Click here to switch to the map view.

The map label for this gene is pdhC [H]

Identifier: 182678483

GI number: 182678483

Start: 1707450

End: 1708808

Strand: Direct

Name: pdhC [H]

Synonym: Bind_1507

Alternate gene names: 182678483

Gene position: 1707450-1708808 (Clockwise)

Preceding gene: 182678482

Following gene: 182678484

Centisome position: 40.94

GC content: 62.91

Gene sequence:

>1359_bases
ATGTCCATCAATATCCTCATGCCCGCGCTTTCTCCGACCATGGAGCAAGGCAAGCTCGCCAAATGGCTCAAGAAGGAAGG
CGATAAGATCAAATCGGGCGATGTCCTGGCCGAAATCGAAACCGATAAGGCGACGATGGAAGTCGAGGCCGTCGACGAAG
GCATTCTGGCCAAGATCATCATTCCCGACGGAACGGAACAGGTCGCCGTCAATACGCCGATCGCCATCATCGCCGAGGAT
GGTGAAGATGCCGCCGCCGTCGCTGCCAAAGGGGCAGGGGCACCCGCTGCGGCGCCGCCCGCCGCCGCGAGCCCGGCCGC
TGCCCCAGCGCCGGCTCCAGCACCCACCCCGGCTGCGGCTCCCGTGGCCGCTGCCGCGCCTGCAGCCGCTCCTGTGGCTG
CCAATGGTGCGCGTGTCTTTGCCTCGCCACTGGCCCGTCGTCTCGCCAAGGAAGGCGGTCTTGATCTCACAGCGGTTGCG
GGTTCGGGTCCGCATGGCCGCATCATCGAACGCGATGTGAAAGCCGCGATCGCCGCTGGACCGCAGCCGAAAGCAGCGCC
GGCTCCGGCTGCTGCTCCCGCTCCGGTGGCCGCCTCCGCTCGGGCCCCCTCGGTGGGTATGTCCGACGAGACGATCAAGA
AATTCTTTCCCGCCGGCTCCTATGAAGAGCTTCCGCATGATTCCATGCGGAAAACCATTGCCCGCCGCCTCGTCGAGGCG
AAGCAGACGATCCCGCATTTCTATCTGTCCGTCGATTGCGAACTCGATGCGCTGATGGCTTTGCGCGAGCAGGTCAATGC
CTCGGCGGCGAAGGACAAGGAGGGCAAGCCGGCCTTCAAGGTTTCGGTCAATGATTTCATCATCAAGGGCCTGGCGCTCG
CCTTGATCCAGGTGCCCGATACCAATGTGACCTGGACCGAAGGGGCCATGCTGCGCCACAAACATGCCGATGTCGGCGTG
GCCGTCTCGATCCCTGGTGGTCTCATCACGCCGATCATTCGCTCGGCCGATACCAAGAGCCTGTCGGCGATCTCCAATGA
AATGAAGGATTACGCCGCCCGCGCCAAAGCCCGCAAGTTGAAGCCGGAGGAATATCAGGGCGGCACTTCGGCGGTGTCCA
ATCTCGGCATGTTCGGCATCAAGAATTTCCAGGCGGTCATCAATCCTCCGCATGCGACAATTCTTGCGGTTGGTGCCGGC
GAGCAGCGCGTCGTTGTCAAGAACGGGGCTCCGGCTGTTGCGACCTTGATGACCGCGACGCTCTCGACCGATCACCGTGC
GGTGGACGGCGTGCTTGGCGCGCAATTGCTGGGCGCCTTCAAGAGCCTGATCGAAAACCCGATGGGCATGCTCGTTTGA

Upstream 100 bases:

>100_bases
GGCCAATCTCGAAAAACTGGCCCTGCCAAATGTCGGTGAAGTGATCGCGGCCACCAAGGCCGTCTGCTACCGCTAAACAG
CTTGCGCAAGAGGAAACCCC

Downstream 100 bases:

>100_bases
GCCTTCCCATCGTATCGATCGGAATCATGCATTTTAGGGAGGAAGGTTGCCATGGCCGCAGCCTATGACGTTCTCATCAT
TGGCGGCGGACCCGGCGGCT

Product: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSINILMPALSPTMEQGKLAKWLKKEGDKIKSGDVLAEIETDKATMEVEAVDEGILAKIIIPDGTEQVAVNTPIAIIAED
GEDAAAVAAKGAGAPAAAPPAAASPAAAPAPAPAPTPAAAPVAAAAPAAAPVAANGARVFASPLARRLAKEGGLDLTAVA
GSGPHGRIIERDVKAAIAAGPQPKAAPAPAAAPAPVAASARAPSVGMSDETIKKFFPAGSYEELPHDSMRKTIARRLVEA
KQTIPHFYLSVDCELDALMALREQVNASAAKDKEGKPAFKVSVNDFIIKGLALALIQVPDTNVTWTEGAMLRHKHADVGV
AVSIPGGLITPIIRSADTKSLSAISNEMKDYAARAKARKLKPEEYQGGTSAVSNLGMFGIKNFQAVINPPHATILAVGAG
EQRVVVKNGAPAVATLMTATLSTDHRAVDGVLGAQLLGAFKSLIENPMGMLV

Sequences:

>Translated_452_residues
MSINILMPALSPTMEQGKLAKWLKKEGDKIKSGDVLAEIETDKATMEVEAVDEGILAKIIIPDGTEQVAVNTPIAIIAED
GEDAAAVAAKGAGAPAAAPPAAASPAAAPAPAPAPTPAAAPVAAAAPAAAPVAANGARVFASPLARRLAKEGGLDLTAVA
GSGPHGRIIERDVKAAIAAGPQPKAAPAPAAAPAPVAASARAPSVGMSDETIKKFFPAGSYEELPHDSMRKTIARRLVEA
KQTIPHFYLSVDCELDALMALREQVNASAAKDKEGKPAFKVSVNDFIIKGLALALIQVPDTNVTWTEGAMLRHKHADVGV
AVSIPGGLITPIIRSADTKSLSAISNEMKDYAARAKARKLKPEEYQGGTSAVSNLGMFGIKNFQAVINPPHATILAVGAG
EQRVVVKNGAPAVATLMTATLSTDHRAVDGVLGAQLLGAFKSLIENPMGMLV
>Mature_451_residues
SINILMPALSPTMEQGKLAKWLKKEGDKIKSGDVLAEIETDKATMEVEAVDEGILAKIIIPDGTEQVAVNTPIAIIAEDG
EDAAAVAAKGAGAPAAAPPAAASPAAAPAPAPAPTPAAAPVAAAAPAAAPVAANGARVFASPLARRLAKEGGLDLTAVAG
SGPHGRIIERDVKAAIAAGPQPKAAPAPAAAPAPVAASARAPSVGMSDETIKKFFPAGSYEELPHDSMRKTIARRLVEAK
QTIPHFYLSVDCELDALMALREQVNASAAKDKEGKPAFKVSVNDFIIKGLALALIQVPDTNVTWTEGAMLRHKHADVGVA
VSIPGGLITPIIRSADTKSLSAISNEMKDYAARAKARKLKPEEYQGGTSAVSNLGMFGIKNFQAVINPPHATILAVGAGE
QRVVVKNGAPAVATLMTATLSTDHRAVDGVLGAQLLGAFKSLIENPMGMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=453, Percent_Identity=42.3841059602649, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI203098816, Length=476, Percent_Identity=36.344537815126, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI203098753, Length=476, Percent_Identity=36.5546218487395, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=42.2619047619048, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI110671329, Length=449, Percent_Identity=25.3897550111359, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786946, Length=451, Percent_Identity=29.7117516629712, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1786305, Length=427, Percent_Identity=33.0210772833724, Blast_Score=145, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=460, Percent_Identity=44.3478260869565, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=36.1904761904762, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=455, Percent_Identity=27.032967032967, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=444, Percent_Identity=25.9009009009009, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324258, Length=463, Percent_Identity=39.9568034557235, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6320352, Length=452, Percent_Identity=27.4336283185841, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321632, Length=184, Percent_Identity=33.695652173913, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129315, Length=462, Percent_Identity=43.7229437229437, Blast_Score=288, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24582497, Length=451, Percent_Identity=43.2372505543237, Blast_Score=273, Evalue=1e-73,
Organism=Drosophila melanogaster, GI18859875, Length=443, Percent_Identity=27.313769751693, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24645909, Length=235, Percent_Identity=27.6595744680851, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46220; Mature: 46088

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINILMPALSPTMEQGKLAKWLKKEGDKIKSGDVLAEIETDKATMEVEAVDEGILAKII
CCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPDGTEQVAVNTPIAIIAEDGEDAAAVAAKGAGAPAAAPPAAASPAAAPAPAPAPTPAAA
ECCCCCEEEECCCEEEEEECCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVAAAAPAAAPVAANGARVFASPLARRLAKEGGLDLTAVAGSGPHGRIIERDVKAAIAAG
CHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCC
PQPKAAPAPAAAPAPVAASARAPSVGMSDETIKKFFPAGSYEELPHDSMRKTIARRLVEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH
KQTIPHFYLSVDCELDALMALREQVNASAAKDKEGKPAFKVSVNDFIIKGLALALIQVPD
HHCCCEEEEEECCCHHHHHHHHHHHCCHHCCCCCCCCEEEEEHHHHHHHHHHEEEEECCC
TNVTWTEGAMLRHKHADVGVAVSIPGGLITPIIRSADTKSLSAISNEMKDYAARAKARKL
CCCEECCCHHHEECCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
KPEEYQGGTSAVSNLGMFGIKNFQAVINPPHATILAVGAGEQRVVVKNGAPAVATLMTAT
CCHHCCCHHHHHHHCCHHCCCCCCEEECCCCEEEEEECCCCCEEEEECCCCHHHHHHHHH
LSTDHRAVDGVLGAQLLGAFKSLIENPMGMLV
HCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCC
>Mature Secondary Structure 
SINILMPALSPTMEQGKLAKWLKKEGDKIKSGDVLAEIETDKATMEVEAVDEGILAKII
CEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPDGTEQVAVNTPIAIIAEDGEDAAAVAAKGAGAPAAAPPAAASPAAAPAPAPAPTPAAA
ECCCCCEEEECCCEEEEEECCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVAAAAPAAAPVAANGARVFASPLARRLAKEGGLDLTAVAGSGPHGRIIERDVKAAIAAG
CHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCC
PQPKAAPAPAAAPAPVAASARAPSVGMSDETIKKFFPAGSYEELPHDSMRKTIARRLVEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHH
KQTIPHFYLSVDCELDALMALREQVNASAAKDKEGKPAFKVSVNDFIIKGLALALIQVPD
HHCCCEEEEEECCCHHHHHHHHHHHCCHHCCCCCCCCEEEEEHHHHHHHHHHEEEEECCC
TNVTWTEGAMLRHKHADVGVAVSIPGGLITPIIRSADTKSLSAISNEMKDYAARAKARKL
CCCEECCCHHHEECCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
KPEEYQGGTSAVSNLGMFGIKNFQAVINPPHATILAVGAGEQRVVVKNGAPAVATLMTAT
CCHHCCCHHHHHHHCCHHCCCCCCEEECCCCEEEEEECCCCCEEEEECCCCHHHHHHHHH
LSTDHRAVDGVLGAQLLGAFKSLIENPMGMLV
HCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]