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The map label for this gene is hyaA [C]

Identifier: 182678131

GI number: 182678131

Start: 1326787

End: 1327788

Strand: Direct

Name: hyaA [C]

Synonym: Bind_1146

Alternate gene names: 182678131

Gene position: 1326787-1327788 (Clockwise)

Preceding gene: 182678130

Following gene: 182678132

Centisome position: 31.82

GC content: 62.77

Gene sequence:

>1002_bases
ATGGTCTATAATGTGCTCTGGCTGCAATCGGGAGGCTGCGGCGGCTGCACCATGTCCATGCTCGCCGCCGAGGCGCCGGA
TCTCCTCACGACCTTGGAGGGGGCCGGCATTCGTTTTCTCTGGCATCCAAGTCTGTCCGAGGCCACCGGCGCCGAGGCCC
GTGCGATTTTCGACGATATATTGGCCGGACGCCTGCCGCTTGATGCTTTCTGCGTGGAAGGCGCTTTGTTGCGCGGACCG
AACGGCACGGGCAAATTTCACATTCTGGCCGGCACGGGCGAGCCGGCCGTGGATTGGGCAAGACGTCTGGCGGCCATTGC
CCGGCACGTGGTGGCGGTTGGGACTTGCGCGGCTTATGGCGGTGTGACGGCGGCTTCTCCCAATTTGCTCGATGCGTGTG
GTCTCGCTTATGAGGGCTTTTATCCGGGCGGTGCCTTGGGCGCCTCATTCCGTTCCCGCTCCGGCCTGCCGGTGGTCAAT
ATTGCGGGCTGTCCCACCCATCCGAATTGGGTGACGGAAACCTTGATGCTTCTGGCTGCTGAAAGCTTGTCCGAGGATGA
TCTCGATCCGCTGGGACGGCCGCGTTTTTATGCGGATCACCTCGTGCATCACGGCTGTACGCGCAATGAATATTACGAAT
ATAAGGCCAGCGCGCGCGAACCCTCGGACCTTGGTTGCATGATGGAACATATGGGCTGTATCGGCACGCAGGCCCATGCG
GATTGCAACACACGGGCGTGGAACGGCGAGGGGTCGTGCACACGCGGCGGCTATGCCTGTATCCATTGCACGGCGCCGGA
ATTCGAGGAGCCCGGCCATCTCTTTTTGGAAACGCCGAAGATCGCGGGCATTCCCGTGGGCCTGCCGACCGATATGCCGA
AGGCCTGGTTCGTCGCTTTGTCGTCGCTCGCCAAGGCCGCAACGCCGGAACGCCTCGCGCGCAATGCGACTAGCGATCAC
GTCCTGATCCCGCCCGTGACGCGCGTGCCGCGACGGAGCTAG

Upstream 100 bases:

>100_bases
GGTGTCACGGTCCAGTGAGGACAAGCGGATACGGTTTAAAAAGCGACGTATCGGGTATCTGTGATTCCCGATACTAGCCC
ATGGCGAGGGGATCGATCCA

Downstream 100 bases:

>100_bases
CGAGAGTATGAGCGAGGCAGCGACGCGCCTGATTGTGGGACCGTTCAACCGTGTCGAGGGTGATCTCGAAGTGCGGCTGG
AGATGGAAGATGGCGTTGTG

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MVYNVLWLQSGGCGGCTMSMLAAEAPDLLTTLEGAGIRFLWHPSLSEATGAEARAIFDDILAGRLPLDAFCVEGALLRGP
NGTGKFHILAGTGEPAVDWARRLAAIARHVVAVGTCAAYGGVTAASPNLLDACGLAYEGFYPGGALGASFRSRSGLPVVN
IAGCPTHPNWVTETLMLLAAESLSEDDLDPLGRPRFYADHLVHHGCTRNEYYEYKASAREPSDLGCMMEHMGCIGTQAHA
DCNTRAWNGEGSCTRGGYACIHCTAPEFEEPGHLFLETPKIAGIPVGLPTDMPKAWFVALSSLAKAATPERLARNATSDH
VLIPPVTRVPRRS

Sequences:

>Translated_333_residues
MVYNVLWLQSGGCGGCTMSMLAAEAPDLLTTLEGAGIRFLWHPSLSEATGAEARAIFDDILAGRLPLDAFCVEGALLRGP
NGTGKFHILAGTGEPAVDWARRLAAIARHVVAVGTCAAYGGVTAASPNLLDACGLAYEGFYPGGALGASFRSRSGLPVVN
IAGCPTHPNWVTETLMLLAAESLSEDDLDPLGRPRFYADHLVHHGCTRNEYYEYKASAREPSDLGCMMEHMGCIGTQAHA
DCNTRAWNGEGSCTRGGYACIHCTAPEFEEPGHLFLETPKIAGIPVGLPTDMPKAWFVALSSLAKAATPERLARNATSDH
VLIPPVTRVPRRS
>Mature_333_residues
MVYNVLWLQSGGCGGCTMSMLAAEAPDLLTTLEGAGIRFLWHPSLSEATGAEARAIFDDILAGRLPLDAFCVEGALLRGP
NGTGKFHILAGTGEPAVDWARRLAAIARHVVAVGTCAAYGGVTAASPNLLDACGLAYEGFYPGGALGASFRSRSGLPVVN
IAGCPTHPNWVTETLMLLAAESLSEDDLDPLGRPRFYADHLVHHGCTRNEYYEYKASAREPSDLGCMMEHMGCIGTQAHA
DCNTRAWNGEGSCTRGGYACIHCTAPEFEEPGHLFLETPKIAGIPVGLPTDMPKAWFVALSSLAKAATPERLARNATSDH
VLIPPVTRVPRRS

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=289, Percent_Identity=24.5674740484429, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789371, Length=245, Percent_Identity=29.7959183673469, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 35334; Mature: 35334

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00277 STAPH_STREP_TOXIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYNVLWLQSGGCGGCTMSMLAAEAPDLLTTLEGAGIRFLWHPSLSEATGAEARAIFDDI
CEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
LAGRLPLDAFCVEGALLRGPNGTGKFHILAGTGEPAVDWARRLAAIARHVVAVGTCAAYG
HHCCCCHHHHHHCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GVTAASPNLLDACGLAYEGFYPGGALGASFRSRSGLPVVNIAGCPTHPNWVTETLMLLAA
CCCCCCCCHHHHHCCHHCCCCCCCHHCCCHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
ESLSEDDLDPLGRPRFYADHLVHHGCTRNEYYEYKASAREPSDLGCMMEHMGCIGTQAHA
HHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCC
DCNTRAWNGEGSCTRGGYACIHCTAPEFEEPGHLFLETPKIAGIPVGLPTDMPKAWFVAL
CCCCEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHH
SSLAKAATPERLARNATSDHVLIPPVTRVPRRS
HHHHHHCCHHHHHHCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MVYNVLWLQSGGCGGCTMSMLAAEAPDLLTTLEGAGIRFLWHPSLSEATGAEARAIFDDI
CEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHH
LAGRLPLDAFCVEGALLRGPNGTGKFHILAGTGEPAVDWARRLAAIARHVVAVGTCAAYG
HHCCCCHHHHHHCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GVTAASPNLLDACGLAYEGFYPGGALGASFRSRSGLPVVNIAGCPTHPNWVTETLMLLAA
CCCCCCCCHHHHHCCHHCCCCCCCHHCCCHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
ESLSEDDLDPLGRPRFYADHLVHHGCTRNEYYEYKASAREPSDLGCMMEHMGCIGTQAHA
HHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCC
DCNTRAWNGEGSCTRGGYACIHCTAPEFEEPGHLFLETPKIAGIPVGLPTDMPKAWFVAL
CCCCEEECCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHH
SSLAKAATPERLARNATSDHVLIPPVTRVPRRS
HHHHHHCCHHHHHHCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]