| Definition | Ralstonia solanacearum GMI1000, complete genome. |
|---|---|
| Accession | NC_003295 |
| Length | 3,716,413 |
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The map label for this gene is livF [H]
Identifier: 17548060
GI number: 17548060
Start: 3603651
End: 3604391
Strand: Reverse
Name: livF [H]
Synonym: RSc3343
Alternate gene names: 17548060
Gene position: 3604391-3603651 (Counterclockwise)
Preceding gene: 17548061
Following gene: 17548059
Centisome position: 96.99
GC content: 68.29
Gene sequence:
>741_bases ATGAGCACGCCCGCGCTCCAGATCAAGGACCTGCACGCCTGGTACGGCGAGTCGCACATCCTGCACGGCGTCGACCTGAC CGTGAACCGCGGCGAGGTCGTCACGCTGCTCGGCCGCAACGGCGCGGGCCGCACCACCACGCTGCGCGCCATCATGGGCC TGGTCGGCACGCGCAAGGGGTCCATCGTCGTGCACGACGATGCCGGCCAGGGTGTCGAGACCATCGGCCTGCCCACGCAC CGCATCGCCCATTGCGGCATCGGCTATTGCCCGGAGGAGCGCGGCATCTTCGCCAGCCTGTCGTGCGAGGAGAACCTGCT GCTGCCGCCGGTGCTCAAGGGCCAGGTGGCCACGCGCGGCAACGCGGGCATGAGCGTGGCCGAGATCTACGAGATGTTTC CCAACCTGAAGGAGCGCCGCAACAGCCAGGGCACGCGCCTGTCGGGCGGCGAGCAGCAGATGCTGGCGGTCGGGCGCATC CTGCGTACGGGGGCCAACCTGCTGCTGCTCGACGAGATCTCCGAGGGCCTGGCGCCGGTGATCGTGCAGGCGCTGGCCCG CATGATCCTGATGCTCAAGAAGCGCGGCTACACGGTGGTGATGGTGGAGCAGAACTTCCGCTTCGCGGCGCCGCTGGCCG ACCGCTTCTACGTGATGGAACACGGTCGCATCGTCGAGCGTTTCGAGGCGGCCCAGCTGCAGGACAAGATGCCGATACTG CACGAGCTGCTCGGCGTTTGA
Upstream 100 bases:
>100_bases AAGGCCACTGACGCCGCCCGCGGCCATCGGCGCGGCGGGGGGCACGGCACGCCACCGCATGCGTCATCGAATGTTTGACC CGGACCCACGAGAACACCGC
Downstream 100 bases:
>100_bases TCGCTTTGCTTGACCTTTGCGCGGGGCGTGATCCGTCCGGCGCGCACGACACAGGAGACAAGACGATGACACTCAAGAAG CTGGCTGGTGCACTGATGGC
Product: ABC transporter ATP-binding protein
Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]
Alternate protein names: LIV-I protein F [H]
Number of amino acids: Translated: 246; Mature: 245
Protein sequence:
>246_residues MSTPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKGSIVVHDDAGQGVETIGLPTH RIAHCGIGYCPEERGIFASLSCEENLLLPPVLKGQVATRGNAGMSVAEIYEMFPNLKERRNSQGTRLSGGEQQMLAVGRI LRTGANLLLLDEISEGLAPVIVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPIL HELLGV
Sequences:
>Translated_246_residues MSTPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKGSIVVHDDAGQGVETIGLPTH RIAHCGIGYCPEERGIFASLSCEENLLLPPVLKGQVATRGNAGMSVAEIYEMFPNLKERRNSQGTRLSGGEQQMLAVGRI LRTGANLLLLDEISEGLAPVIVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPIL HELLGV >Mature_245_residues STPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKGSIVVHDDAGQGVETIGLPTHR IAHCGIGYCPEERGIFASLSCEENLLLPPVLKGQVATRGNAGMSVAEIYEMFPNLKERRNSQGTRLSGGEQQMLAVGRIL RTGANLLLLDEISEGLAPVIVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPILH ELLGV
Specific function: Component of the leucine-specific transport system [H]
COG id: COG0410
COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ABC transporter domain [H]
Homologues:
Organism=Homo sapiens, GI31657092, Length=224, Percent_Identity=27.2321428571429, Blast_Score=84, Evalue=9e-17, Organism=Homo sapiens, GI105990541, Length=201, Percent_Identity=27.8606965174129, Blast_Score=81, Evalue=1e-15, Organism=Homo sapiens, GI21536376, Length=205, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=8e-15, Organism=Homo sapiens, GI45446740, Length=206, Percent_Identity=28.1553398058252, Blast_Score=76, Evalue=3e-14, Organism=Homo sapiens, GI47078218, Length=206, Percent_Identity=28.1553398058252, Blast_Score=76, Evalue=3e-14, Organism=Homo sapiens, GI27881501, Length=208, Percent_Identity=29.3269230769231, Blast_Score=75, Evalue=5e-14, Organism=Homo sapiens, GI30795238, Length=208, Percent_Identity=29.3269230769231, Blast_Score=75, Evalue=5e-14, Organism=Homo sapiens, GI27436953, Length=202, Percent_Identity=27.7227722772277, Blast_Score=75, Evalue=6e-14, Organism=Homo sapiens, GI116734710, Length=201, Percent_Identity=27.8606965174129, Blast_Score=74, Evalue=2e-13, Organism=Homo sapiens, GI150417984, Length=203, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12, Organism=Homo sapiens, GI153792144, Length=205, Percent_Identity=25.8536585365854, Blast_Score=68, Evalue=6e-12, Organism=Homo sapiens, GI42741659, Length=248, Percent_Identity=27.0161290322581, Blast_Score=68, Evalue=7e-12, Organism=Homo sapiens, GI27477115, Length=203, Percent_Identity=25.1231527093596, Blast_Score=65, Evalue=6e-11, Organism=Homo sapiens, GI4505771, Length=255, Percent_Identity=25.8823529411765, Blast_Score=65, Evalue=8e-11, Organism=Homo sapiens, GI9961250, Length=255, Percent_Identity=25.8823529411765, Blast_Score=64, Evalue=9e-11, Organism=Homo sapiens, GI9961252, Length=255, Percent_Identity=25.8823529411765, Blast_Score=64, Evalue=9e-11, Organism=Escherichia coli, GI87082268, Length=225, Percent_Identity=40.8888888888889, Blast_Score=156, Evalue=1e-39, Organism=Escherichia coli, GI1789593, Length=224, Percent_Identity=32.5892857142857, Blast_Score=108, Evalue=3e-25, Organism=Escherichia coli, GI1788225, Length=234, Percent_Identity=31.1965811965812, Blast_Score=98, Evalue=4e-22, Organism=Escherichia coli, GI1789672, Length=223, Percent_Identity=27.3542600896861, Blast_Score=96, Evalue=2e-21, Organism=Escherichia coli, GI1787029, Length=221, Percent_Identity=28.5067873303167, Blast_Score=93, Evalue=2e-20, Organism=Escherichia coli, GI1789864, Length=263, Percent_Identity=26.2357414448669, Blast_Score=88, Evalue=6e-19, Organism=Escherichia coli, GI1788897, Length=221, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=7e-18, Organism=Escherichia coli, GI1790525, Length=235, Percent_Identity=26.8085106382979, Blast_Score=82, Evalue=3e-17, Organism=Escherichia coli, GI1787089, Length=227, Percent_Identity=26.431718061674, Blast_Score=80, Evalue=9e-17, Organism=Escherichia coli, GI87081791, Length=229, Percent_Identity=27.9475982532751, Blast_Score=79, Evalue=2e-16, Organism=Escherichia coli, GI1788450, Length=217, Percent_Identity=29.9539170506912, Blast_Score=77, Evalue=1e-15, Organism=Escherichia coli, GI1788644, Length=248, Percent_Identity=29.8387096774194, Blast_Score=76, Evalue=3e-15, Organism=Escherichia coli, GI1787792, Length=223, Percent_Identity=29.1479820627803, Blast_Score=74, Evalue=7e-15, Organism=Escherichia coli, GI48995001, Length=235, Percent_Identity=25.9574468085106, Blast_Score=74, Evalue=8e-15, Organism=Escherichia coli, GI1787105, Length=230, Percent_Identity=27.8260869565217, Blast_Score=74, Evalue=1e-14, Organism=Escherichia coli, GI1787370, Length=225, Percent_Identity=27.5555555555556, Blast_Score=74, Evalue=1e-14, Organism=Escherichia coli, GI1786703, Length=222, Percent_Identity=28.8288288288288, Blast_Score=73, Evalue=2e-14, Organism=Escherichia coli, GI1790467, Length=223, Percent_Identity=26.9058295964126, Blast_Score=72, Evalue=2e-14, Organism=Escherichia coli, GI1790190, Length=232, Percent_Identity=25, Blast_Score=72, Evalue=4e-14, Organism=Escherichia coli, GI1788761, Length=228, Percent_Identity=27.6315789473684, Blast_Score=72, Evalue=4e-14, Organism=Escherichia coli, GI1786872, Length=223, Percent_Identity=28.6995515695067, Blast_Score=72, Evalue=4e-14, Organism=Escherichia coli, GI1787112, Length=243, Percent_Identity=28.8065843621399, Blast_Score=70, Evalue=1e-13, Organism=Escherichia coli, GI1786253, Length=222, Percent_Identity=30.1801801801802, Blast_Score=70, Evalue=1e-13, Organism=Escherichia coli, GI48994943, Length=236, Percent_Identity=24.1525423728814, Blast_Score=69, Evalue=2e-13, Organism=Escherichia coli, GI87081782, Length=244, Percent_Identity=25.8196721311475, Blast_Score=69, Evalue=3e-13, Organism=Escherichia coli, GI1788210, Length=249, Percent_Identity=24.4979919678715, Blast_Score=68, Evalue=6e-13, Organism=Escherichia coli, GI1786398, Length=227, Percent_Identity=23.3480176211454, Blast_Score=68, Evalue=6e-13, Organism=Escherichia coli, GI1789991, Length=231, Percent_Identity=29.4372294372294, Blast_Score=68, Evalue=6e-13, Organism=Escherichia coli, GI1786319, Length=225, Percent_Identity=27.1111111111111, Blast_Score=67, Evalue=1e-12, Organism=Escherichia coli, GI87081709, Length=227, Percent_Identity=25.9911894273128, Blast_Score=65, Evalue=4e-12, Organism=Escherichia coli, GI1787712, Length=226, Percent_Identity=26.9911504424779, Blast_Score=65, Evalue=5e-12, Organism=Escherichia coli, GI87082267, Length=215, Percent_Identity=28.3720930232558, Blast_Score=63, Evalue=2e-11, Organism=Escherichia coli, GI1788165, Length=225, Percent_Identity=26.2222222222222, Blast_Score=62, Evalue=3e-11, Organism=Escherichia coli, GI1787759, Length=246, Percent_Identity=26.8292682926829, Blast_Score=62, Evalue=3e-11, Organism=Escherichia coli, GI1786563, Length=232, Percent_Identity=27.1551724137931, Blast_Score=62, Evalue=5e-11, Organism=Caenorhabditis elegans, GI115533608, Length=221, Percent_Identity=30.7692307692308, Blast_Score=83, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17510237, Length=206, Percent_Identity=30.5825242718447, Blast_Score=78, Evalue=5e-15, Organism=Caenorhabditis elegans, GI71983510, Length=207, Percent_Identity=27.536231884058, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI71983516, Length=207, Percent_Identity=27.536231884058, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI71983523, Length=207, Percent_Identity=27.536231884058, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI25146777, Length=222, Percent_Identity=27.4774774774775, Blast_Score=70, Evalue=9e-13, Organism=Caenorhabditis elegans, GI17565586, Length=219, Percent_Identity=26.4840182648402, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI24666092, Length=221, Percent_Identity=28.9592760180996, Blast_Score=90, Evalue=2e-18, Organism=Drosophila melanogaster, GI24648314, Length=220, Percent_Identity=26.3636363636364, Blast_Score=84, Evalue=8e-17, Organism=Drosophila melanogaster, GI116007328, Length=208, Percent_Identity=28.8461538461538, Blast_Score=79, Evalue=3e-15, Organism=Drosophila melanogaster, GI28574150, Length=208, Percent_Identity=28.8461538461538, Blast_Score=79, Evalue=3e-15, Organism=Drosophila melanogaster, GI221512771, Length=220, Percent_Identity=27.7272727272727, Blast_Score=77, Evalue=1e-14, Organism=Drosophila melanogaster, GI24643674, Length=220, Percent_Identity=26.3636363636364, Blast_Score=76, Evalue=2e-14, Organism=Drosophila melanogaster, GI85725262, Length=210, Percent_Identity=27.6190476190476, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI24650853, Length=232, Percent_Identity=25.8620689655172, Blast_Score=64, Evalue=1e-10,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003439 - InterPro: IPR017871 - InterPro: IPR003593 [H]
Pfam domain/function: PF00005 ABC_tran [H]
EC number: NA
Molecular weight: Translated: 26933; Mature: 26802
Theoretical pI: Translated: 8.12; Mature: 8.12
Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 4.1 %Met (Translated Protein) 5.3 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 3.7 %Met (Mature Protein) 4.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSTPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKG CCCCCEEEEHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCC SIVVHDDAGQGVETIGLPTHRIAHCGIGYCPEERGIFASLSCEENLLLPPVLKGQVATRG CEEEECCCCCCCEEECCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEECCC NAGMSVAEIYEMFPNLKERRNSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPV CCCCHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHH IVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPIL HHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHEEEECCCHHHHHHHHHHHHHCCHHH HELLGV HHHHCC >Mature Secondary Structure STPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKG CCCCEEEEHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCC SIVVHDDAGQGVETIGLPTHRIAHCGIGYCPEERGIFASLSCEENLLLPPVLKGQVATRG CEEEECCCCCCCEEECCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEECCC NAGMSVAEIYEMFPNLKERRNSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPV CCCCHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHH IVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPIL HHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHEEEECCCHHHHHHHHHHHHHCCHHH HELLGV HHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]
Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 2195019; 8041620; 9278503 [H]