Click here to switch to the map view.

The map label for this gene is yafC [C]

Identifier: 182678261

GI number: 182678261

Start: 1484492

End: 1485391

Strand: Direct

Name: yafC [C]

Synonym: Bind_1277

Alternate gene names: 182678261

Gene position: 1484492-1485391 (Clockwise)

Preceding gene: 182678259

Following gene: 182678263

Centisome position: 35.6

GC content: 61.22

Gene sequence:

>900_bases
ATGAAGCTCGATGGCATCATGACATTTGTGGCGGTAGCGGAGGCAGGCTCGATTAGCGAGGCGGCCCGGCGTCTCAGCCT
TTCCAAATCGGTGGTGAGTGAGCGTTTGTCCGACCTGGAACGGAGCCTTGGCACAGTGCTCTTCCACCGCACGACACGAA
AACTTTCTCTTACCGAAGATGGCGCGGCATTTTTCGAACGCGCCGCGCGGATCACGCGCGACGTCGAAGAGGCTGCCGCT
GATTTAGCGGAGCGCCGGGGCACTTTATCCGGCCCTCTTCGCCTCTCGGCGCCGGTGACGTTTGGGCGCATGCATCTGGG
GCCGGCGCTCTATCCATTCCTGGCGCAGCACCCAAGAATCGCGATGACCTTGGAGCTCGACGACCGGCGTGTGGATGCCT
CAGCGGAGGGCTATGACGCCGTGGTACGGCACGGAAGGCTCGAGGATAGCCGCCTTATGGCCTGGTGCCTCGCATCCAGT
CGGCGCCACCTGGTCGCGTCACCGAGCTACCTCATACGTCACGGCACGCCAAAATCTTTGGCCGAGTTGGAACAGCATCG
CGGCATTTTCTACACGAATAGAGGTGCGGCTGATTGGCGTTTCATGGGGCCGGAACATGCGACGATCGTTCGAGGGACGA
TCGGCCTCCGTGTCAATAATGGTGACATGATGCGTGACGCAGCCGTGGCCGGTCTTGGTATTGCGTTGCTCCCAAGCTTC
ATTGTTGGCGCGGAGGTCAGGACTGCGGCGCTGACTGTCATCGACGTGGGGGCTGAGGTCGAGCCAGAGTTCGTGTATAT
AGCCCATCCAGAGGGGCGGCGGCCGTCGGCTAAGCTCCGCGCGCTGGCTGATTGTCTACGCAAGACTTTCGGCGATCCGC
CTTATTGGGACCGAATGTAG

Upstream 100 bases:

>100_bases
TCGCTCTGTCTCGTCGCTCTGATGCCAATGAGAATAGTCATGGTGATCACGTTCGTGCTAGGCGGCTACTAGATGATCTA
GTTTTCGGTTTAAGCGAACA

Downstream 100 bases:

>100_bases
ATCATGCCGTCATGCGCTGGTTTCGATCGGCAAGCCGGCGGCTTTCCACTCCGGATAGCCGTCTTCGAGCCGGCGCACGA
GATAGCCACGGGCTCTTAGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKLDGIMTFVAVAEAGSISEAARRLSLSKSVVSERLSDLERSLGTVLFHRTTRKLSLTEDGAAFFERAARITRDVEEAAA
DLAERRGTLSGPLRLSAPVTFGRMHLGPALYPFLAQHPRIAMTLELDDRRVDASAEGYDAVVRHGRLEDSRLMAWCLASS
RRHLVASPSYLIRHGTPKSLAELEQHRGIFYTNRGAADWRFMGPEHATIVRGTIGLRVNNGDMMRDAAVAGLGIALLPSF
IVGAEVRTAALTVIDVGAEVEPEFVYIAHPEGRRPSAKLRALADCLRKTFGDPPYWDRM

Sequences:

>Translated_299_residues
MKLDGIMTFVAVAEAGSISEAARRLSLSKSVVSERLSDLERSLGTVLFHRTTRKLSLTEDGAAFFERAARITRDVEEAAA
DLAERRGTLSGPLRLSAPVTFGRMHLGPALYPFLAQHPRIAMTLELDDRRVDASAEGYDAVVRHGRLEDSRLMAWCLASS
RRHLVASPSYLIRHGTPKSLAELEQHRGIFYTNRGAADWRFMGPEHATIVRGTIGLRVNNGDMMRDAAVAGLGIALLPSF
IVGAEVRTAALTVIDVGAEVEPEFVYIAHPEGRRPSAKLRALADCLRKTFGDPPYWDRM
>Mature_299_residues
MKLDGIMTFVAVAEAGSISEAARRLSLSKSVVSERLSDLERSLGTVLFHRTTRKLSLTEDGAAFFERAARITRDVEEAAA
DLAERRGTLSGPLRLSAPVTFGRMHLGPALYPFLAQHPRIAMTLELDDRRVDASAEGYDAVVRHGRLEDSRLMAWCLASS
RRHLVASPSYLIRHGTPKSLAELEQHRGIFYTNRGAADWRFMGPEHATIVRGTIGLRVNNGDMMRDAAVAGLGIALLPSF
IVGAEVRTAALTVIDVGAEVEPEFVYIAHPEGRRPSAKLRALADCLRKTFGDPPYWDRM

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=30.9027777777778, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.3793103448276, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=30.1369863013699, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=28.2758620689655, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789440, Length=299, Percent_Identity=26.4214046822742, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787589, Length=237, Percent_Identity=32.9113924050633, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=28.6206896551724, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=28.2352941176471, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788706, Length=240, Percent_Identity=26.25, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786448, Length=282, Percent_Identity=26.5957446808511, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=122, Percent_Identity=30.327868852459, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=128, Percent_Identity=31.25, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32891; Mature: 32891

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDGIMTFVAVAEAGSISEAARRLSLSKSVVSERLSDLERSLGTVLFHRTTRKLSLTED
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
GAAFFERAARITRDVEEAAADLAERRGTLSGPLRLSAPVTFGRMHLGPALYPFLAQHPRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECHHHHCHHHHHHHHCCCCE
AMTLELDDRRVDASAEGYDAVVRHGRLEDSRLMAWCLASSRRHLVASPSYLIRHGTPKSL
EEEEEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCCCEEECCCCHHH
AELEQHRGIFYTNRGAADWRFMGPEHATIVRGTIGLRVNNGDMMRDAAVAGLGIALLPSF
HHHHHCCCEEEECCCCCCEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHH
IVGAEVRTAALTVIDVGAEVEPEFVYIAHPEGRRPSAKLRALADCLRKTFGDPPYWDRM
HHCCCHHHHEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLDGIMTFVAVAEAGSISEAARRLSLSKSVVSERLSDLERSLGTVLFHRTTRKLSLTED
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
GAAFFERAARITRDVEEAAADLAERRGTLSGPLRLSAPVTFGRMHLGPALYPFLAQHPRI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEECHHHHCHHHHHHHHCCCCE
AMTLELDDRRVDASAEGYDAVVRHGRLEDSRLMAWCLASSRRHLVASPSYLIRHGTPKSL
EEEEEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCEEECCCCEEECCCCHHH
AELEQHRGIFYTNRGAADWRFMGPEHATIVRGTIGLRVNNGDMMRDAAVAGLGIALLPSF
HHHHHCCCEEEECCCCCCEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHH
IVGAEVRTAALTVIDVGAEVEPEFVYIAHPEGRRPSAKLRALADCLRKTFGDPPYWDRM
HHCCCHHHHEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]