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The map label for this gene is ald [H]

Identifier: 182678545

GI number: 182678545

Start: 1773355

End: 1774476

Strand: Direct

Name: ald [H]

Synonym: Bind_1570

Alternate gene names: 182678545

Gene position: 1773355-1774476 (Clockwise)

Preceding gene: 182678541

Following gene: 182678549

Centisome position: 42.52

GC content: 62.48

Gene sequence:

>1122_bases
ATGCTGGTCGGGGTGCCGAAAGAAATCAAGGTGCATGAATATCGCGTGGGGCTGACGCCGGAGGCGGTGCGCGAATTCGT
GGCGGCGGGCAATCAGGTCATCGTCGAGACGGGAGCAGGTGCCGGCATCAATACCACCGATGCTGTCTATAAAGAGGCGG
GAGCGACGATCGTCCCGACGGCGGATGAGGTTTTCGCCAAGGCGGAAATGATCGTGAAGGTCAAGGAACCGCAACCGTCC
GAATGGGTGAAACTGCGGGAAAATCAGATTCTCTTCACTTATCTGCATCTTGCCCCCGACCCTGAGCAGGCCAAGGGACT
GCTCGCCTCGGGCGTTACCGCCATTGCCTATGAAACCGTGCTGGGGCCAGATGGGACGAGCCTGCCGCTTCTCGCGCCAA
TGAGCGAAGTGGCTGGCCGTCTTGCCATCGAGGCCGCCGGATCCGCGCTTCAGCGCCATAGCGGCGGGCGCGGCATGTTG
CTCGGCGGCGTGCCGGGTGTTCTGCCGGCCAAAGTCATGGTGCTTGGGGGCGGCGTGGTCGGCACGCATGCCGCCCGCAT
GGCGGCGGGCCTCGGCGCCGATGTGATCATTCTCGACCGTTCCCTGCCCCGCTTGCGCCAGCTTGATGAGTTGTTTCAGG
GCCGGGCCAAGACGCGCTTTGCGTCCTCCATGGCGATCGAGGAGGAAGTTCGCGCTGCTGATGTCATCATCGGCGCGGTC
CTCGTGCCGGGGGCCAGCGCACCGCGCCTGGTGACCGAAGCCATGCTGAAAACCATGAAGCCCGGCGCTGTGCTGGTTGA
TGTTGCCATCGATCAAGGCGGATGTTTCGAAACCTCGCATGCCACGACGCATGCCGCGCCGACCTATGTGGTTGATGGCA
TCGTGCATTATTGTGTGGCGAATATGCCGGGCGCCGTGCCCTATACATCGAGCCATGCCCTGAACAATGCCACCCTACCC
TATGGACTGGCGTTGGCGGCCGGTGGGATCAAGGGCATTGCCGGCCATCCAGGCCTTTTATCCGGGCTCAACGTCCATCG
TGGTCAATTGACGCATCCGGCTGTGGCCGCGAGCCTCAAGCTCGATCTTAAACCCGCTGAAAAGGCTTTGGCCGCAGGCT
AA

Upstream 100 bases:

>100_bases
ACGGCATGATGCTCGGCAATGTTGGTTCGACGCATCATGCCGGAATGTCGGAGAGGGCCGGCGGCGCTTGGCCTATCTGA
AAGGCAGGGAGTGAAGGATC

Downstream 100 bases:

>100_bases
ATCAGCCGCCATTAGGGTAAAAAGTCCGGCCTGCTTAGCAGGCCGGATTTGTCGGCCGGCGCGATCAATCCGGCTTGAAT
TGACCTTCGAAGACATAAGT

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MLVGVPKEIKVHEYRVGLTPEAVREFVAAGNQVIVETGAGAGINTTDAVYKEAGATIVPTADEVFAKAEMIVKVKEPQPS
EWVKLRENQILFTYLHLAPDPEQAKGLLASGVTAIAYETVLGPDGTSLPLLAPMSEVAGRLAIEAAGSALQRHSGGRGML
LGGVPGVLPAKVMVLGGGVVGTHAARMAAGLGADVIILDRSLPRLRQLDELFQGRAKTRFASSMAIEEEVRAADVIIGAV
LVPGASAPRLVTEAMLKTMKPGAVLVDVAIDQGGCFETSHATTHAAPTYVVDGIVHYCVANMPGAVPYTSSHALNNATLP
YGLALAAGGIKGIAGHPGLLSGLNVHRGQLTHPAVAASLKLDLKPAEKALAAG

Sequences:

>Translated_373_residues
MLVGVPKEIKVHEYRVGLTPEAVREFVAAGNQVIVETGAGAGINTTDAVYKEAGATIVPTADEVFAKAEMIVKVKEPQPS
EWVKLRENQILFTYLHLAPDPEQAKGLLASGVTAIAYETVLGPDGTSLPLLAPMSEVAGRLAIEAAGSALQRHSGGRGML
LGGVPGVLPAKVMVLGGGVVGTHAARMAAGLGADVIILDRSLPRLRQLDELFQGRAKTRFASSMAIEEEVRAADVIIGAV
LVPGASAPRLVTEAMLKTMKPGAVLVDVAIDQGGCFETSHATTHAAPTYVVDGIVHYCVANMPGAVPYTSSHALNNATLP
YGLALAAGGIKGIAGHPGLLSGLNVHRGQLTHPAVAASLKLDLKPAEKALAAG
>Mature_373_residues
MLVGVPKEIKVHEYRVGLTPEAVREFVAAGNQVIVETGAGAGINTTDAVYKEAGATIVPTADEVFAKAEMIVKVKEPQPS
EWVKLRENQILFTYLHLAPDPEQAKGLLASGVTAIAYETVLGPDGTSLPLLAPMSEVAGRLAIEAAGSALQRHSGGRGML
LGGVPGVLPAKVMVLGGGVVGTHAARMAAGLGADVIILDRSLPRLRQLDELFQGRAKTRFASSMAIEEEVRAADVIIGAV
LVPGASAPRLVTEAMLKTMKPGAVLVDVAIDQGGCFETSHATTHAAPTYVVDGIVHYCVANMPGAVPYTSSHALNNATLP
YGLALAAGGIKGIAGHPGLLSGLNVHRGQLTHPAVAASLKLDLKPAEKALAAG

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=345, Percent_Identity=31.5942028985507, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI122939153, Length=345, Percent_Identity=31.5942028985507, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1787887, Length=382, Percent_Identity=31.151832460733, Blast_Score=124, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17550456, Length=350, Percent_Identity=31.4285714285714, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 38414; Mature: 38414

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVGVPKEIKVHEYRVGLTPEAVREFVAAGNQVIVETGAGAGINTTDAVYKEAGATIVPT
CCCCCCCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCEEECC
ADEVFAKAEMIVKVKEPQPSEWVKLRENQILFTYLHLAPDPEQAKGLLASGVTAIAYETV
HHHHHHHEEEEEEECCCCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCHHHHEEEEE
LGPDGTSLPLLAPMSEVAGRLAIEAAGSALQRHSGGRGMLLGGVPGVLPAKVMVLGGGVV
ECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHEEEEECCCHH
GTHAARMAAGLGADVIILDRSLPRLRQLDELFQGRAKTRFASSMAIEEEVRAADVIIGAV
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHEEHE
LVPGASAPRLVTEAMLKTMKPGAVLVDVAIDQGGCFETSHATTHAAPTYVVDGIVHYCVA
ECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHH
NMPGAVPYTSSHALNNATLPYGLALAAGGIKGIAGHPGLLSGLNVHRGQLTHPAVAASLK
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCEEEEEE
LDLKPAEKALAAG
EECCHHHHHHCCC
>Mature Secondary Structure
MLVGVPKEIKVHEYRVGLTPEAVREFVAAGNQVIVETGAGAGINTTDAVYKEAGATIVPT
CCCCCCCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCEEECC
ADEVFAKAEMIVKVKEPQPSEWVKLRENQILFTYLHLAPDPEQAKGLLASGVTAIAYETV
HHHHHHHEEEEEEECCCCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCHHHHEEEEE
LGPDGTSLPLLAPMSEVAGRLAIEAAGSALQRHSGGRGMLLGGVPGVLPAKVMVLGGGVV
ECCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHEEEEECCCHH
GTHAARMAAGLGADVIILDRSLPRLRQLDELFQGRAKTRFASSMAIEEEVRAADVIIGAV
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHEEHE
LVPGASAPRLVTEAMLKTMKPGAVLVDVAIDQGGCFETSHATTHAAPTYVVDGIVHYCVA
ECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHH
NMPGAVPYTSSHALNNATLPYGLALAAGGIKGIAGHPGLLSGLNVHRGQLTHPAVAASLK
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCEEEEEE
LDLKPAEKALAAG
EECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]