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The map label for this gene is gcvA [H]

Identifier: 182678409

GI number: 182678409

Start: 1637465

End: 1638385

Strand: Direct

Name: gcvA [H]

Synonym: Bind_1426

Alternate gene names: 182678409

Gene position: 1637465-1638385 (Clockwise)

Preceding gene: 182678407

Following gene: 182678410

Centisome position: 39.27

GC content: 52.99

Gene sequence:

>921_bases
ATGAAAGAGCTGCCGCCCACACCAACGTTACGAGCATTCGAGGCCGCAGCACGTCACCCTACATTCACGGCCGCATCGGC
AGAGCTGAATGTCACGCAAAGCGCTGTCAGTCATCAAATCCAGTATCTGGAAGAGCTGTGGGGGATGAAGCTATTTGAAA
GGGGAAGAACATTAAAGCTAACAACAGAGGGGAACGCGCTGGCGCCAATAATCAGACAATTTCTGGTCAGTCTTGACGCA
ACTCTAGCAGGACTACGACGGCAGCGTGATCGACACCCACTACGCATTAGCCTCACGCAGTCCTTTGCATCGCGTTGGCT
TCTTCCTCGCTTACCGAGTATCGAAGCGAGTCAACCAGACCTTCAACTTTGGATCGAAACAACTGACCACCCGATTGGTT
TTGGGCAAGGCGATGCGGACATCGCGGTACGCCTTGGCACGGGCCAATATTCGGGTCTTTTTGCTGAATTGTTATTGCGT
GAGTACATATTCCCAGTCGCAAGTAAGCAGCTTCTAGATCGTCTGGGAATGCCGAATACTCCAGCAGACTTGCTGCGCTA
TCCCCTTCTATTCCGTGCCGGCCCTGACTTGGTTCCTAAGTGGGAGTATTGGTTCGAGCGGGCTGGAGTTGATGTTTCGC
TTGCGCATTCTGGAACCTATTATCCAGACACTAACATGACAATCGAAGCCGCGCTGGCTGGATATGGGATTGCCTTGGTT
CGAAGCGGACATGTTGAGAAAGAGTTGAGGGACGGTCTGCTTATTCGTCTTCTCACCGTGCGCTACCCCTCACCGCTCGC
GTATTATTTCGTCTGTCCGAAGGGGCGGGAGCGGCTACCCGCGATCGCATCGTTCAAGGAATGGATTGTTCAGGAGGCTC
AGCAAGCACAGTCTACCTACGACAGCGAGCAAGAGATATGA

Upstream 100 bases:

>100_bases
TCCTTATCGCCGGCTGGCTCTTCTCTTCAAGCGAAACCCCTTCATTGCATCATGAGCAAACATTCATATAATACGCGAAC
CTCTATGAGCAGAGAAGCCA

Downstream 100 bases:

>100_bases
GCATTTACTCATGGATGGATGAGTATCCTTTAGTTGCTGAGAAAGTCGCCTTCTCTTTAAAGTTGGGGGGTGGGAAGGCA
GCCATCCGGATTGAATGGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKELPPTPTLRAFEAAARHPTFTAASAELNVTQSAVSHQIQYLEELWGMKLFERGRTLKLTTEGNALAPIIRQFLVSLDA
TLAGLRRQRDRHPLRISLTQSFASRWLLPRLPSIEASQPDLQLWIETTDHPIGFGQGDADIAVRLGTGQYSGLFAELLLR
EYIFPVASKQLLDRLGMPNTPADLLRYPLLFRAGPDLVPKWEYWFERAGVDVSLAHSGTYYPDTNMTIEAALAGYGIALV
RSGHVEKELRDGLLIRLLTVRYPSPLAYYFVCPKGRERLPAIASFKEWIVQEAQQAQSTYDSEQEI

Sequences:

>Translated_306_residues
MKELPPTPTLRAFEAAARHPTFTAASAELNVTQSAVSHQIQYLEELWGMKLFERGRTLKLTTEGNALAPIIRQFLVSLDA
TLAGLRRQRDRHPLRISLTQSFASRWLLPRLPSIEASQPDLQLWIETTDHPIGFGQGDADIAVRLGTGQYSGLFAELLLR
EYIFPVASKQLLDRLGMPNTPADLLRYPLLFRAGPDLVPKWEYWFERAGVDVSLAHSGTYYPDTNMTIEAALAGYGIALV
RSGHVEKELRDGLLIRLLTVRYPSPLAYYFVCPKGRERLPAIASFKEWIVQEAQQAQSTYDSEQEI
>Mature_306_residues
MKELPPTPTLRAFEAAARHPTFTAASAELNVTQSAVSHQIQYLEELWGMKLFERGRTLKLTTEGNALAPIIRQFLVSLDA
TLAGLRRQRDRHPLRISLTQSFASRWLLPRLPSIEASQPDLQLWIETTDHPIGFGQGDADIAVRLGTGQYSGLFAELLLR
EYIFPVASKQLLDRLGMPNTPADLLRYPLLFRAGPDLVPKWEYWFERAGVDVSLAHSGTYYPDTNMTIEAALAGYGIALV
RSGHVEKELRDGLLIRLLTVRYPSPLAYYFVCPKGRERLPAIASFKEWIVQEAQQAQSTYDSEQEI

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=35.3333333333333, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=33.3333333333333, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=28.0405405405405, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=28.2312925170068, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=25.5244755244755, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1786401, Length=268, Percent_Identity=25.7462686567164, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787128, Length=252, Percent_Identity=26.5873015873016, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=262, Percent_Identity=27.4809160305344, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34466; Mature: 34466

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELPPTPTLRAFEAAARHPTFTAASAELNVTQSAVSHQIQYLEELWGMKLFERGRTLKL
CCCCCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TTEGNALAPIIRQFLVSLDATLAGLRRQRDRHPLRISLTQSFASRWLLPRLPSIEASQPD
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCC
LQLWIETTDHPIGFGQGDADIAVRLGTGQYSGLFAELLLREYIFPVASKQLLDRLGMPNT
EEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PADLLRYPLLFRAGPDLVPKWEYWFERAGVDVSLAHSGTYYPDTNMTIEAALAGYGIALV
CHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCEEEEHHHHCCEEEE
RSGHVEKELRDGLLIRLLTVRYPSPLAYYFVCPKGRERLPAIASFKEWIVQEAQQAQSTY
ECCCHHHHHHCCCEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
DSEQEI
CCCCCC
>Mature Secondary Structure
MKELPPTPTLRAFEAAARHPTFTAASAELNVTQSAVSHQIQYLEELWGMKLFERGRTLKL
CCCCCCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
TTEGNALAPIIRQFLVSLDATLAGLRRQRDRHPLRISLTQSFASRWLLPRLPSIEASQPD
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCC
LQLWIETTDHPIGFGQGDADIAVRLGTGQYSGLFAELLLREYIFPVASKQLLDRLGMPNT
EEEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PADLLRYPLLFRAGPDLVPKWEYWFERAGVDVSLAHSGTYYPDTNMTIEAALAGYGIALV
CHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCEEEEHHHHCCEEEE
RSGHVEKELRDGLLIRLLTVRYPSPLAYYFVCPKGRERLPAIASFKEWIVQEAQQAQSTY
ECCCHHHHHHCCCEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHC
DSEQEI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]