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The map label for this gene is gcvH

Identifier: 17548011

GI number: 17548011

Start: 3547113

End: 3547496

Strand: Direct

Name: gcvH

Synonym: RSc3294

Alternate gene names: 17548011

Gene position: 3547113-3547496 (Clockwise)

Preceding gene: 17548010

Following gene: 17548012

Centisome position: 95.44

GC content: 65.1

Gene sequence:

>384_bases
ATGAGCAACACGCCCGTAGAGCTGAAGTACACCGAATCCCACGAGTGGGTCCGCACGGAGGCCGACGGCACGCTGACCAT
CGGCATCACCGACCTGGCGCAGGAGCAGCTCGGCGACATCGTCTTCCTGGAGCTGCCGGACACCGGCCGCCAGGTCAAGC
AGGGTGAAGCGATTGCCGTGGTGGAGTCGGTCAAGGCCGCCTCCGACATCTACGCGCCGGTGTCGGGCGAAGTGATCGCC
AGCAACGCCGATGCCACCGAGTCGCCCGAGTCGGTCAATGAAGACGCCTATGACGCCTGGCTGTTCAAGATCAAGCCGGC
CAACGCCGACGACGTGAACGCCCTGCTGTCGGCCGACCAGTACCAAGCCAAAGCCGGGGCGTAA

Upstream 100 bases:

>100_bases
GCGACTGTGGTTAAACTGCCGTTCGTGCGCAATGGCAAGGCGCTGGTCAGCTGAATCCCGAATTCGAACCCATCCTCATC
CTTCTTCCGGAGAACCCTTC

Downstream 100 bases:

>100_bases
GCCTCGCCCCGCACCGGCGCGCCCGACCCGCGCGCCGGCCTGATCGTTTTACGAGCCCTTGCCATGAACGCTCCGCACCC
CGCTTCCGCGGCCCTGACCC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSNTPVELKYTESHEWVRTEADGTLTIGITDLAQEQLGDIVFLELPDTGRQVKQGEAIAVVESVKAASDIYAPVSGEVIA
SNADATESPESVNEDAYDAWLFKIKPANADDVNALLSADQYQAKAGA

Sequences:

>Translated_127_residues
MSNTPVELKYTESHEWVRTEADGTLTIGITDLAQEQLGDIVFLELPDTGRQVKQGEAIAVVESVKAASDIYAPVSGEVIA
SNADATESPESVNEDAYDAWLFKIKPANADDVNALLSADQYQAKAGA
>Mature_126_residues
SNTPVELKYTESHEWVRTEADGTLTIGITDLAQEQLGDIVFLELPDTGRQVKQGEAIAVVESVKAASDIYAPVSGEVIAS
NADATESPESVNEDAYDAWLFKIKPANADDVNALLSADQYQAKAGA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=115, Percent_Identity=43.4782608695652, Blast_Score=104, Evalue=1e-23,
Organism=Homo sapiens, GI89057342, Length=115, Percent_Identity=42.6086956521739, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=56.4516129032258, Blast_Score=143, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=49.5575221238938, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=43.3628318584071, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=41.6, Blast_Score=102, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=44.9152542372881, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_RALSO (Q8XU99)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_521413.1
- ProteinModelPortal:   Q8XU99
- SMR:   Q8XU99
- GeneID:   1222158
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc3294
- NMPDR:   fig|267608.1.peg.3292
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   RSOL267608:RSC3294-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13571; Mature: 13440

Theoretical pI: Translated: 3.84; Mature: 3.84

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTPVELKYTESHEWVRTEADGTLTIGITDLAQEQLGDIVFLELPDTGRQVKQGEAIAV
CCCCCEEEEECCCCCEEEECCCCEEEEEEHHHHHHHHCCEEEEECCCCCCCCCCCCEEEE
VESVKAASDIYAPVSGEVIASNADATESPESVNEDAYDAWLFKIKPANADDVNALLSADQ
HHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCCC
YQAKAGA
CCCCCCC
>Mature Secondary Structure 
SNTPVELKYTESHEWVRTEADGTLTIGITDLAQEQLGDIVFLELPDTGRQVKQGEAIAV
CCCCEEEEECCCCCEEEECCCCEEEEEEHHHHHHHHCCEEEEECCCCCCCCCCCCEEEE
VESVKAASDIYAPVSGEVIASNADATESPESVNEDAYDAWLFKIKPANADDVNALLSADQ
HHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCCC
YQAKAGA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852