BacMap

Definition	Ralstonia solanacearum GMI1000, complete genome.
Accession	NC_003295
Length	3,716,413

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The map label for this gene is yvaG [H]

Identifier: 17547857

GI number: 17547857

Start: 3387988

End: 3388776

Strand: Reverse

Name: yvaG [H]

Synonym: RSc3138

Alternate gene names: 17547857

Gene position: 3388776-3387988 (Counterclockwise)

Preceding gene: 17547859

Following gene: 17547856

Centisome position: 91.18

GC content: 69.46

Gene sequence:

>789_bases
ATGGACCTCCACCTTCAGAACAAGCTCGCTCTCGTGACTGGCTCCACCAAAGGGATCGGCCACGCCATCGCCGTCGCGCT
GGCCGCCGAGGGCGCCCGCGTCATCCTCAACGGCCGCACCGATGCGTCGGTGGCCGAGGCGGTGTCCCGCCTGCTGGCCG
AAGTGCCCGGCGCCCATGCCGAAGCCTTCGCCGGCGACCTGTCCGACCCCGCGCAGGCCCGGGCGCTGGCGGCGCGCTTC
CCGCGGGTGGACATCCTGGTCAACAACCTCGGCATCTTCGACCCCAAGCCGTTCGAGGACATCGACGATGCCGAATGGCT
GCGCTTCTTCAGCGTGAACGTGATGAGCGGCGTGCGCCTGTCGCGTGCCTACCTGCCGGGGATGAAGGCGCAGAACTGGG
GCCGCATCGTCTTCATCAGCAGTGAGAGCGGGGTGCAGATCCCGGCGGAGATGGTCCACTACGGTGTGACCAAGACGGCA
CTGCTCGGCCTGTCGCGCGGGCTGGCCGAACTGTGCGCGGGCACCGCCGTGACGGTCAATGCCGTGCTGCCCGGCCCGAC
GCGCTCCGAAGGGGTCGAGGCCTTCACGCGCAAGCTGTCGGGCGGCCAGGGTTTCGAGGCCTTCGAGAAGGCCTTCTTCG
AGACCGCGCGGCCGACCTCACTGATCAAGCGCTTCGCCACGCCGCAGGAGGTCGCCAGCCTGGTCGCCTATGTGGCCAGC
CCGCTGTCGTCGGCCACCACAGGCGCCGCGCTGCGGGTGGATGGCGGCGTGGTGAAGTCAGCGTTCTGA

Upstream 100 bases:

>100_bases
ACGATTGCGGCAGCCACCGGGCGGGGGCGTTCGTATCATGCTTGAAACGCCGCCGGCGACTGGCGTGTCGCCTCTCCTCC
ATCTTCCTGTTTTTGATGCC

Downstream 100 bases:

>100_bases
GCGCCGCCGTGCCCGCGCGCCGGTCCATGCTCCGCCGCCGCCCGTGTGTGCATCGGACGCGGGTCGGCGCCGCCGGGGCC
GACCGCTCGCCGCATCGCCG

Product: oxidoreductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MDLHLQNKLALVTGSTKGIGHAIAVALAAEGARVILNGRTDASVAEAVSRLLAEVPGAHAEAFAGDLSDPAQARALAARF
PRVDILVNNLGIFDPKPFEDIDDAEWLRFFSVNVMSGVRLSRAYLPGMKAQNWGRIVFISSESGVQIPAEMVHYGVTKTA
LLGLSRGLAELCAGTAVTVNAVLPGPTRSEGVEAFTRKLSGGQGFEAFEKAFFETARPTSLIKRFATPQEVASLVAYVAS
PLSSATTGAALRVDGGVVKSAF

Sequences:

>Translated_262_residues
MDLHLQNKLALVTGSTKGIGHAIAVALAAEGARVILNGRTDASVAEAVSRLLAEVPGAHAEAFAGDLSDPAQARALAARF
PRVDILVNNLGIFDPKPFEDIDDAEWLRFFSVNVMSGVRLSRAYLPGMKAQNWGRIVFISSESGVQIPAEMVHYGVTKTA
LLGLSRGLAELCAGTAVTVNAVLPGPTRSEGVEAFTRKLSGGQGFEAFEKAFFETARPTSLIKRFATPQEVASLVAYVAS
PLSSATTGAALRVDGGVVKSAF
>Mature_262_residues
MDLHLQNKLALVTGSTKGIGHAIAVALAAEGARVILNGRTDASVAEAVSRLLAEVPGAHAEAFAGDLSDPAQARALAARF
PRVDILVNNLGIFDPKPFEDIDDAEWLRFFSVNVMSGVRLSRAYLPGMKAQNWGRIVFISSESGVQIPAEMVHYGVTKTA
LLGLSRGLAELCAGTAVTVNAVLPGPTRSEGVEAFTRKLSGGQGFEAFEKAFFETARPTSLIKRFATPQEVASLVAYVAS
PLSSATTGAALRVDGGVVKSAF

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=258, Percent_Identity=31.7829457364341, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI5031737, Length=256, Percent_Identity=32.8125, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI66933014, Length=258, Percent_Identity=29.4573643410853, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI126723750, Length=260, Percent_Identity=30.7692307692308, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=26.6409266409266, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI126723191, Length=204, Percent_Identity=33.8235294117647, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI33667109, Length=191, Percent_Identity=36.1256544502618, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI7705925, Length=263, Percent_Identity=31.1787072243346, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4504505, Length=191, Percent_Identity=29.8429319371728, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI31542939, Length=210, Percent_Identity=29.5238095238095, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI10190704, Length=256, Percent_Identity=29.296875, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI109715829, Length=246, Percent_Identity=29.6747967479675, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI15277342, Length=276, Percent_Identity=29.3478260869565, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI7706318, Length=199, Percent_Identity=28.1407035175879, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI7705855, Length=183, Percent_Identity=31.6939890710383, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI226371731, Length=182, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI7657478, Length=187, Percent_Identity=32.620320855615, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI224922801, Length=171, Percent_Identity=30.4093567251462, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI223718074, Length=247, Percent_Identity=28.7449392712551, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI20149619, Length=158, Percent_Identity=33.5443037974684, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787335, Length=259, Percent_Identity=35.1351351351351, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=34.4827586206897, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787905, Length=264, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788459, Length=254, Percent_Identity=31.1023622047244, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789378, Length=261, Percent_Identity=31.0344827586207, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790717, Length=258, Percent_Identity=28.2945736434109, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786812, Length=261, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI2367365, Length=251, Percent_Identity=30.6772908366534, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367175, Length=257, Percent_Identity=30.3501945525292, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787526, Length=251, Percent_Identity=28.6852589641434, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082160, Length=270, Percent_Identity=28.5185185185185, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1789208, Length=262, Percent_Identity=26.3358778625954, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787820, Length=239, Percent_Identity=29.2887029288703, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789057, Length=265, Percent_Identity=27.9245283018868, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=263, Percent_Identity=33.4600760456274, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560676, Length=257, Percent_Identity=33.4630350194552, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994604, Length=253, Percent_Identity=32.8063241106719, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=32.421875, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI193204405, Length=267, Percent_Identity=32.9588014981273, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=267, Percent_Identity=31.4606741573034, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17568967, Length=193, Percent_Identity=30.5699481865285, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=31.6793893129771, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17560332, Length=266, Percent_Identity=31.5789473684211, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17562906, Length=263, Percent_Identity=32.6996197718631, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17555706, Length=262, Percent_Identity=31.2977099236641, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=32.319391634981, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25147288, Length=261, Percent_Identity=28.735632183908, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565030, Length=269, Percent_Identity=30.1115241635688, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=267, Percent_Identity=28.4644194756554, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=28.4644194756554, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=26.2357414448669, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=27.4809160305344, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=261, Percent_Identity=28.735632183908, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=171, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508895, Length=189, Percent_Identity=32.8042328042328, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193203115, Length=169, Percent_Identity=31.3609467455621, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=264, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=262, Percent_Identity=27.0992366412214, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=261, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=29.6577946768061, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=192, Percent_Identity=29.1666666666667, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI23397609, Length=256, Percent_Identity=34.765625, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21355319, Length=266, Percent_Identity=30.4511278195489, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=31.640625, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=30.859375, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24644339, Length=258, Percent_Identity=28.2945736434109, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859837, Length=190, Percent_Identity=32.6315789473684, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24643142, Length=253, Percent_Identity=29.2490118577075, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644337, Length=259, Percent_Identity=31.6602316602317, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281364892, Length=176, Percent_Identity=32.9545454545455, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI161076933, Length=182, Percent_Identity=29.6703296703297, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24584748, Length=182, Percent_Identity=29.6703296703297, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21358495, Length=197, Percent_Identity=29.9492385786802, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581726, Length=165, Percent_Identity=29.6969696969697, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 27496; Mature: 27496

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLHLQNKLALVTGSTKGIGHAIAVALAAEGARVILNGRTDASVAEAVSRLLAEVPGAHA
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHH
EAFAGDLSDPAQARALAARFPRVDILVNNLGIFDPKPFEDIDDAEWLRFFSVNVMSGVRL
HHHHCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHH
SRAYLPGMKAQNWGRIVFISSESGVQIPAEMVHYGVTKTALLGLSRGLAELCAGTAVTVN
HHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEE
AVLPGPTRSEGVEAFTRKLSGGQGFEAFEKAFFETARPTSLIKRFATPQEVASLVAYVAS
EECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHH
PLSSATTGAALRVDGGVVKSAF
HHCCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MDLHLQNKLALVTGSTKGIGHAIAVALAAEGARVILNGRTDASVAEAVSRLLAEVPGAHA
CCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHH
EAFAGDLSDPAQARALAARFPRVDILVNNLGIFDPKPFEDIDDAEWLRFFSVNVMSGVRL
HHHHCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHH
SRAYLPGMKAQNWGRIVFISSESGVQIPAEMVHYGVTKTALLGLSRGLAELCAGTAVTVN
HHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEE
AVLPGPTRSEGVEAFTRKLSGGQGFEAFEKAFFETARPTSLIKRFATPQEVASLVAYVAS
EECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHH
PLSSATTGAALRVDGGVVKSAF
HHCCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]