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The map label for this gene is yfkN [H]

Identifier: 17547970

GI number: 17547970

Start: 3508552

End: 3510270

Strand: Reverse

Name: yfkN [H]

Synonym: RSc3253

Alternate gene names: 17547970

Gene position: 3510270-3508552 (Counterclockwise)

Preceding gene: 17547971

Following gene: 17547969

Centisome position: 94.45

GC content: 71.26

Gene sequence:

>1719_bases
ATGAACCGCCGCGAATTCGTCCAGGCGCTGGCCGTGGCCGCCGCCGCAGGCCTGCACCTGACCGACGCCCGCGCCGCCGA
CACCAGCCTCTACGACCTGCCGCGCTTCGGCAACGTCCACCTGCTGCACTTCACCGACTGCCACGCACAGCTGCTGCCCA
TCGAATACCGCGAGCCCAGCGTCAACCTGGGCGTGGCGCAATGGGCCGGCCAGCCACCGCACCTGGTCGGCCGCGCGCTG
CTCGAGCGCTACGGCATCGCGCCGGGTTCGCGCGCGGCGCACGCCTTCACCGCGCTCGACTTCACCGAGGCGGCGCGCCG
TTTCGGCCGCATGGGCGGCTTCGCGCACCTGGCCACGCTCGTCAAGCGCCTGCGCGCCACGCGCCCGGACGCGCTGCTGC
TCGACGGCGGCGACACCTGGCAGGGCTCGGCCACCTCGCTGTGGACGCGCGGCCAGGACATGATCGACGCCGCGCTCCTG
CTCGGCGTGGACGTCATGACGCCGCACTGGGAGCTGACCTACGGCGCCGAGCGCGTGCGCCACGTGGTCGACCACGACTT
CAGAAACAAGGTCGCCTTCGTCGCCCAGAACATCCAGACCACCGACTTCGGCGACCCGGTGTTCGACCCCTACGTGCTGC
GCGAGCTGAACGGCGTGCCGGTCGCCATCATCGGCCAGGCGTTTCCGTACACGCCCATCGCGCATCCGGCCGATTTCACG
CCGGACTGGACCTTCGGCATCCAGGAAGCGCGGCTGCAGGAGCGGGTGGACGAAGCGCGCGGCAAGGGCGCCCGGGTGGT
CGTGCTGCTGTCGCACAACGGCATGGACGTCGACCTGAAGCTGGCCTCGCGGGTGCGCGGCATCGACGCCATCCTCGGCG
GCCACACACACGATGCGGTGCCGGCACCGGTGCGCGTATCCAACCCCGGCGGCACCACGCTGGTGACCAACGCCGGCTCC
AACGGCAAGTTCGTCGGCGTGCTGGACCTCGACGTGCGCGGCGGCACCGTGCGCGACCTGCGCTACACGCTGCTGCCCGT
CTTCGCCGACCTGCTGCCCCCCGACCCGGCCATGGCCGCGCTGATCGAGCGCGTGCGCGCCCCGTACCGGGACAAGCTCG
GCGAAGTGCTCGCCGTCAACCGCGACCTGCTGTACCGACGCGGCAACTTCAACGGCACCTTCGACCAGCTCATCGTCGAC
GGCCTGATGCAGGCGCAGGGCGCCGAGATCGCCTTTTCGCCCGGCTTCCGCTGGGGCACCACGCTGCTGCCCGGCGAGCC
GCTGACCCTGGAAGCGCTGATGGCCCAGACCGCCATCACCTACCCGGCCACCACGCTCACCGACATGACCGGCGCCACCA
TCAAGACCATCCTGGAAGACGTGGCCGACAACCTGTTCAATCCCGACCCGTACTACCAGCAGGGCGGCGACATGGTGCGT
ACCGGCGGCCTGCGCTACGCCATCGACCCCACCCAGCCCATCGGCCGCCGCATCACCGACCTGCGCCTGGACGACCAGCC
GCTCGATGCTGACCGCCGCTACAAGGTCGCCGGCTGGGCCGCCGTCTCCGCCGAAGCCCGCCGCACCGCCGGCCGCCCCG
TGTGGGACGTGCTGGCCGACTGGCTGCGCGACCAGCACGAAGTGACCGCCCGGCCGCTGAATACACCGCGCATCGTCGGC
GTAGCGGGCAATCCCGGAATCGACACGGAGCGCGGTTGA

Upstream 100 bases:

>100_bases
ACGCCGACGGCCGCGTGGTGCAGAAGGCAACCGGCGAGATGCTCGAAGACGACATCGCCGACCTGGCCCGCTACGCCCAC
GGCACGCAGGCCCCGGCCCG

Downstream 100 bases:

>100_bases
AGGCTGGCTGGGAATTCGGATAGAATCGGCGTCTATTGGGGCGGTAGCTCAGCTGGGAGAGCGTCGCGTTCGCAATGCGA
AGGTCGGGAGTTCGATCCTC

Product: sulfur oxidation signal peptide protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MNRREFVQALAVAAAAGLHLTDARAADTSLYDLPRFGNVHLLHFTDCHAQLLPIEYREPSVNLGVAQWAGQPPHLVGRAL
LERYGIAPGSRAAHAFTALDFTEAARRFGRMGGFAHLATLVKRLRATRPDALLLDGGDTWQGSATSLWTRGQDMIDAALL
LGVDVMTPHWELTYGAERVRHVVDHDFRNKVAFVAQNIQTTDFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFT
PDWTFGIQEARLQERVDEARGKGARVVVLLSHNGMDVDLKLASRVRGIDAILGGHTHDAVPAPVRVSNPGGTTLVTNAGS
NGKFVGVLDLDVRGGTVRDLRYTLLPVFADLLPPDPAMAALIERVRAPYRDKLGEVLAVNRDLLYRRGNFNGTFDQLIVD
GLMQAQGAEIAFSPGFRWGTTLLPGEPLTLEALMAQTAITYPATTLTDMTGATIKTILEDVADNLFNPDPYYQQGGDMVR
TGGLRYAIDPTQPIGRRITDLRLDDQPLDADRRYKVAGWAAVSAEARRTAGRPVWDVLADWLRDQHEVTARPLNTPRIVG
VAGNPGIDTERG

Sequences:

>Translated_572_residues
MNRREFVQALAVAAAAGLHLTDARAADTSLYDLPRFGNVHLLHFTDCHAQLLPIEYREPSVNLGVAQWAGQPPHLVGRAL
LERYGIAPGSRAAHAFTALDFTEAARRFGRMGGFAHLATLVKRLRATRPDALLLDGGDTWQGSATSLWTRGQDMIDAALL
LGVDVMTPHWELTYGAERVRHVVDHDFRNKVAFVAQNIQTTDFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFT
PDWTFGIQEARLQERVDEARGKGARVVVLLSHNGMDVDLKLASRVRGIDAILGGHTHDAVPAPVRVSNPGGTTLVTNAGS
NGKFVGVLDLDVRGGTVRDLRYTLLPVFADLLPPDPAMAALIERVRAPYRDKLGEVLAVNRDLLYRRGNFNGTFDQLIVD
GLMQAQGAEIAFSPGFRWGTTLLPGEPLTLEALMAQTAITYPATTLTDMTGATIKTILEDVADNLFNPDPYYQQGGDMVR
TGGLRYAIDPTQPIGRRITDLRLDDQPLDADRRYKVAGWAAVSAEARRTAGRPVWDVLADWLRDQHEVTARPLNTPRIVG
VAGNPGIDTERG
>Mature_572_residues
MNRREFVQALAVAAAAGLHLTDARAADTSLYDLPRFGNVHLLHFTDCHAQLLPIEYREPSVNLGVAQWAGQPPHLVGRAL
LERYGIAPGSRAAHAFTALDFTEAARRFGRMGGFAHLATLVKRLRATRPDALLLDGGDTWQGSATSLWTRGQDMIDAALL
LGVDVMTPHWELTYGAERVRHVVDHDFRNKVAFVAQNIQTTDFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFT
PDWTFGIQEARLQERVDEARGKGARVVVLLSHNGMDVDLKLASRVRGIDAILGGHTHDAVPAPVRVSNPGGTTLVTNAGS
NGKFVGVLDLDVRGGTVRDLRYTLLPVFADLLPPDPAMAALIERVRAPYRDKLGEVLAVNRDLLYRRGNFNGTFDQLIVD
GLMQAQGAEIAFSPGFRWGTTLLPGEPLTLEALMAQTAITYPATTLTDMTGATIKTILEDVADNLFNPDPYYQQGGDMVR
TGGLRYAIDPTQPIGRRITDLRLDDQPLDADRRYKVAGWAAVSAEARRTAGRPVWDVLADWLRDQHEVTARPLNTPRIVG
VAGNPGIDTERG

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 62494; Mature: 62494

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRREFVQALAVAAAAGLHLTDARAADTSLYDLPRFGNVHLLHFTDCHAQLLPIEYREPS
CCHHHHHHHHHHHHHCCCEEECCCCCCCCHHCCCCCCCEEEEEEECCCCEEEEEEECCCC
VNLGVAQWAGQPPHLVGRALLERYGIAPGSRAAHAFTALDFTEAARRFGRMGGFAHLATL
CCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VKRLRATRPDALLLDGGDTWQGSATSLWTRGQDMIDAALLLGVDVMTPHWELTYGAERVR
HHHHHCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHH
HVVDHDFRNKVAFVAQNIQTTDFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
PDWTFGIQEARLQERVDEARGKGARVVVLLSHNGMDVDLKLASRVRGIDAILGGHTHDAV
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEHHHHHCCHHHHHCCCCCCCC
PAPVRVSNPGGTTLVTNAGSNGKFVGVLDLDVRGGTVRDLRYTLLPVFADLLPPDPAMAA
CCCEEECCCCCCEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHH
LIERVRAPYRDKLGEVLAVNRDLLYRRGNFNGTFDQLIVDGLMQAQGAEIAFSPGFRWGT
HHHHHCCCHHHHHHHHHHCCHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCC
TLLPGEPLTLEALMAQTAITYPATTLTDMTGATIKTILEDVADNLFNPDPYYQQGGDMVR
CCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEE
TGGLRYAIDPTQPIGRRITDLRLDDQPLDADRRYKVAGWAAVSAEARRTAGRPVWDVLAD
ECCEEEEECCCCHHHCCHHCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHH
WLRDQHEVTARPLNTPRIVGVAGNPGIDTERG
HHHCCHHHCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNRREFVQALAVAAAAGLHLTDARAADTSLYDLPRFGNVHLLHFTDCHAQLLPIEYREPS
CCHHHHHHHHHHHHHCCCEEECCCCCCCCHHCCCCCCCEEEEEEECCCCEEEEEEECCCC
VNLGVAQWAGQPPHLVGRALLERYGIAPGSRAAHAFTALDFTEAARRFGRMGGFAHLATL
CCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VKRLRATRPDALLLDGGDTWQGSATSLWTRGQDMIDAALLLGVDVMTPHWELTYGAERVR
HHHHHCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCEEEEHHHHHHH
HVVDHDFRNKVAFVAQNIQTTDFGDPVFDPYVLRELNGVPVAIIGQAFPYTPIAHPADFT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
PDWTFGIQEARLQERVDEARGKGARVVVLLSHNGMDVDLKLASRVRGIDAILGGHTHDAV
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEHHHHHCCHHHHHCCCCCCCC
PAPVRVSNPGGTTLVTNAGSNGKFVGVLDLDVRGGTVRDLRYTLLPVFADLLPPDPAMAA
CCCEEECCCCCCEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHH
LIERVRAPYRDKLGEVLAVNRDLLYRRGNFNGTFDQLIVDGLMQAQGAEIAFSPGFRWGT
HHHHHCCCHHHHHHHHHHCCHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCC
TLLPGEPLTLEALMAQTAITYPATTLTDMTGATIKTILEDVADNLFNPDPYYQQGGDMVR
CCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEE
TGGLRYAIDPTQPIGRRITDLRLDDQPLDADRRYKVAGWAAVSAEARRTAGRPVWDVLAD
ECCEEEEECCCCHHHCCHHCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHH
WLRDQHEVTARPLNTPRIVGVAGNPGIDTERG
HHHCCHHHCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]