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The map label for this gene is hybC [C]

Identifier: 182678132

GI number: 182678132

Start: 1327796

End: 1329262

Strand: Direct

Name: hybC [C]

Synonym: Bind_1147

Alternate gene names: 182678132

Gene position: 1327796-1329262 (Clockwise)

Preceding gene: 182678131

Following gene: 182678133

Centisome position: 31.84

GC content: 61.83

Gene sequence:

>1467_bases
ATGAGCGAGGCAGCGACGCGCCTGATTGTGGGACCGTTCAACCGTGTCGAGGGTGATCTCGAAGTGCGGCTGGAGATGGA
AGATGGCGTTGTGACAGCCGCTTTCGTCAATGCCACATTGTTCCGGGGCTTCGAGCGCATCCTCGAAGGACGCGACCCGA
GCGATGCCTTGGTGATTGCACCACGTATCTGCGGCATATGTTCGGTCTCGCAATCGCATGCGGCGGCCTTGGCCTTGGCG
GGAGTTATGGGGATCGCGCCCGCGCGCAATGGGCAGATCGCCGCCAATATCACTCTTGCCACCGAAAATCTGGCCGATCA
TCTGACGCATTTTCATTTGTTCTTCATGCCTGATTTCGCGCGGGGACCCTATCGCGACCGGCCCTGGTTCGACCGCGCCG
AGGCCCGTTTCGCCGCCTTGCGTGGGGCATCCGTGCGGCCGATGATGCAGACCCGCGCGTTGCTTTTGCATGTCATGGGG
CTGATCGCCGGACATTGGCCGCATACATTGGCGATCCAGCCTGGCGGTTTTTCTCGCGGGGTTGATGCGCGTGACAAAAT
CAAGCTCCTGGCCATTCTTTCAGGGGTCCGCGCCGCCTTGGAAGAGCGTTTGTTTGGCACGGCTCTGGAGCGCGTTGCGG
CACTTGCCGACCCCGAAGAACTTTCTGCCTGGCGTATGCATGGGCCGGAAGGGGATTTCCGCCTGTTTCTGGAGATCGCG
GCCGATCTCCATCTCGAAACACTCGGGCGCGCCTATGATCGTTTCCTCTGTTACGGCGCCTATCCTGGTGGCGGTGCGGA
CGGCGCACCGCTCTATCCTTCGGGCCTCTTCGCGCATGGATTGGAGCATCCGCTTGATCTCGAGGCCATAGCGGAGGATC
ACACCTATGCGCGAATGGAAAACCGTGGCCTCATGCATCCCCCCTTTATGGGATCGACCTCACCGGATGGCGCGGATGAA
CAAGGCTATAGCTGGTGCAAGGCTCCGCGTTTGTCGAACGAGCCTTGTGAAACCGGAGCCTTGGCGCGACAGGTGATGGA
TGGCCAGCCTCTTGTGCGCGCCTTGGTGGCCCGCGCGGGGGGTAATGTCTTCAGCCGTGTCATTGCGCGGCTTTTGGAAG
TGGCGCGTGTCCTGATCCAGATGGAAACATGGACCAAAGCGTTGGAGCCCGGCGCGCCCTGGTGTGTTCCTGCCGGGACG
ATCGGTACGGGACGCGCGGTCGGCTTGACGGAAGCTGCACGCGGCGCCTTGGGCCATTGGCTGCGGGTCGAAAACGGGCG
GATCGCTGGTTATCAGATCATTGCGCCTACGACCTGGAATTTCTCGCCGCGCGACGCGCGAGGCCTGCCCGGTCCCTTGG
AACGTGCCTTGATCGGTGCGCCCGTTCAGTCCGGCGAGACCACGCCAGTTTCCGTGCAGCATATCGTGCGCTCGTTTGAT
CCCTGCATGGCTTGCACGGTGCATTGA

Upstream 100 bases:

>100_bases
GCCAAGGCCGCAACGCCGGAACGCCTCGCGCGCAATGCGACTAGCGATCACGTCCTGATCCCGCCCGTGACGCGCGTGCC
GCGACGGAGCTAGCGAGAGT

Downstream 100 bases:

>100_bases
GCCCGTCTGATGGAGACAATTGAGACCGAAATTTTATCTGTCACGGGATTGGTGCAGGGGGTTGGTTTTCGCCCCTTCGT
CTGGCGTCTCGCGCAAAGGC

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSEAATRLIVGPFNRVEGDLEVRLEMEDGVVTAAFVNATLFRGFERILEGRDPSDALVIAPRICGICSVSQSHAAALALA
GVMGIAPARNGQIAANITLATENLADHLTHFHLFFMPDFARGPYRDRPWFDRAEARFAALRGASVRPMMQTRALLLHVMG
LIAGHWPHTLAIQPGGFSRGVDARDKIKLLAILSGVRAALEERLFGTALERVAALADPEELSAWRMHGPEGDFRLFLEIA
ADLHLETLGRAYDRFLCYGAYPGGGADGAPLYPSGLFAHGLEHPLDLEAIAEDHTYARMENRGLMHPPFMGSTSPDGADE
QGYSWCKAPRLSNEPCETGALARQVMDGQPLVRALVARAGGNVFSRVIARLLEVARVLIQMETWTKALEPGAPWCVPAGT
IGTGRAVGLTEAARGALGHWLRVENGRIAGYQIIAPTTWNFSPRDARGLPGPLERALIGAPVQSGETTPVSVQHIVRSFD
PCMACTVH

Sequences:

>Translated_488_residues
MSEAATRLIVGPFNRVEGDLEVRLEMEDGVVTAAFVNATLFRGFERILEGRDPSDALVIAPRICGICSVSQSHAAALALA
GVMGIAPARNGQIAANITLATENLADHLTHFHLFFMPDFARGPYRDRPWFDRAEARFAALRGASVRPMMQTRALLLHVMG
LIAGHWPHTLAIQPGGFSRGVDARDKIKLLAILSGVRAALEERLFGTALERVAALADPEELSAWRMHGPEGDFRLFLEIA
ADLHLETLGRAYDRFLCYGAYPGGGADGAPLYPSGLFAHGLEHPLDLEAIAEDHTYARMENRGLMHPPFMGSTSPDGADE
QGYSWCKAPRLSNEPCETGALARQVMDGQPLVRALVARAGGNVFSRVIARLLEVARVLIQMETWTKALEPGAPWCVPAGT
IGTGRAVGLTEAARGALGHWLRVENGRIAGYQIIAPTTWNFSPRDARGLPGPLERALIGAPVQSGETTPVSVQHIVRSFD
PCMACTVH
>Mature_487_residues
SEAATRLIVGPFNRVEGDLEVRLEMEDGVVTAAFVNATLFRGFERILEGRDPSDALVIAPRICGICSVSQSHAAALALAG
VMGIAPARNGQIAANITLATENLADHLTHFHLFFMPDFARGPYRDRPWFDRAEARFAALRGASVRPMMQTRALLLHVMGL
IAGHWPHTLAIQPGGFSRGVDARDKIKLLAILSGVRAALEERLFGTALERVAALADPEELSAWRMHGPEGDFRLFLEIAA
DLHLETLGRAYDRFLCYGAYPGGGADGAPLYPSGLFAHGLEHPLDLEAIAEDHTYARMENRGLMHPPFMGSTSPDGADEQ
GYSWCKAPRLSNEPCETGALARQVMDGQPLVRALVARAGGNVFSRVIARLLEVARVLIQMETWTKALEPGAPWCVPAGTI
GTGRAVGLTEAARGALGHWLRVENGRIAGYQIIAPTTWNFSPRDARGLPGPLERALIGAPVQSGETTPVSVQHIVRSFDP
CMACTVH

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=575, Percent_Identity=25.7391304347826, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787207, Length=591, Percent_Identity=27.7495769881557, Blast_Score=159, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 52736; Mature: 52605

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAATRLIVGPFNRVEGDLEVRLEMEDGVVTAAFVNATLFRGFERILEGRDPSDALVIA
CCCCCCEEEECCHHHCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEC
PRICGICSVSQSHAAALALAGVMGIAPARNGQIAANITLATENLADHLTHFHLFFMPDFA
CHHHCHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHHHHHHHHHEEEEECCCCC
RGPYRDRPWFDRAEARFAALRGASVRPMMQTRALLLHVMGLIAGHWPHTLAIQPGGFSRG
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
VDARDKIKLLAILSGVRAALEERLFGTALERVAALADPEELSAWRMHGPEGDFRLFLEIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEHHHHH
ADLHLETLGRAYDRFLCYGAYPGGGADGAPLYPSGLFAHGLEHPLDLEAIAEDHTYARME
HHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCHHHHHHH
NRGLMHPPFMGSTSPDGADEQGYSWCKAPRLSNEPCETGALARQVMDGQPLVRALVARAG
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCC
GNVFSRVIARLLEVARVLIQMETWTKALEPGAPWCVPAGTIGTGRAVGLTEAARGALGHW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCE
LRVENGRIAGYQIIAPTTWNFSPRDARGLPGPLERALIGAPVQSGETTPVSVQHIVRSFD
EEECCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
PCMACTVH
CCCEEECC
>Mature Secondary Structure 
SEAATRLIVGPFNRVEGDLEVRLEMEDGVVTAAFVNATLFRGFERILEGRDPSDALVIA
CCCCCEEEECCHHHCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEC
PRICGICSVSQSHAAALALAGVMGIAPARNGQIAANITLATENLADHLTHFHLFFMPDFA
CHHHCHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHHHHHHHHHEEEEECCCCC
RGPYRDRPWFDRAEARFAALRGASVRPMMQTRALLLHVMGLIAGHWPHTLAIQPGGFSRG
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
VDARDKIKLLAILSGVRAALEERLFGTALERVAALADPEELSAWRMHGPEGDFRLFLEIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEHHHHH
ADLHLETLGRAYDRFLCYGAYPGGGADGAPLYPSGLFAHGLEHPLDLEAIAEDHTYARME
HHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHCCHHHHHHH
NRGLMHPPFMGSTSPDGADEQGYSWCKAPRLSNEPCETGALARQVMDGQPLVRALVARAG
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCC
GNVFSRVIARLLEVARVLIQMETWTKALEPGAPWCVPAGTIGTGRAVGLTEAARGALGHW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCE
LRVENGRIAGYQIIAPTTWNFSPRDARGLPGPLERALIGAPVQSGETTPVSVQHIVRSFD
EEECCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
PCMACTVH
CCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]