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The map label for this gene is ldhA [H]

Identifier: 17547850

GI number: 17547850

Start: 3377711

End: 3378739

Strand: Direct

Name: ldhA [H]

Synonym: RSc3131

Alternate gene names: 17547850

Gene position: 3377711-3378739 (Clockwise)

Preceding gene: 17547849

Following gene: 17547851

Centisome position: 90.89

GC content: 70.85

Gene sequence:

>1029_bases
GTGCCGCATCACCGGCTTCCCTCACCCACCGTCATGCGCATCCTCTTCTTCAGCAGCCACCGTTACGACGAAGACGCCTT
CCGCGCCGCGCAGGCGCGCGGCGGCCACGGCTTCGAGCTGGTCTTCCAGCGCGCCCACCTGGACGTGCACACCGCACCGC
TGGCCGCCGGCTTCGAGGTGGTCTGCCCGTTCGTCAACGACTGCCTGGATGCCGGCGTGCTCGCCGTGCTGGCCGCGGGC
GGCGCCCGGCTGATCGCGCTGCGCTCGGCCGGCTTCAACCATGTCGACCTGGCCGCGGCGCAGCGGCTGGGGCTGACGGT
GGTGCGGGTGCCGGCGTATTCGCCGCACGCGGTGGCCGAGCACGCGGTCGGCATGATCCTCACGCTCAACCGGCGCCTGC
ACCGCGCCTGCAACCGCACCCGCGAGGGCGACTTCTCGCTCGACGGGCTGCTCGGCTTCGACCTGGCCGGCAAGACCGTC
GGCGTGGTCGGCACCGGGCAGATCGGGCAGGTGTTCGCGCGCATCATGGCCGGCTTCGGCTGCCGGCTGCTGGCGTTCGA
TCCGTTCCCGCAGCCGGCGCTGGCGGCGCTCGGCGTGACCTACGTGCCGCTGCCGGCTCTGCTGGCGCAGTCCGACATCG
TCAGCCTGCACTGCCCGCTCAACGCCGACACGCACCACCTGATCGACGCCGGCGCACTCGCCAGCATGAAGACGGGCGCC
ATGCTGATCAACACCAGCCGCGGCGGGCTGGTCGACAGCCCCGCACTGATCGACGCCCTCAAATCCGGCCAGCTCGGCCA
CCTCGGACTGGATGTTTATGAGGAAGAGGCCGACCTGTTCTTCGAGGACCGCTCCGCCGATGTGCTGCAGGACGACGTGC
TGGCGCGCCTGCTGACCTTCCCCAATGTCATCGTGACCGCGCACCAGGCCTTCTTCACGCGCGAGGCGCTGGCGGGCATT
GCCGACACCACGCTCGCCAACGTGGCGGCATGGGCAGCCGGCGCGCCCGCGAATGTGGTGAAGGCTTAG

Upstream 100 bases:

>100_bases
ATCAACCAGGTCCTCGCCGACCTGAAAGCCGGCAAGGTGGACGGCCGCATCGTCCTCTCGCTGGACTGAGCAGCACGCCG
TCACGGGGTGCGGGTAAGCT

Downstream 100 bases:

>100_bases
CCCGCACACGCACACTGAGACGTTCCGATCGCACGCACAGGCCACAAGCTGGCCGAGACGATCGTTGACGATCAAATCCA
GTTTACTGGATAATGAATCT

Product: D-lactate dehydrogenase

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MPHHRLPSPTVMRILFFSSHRYDEDAFRAAQARGGHGFELVFQRAHLDVHTAPLAAGFEVVCPFVNDCLDAGVLAVLAAG
GARLIALRSAGFNHVDLAAAQRLGLTVVRVPAYSPHAVAEHAVGMILTLNRRLHRACNRTREGDFSLDGLLGFDLAGKTV
GVVGTGQIGQVFARIMAGFGCRLLAFDPFPQPALAALGVTYVPLPALLAQSDIVSLHCPLNADTHHLIDAGALASMKTGA
MLINTSRGGLVDSPALIDALKSGQLGHLGLDVYEEEADLFFEDRSADVLQDDVLARLLTFPNVIVTAHQAFFTREALAGI
ADTTLANVAAWAAGAPANVVKA

Sequences:

>Translated_342_residues
MPHHRLPSPTVMRILFFSSHRYDEDAFRAAQARGGHGFELVFQRAHLDVHTAPLAAGFEVVCPFVNDCLDAGVLAVLAAG
GARLIALRSAGFNHVDLAAAQRLGLTVVRVPAYSPHAVAEHAVGMILTLNRRLHRACNRTREGDFSLDGLLGFDLAGKTV
GVVGTGQIGQVFARIMAGFGCRLLAFDPFPQPALAALGVTYVPLPALLAQSDIVSLHCPLNADTHHLIDAGALASMKTGA
MLINTSRGGLVDSPALIDALKSGQLGHLGLDVYEEEADLFFEDRSADVLQDDVLARLLTFPNVIVTAHQAFFTREALAGI
ADTTLANVAAWAAGAPANVVKA
>Mature_341_residues
PHHRLPSPTVMRILFFSSHRYDEDAFRAAQARGGHGFELVFQRAHLDVHTAPLAAGFEVVCPFVNDCLDAGVLAVLAAGG
ARLIALRSAGFNHVDLAAAQRLGLTVVRVPAYSPHAVAEHAVGMILTLNRRLHRACNRTREGDFSLDGLLGFDLAGKTVG
VVGTGQIGQVFARIMAGFGCRLLAFDPFPQPALAALGVTYVPLPALLAQSDIVSLHCPLNADTHHLIDAGALASMKTGAM
LINTSRGGLVDSPALIDALKSGQLGHLGLDVYEEEADLFFEDRSADVLQDDVLARLLTFPNVIVTAHQAFFTREALAGIA
DTTLANVAAWAAGAPANVVKA

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=240, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI61743967, Length=246, Percent_Identity=36.5853658536585, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI145580578, Length=243, Percent_Identity=35.3909465020576, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI4557499, Length=243, Percent_Identity=35.3909465020576, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI4557497, Length=246, Percent_Identity=36.5853658536585, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI145580575, Length=243, Percent_Identity=35.3909465020576, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI6912396, Length=208, Percent_Identity=36.0576923076923, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1787645, Length=323, Percent_Identity=54.4891640866873, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI87082289, Length=268, Percent_Identity=30.2238805970149, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789279, Length=231, Percent_Identity=33.7662337662338, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788660, Length=212, Percent_Identity=31.6037735849057, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=266, Percent_Identity=27.8195488721804, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25147481, Length=233, Percent_Identity=29.6137339055794, Blast_Score=95, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324055, Length=199, Percent_Identity=34.6733668341709, Blast_Score=119, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=232, Percent_Identity=33.6206896551724, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=232, Percent_Identity=33.1896551724138, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=32.0754716981132, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=33.5714285714286, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24646446, Length=241, Percent_Identity=38.1742738589212, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24646448, Length=241, Percent_Identity=38.1742738589212, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24646452, Length=241, Percent_Identity=38.1742738589212, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24646450, Length=241, Percent_Identity=38.1742738589212, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI62472511, Length=241, Percent_Identity=38.1742738589212, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI19921140, Length=249, Percent_Identity=30.9236947791165, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45552429, Length=268, Percent_Identity=30.9701492537313, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585514, Length=268, Percent_Identity=30.9701492537313, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574282, Length=268, Percent_Identity=30.9701492537313, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574286, Length=278, Percent_Identity=30.9352517985612, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45551003, Length=268, Percent_Identity=30.9701492537313, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574284, Length=268, Percent_Identity=30.9701492537313, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28571528, Length=274, Percent_Identity=30.6569343065693, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585516, Length=213, Percent_Identity=26.7605633802817, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36173; Mature: 36042

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHHRLPSPTVMRILFFSSHRYDEDAFRAAQARGGHGFELVFQRAHLDVHTAPLAAGFEV
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHEEEHHCCCEECCCHHCCHHH
VCPFVNDCLDAGVLAVLAAGGARLIALRSAGFNHVDLAAAQRLGLTVVRVPAYSPHAVAE
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCHHHHH
HAVGMILTLNRRLHRACNRTREGDFSLDGLLGFDLAGKTVGVVGTGQIGQVFARIMAGFG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHCCC
CRLLAFDPFPQPALAALGVTYVPLPALLAQSDIVSLHCPLNADTHHLIDAGALASMKTGA
CEEEEECCCCCHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCE
MLINTSRGGLVDSPALIDALKSGQLGHLGLDVYEEEADLFFEDRSADVLQDDVLARLLTF
EEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHC
PNVIVTAHQAFFTREALAGIADTTLANVAAWAAGAPANVVKA
CHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure 
PHHRLPSPTVMRILFFSSHRYDEDAFRAAQARGGHGFELVFQRAHLDVHTAPLAAGFEV
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHEEEHHCCCEECCCHHCCHHH
VCPFVNDCLDAGVLAVLAAGGARLIALRSAGFNHVDLAAAQRLGLTVVRVPAYSPHAVAE
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCHHHHH
HAVGMILTLNRRLHRACNRTREGDFSLDGLLGFDLAGKTVGVVGTGQIGQVFARIMAGFG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEEECCHHHHHHHHHHHCCC
CRLLAFDPFPQPALAALGVTYVPLPALLAQSDIVSLHCPLNADTHHLIDAGALASMKTGA
CEEEEECCCCCHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCE
MLINTSRGGLVDSPALIDALKSGQLGHLGLDVYEEEADLFFEDRSADVLQDDVLARLLTF
EEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHC
PNVIVTAHQAFFTREALAGIADTTLANVAAWAAGAPANVVKA
CHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]