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The map label for this gene is aspS

Identifier: 182678379

GI number: 182678379

Start: 1604348

End: 1606141

Strand: Direct

Name: aspS

Synonym: Bind_1396

Alternate gene names: 182678379

Gene position: 1604348-1606141 (Clockwise)

Preceding gene: 182678377

Following gene: 182678389

Centisome position: 38.47

GC content: 57.08

Gene sequence:

>1794_bases
ATGCACCGCTACCGTACCCATACTTGCGGCGATTTGCGCGAGGCCCAAGCCGACGAGACGACACGCCTTTCGGGCTGGTG
CCATCGCATCCGGGATCATGGCGGCGTCTTGTTCATCGATCTGCGCGATCATTACGGCATCACCCAATGCGTGGTTGATC
CCGATTCCAAGGCTTTTCCGCTCGCGGAAAAATTGCGCTCAGAATGGGTCGTGCGAATCGACGGACTCGTGCGTAAAAGG
CCAAGTGGCACCGAAAATCCTGAAATGCCGACCGGTTTCATCGAGATCTATGTCACCGAGATCGAGGTTTTGGGGGCGGC
AGCCGAACTGCCCATGCCGGTGTTCGGCGATATCGATTATCCAGAAGACATCCGGCTGAAATATCGCTTTCTCGACCTCC
GCCGGGAAAAGTTGCATAAAAACATTATGTTGCGTGGGCAGGTCATCGACAGCCTCCGGCGCCGTATGAAAGAGCAGGGC
TTTTTCGAATTCCAGACTCCGATCCTCACCGCTTCAAGCCCTGAGGGGGCGCGCGATTTTCTGGTGCCCTCGCGCATTCA
TGCCGGCAAATTTTACGCGCTGCCGCAGGCGCCGCAGCAATATAAGCAATTGCTGATGATGGCCGGCTTCGATCGTTATT
TCCAGATCGCGCCCTGTTTCCGCGACGAGGATCCGCGCGCCGACCGCTTGCCCGGCGAATTCTATCAGCTCGACGTCGAG
ATGAGCTTTATTACTCAGGAAGATGTCTTCGCGGCGATGGAACCCGTGATCCGGGGCGCTTTCGAGGAATTCGGCAATGG
CCAGCCCGTCACGCAGACTTTCCCGCGCATTCCCTATGCGGAAGCCATGCTCAAATATGGTTCGGACAAGCCTGATCTCC
GCAATCCCTTGGTCATCGCCGATGTGACGGATCTGTTCGCGCGCGACGACGTCAGCTTCAATGCCTTCAAGAATGTCATC
AAGAAGGGCGGTGTCGTACGCGCCATTCCCGCGACGGGGGCCGCTAGTCAACCGCGCAGTTTCTTCGACAAGCTGAACGA
TTGGGCAAAGTCAGAAGGTGCGGCGGGTCTCGGCTATGTTATTTTCGAGGGATCGGACGAAGCCCCCGTTGGCAAGGGAC
CGATTGCTAAATTTCTTCCCGCAGAAGTCCAGGCGGCAATCGTCACGCGGGCAGATCTCAAGGCTGGTGATGCGGTCTTC
TTCGCCTGCGATATCGAGGAAAAAGCCGCCAAGCTCGCCGGCGCGGCGCGTTTACGGATCGGTCATGAGCTGGGCCTTTC
GAAAACCGGCGTCTTCGAATTGTGCTGGATCGTCGATTTCCCGATGTATGAGTGGAATGAGGACGATAAAACGGTCGATT
TTTCCCATAATCCTTTCTCCATGCCGCAGGGCGGGCTTGAGGCCTTGCAGACGCAGGACCCATTGACCATCAAAGCCTTC
CAATATGACATTGCCTGCAACGGCTATGAGATCGCTTCGGGTGGTATCCGCAACCATCGCCCCGAAGCCATGGTCAAGGC
TTTCGAGATTGCCGGTTATGGCGAAGAAACCGTGGTTGAGCGTTTCGGTGGCATGTACCGGGCCTTCCAATATGGGGCTC
CCCCGCATGGTGGCATGGCGGCGGGCGTCGATCGCATCATCATGTTGCTGGCGGGCGTCCAGAACTTGCGCGAGATTTCG
CTCTTCCCGATGAACCAGAAGGCGGAAGACTTGCTGATGGGGGCACCATCCGAGGCGACCACCAAGCAATTGCGGGAATT
GCATATTCGGCTCAACCTGCCAGAGCCTAAATAA

Upstream 100 bases:

>100_bases
TGGAAGACGGCCTTGACAGATGTCTTGCCACATGCGGTTCTCCCCCGCTGAAACGGCCCATGGGCCTTGGTTTTTCCTGA
ATCCTGATCGTGATGAGATG

Downstream 100 bases:

>100_bases
GGGCGTCGGCGCGCAGCTCTCACAGATGCGGATGGGAAGCGGCCGGCCGGGCCAATGGCATGGCCGGCAAGGTCTTCCTG
TCTGCCGTCTTGCCCGATTG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MHRYRTHTCGDLREAQADETTRLSGWCHRIRDHGGVLFIDLRDHYGITQCVVDPDSKAFPLAEKLRSEWVVRIDGLVRKR
PSGTENPEMPTGFIEIYVTEIEVLGAAAELPMPVFGDIDYPEDIRLKYRFLDLRREKLHKNIMLRGQVIDSLRRRMKEQG
FFEFQTPILTASSPEGARDFLVPSRIHAGKFYALPQAPQQYKQLLMMAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFITQEDVFAAMEPVIRGAFEEFGNGQPVTQTFPRIPYAEAMLKYGSDKPDLRNPLVIADVTDLFARDDVSFNAFKNVI
KKGGVVRAIPATGAASQPRSFFDKLNDWAKSEGAAGLGYVIFEGSDEAPVGKGPIAKFLPAEVQAAIVTRADLKAGDAVF
FACDIEEKAAKLAGAARLRIGHELGLSKTGVFELCWIVDFPMYEWNEDDKTVDFSHNPFSMPQGGLEALQTQDPLTIKAF
QYDIACNGYEIASGGIRNHRPEAMVKAFEIAGYGEETVVERFGGMYRAFQYGAPPHGGMAAGVDRIIMLLAGVQNLREIS
LFPMNQKAEDLLMGAPSEATTKQLRELHIRLNLPEPK

Sequences:

>Translated_597_residues
MHRYRTHTCGDLREAQADETTRLSGWCHRIRDHGGVLFIDLRDHYGITQCVVDPDSKAFPLAEKLRSEWVVRIDGLVRKR
PSGTENPEMPTGFIEIYVTEIEVLGAAAELPMPVFGDIDYPEDIRLKYRFLDLRREKLHKNIMLRGQVIDSLRRRMKEQG
FFEFQTPILTASSPEGARDFLVPSRIHAGKFYALPQAPQQYKQLLMMAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFITQEDVFAAMEPVIRGAFEEFGNGQPVTQTFPRIPYAEAMLKYGSDKPDLRNPLVIADVTDLFARDDVSFNAFKNVI
KKGGVVRAIPATGAASQPRSFFDKLNDWAKSEGAAGLGYVIFEGSDEAPVGKGPIAKFLPAEVQAAIVTRADLKAGDAVF
FACDIEEKAAKLAGAARLRIGHELGLSKTGVFELCWIVDFPMYEWNEDDKTVDFSHNPFSMPQGGLEALQTQDPLTIKAF
QYDIACNGYEIASGGIRNHRPEAMVKAFEIAGYGEETVVERFGGMYRAFQYGAPPHGGMAAGVDRIIMLLAGVQNLREIS
LFPMNQKAEDLLMGAPSEATTKQLRELHIRLNLPEPK
>Mature_597_residues
MHRYRTHTCGDLREAQADETTRLSGWCHRIRDHGGVLFIDLRDHYGITQCVVDPDSKAFPLAEKLRSEWVVRIDGLVRKR
PSGTENPEMPTGFIEIYVTEIEVLGAAAELPMPVFGDIDYPEDIRLKYRFLDLRREKLHKNIMLRGQVIDSLRRRMKEQG
FFEFQTPILTASSPEGARDFLVPSRIHAGKFYALPQAPQQYKQLLMMAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFITQEDVFAAMEPVIRGAFEEFGNGQPVTQTFPRIPYAEAMLKYGSDKPDLRNPLVIADVTDLFARDDVSFNAFKNVI
KKGGVVRAIPATGAASQPRSFFDKLNDWAKSEGAAGLGYVIFEGSDEAPVGKGPIAKFLPAEVQAAIVTRADLKAGDAVF
FACDIEEKAAKLAGAARLRIGHELGLSKTGVFELCWIVDFPMYEWNEDDKTVDFSHNPFSMPQGGLEALQTQDPLTIKAF
QYDIACNGYEIASGGIRNHRPEAMVKAFEIAGYGEETVVERFGGMYRAFQYGAPPHGGMAAGVDRIIMLLAGVQNLREIS
LFPMNQKAEDLLMGAPSEATTKQLRELHIRLNLPEPK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=602, Percent_Identity=34.3853820598007, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI45439306, Length=326, Percent_Identity=27.9141104294479, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI226491502, Length=253, Percent_Identity=24.1106719367589, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4758762, Length=285, Percent_Identity=24.2105263157895, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788173, Length=592, Percent_Identity=41.3851351351351, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1790571, Length=220, Percent_Identity=28.6363636363636, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789256, Length=220, Percent_Identity=28.6363636363636, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32566633, Length=606, Percent_Identity=34.6534653465347, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71984122, Length=299, Percent_Identity=24.7491638795987, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=121, Percent_Identity=31.404958677686, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=645, Percent_Identity=29.922480620155, Blast_Score=217, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6321807, Length=245, Percent_Identity=27.3469387755102, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=254, Percent_Identity=27.9527559055118, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24584738, Length=608, Percent_Identity=32.7302631578947, Blast_Score=301, Evalue=8e-82,
Organism=Drosophila melanogaster, GI17136276, Length=244, Percent_Identity=29.9180327868852, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19921528, Length=232, Percent_Identity=27.1551724137931, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_BEII9 (B2IKA4)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832525.1
- GeneID:   6199281
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1396
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66893; Mature: 66893

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRTHTCGDLREAQADETTRLSGWCHRIRDHGGVLFIDLRDHYGITQCVVDPDSKAFP
CCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCC
LAEKLRSEWVVRIDGLVRKRPSGTENPEMPTGFIEIYVTEIEVLGAAAELPMPVFGDIDY
HHHHHHHHHEEEECHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHCHHHCCCCCCCCCCCC
PEDIRLKYRFLDLRREKLHKNIMLRGQVIDSLRRRMKEQGFFEFQTPILTASSPEGARDF
CCCCEEEHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCHHC
LVPSRIHAGKFYALPQAPQQYKQLLMMAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFITQEDVFAAMEPVIRGAFEEFGNGQPVTQTFPRIPYAEAMLKYGSDKPDLRNPLVIA
EEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCEEEE
DVTDLFARDDVSFNAFKNVIKKGGVVRAIPATGAASQPRSFFDKLNDWAKSEGAAGLGYV
EHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
IFEGSDEAPVGKGPIAKFLPAEVQAAIVTRADLKAGDAVFFACDIEEKAAKLAGAARLRI
EEECCCCCCCCCCCHHHHCCHHHHHHHEECCCCCCCCEEEEEECCHHHHHHHHHHHHEEE
GHELGLSKTGVFELCWIVDFPMYEWNEDDKTVDFSHNPFSMPQGGLEALQTQDPLTIKAF
HHHCCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHCCCCCEEEEEE
QYDIACNGYEIASGGIRNHRPEAMVKAFEIAGYGEETVVERFGGMYRAFQYGAPPHGGMA
EEEEEECCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCHH
AGVDRIIMLLAGVQNLREISLFPMNQKAEDLLMGAPSEATTKQLRELHIRLNLPEPK
HHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHCCCCCHHHHHHHHHEEEEECCCCCC
>Mature Secondary Structure
MHRYRTHTCGDLREAQADETTRLSGWCHRIRDHGGVLFIDLRDHYGITQCVVDPDSKAFP
CCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCCC
LAEKLRSEWVVRIDGLVRKRPSGTENPEMPTGFIEIYVTEIEVLGAAAELPMPVFGDIDY
HHHHHHHHHEEEECHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHCHHHCCCCCCCCCCCC
PEDIRLKYRFLDLRREKLHKNIMLRGQVIDSLRRRMKEQGFFEFQTPILTASSPEGARDF
CCCCEEEHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCHHC
LVPSRIHAGKFYALPQAPQQYKQLLMMAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFITQEDVFAAMEPVIRGAFEEFGNGQPVTQTFPRIPYAEAMLKYGSDKPDLRNPLVIA
EEEECHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCEEEE
DVTDLFARDDVSFNAFKNVIKKGGVVRAIPATGAASQPRSFFDKLNDWAKSEGAAGLGYV
EHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
IFEGSDEAPVGKGPIAKFLPAEVQAAIVTRADLKAGDAVFFACDIEEKAAKLAGAARLRI
EEECCCCCCCCCCCHHHHCCHHHHHHHEECCCCCCCCEEEEEECCHHHHHHHHHHHHEEE
GHELGLSKTGVFELCWIVDFPMYEWNEDDKTVDFSHNPFSMPQGGLEALQTQDPLTIKAF
HHHCCCCCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHCCCCCEEEEEE
QYDIACNGYEIASGGIRNHRPEAMVKAFEIAGYGEETVVERFGGMYRAFQYGAPPHGGMA
EEEEEECCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCHH
AGVDRIIMLLAGVQNLREISLFPMNQKAEDLLMGAPSEATTKQLRELHIRLNLPEPK
HHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHCCCCCHHHHHHHHHEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA