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The map label for this gene is pleD [H]

Identifier: 182678198

GI number: 182678198

Start: 1407058

End: 1408431

Strand: Direct

Name: pleD [H]

Synonym: Bind_1213

Alternate gene names: 182678198

Gene position: 1407058-1408431 (Clockwise)

Preceding gene: 182678197

Following gene: 182678206

Centisome position: 33.74

GC content: 55.75

Gene sequence:

>1374_bases
ATGAGCGCGCGCATTCTCGTCGTCGATGATAACCCGTTGAACCTCAAATTGCTCGAGGCTCGGCTGACTGCTGAATATTT
CACGGTGACCACCGCCACCAATGGCTTCGACGCCCTGGCCATCTGCGAGGCTGGTGGCTGTGACATCGTGCTGCTCGATG
TCATGATGCCGGGCCTGGATGGTTTCAATGTCTGCCGTCATATCAAGCAATCGCCGAACACGATGCATATTTCTGTCGTG
ATCGTCACGTCGCTCGATCAGACAGCCGATCGGCTTGCGGGACTTGAGGCTGGCGCCGATGACTTTTTGAATAAGCCGAT
TGACGAAATCGCCTTGATTGCCCGGGTGCGATCCCTCGCGCGGCTGCATCTGGTACTCGATGAATTGCGTCGGCGCACAC
AAAATCTCGCGGCGATTGGCCTTGCTGACCTTGACGGGCTTTCAAGAATGTCAAAAGGCCACGTGCTTCTCGTCGAGGAT
CGTGTCTCGACCATCGAACGTGTGCAGGCAGCGCTTGAGGATCTTTATCCTCTCGAAATTGTAAGTGACCCTGGTCTCGT
TCTCTCGCGCGGAGAGAATAATCCTCCTGATCTCTGCATCATCAGCCTAGGCCTTGCCGATTACGACGGTTTGCGGTTGT
GCAGTCAATTACGGACTGGCGAGCGGACACGCAATAGCATGCTCCTGGCCTTGGTTGAGCGAGAAGACCGTCAGCGTCTC
CTGACGGCACTCGATCTTGGTGTGAACGATTATCTCATTCGTCCCTTCGACAATCATGAGTTGATTGCGCGAACCCGCAC
ACAAATGCTGCGCAAGCGCTATGTGGATCGGTTACGCAATACGGTTCAGGTTTCGATGGACATGGCTTTGATCGATCCTT
TGACCGGCCTGCACAATCGCCGCTTTCTGGAAGCCCAGCTCCATCGGCTGCAACAGGAACACGGCACCGCCAGCACGGTC
TTTTCTCTGATGGTTCTCGATATCGATCATTTCAAGGCGATCAATGACCGCCATGGGCATGATGCCGGCGACCAGATTTT
GAAATATTTCGCGGCACGGGTGAAGCAGGTCGTGCGGGCCGGCGATTGGCCTTGCCGGATCGGTGGCGAGGAATTTGTTG
TCCTTATGCCAGGAGCAGGGCTGGATTTCGCCAAGCAGATTGCCGAACGGGTCCGCTATGCCATCGAAAAAGACGGTTTT
GTTCTTTCGGGAACCAGAGAAAGGATTCCCGTTACTGTATCGATCGGATTGGCTGAAGCCGTGCAGCAAGGAAGCGGTGC
GGAACCTTTCGAGGCGTTGTTTCGACGCGCCGACCGGGCGCTCTACCGCGCCAAGAAGAATGGCCGCAACAGGGTCTCCG
CCGATGCAGCTTGA

Upstream 100 bases:

>100_bases
CGTCAAATTTCTGGAAACAGTGCGCAATTATCTCGCCGAGCCCTAAGGACCACCCGCTAACGATCGATAGGCGCCGCCCC
CTGGTCACAAAGGAAAAAAC

Downstream 100 bases:

>100_bases
GCTGAGCACCAGATAATGGCCTGGGGGATTAGGGCCCCAGAGGAATTGCGCATGGAATTCAGGCGTAAGCTGGCTTTTCT
TCACAAAAATTGCCGGCATT

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSARILVVDDNPLNLKLLEARLTAEYFTVTTATNGFDALAICEAGGCDIVLLDVMMPGLDGFNVCRHIKQSPNTMHISVV
IVTSLDQTADRLAGLEAGADDFLNKPIDEIALIARVRSLARLHLVLDELRRRTQNLAAIGLADLDGLSRMSKGHVLLVED
RVSTIERVQAALEDLYPLEIVSDPGLVLSRGENNPPDLCIISLGLADYDGLRLCSQLRTGERTRNSMLLALVEREDRQRL
LTALDLGVNDYLIRPFDNHELIARTRTQMLRKRYVDRLRNTVQVSMDMALIDPLTGLHNRRFLEAQLHRLQQEHGTASTV
FSLMVLDIDHFKAINDRHGHDAGDQILKYFAARVKQVVRAGDWPCRIGGEEFVVLMPGAGLDFAKQIAERVRYAIEKDGF
VLSGTRERIPVTVSIGLAEAVQQGSGAEPFEALFRRADRALYRAKKNGRNRVSADAA

Sequences:

>Translated_457_residues
MSARILVVDDNPLNLKLLEARLTAEYFTVTTATNGFDALAICEAGGCDIVLLDVMMPGLDGFNVCRHIKQSPNTMHISVV
IVTSLDQTADRLAGLEAGADDFLNKPIDEIALIARVRSLARLHLVLDELRRRTQNLAAIGLADLDGLSRMSKGHVLLVED
RVSTIERVQAALEDLYPLEIVSDPGLVLSRGENNPPDLCIISLGLADYDGLRLCSQLRTGERTRNSMLLALVEREDRQRL
LTALDLGVNDYLIRPFDNHELIARTRTQMLRKRYVDRLRNTVQVSMDMALIDPLTGLHNRRFLEAQLHRLQQEHGTASTV
FSLMVLDIDHFKAINDRHGHDAGDQILKYFAARVKQVVRAGDWPCRIGGEEFVVLMPGAGLDFAKQIAERVRYAIEKDGF
VLSGTRERIPVTVSIGLAEAVQQGSGAEPFEALFRRADRALYRAKKNGRNRVSADAA
>Mature_456_residues
SARILVVDDNPLNLKLLEARLTAEYFTVTTATNGFDALAICEAGGCDIVLLDVMMPGLDGFNVCRHIKQSPNTMHISVVI
VTSLDQTADRLAGLEAGADDFLNKPIDEIALIARVRSLARLHLVLDELRRRTQNLAAIGLADLDGLSRMSKGHVLLVEDR
VSTIERVQAALEDLYPLEIVSDPGLVLSRGENNPPDLCIISLGLADYDGLRLCSQLRTGERTRNSMLLALVEREDRQRLL
TALDLGVNDYLIRPFDNHELIARTRTQMLRKRYVDRLRNTVQVSMDMALIDPLTGLHNRRFLEAQLHRLQQEHGTASTVF
SLMVLDIDHFKAINDRHGHDAGDQILKYFAARVKQVVRAGDWPCRIGGEEFVVLMPGAGLDFAKQIAERVRYAIEKDGFV
LSGTRERIPVTVSIGLAEAVQQGSGAEPFEALFRRADRALYRAKKNGRNRVSADAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=188, Percent_Identity=40.4255319148936, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787262, Length=181, Percent_Identity=35.9116022099448, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=42.6829268292683, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI145693134, Length=218, Percent_Identity=34.4036697247706, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=35.2601156069364, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=34.5679012345679, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=33.9506172839506, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=33.7278106508876, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=37.2881355932203, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788394, Length=120, Percent_Identity=37.5, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=34.7457627118644, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786599, Length=154, Percent_Identity=30.5194805194805, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=32.5714285714286, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787229, Length=190, Percent_Identity=28.4210526315789, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082012, Length=125, Percent_Identity=29.6, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=38.8349514563107, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790552, Length=120, Percent_Identity=36.6666666666667, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789149, Length=109, Percent_Identity=33.9449541284404, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790863, Length=118, Percent_Identity=29.6610169491525, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=29.3103448275862, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789402, Length=118, Percent_Identity=33.0508474576271, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI2367329, Length=118, Percent_Identity=38.135593220339, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50803; Mature: 50672

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARILVVDDNPLNLKLLEARLTAEYFTVTTATNGFDALAICEAGGCDIVLLDVMMPGLD
CCCEEEEECCCCCCEEEEHHHHCEEEEEEEEECCCCCEEEEECCCCCCEEEEEHHCCCCC
GFNVCRHIKQSPNTMHISVVIVTSLDQTADRLAGLEAGADDFLNKPIDEIALIARVRSLA
HHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHH
RLHLVLDELRRRTQNLAAIGLADLDGLSRMSKGHVLLVEDRVSTIERVQAALEDLYPLEI
HHHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCEEE
VSDPGLVLSRGENNPPDLCIISLGLADYDGLRLCSQLRTGERTRNSMLLALVEREDRQRL
ECCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHCEEEEEECHHHHHHH
LTALDLGVNDYLIRPFDNHELIARTRTQMLRKRYVDRLRNTVQVSMDMALIDPLTGLHNR
HHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RFLEAQLHRLQQEHGTASTVFSLMVLDIDHFKAINDRHGHDAGDQILKYFAARVKQVVRA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
GDWPCRIGGEEFVVLMPGAGLDFAKQIAERVRYAIEKDGFVLSGTRERIPVTVSIGLAEA
CCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECHHHH
VQQGSGAEPFEALFRRADRALYRAKKNGRNRVSADAA
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SARILVVDDNPLNLKLLEARLTAEYFTVTTATNGFDALAICEAGGCDIVLLDVMMPGLD
CCEEEEECCCCCCEEEEHHHHCEEEEEEEEECCCCCEEEEECCCCCCEEEEEHHCCCCC
GFNVCRHIKQSPNTMHISVVIVTSLDQTADRLAGLEAGADDFLNKPIDEIALIARVRSLA
HHHHHHHHHCCCCEEEEEEEEEECCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHH
RLHLVLDELRRRTQNLAAIGLADLDGLSRMSKGHVLLVEDRVSTIERVQAALEDLYPLEI
HHHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCEEE
VSDPGLVLSRGENNPPDLCIISLGLADYDGLRLCSQLRTGERTRNSMLLALVEREDRQRL
ECCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHCEEEEEECHHHHHHH
LTALDLGVNDYLIRPFDNHELIARTRTQMLRKRYVDRLRNTVQVSMDMALIDPLTGLHNR
HHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RFLEAQLHRLQQEHGTASTVFSLMVLDIDHFKAINDRHGHDAGDQILKYFAARVKQVVRA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
GDWPCRIGGEEFVVLMPGAGLDFAKQIAERVRYAIEKDGFVLSGTRERIPVTVSIGLAEA
CCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECHHHH
VQQGSGAEPFEALFRRADRALYRAKKNGRNRVSADAA
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA