Click here to switch to the map view.

The map label for this gene is ntrC [H]

Identifier: 182678495

GI number: 182678495

Start: 1719273

End: 1720754

Strand: Direct

Name: ntrC [H]

Synonym: Bind_1519

Alternate gene names: 182678495

Gene position: 1719273-1720754 (Clockwise)

Preceding gene: 182678494

Following gene: 182678496

Centisome position: 41.23

GC content: 60.19

Gene sequence:

>1482_bases
ATGGCGGGCGGAAATATCCTCGTTGCCGATGACGATACGGCCATCCGGACAGTCCTGAGCCAAGCCCTTTCTCGCGCCGG
TTATGAAGTCCGGGCGACGGGTACGGCGGCCACACTCTGGCGCTGGGTTCAATCCGGCGACGGTGATCTTGTGATCACCG
ATGTCGTGATGCCGGATGAAAATGCCTTCGAGCTTCTTCCCAAAATGAAGAAGCTGCGGCCCGATCTGCCGATCATTGTC
ATGAGCGCGCAAAATACCTTCATGACGGCGATCAAGGCTTCGGAACGTGGCGCCTATGATTATCTGCCGAAGCCTTTCGA
CCTGCGCGAGCTCGTCTCGATCGTCGGCCGGGCGATGAGCGGCCCCAAAAATCGGGTGCATGAGGAAAAGCCCGAGGATC
TCGAGGGCATGCCTCTGGTTGGGCGCTCGCCCGCCATGCAGGAGATCTATCGGGCGCTCGCTCGTCTCATGCAGACCGAT
CTGACCGTGATGATTACCGGTGAATCCGGAACCGGCAAGGAGTTGGTCGCGCGCGCGCTGCATGATTACGGCAAGCGCAA
GAAGGGTCCCTTTGTCGCCGTCAATATGGCGGCGATCCCGCGCGACCTGATCGAAAGCGAATTGTTCGGCCATGAGAAGG
GCGCCTTCACCGGCGCTAATCAGCGCTCGCCAGGCCGCTTCGAACAGGCCGAGGGCGGCACCCTCTTTCTCGACGAAATC
GGCGATATGCCGATGGAAGCGCAGACACGGCTCCTGCGTGTCTTGCAGCAAAGTGAATATACGACAGTCGGCGGGCGCAC
GCCGATCAAGACCAATGTCCGTATCATCGCCGCCACCAATAAGGATCTGCGTGTTTCGATCCAGCAAGGCCAGTTCCGCG
AGGATCTGTTCTTCCGCCTGAATGTCGTCCCTCTGCGCTTGCCGCCCTTGCGGGAGCGCACCGAGGATATCGGTGACCTC
GCCCAGCATTTCTTCACGATTGCCGCGAGCGAAGGGCTGCCGATGAAACATATCGATCCCGAGGCGCTTGAACGGATGAA
GCGCTATCGCTGGCCGGGCAATATTCGTGAGCTCGAAAACCTGACCCGCCGTCTCGCTGCGCTTTATCCGCAGGAGACGA
TCACGGTCCAGCTTGTCGATGCCGAACTTGCCGCCGATACGACGACTTTCGCATCTCCCAATCCGCCTTTGCCGACCGCC
AATGGTCTGACGAAAAGCCGCGAGCAGGATCGTGCCATGACGCTGGCCGGTGCGATGGAGCGGCATCTTTCGGAGATCTT
CAAGGCGCATGGCGATCAATTGCCACCTCCCGGCCTTTATCACCGGGTTCTGCGCGAGCTCGAATATCCGTTGATCTCGG
CGGCGCTCAGCGCCACGCGGGGCAATCAGATCAAGGCGGCGGAACTGCTCGGCCTCAACCGCAACACCTTGCGCAAGAAG
GTCCGCGATCTCGATATTCGGCTGATGCGCTCACCACGTTGA

Upstream 100 bases:

>100_bases
GCCGCCCGGCGTCAGGCAGCGATTGCAACAAACCACCAATCAAGATGGGCGGCCTTTCCGCCTTGCATCGAGCGCATTTG
ATCCGAACAAGGAGCTTGCC

Downstream 100 bases:

>100_bases
GGCCGCCTTCGATAATCCGAGCCTTGGCCCCGCCTCGACACCGGGGCCAAGACTTTTTCATTTCCTGCGCGTTCATTCCT
TGTGCGACTATACGGCTTTG

Product: nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MAGGNILVADDDTAIRTVLSQALSRAGYEVRATGTAATLWRWVQSGDGDLVITDVVMPDENAFELLPKMKKLRPDLPIIV
MSAQNTFMTAIKASERGAYDYLPKPFDLRELVSIVGRAMSGPKNRVHEEKPEDLEGMPLVGRSPAMQEIYRALARLMQTD
LTVMITGESGTGKELVARALHDYGKRKKGPFVAVNMAAIPRDLIESELFGHEKGAFTGANQRSPGRFEQAEGGTLFLDEI
GDMPMEAQTRLLRVLQQSEYTTVGGRTPIKTNVRIIAATNKDLRVSIQQGQFREDLFFRLNVVPLRLPPLRERTEDIGDL
AQHFFTIAASEGLPMKHIDPEALERMKRYRWPGNIRELENLTRRLAALYPQETITVQLVDAELAADTTTFASPNPPLPTA
NGLTKSREQDRAMTLAGAMERHLSEIFKAHGDQLPPPGLYHRVLRELEYPLISAALSATRGNQIKAAELLGLNRNTLRKK
VRDLDIRLMRSPR

Sequences:

>Translated_493_residues
MAGGNILVADDDTAIRTVLSQALSRAGYEVRATGTAATLWRWVQSGDGDLVITDVVMPDENAFELLPKMKKLRPDLPIIV
MSAQNTFMTAIKASERGAYDYLPKPFDLRELVSIVGRAMSGPKNRVHEEKPEDLEGMPLVGRSPAMQEIYRALARLMQTD
LTVMITGESGTGKELVARALHDYGKRKKGPFVAVNMAAIPRDLIESELFGHEKGAFTGANQRSPGRFEQAEGGTLFLDEI
GDMPMEAQTRLLRVLQQSEYTTVGGRTPIKTNVRIIAATNKDLRVSIQQGQFREDLFFRLNVVPLRLPPLRERTEDIGDL
AQHFFTIAASEGLPMKHIDPEALERMKRYRWPGNIRELENLTRRLAALYPQETITVQLVDAELAADTTTFASPNPPLPTA
NGLTKSREQDRAMTLAGAMERHLSEIFKAHGDQLPPPGLYHRVLRELEYPLISAALSATRGNQIKAAELLGLNRNTLRKK
VRDLDIRLMRSPR
>Mature_492_residues
AGGNILVADDDTAIRTVLSQALSRAGYEVRATGTAATLWRWVQSGDGDLVITDVVMPDENAFELLPKMKKLRPDLPIIVM
SAQNTFMTAIKASERGAYDYLPKPFDLRELVSIVGRAMSGPKNRVHEEKPEDLEGMPLVGRSPAMQEIYRALARLMQTDL
TVMITGESGTGKELVARALHDYGKRKKGPFVAVNMAAIPRDLIESELFGHEKGAFTGANQRSPGRFEQAEGGTLFLDEIG
DMPMEAQTRLLRVLQQSEYTTVGGRTPIKTNVRIIAATNKDLRVSIQQGQFREDLFFRLNVVPLRLPPLRERTEDIGDLA
QHFFTIAASEGLPMKHIDPEALERMKRYRWPGNIRELENLTRRLAALYPQETITVQLVDAELAADTTTFASPNPPLPTAN
GLTKSREQDRAMTLAGAMERHLSEIFKAHGDQLPPPGLYHRVLRELEYPLISAALSATRGNQIKAAELLGLNRNTLRKKV
RDLDIRLMRSPR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=489, Percent_Identity=44.7852760736196, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1788550, Length=495, Percent_Identity=38.5858585858586, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1790437, Length=478, Percent_Identity=38.9121338912134, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1788905, Length=388, Percent_Identity=38.9175257731959, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI87082117, Length=241, Percent_Identity=50.6224066390041, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789233, Length=351, Percent_Identity=40.1709401709402, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789087, Length=269, Percent_Identity=46.8401486988848, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1786524, Length=281, Percent_Identity=44.8398576512456, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI87081872, Length=228, Percent_Identity=47.8070175438597, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87082152, Length=262, Percent_Identity=43.5114503816794, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=40.9691629955947, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789828, Length=298, Percent_Identity=29.8657718120805, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87081858, Length=209, Percent_Identity=33.0143540669856, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1789809, Length=109, Percent_Identity=32.1100917431193, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790552, Length=106, Percent_Identity=33.0188679245283, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786784, Length=108, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54965; Mature: 54834

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGGNILVADDDTAIRTVLSQALSRAGYEVRATGTAATLWRWVQSGDGDLVITDVVMPDE
CCCCCEEEECCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCC
NAFELLPKMKKLRPDLPIIVMSAQNTFMTAIKASERGAYDYLPKPFDLRELVSIVGRAMS
CHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHC
GPKNRVHEEKPEDLEGMPLVGRSPAMQEIYRALARLMQTDLTVMITGESGTGKELVARAL
CCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
HDYGKRKKGPFVAVNMAAIPRDLIESELFGHEKGAFTGANQRSPGRFEQAEGGTLFLDEI
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHH
GDMPMEAQTRLLRVLQQSEYTTVGGRTPIKTNVRIIAATNKDLRVSIQQGQFREDLFFRL
CCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHEEEE
NVVPLRLPPLRERTEDIGDLAQHFFTIAASEGLPMKHIDPEALERMKRYRWPGNIRELEN
EEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
LTRRLAALYPQETITVQLVDAELAADTTTFASPNPPLPTANGLTKSREQDRAMTLAGAME
HHHHHHHHCCCCEEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RHLSEIFKAHGDQLPPPGLYHRVLRELEYPLISAALSATRGNQIKAAELLGLNRNTLRKK
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHH
VRDLDIRLMRSPR
HHHHHHHHCCCCC
>Mature Secondary Structure 
AGGNILVADDDTAIRTVLSQALSRAGYEVRATGTAATLWRWVQSGDGDLVITDVVMPDE
CCCCEEEECCCHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCC
NAFELLPKMKKLRPDLPIIVMSAQNTFMTAIKASERGAYDYLPKPFDLRELVSIVGRAMS
CHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHC
GPKNRVHEEKPEDLEGMPLVGRSPAMQEIYRALARLMQTDLTVMITGESGTGKELVARAL
CCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
HDYGKRKKGPFVAVNMAAIPRDLIESELFGHEKGAFTGANQRSPGRFEQAEGGTLFLDEI
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHH
GDMPMEAQTRLLRVLQQSEYTTVGGRTPIKTNVRIIAATNKDLRVSIQQGQFREDLFFRL
CCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHEEEE
NVVPLRLPPLRERTEDIGDLAQHFFTIAASEGLPMKHIDPEALERMKRYRWPGNIRELEN
EEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
LTRRLAALYPQETITVQLVDAELAADTTTFASPNPPLPTANGLTKSREQDRAMTLAGAME
HHHHHHHHCCCCEEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RHLSEIFKAHGDQLPPPGLYHRVLRELEYPLISAALSATRGNQIKAAELLGLNRNTLRKK
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHH
VRDLDIRLMRSPR
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]