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The map label for this gene is livK [C]

Identifier: 17548017

GI number: 17548017

Start: 3557507

End: 3558640

Strand: Reverse

Name: livK [C]

Synonym: RSc3300

Alternate gene names: 17548017

Gene position: 3558640-3557507 (Counterclockwise)

Preceding gene: 17548018

Following gene: 17548016

Centisome position: 95.75

GC content: 65.34

Gene sequence:

>1134_bases
ATGAAATTGAAGCAACTGTGGGTGGCGGCGGCACTGTGTGCGCTGGGCAGCGCGGCATCGGCTCAGGAAGTCATCAAGCT
GGGTTTCGCCGCGCCGATGACCGGCGCCCAGGCCCAGTACGGCAAGGACATGCAGAACGGCGTGACCCTGGCCATCGAAG
AGTTCAACGCCACCAAGCCGAAGATCGCCGGCAAGGATGTGAAATTCCAGCTGGTGTCGGAAGACGACCAGGCCGATCCG
AAGACCGGCACCACCGTCGCGCAGAAGCTGGTGGACGGTGGCATCAAGGGCATGGTCGGCCACTTCAACTCGGGCACCAG
CATCCCGGCCTCGCGTGTGTACAACCAGGCCGGCATCGCGCAGATCGCCATGGCGACCGCCCCGGAATACACGAAGCAGG
GCTACAAGACCACCTTCCGCATGATGACCTCCGACACCCAGCAGGGTTCGGTGGTCGGCACCTTCGCCGTCAAGAAGCTG
GGCTACAAGAACCTCGCCATCGTCGATGACCGCACGGCCTATGGCCAGGGCCTGGCCGACGAGTTCGAGAAGGCCGCCAA
GGCCGCCGGCGCCACCATCGTGCGTCGGGAGTACACCAACGACAAGGCCAGCGACTTCAAGGCGATCCTCACGCAGATCA
AGAGCAAGAACCCGGACGCCGTGTTCTACGGCGGCGCCGAAGGCCAGTCGGCTCCGCTGGTCAAGCAGATGCGCGAGCTG
GGCATGAAGGCGACGCTGATGTCGGGCGAAATGTCCAAGACCGACGACTTCATCAAGCTGGCCGGCGCACCGGCCGCCGA
AGGCGTGGTGTGCTCGCTGGCCGGCCTGCCGCTGGAGCAGATGCCGGGCGGCGCGGGCTACAAGCAGCGCTACGAGAAGC
GTTTCGGCTCGACGGTGCAGACGTACTCGCCGTACGCCTACGACGGCGCGATGGCCCTGATGAAGGCGATGGTCGCCGCC
GGTTCGTCCGACCCGAGCAAGTACCTGCCGATCCTGGCCGCCACCAACGCGCAGGGCGTCACCGCCAAGCACTACGCCTA
CGACGACAAGGGCGACCTCAAGGACGGCGGCATCACCGTCTACAAGGTCACTGGCGGCAAGTGGGTGCCGCTCGAAAGCG
TGGGCGGCAAGTAA

Upstream 100 bases:

>100_bases
GCCTCCATGACAGGTGGGGGCGGGGCGCGAAGCGGGAGGAGGGCGGCGGCGATCCCGTCGCGCCATGGTTCCAAATTGCG
TTTGAATGGGAGATGCACAA

Downstream 100 bases:

>100_bases
GCCGGCCGCGTAGTCCGCAGCATGGAAGACCGCCCTCCGGGGCGGTTTTTCTTTGGCCGCGCGCATCGCGGCAGCCCGCC
GCCACCGAGGCGGCCGGGTT

Product: amino-acid transport signal peptide protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKLKQLWVAAALCALGSAASAQEVIKLGFAAPMTGAQAQYGKDMQNGVTLAIEEFNATKPKIAGKDVKFQLVSEDDQADP
KTGTTVAQKLVDGGIKGMVGHFNSGTSIPASRVYNQAGIAQIAMATAPEYTKQGYKTTFRMMTSDTQQGSVVGTFAVKKL
GYKNLAIVDDRTAYGQGLADEFEKAAKAAGATIVRREYTNDKASDFKAILTQIKSKNPDAVFYGGAEGQSAPLVKQMREL
GMKATLMSGEMSKTDDFIKLAGAPAAEGVVCSLAGLPLEQMPGGAGYKQRYEKRFGSTVQTYSPYAYDGAMALMKAMVAA
GSSDPSKYLPILAATNAQGVTAKHYAYDDKGDLKDGGITVYKVTGGKWVPLESVGGK

Sequences:

>Translated_377_residues
MKLKQLWVAAALCALGSAASAQEVIKLGFAAPMTGAQAQYGKDMQNGVTLAIEEFNATKPKIAGKDVKFQLVSEDDQADP
KTGTTVAQKLVDGGIKGMVGHFNSGTSIPASRVYNQAGIAQIAMATAPEYTKQGYKTTFRMMTSDTQQGSVVGTFAVKKL
GYKNLAIVDDRTAYGQGLADEFEKAAKAAGATIVRREYTNDKASDFKAILTQIKSKNPDAVFYGGAEGQSAPLVKQMREL
GMKATLMSGEMSKTDDFIKLAGAPAAEGVVCSLAGLPLEQMPGGAGYKQRYEKRFGSTVQTYSPYAYDGAMALMKAMVAA
GSSDPSKYLPILAATNAQGVTAKHYAYDDKGDLKDGGITVYKVTGGKWVPLESVGGK
>Mature_377_residues
MKLKQLWVAAALCALGSAASAQEVIKLGFAAPMTGAQAQYGKDMQNGVTLAIEEFNATKPKIAGKDVKFQLVSEDDQADP
KTGTTVAQKLVDGGIKGMVGHFNSGTSIPASRVYNQAGIAQIAMATAPEYTKQGYKTTFRMMTSDTQQGSVVGTFAVKKL
GYKNLAIVDDRTAYGQGLADEFEKAAKAAGATIVRREYTNDKASDFKAILTQIKSKNPDAVFYGGAEGQSAPLVKQMREL
GMKATLMSGEMSKTDDFIKLAGAPAAEGVVCSLAGLPLEQMPGGAGYKQRYEKRFGSTVQTYSPYAYDGAMALMKAMVAA
GSSDPSKYLPILAATNAQGVTAKHYAYDDKGDLKDGGITVYKVTGGKWVPLESVGGK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=369, Percent_Identity=31.1653116531165, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI48994941, Length=357, Percent_Identity=31.0924369747899, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39796; Mature: 39796

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKQLWVAAALCALGSAASAQEVIKLGFAAPMTGAQAQYGKDMQNGVTLAIEEFNATKP
CCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCCHHHCCEEEEEECCCCCCC
KIAGKDVKFQLVSEDDQADPKTGTTVAQKLVDGGIKGMVGHFNSGTSIPASRVYNQAGIA
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHEHEECCCCCCCCHHHHHHHHCHH
QIAMATAPEYTKQGYKTTFRMMTSDTQQGSVVGTFAVKKLGYKNLAIVDDRTAYGQGLAD
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHCCCCCEEEEECCHHHCCCHHH
EFEKAAKAAGATIVRREYTNDKASDFKAILTQIKSKNPDAVFYGGAEGQSAPLVKQMREL
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHC
GMKATLMSGEMSKTDDFIKLAGAPAAEGVVCSLAGLPLEQMPGGAGYKQRYEKRFGSTVQ
CCEEEEECCCCCCCCCHHEECCCCCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHCCCEE
TYSPYAYDGAMALMKAMVAAGSSDPSKYLPILAATNAQGVTAKHYAYDDKGDLKDGGITV
CCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCEEE
YKVTGGKWVPLESVGGK
EEECCCEEEEHHCCCCC
>Mature Secondary Structure
MKLKQLWVAAALCALGSAASAQEVIKLGFAAPMTGAQAQYGKDMQNGVTLAIEEFNATKP
CCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHCCHHHCCEEEEEECCCCCCC
KIAGKDVKFQLVSEDDQADPKTGTTVAQKLVDGGIKGMVGHFNSGTSIPASRVYNQAGIA
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHEHEECCCCCCCCHHHHHHHHCHH
QIAMATAPEYTKQGYKTTFRMMTSDTQQGSVVGTFAVKKLGYKNLAIVDDRTAYGQGLAD
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHCCCCCEEEEECCHHHCCCHHH
EFEKAAKAAGATIVRREYTNDKASDFKAILTQIKSKNPDAVFYGGAEGQSAPLVKQMREL
HHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHC
GMKATLMSGEMSKTDDFIKLAGAPAAEGVVCSLAGLPLEQMPGGAGYKQRYEKRFGSTVQ
CCEEEEECCCCCCCCCHHEECCCCCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHCCCEE
TYSPYAYDGAMALMKAMVAAGSSDPSKYLPILAATNAQGVTAKHYAYDDKGDLKDGGITV
CCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCEEE
YKVTGGKWVPLESVGGK
EEECCCEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA