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The map label for this gene is pdhB [H]

Identifier: 182678482

GI number: 182678482

Start: 1706049

End: 1707425

Strand: Direct

Name: pdhB [H]

Synonym: Bind_1506

Alternate gene names: 182678482

Gene position: 1706049-1707425 (Clockwise)

Preceding gene: 182678481

Following gene: 182678483

Centisome position: 40.91

GC content: 61.37

Gene sequence:

>1377_bases
ATGGCGACGAATATCCTCATGCCCGCGCTTTCGCCGACCATGGAGGAAGGCAAGCTTGCGAAATGGCTGAAAAAGGAAGG
GGACCCGATCAAGTCGGGCGATATCCTGGCCGAGATCGAGACCGATAAAGCGACGATGGAAGTCGAGGCGGTCGATGAAG
GCATTCTGGCCAAAATCATCATTCCCGATGGGACTGAGCATGTGGCGGTCAATACGCCGATCGCGGTGATCGCTGGCGAC
GGCGAGGATGCCTCCGCTGTCGCCGCGCCAACTCCGGCCGCGGCGCCCGCGCCTGCCGCAGCACCCGTGGCCGAAGCGCC
GGCGGCTGCTCCGGTCGTGTCTGCGCCGCCCGCCATTGTGGTCAGCGCCGCGCCGGAAATTCCCGCTGGCACCGCCATGG
AAACGATTACGGTGCGTGAGGCCTTGAACACCGCCATGGCCGAGGAAATGCGGCGTGATCCCGATGTCTTCATCATCGGC
GAGGAAGTCGCTGAATATCAGGGCGCATACAAGATCACGCAAAATCTGTTGCAGGAATTTGGCCCGAAACGCGTCGTCGA
CACGCCGATCACAGAGCATGGCTTCGCGGGTCTCGCTGTCGGTGCGGCGCTCGCCGGCCTGAGGCCGGTCGTTGAATTCA
TGACCTTCAATTTCGCCATGCAGGCGATCGATCAAATCATCAATTCCGCTGCGAAGACACTCTATATGTCGGGCGGCCAG
ATGGGATGTCCGATCGTCTTCCGTGGCCCGAATGGCGCGGCGGCGCGGGTCGCGGCCCAGCACAGCCAGGACTATGCCGC
CTGGTATTCCCAGATTCCGGGCCTCAAGGTGGTCATGCCTTATTCGGCGGCCGATGCGAAAGGCCTGTTGAAGAGCGCCA
TCCGCGACGCCAATCCGGTGATCTTCCTCGAAAACGAGATCCTTTACGGCCATAGCTTTGAAGTGCCGAAACTCGATGAT
TTCCTGGTGCCGATCGGCAAGGCGCGTATCGCGCGGCCCGGCAAGGACGTGACCATCGTCTCTTTCGGCATCGGCATGAC
CTATGCCCTGAAAGCCGCCGAGGAACTCGCCAAGGAAGGCGTGGACGCGGAGGTGATCGACCTGCGCACCATTCGGCCGA
TGGATGTCGAGACCATTGTTGCCTCGGTCCAGAAGACCGGCCGTTGCGTCGCCGTCGAGGAAGGCTGGCCGCAATCCGGC
GTCACCGCCGAAATCGTCACGCAGCTTATGACCCATGCCTTCGATTATCTCGATGCTCCCGTCATCCGCGTAACCGGCAA
GGATGTCCCGATGCCTTATGCGGCCAATCTCGAAAAACTGGCCCTGCCAAATGTCGGTGAAGTGATCGCGGCCACCAAGG
CCGTCTGCTACCGCTAA

Upstream 100 bases:

>100_bases
GGTCTGAAAACCCTACAATAAGAATGGGCATGCGCATGGTGCGACAGGGGAGGAAGACCTTTTCCTGGCCGCGCCAGAGC
TGTGGTTTCAGGGATCAGAG

Downstream 100 bases:

>100_bases
ACAGCTTGCGCAAGAGGAAACCCCATGTCCATCAATATCCTCATGCCCGCGCTTTCTCCGACCATGGAGCAAGGCAAGCT
CGCCAAATGGCTCAAGAAGG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MATNILMPALSPTMEEGKLAKWLKKEGDPIKSGDILAEIETDKATMEVEAVDEGILAKIIIPDGTEHVAVNTPIAVIAGD
GEDASAVAAPTPAAAPAPAAAPVAEAPAAAPVVSAPPAIVVSAAPEIPAGTAMETITVREALNTAMAEEMRRDPDVFIIG
EEVAEYQGAYKITQNLLQEFGPKRVVDTPITEHGFAGLAVGAALAGLRPVVEFMTFNFAMQAIDQIINSAAKTLYMSGGQ
MGCPIVFRGPNGAAARVAAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKSAIRDANPVIFLENEILYGHSFEVPKLDD
FLVPIGKARIARPGKDVTIVSFGIGMTYALKAAEELAKEGVDAEVIDLRTIRPMDVETIVASVQKTGRCVAVEEGWPQSG
VTAEIVTQLMTHAFDYLDAPVIRVTGKDVPMPYAANLEKLALPNVGEVIAATKAVCYR

Sequences:

>Translated_458_residues
MATNILMPALSPTMEEGKLAKWLKKEGDPIKSGDILAEIETDKATMEVEAVDEGILAKIIIPDGTEHVAVNTPIAVIAGD
GEDASAVAAPTPAAAPAPAAAPVAEAPAAAPVVSAPPAIVVSAAPEIPAGTAMETITVREALNTAMAEEMRRDPDVFIIG
EEVAEYQGAYKITQNLLQEFGPKRVVDTPITEHGFAGLAVGAALAGLRPVVEFMTFNFAMQAIDQIINSAAKTLYMSGGQ
MGCPIVFRGPNGAAARVAAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKSAIRDANPVIFLENEILYGHSFEVPKLDD
FLVPIGKARIARPGKDVTIVSFGIGMTYALKAAEELAKEGVDAEVIDLRTIRPMDVETIVASVQKTGRCVAVEEGWPQSG
VTAEIVTQLMTHAFDYLDAPVIRVTGKDVPMPYAANLEKLALPNVGEVIAATKAVCYR
>Mature_457_residues
ATNILMPALSPTMEEGKLAKWLKKEGDPIKSGDILAEIETDKATMEVEAVDEGILAKIIIPDGTEHVAVNTPIAVIAGDG
EDASAVAAPTPAAAPAPAAAPVAEAPAAAPVVSAPPAIVVSAAPEIPAGTAMETITVREALNTAMAEEMRRDPDVFIIGE
EVAEYQGAYKITQNLLQEFGPKRVVDTPITEHGFAGLAVGAALAGLRPVVEFMTFNFAMQAIDQIINSAAKTLYMSGGQM
GCPIVFRGPNGAAARVAAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKSAIRDANPVIFLENEILYGHSFEVPKLDDF
LVPIGKARIARPGKDVTIVSFGIGMTYALKAAEELAKEGVDAEVIDLRTIRPMDVETIVASVQKTGRCVAVEEGWPQSGV
TAEIVTQLMTHAFDYLDAPVIRVTGKDVPMPYAANLEKLALPNVGEVIAATKAVCYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=322, Percent_Identity=61.8012422360248, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI291084858, Length=322, Percent_Identity=58.0745341614907, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI4557353, Length=323, Percent_Identity=35.9133126934984, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI34101272, Length=323, Percent_Identity=35.9133126934984, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI203098753, Length=97, Percent_Identity=48.4536082474227, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI203098816, Length=97, Percent_Identity=48.4536082474227, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI31711992, Length=85, Percent_Identity=50.5882352941176, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI260898739, Length=61, Percent_Identity=63.9344262295082, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI225637461, Length=250, Percent_Identity=29.6, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI225637459, Length=250, Percent_Identity=29.6, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI225637463, Length=250, Percent_Identity=29.6, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI205277463, Length=293, Percent_Identity=26.6211604095563, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI4507521, Length=293, Percent_Identity=26.6211604095563, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI133778974, Length=289, Percent_Identity=25.2595155709343, Blast_Score=70, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538422, Length=332, Percent_Identity=64.4578313253012, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17506935, Length=321, Percent_Identity=40.1869158878505, Blast_Score=201, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI17560088, Length=132, Percent_Identity=42.4242424242424, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17539652, Length=346, Percent_Identity=30.0578034682081, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319698, Length=331, Percent_Identity=60.4229607250755, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324258, Length=128, Percent_Identity=46.875, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321632, Length=87, Percent_Identity=45.9770114942529, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21358145, Length=328, Percent_Identity=61.890243902439, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24650940, Length=328, Percent_Identity=61.890243902439, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI160714832, Length=325, Percent_Identity=35.3846153846154, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI160714828, Length=325, Percent_Identity=35.3846153846154, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24650943, Length=94, Percent_Identity=64.8936170212766, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24650945, Length=94, Percent_Identity=64.8936170212766, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI20129315, Length=82, Percent_Identity=52.4390243902439, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45551847, Length=334, Percent_Identity=25.748502994012, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI45550715, Length=334, Percent_Identity=25.748502994012, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24645119, Length=334, Percent_Identity=25.748502994012, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24666278, Length=348, Percent_Identity=25.8620689655172, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582497, Length=75, Percent_Identity=49.3333333333333, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 48267; Mature: 48136

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATNILMPALSPTMEEGKLAKWLKKEGDPIKSGDILAEIETDKATMEVEAVDEGILAKII
CCCCEECCCCCCCHHCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPDGTEHVAVNTPIAVIAGDGEDASAVAAPTPAAAPAPAAAPVAEAPAAAPVVSAPPAIV
ECCCCCEEEECCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VSAAPEIPAGTAMETITVREALNTAMAEEMRRDPDVFIIGEEVAEYQGAYKITQNLLQEF
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHHHHC
GPKRVVDTPITEHGFAGLAVGAALAGLRPVVEFMTFNFAMQAIDQIINSAAKTLYMSGGQ
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
MGCPIVFRGPNGAAARVAAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKSAIRDANPV
CCCCEEEECCCCCHHHHHHHHCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCE
IFLENEILYGHSFEVPKLDDFLVPIGKARIARPGKDVTIVSFGIGMTYALKAAEELAKEG
EEEECEEEECCCCCCCCCCHHHCCCCCHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
VDAEVIDLRTIRPMDVETIVASVQKTGRCVAVEEGWPQSGVTAEIVTQLMTHAFDYLDAP
CCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
VIRVTGKDVPMPYAANLEKLALPNVGEVIAATKAVCYR
EEEECCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATNILMPALSPTMEEGKLAKWLKKEGDPIKSGDILAEIETDKATMEVEAVDEGILAKII
CCCEECCCCCCCHHCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEEE
IPDGTEHVAVNTPIAVIAGDGEDASAVAAPTPAAAPAPAAAPVAEAPAAAPVVSAPPAIV
ECCCCCEEEECCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VSAAPEIPAGTAMETITVREALNTAMAEEMRRDPDVFIIGEEVAEYQGAYKITQNLLQEF
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHHHHC
GPKRVVDTPITEHGFAGLAVGAALAGLRPVVEFMTFNFAMQAIDQIINSAAKTLYMSGGQ
CCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
MGCPIVFRGPNGAAARVAAQHSQDYAAWYSQIPGLKVVMPYSAADAKGLLKSAIRDANPV
CCCCEEEECCCCCHHHHHHHHCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCE
IFLENEILYGHSFEVPKLDDFLVPIGKARIARPGKDVTIVSFGIGMTYALKAAEELAKEG
EEEECEEEECCCCCCCCCCHHHCCCCCHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
VDAEVIDLRTIRPMDVETIVASVQKTGRCVAVEEGWPQSGVTAEIVTQLMTHAFDYLDAP
CCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
VIRVTGKDVPMPYAANLEKLALPNVGEVIAATKAVCYR
EEEECCCCCCCCHHCCCHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]