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The map label for this gene is rph

Identifier: 182677771

GI number: 182677771

Start: 872009

End: 872722

Strand: Direct

Name: rph

Synonym: Bind_0778

Alternate gene names: 182677771

Gene position: 872009-872722 (Clockwise)

Preceding gene: 182677768

Following gene: 182677772

Centisome position: 20.91

GC content: 63.03

Gene sequence:

>714_bases
ATGCGTCCCTCCAAACGTGCCGCCCAGGACTTGCGTCCGGTGACGCTCGAACGCAATGTCGCCCGCTATGCCGAAGGCTC
CTGCCTCGTCAAATTTGGCGGCACTCATGTCCTTTGCACGGCCTCGCTCGAGGATAAGCCGCCGCCGTGGCTGCGCGGGC
AGGGACGTGGCTGGGTGACGGCTGAATATGCCATGTTGCCCCGCGCGACTCATACACGCACGCGCCGTGAATCCATGTCC
GGCAAGCCATCCGGGCGGACGCAGGAAATCCAGCGCCTGATCGGGCGCAGCCTGCGCGCGGTCACGAATCTTCCGGCTTT
AGGCGAGCGTCAGATCACCCTCGACTGCGATGTCTTGCAAGCCGATGGCGGCACGCGGACGGCCGCGATCACCGGGGCCT
GGGTTGCCTTGCATGATTGCTGCAAATGGATGCATGGCCGCTCGATCATCAAGGAATTTCCGTTGCGCGAACATGTGGCG
GCGGTGTCCTGCGGCATTTACCAGGGCGAAGCGGTTCTCGATCTTGATTACGAGGAGGATTCTGTCGCCCAGACCGATGC
AAATTTCGTCATGACGGGCGGCGGCGCTCTGGTCGAGGTGCAGGCGAGCGCCGAGGGGGCCGTCTTCAGCGAGGACGAGT
TGAGCGTCCTTTTGGCTTTGGCCAAGGGCGGGATCGCCCAATTGGTGAATATCCAGAAGCTCGCGATCGGCTGA

Upstream 100 bases:

>100_bases
TTTACGGGTTTCACTCGCGCTGAAACCGCGCTAAAGGCGAGCGCCGCGAGGTTGAAGATCGGATGTCCTCTGATCAGTCT
CACCCCATTGAGGAAGATTC

Downstream 100 bases:

>100_bases
CCCCATGCCTTTATCCCCTTCCGGTGCCGACCGATTGGTGATCGCCACGCATAATGCCGGCAAGCTGCGGGAAATGCGCG
AATTGCTCGCGCCCTATCGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPSKRAAQDLRPVTLERNVARYAEGSCLVKFGGTHVLCTASLEDKPPPWLRGQGRGWVTAEYAMLPRATHTRTRRESMS
GKPSGRTQEIQRLIGRSLRAVTNLPALGERQITLDCDVLQADGGTRTAAITGAWVALHDCCKWMHGRSIIKEFPLREHVA
AVSCGIYQGEAVLDLDYEEDSVAQTDANFVMTGGGALVEVQASAEGAVFSEDELSVLLALAKGGIAQLVNIQKLAIG

Sequences:

>Translated_237_residues
MRPSKRAAQDLRPVTLERNVARYAEGSCLVKFGGTHVLCTASLEDKPPPWLRGQGRGWVTAEYAMLPRATHTRTRRESMS
GKPSGRTQEIQRLIGRSLRAVTNLPALGERQITLDCDVLQADGGTRTAAITGAWVALHDCCKWMHGRSIIKEFPLREHVA
AVSCGIYQGEAVLDLDYEEDSVAQTDANFVMTGGGALVEVQASAEGAVFSEDELSVLLALAKGGIAQLVNIQKLAIG
>Mature_237_residues
MRPSKRAAQDLRPVTLERNVARYAEGSCLVKFGGTHVLCTASLEDKPPPWLRGQGRGWVTAEYAMLPRATHTRTRRESMS
GKPSGRTQEIQRLIGRSLRAVTNLPALGERQITLDCDVLQADGGTRTAAITGAWVALHDCCKWMHGRSIIKEFPLREHVA
AVSCGIYQGEAVLDLDYEEDSVAQTDANFVMTGGGALVEVQASAEGAVFSEDELSVLLALAKGGIAQLVNIQKLAIG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=60.4761904761905, Blast_Score=254, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_BEII9 (B2IH14)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001831917.1
- GeneID:   6200287
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_0778
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   CLSK2405305
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25613; Mature: 25613

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSKRAAQDLRPVTLERNVARYAEGSCLVKFGGTHVLCTASLEDKPPPWLRGQGRGWVT
CCCCHHHHHHCCCEEHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEE
AEYAMLPRATHTRTRRESMSGKPSGRTQEIQRLIGRSLRAVTNLPALGERQITLDCDVLQ
EEHEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEE
ADGGTRTAAITGAWVALHDCCKWMHGRSIIKEFPLREHVAAVSCGIYQGEAVLDLDYEED
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCEEEEECCCCC
SVAQTDANFVMTGGGALVEVQASAEGAVFSEDELSVLLALAKGGIAQLVNIQKLAIG
CCCCCCCCEEEECCCEEEEEEECCCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSKRAAQDLRPVTLERNVARYAEGSCLVKFGGTHVLCTASLEDKPPPWLRGQGRGWVT
CCCCHHHHHHCCCEEHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEE
AEYAMLPRATHTRTRRESMSGKPSGRTQEIQRLIGRSLRAVTNLPALGERQITLDCDVLQ
EEHEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEE
ADGGTRTAAITGAWVALHDCCKWMHGRSIIKEFPLREHVAAVSCGIYQGEAVLDLDYEED
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEECCCEEEEECCCCC
SVAQTDANFVMTGGGALVEVQASAEGAVFSEDELSVLLALAKGGIAQLVNIQKLAIG
CCCCCCCCEEEECCCEEEEEEECCCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA