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The map label for this gene is lipB [H]

Identifier: 182678508

GI number: 182678508

Start: 1735786

End: 1736583

Strand: Reverse

Name: lipB [H]

Synonym: Bind_1532

Alternate gene names: 182678508

Gene position: 1736583-1735786 (Counterclockwise)

Preceding gene: 182678509

Following gene: 182678507

Centisome position: 41.64

GC content: 58.4

Gene sequence:

>798_bases
ATGAATCGGCGGATCACCATTCTCGGCTGCGGCTCCTCTGGCGGCGTACCCAGGGTGGGGCAGGGGTGGGGCGCCTGCGA
TCCGAACAATCCCAAAAACCGCCGCCGCCGCTGCGCCATGCTGCTGGAACAAACCGGCACGGATGGGGGCACGACACAGA
TCCTCGTCGATATGGGCCCTGATCTGCGCGAGCAATTGCTCGATCACAACGTCAGCCATCTCGACGCGATTCTGCTAACG
CATCAGCATGCCGATCATACGCATGGCATGGATGACATCCGGCCGCTGGTCATCATGCATCATAAGCGGATCGACCTGCA
TATGGATGCAGCGACATCCGCCGATGTGCGCGAGAGCTTCGGCTATATTTTCGCAACCCCGCCCGGCAGCCAATATCCGC
CTTTGCTGACCGAGCACCGGATCATCGCAGGTGAGCCCGTTGACATTAGGGGGGCGGGCGGCGTGCTCAAAACCTTGCCG
TTCCGGCTGGAACATGGCGAGATCGATGCACTCGGCTTTCGTTTCGGCAATTTCGCCTATTCGCCGGACGTTAACGCGAT
TCCTGCGGAAAGCGTGGGATTTTTAGAGAATCTCGACCTTTGGATCGTCGATGCGCTTCGTTATACGCCGCATCCGAGCC
ATTTCTGCCTGCAGGAAACACTCGACTGGATCAAGAAGCTCAAGCCCAAGCGCGCAATCTTGACCAATCTGCACACTGAC
CTTGATTTCGAACGGCTGCGCAGCGAATTGCCGCCGCATATCGAGCCCGCCTATGACGGCATGCAGATTGTGATTTAG

Upstream 100 bases:

>100_bases
AACAGGAGATCGCCTCGGCAACGACCGCTAATGCCCTGCGGCTCTTCAGCAAAATGCCACCGCTTGCGGCAGCGGCTGGT
CACGTAGACGGGCAGGCCGC

Downstream 100 bases:

>100_bases
AGCGCATCCTCTACCAACGGGAATAACGCCAGATATCCGAGGATCCGGCAATCGACTATTTGCGCCGATGAATATAAATC
AGGACATAGGATAATCGGCC

Product: metal-dependent hydrolase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNRRITILGCGSSGGVPRVGQGWGACDPNNPKNRRRRCAMLLEQTGTDGGTTQILVDMGPDLREQLLDHNVSHLDAILLT
HQHADHTHGMDDIRPLVIMHHKRIDLHMDAATSADVRESFGYIFATPPGSQYPPLLTEHRIIAGEPVDIRGAGGVLKTLP
FRLEHGEIDALGFRFGNFAYSPDVNAIPAESVGFLENLDLWIVDALRYTPHPSHFCLQETLDWIKKLKPKRAILTNLHTD
LDFERLRSELPPHIEPAYDGMQIVI

Sequences:

>Translated_265_residues
MNRRITILGCGSSGGVPRVGQGWGACDPNNPKNRRRRCAMLLEQTGTDGGTTQILVDMGPDLREQLLDHNVSHLDAILLT
HQHADHTHGMDDIRPLVIMHHKRIDLHMDAATSADVRESFGYIFATPPGSQYPPLLTEHRIIAGEPVDIRGAGGVLKTLP
FRLEHGEIDALGFRFGNFAYSPDVNAIPAESVGFLENLDLWIVDALRYTPHPSHFCLQETLDWIKKLKPKRAILTNLHTD
LDFERLRSELPPHIEPAYDGMQIVI
>Mature_265_residues
MNRRITILGCGSSGGVPRVGQGWGACDPNNPKNRRRRCAMLLEQTGTDGGTTQILVDMGPDLREQLLDHNVSHLDAILLT
HQHADHTHGMDDIRPLVIMHHKRIDLHMDAATSADVRESFGYIFATPPGSQYPPLLTEHRIIAGEPVDIRGAGGVLKTLP
FRLEHGEIDALGFRFGNFAYSPDVNAIPAESVGFLENLDLWIVDALRYTPHPSHFCLQETLDWIKKLKPKRAILTNLHTD
LDFERLRSELPPHIEPAYDGMQIVI

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 29605; Mature: 29605

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRITILGCGSSGGVPRVGQGWGACDPNNPKNRRRRCAMLLEQTGTDGGTTQILVDMGP
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
DLREQLLDHNVSHLDAILLTHQHADHTHGMDDIRPLVIMHHKRIDLHMDAATSADVRESF
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCEEEEECCEEEEEECCCCCHHHHHHC
GYIFATPPGSQYPPLLTEHRIIAGEPVDIRGAGGVLKTLPFRLEHGEIDALGFRFGNFAY
CEEEECCCCCCCCCCHHCCEEECCCCEEECCCCCHHHHCCEEECCCCEEEEEEEECCEEE
SPDVNAIPAESVGFLENLDLWIVDALRYTPHPSHFCLQETLDWIKKLKPKRAILTNLHTD
CCCCCCCCCCCHHHHHCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCC
LDFERLRSELPPHIEPAYDGMQIVI
CCHHHHHHHCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MNRRITILGCGSSGGVPRVGQGWGACDPNNPKNRRRRCAMLLEQTGTDGGTTQILVDMGP
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
DLREQLLDHNVSHLDAILLTHQHADHTHGMDDIRPLVIMHHKRIDLHMDAATSADVRESF
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHCCEEEEECCEEEEEECCCCCHHHHHHC
GYIFATPPGSQYPPLLTEHRIIAGEPVDIRGAGGVLKTLPFRLEHGEIDALGFRFGNFAY
CEEEECCCCCCCCCCHHCCEEECCCCEEECCCCCHHHHCCEEECCCCEEEEEEEECCEEE
SPDVNAIPAESVGFLENLDLWIVDALRYTPHPSHFCLQETLDWIKKLKPKRAILTNLHTD
CCCCCCCCCCCHHHHHCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCC
LDFERLRSELPPHIEPAYDGMQIVI
CCHHHHHHHCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]