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The map label for this gene is cspA [C]

Identifier: 182677655

GI number: 182677655

Start: 744514

End: 744726

Strand: Direct

Name: cspA [C]

Synonym: Bind_0662

Alternate gene names: 182677655

Gene position: 744514-744726 (Clockwise)

Preceding gene: 182677654

Following gene: 182677658

Centisome position: 17.85

GC content: 53.05

Gene sequence:

>213_bases
ATGTCGCAGCAGGGAACGGTTAAATTCTTCAATTCAGACAAGGGTTATGGTTTCATCAAGCCGGATGATGGCGGGCGCGA
TATTTTCGTGCATATCACCGCGGTCGAGCGCGCCGGTCTTCAGGCGCTGAATGAAGGCCAGCGTCTTTCCTATGAGATTG
AACCGGACAAGAAAGGCAAAGGCCCCAAGGCCGTCGATCTGAAGGTGGATTAG

Upstream 100 bases:

>100_bases
TCCGGGCTTGGACCACGCAAGGGGCAGGGTCTGATCTGCCTGGACTCACCGGCTGGCGTCGCATCGAGCGATCGCGGCTG
GTCAGGGGAGGAAGGTGTCC

Downstream 100 bases:

>100_bases
TCGCGGGCGGAATCGACAAGGCCTTGATTGGTGATCACAACCCAGCCTTGTCCCCTTTTTTCGATAGACTGGACACGGCC
GGCGCCGGGAATCATCTCAC

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MSQQGTVKFFNSDKGYGFIKPDDGGRDIFVHITAVERAGLQALNEGQRLSYEIEPDKKGKGPKAVDLKVD

Sequences:

>Translated_70_residues
MSQQGTVKFFNSDKGYGFIKPDDGGRDIFVHITAVERAGLQALNEGQRLSYEIEPDKKGKGPKAVDLKVD
>Mature_69_residues
SQQGTVKFFNSDKGYGFIKPDDGGRDIFVHITAVERAGLQALNEGQRLSYEIEPDKKGKGPKAVDLKVD

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=55, Percent_Identity=54.5454545454545, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI2367114, Length=63, Percent_Identity=47.6190476190476, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=46.875, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=46.875, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=43.2835820895522, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787834, Length=63, Percent_Identity=44.4444444444444, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7712; Mature: 7581

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQGTVKFFNSDKGYGFIKPDDGGRDIFVHITAVERAGLQALNEGQRLSYEIEPDKKGK
CCCCCEEEEEECCCCEEEECCCCCCCEEEEEEEEECCCCCHHCCCCCEEEEEECCCCCCC
GPKAVDLKVD
CCCEEEEEEC
>Mature Secondary Structure 
SQQGTVKFFNSDKGYGFIKPDDGGRDIFVHITAVERAGLQALNEGQRLSYEIEPDKKGK
CCCCEEEEEECCCCEEEECCCCCCCEEEEEEEEECCCCCHHCCCCCEEEEEECCCCCCC
GPKAVDLKVD
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]