSISVEDAIRAVVTRSANDMAVAIAETVGGDEET
EEECCEEEECCCHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEHHCCCCHHH
FAGMMTRKAHMLGMSRTHFENASGLPNDAQVTTAHDLAILGRAIQDRFPRYYPYFSTHVF
HHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHH
HYAGQEIRNHNHLLDQVEGMDGIKTGYTRASGFNLLTSVKRGGHSIVAAVLGGVSAGARD
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCHHH
RIMARLIENQIDNGATTRSVGLLTENTASDRLARSGEELPPELRRISMPMPPRTSLLGDA
HHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHCCC
KAANAGKPDAANAPIRLASMSNELPMEKPRPAYIAGTASQPEHGQADRPQAAAKAGDGST
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCE
ARLAIQANTGKAAVTPAGNRAQYAKAELTQILKAETSKAETAKKETSKESEPASPLAHSA
EEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VAPHGWVIQIGATDDVAKATALLSRAKTEGSRALANAQPYTEKVRKGSETLYRARFAGLA
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHCCHHHHHHHHHHHHH
EESAEAACKTLKRSGFSCFATKN
HHHHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]