Click here to switch to the map view.

The map label for this gene is aroK

Identifier: 17547689

GI number: 17547689

Start: 3202401

End: 3202973

Strand: Reverse

Name: aroK

Synonym: RSc2970

Alternate gene names: 17547689

Gene position: 3202973-3202401 (Counterclockwise)

Preceding gene: 17547690

Following gene: 17547688

Centisome position: 86.18

GC content: 65.79

Gene sequence:

>573_bases
TTGTCTGTTTCCAACGTTTTTTTCGTCGGCCTGATGGGGGCCGGCAAGACCACTGTCGGGCGTGCCGTCGCCCGGCGGCT
TGACTTGCCGTTCTTCGACTCCGACCACGAGATCGAAGCGCGTTGCGGCGTGCGCGTGCCGGTCATCTTCGAGCATGAAG
GCGAAATGGGCTTTCGCGACCGCGAAACCCAGATGATCGATGAGCTGACCGCGCGCCACGGCGTGGTCGTGGCCACCGGC
GGCGGCGCCGTGCTGCGCCCGGAAAACCGCGCTTTCCTGCGCGAGCGCGGCACGGTGATCTACCTGCGCGCCAATCCCCA
CGACCTGTACCTGCGCACGCGCCACGACAAGAACCGTCCGCTGCTGCAGACCGAGAACCCGCGCGCCAGGCTCGAGGAAC
TGCACGCGATTCGCGACCCGCTGTACCGCGAGGTGGCGCACTTTGTCATCGAAACCGGCAAGCCCACCGTGGCCCAGCTT
GTTAATATGGTGCTGATGCAACTCGAAGTGGCCGGCATCGTGGTGCCGCCCGCCGCCTCTTCCCCGACCTCGCAAGTCTC
CCGCCAGTCATGA

Upstream 100 bases:

>100_bases
GATGGAGTGCCGGGCTTTTTTTCGTTCGGCGCATCGATCGGCGCGGGATGCCGGGCAAAAGCCACTACAATCGGAATCAA
ATTTCAGATTTCGCGCGATT

Downstream 100 bases:

>100_bases
TTACCGTTGATGTCGACCTGGGCGAGCGCGCCTATCCGATCCATATCGGAACCGGGCTCCTGTCCCAAGCCGAACTGTTT
GCCCCCCATATCCGCGGCAC

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSVSNVFFVGLMGAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPVIFEHEGEMGFRDRETQMIDELTARHGVVVATG
GGAVLRPENRAFLRERGTVIYLRANPHDLYLRTRHDKNRPLLQTENPRARLEELHAIRDPLYREVAHFVIETGKPTVAQL
VNMVLMQLEVAGIVVPPAASSPTSQVSRQS

Sequences:

>Translated_190_residues
MSVSNVFFVGLMGAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPVIFEHEGEMGFRDRETQMIDELTARHGVVVATG
GGAVLRPENRAFLRERGTVIYLRANPHDLYLRTRHDKNRPLLQTENPRARLEELHAIRDPLYREVAHFVIETGKPTVAQL
VNMVLMQLEVAGIVVPPAASSPTSQVSRQS
>Mature_189_residues
SVSNVFFVGLMGAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPVIFEHEGEMGFRDRETQMIDELTARHGVVVATGG
GAVLRPENRAFLRERGTVIYLRANPHDLYLRTRHDKNRPLLQTENPRARLEELHAIRDPLYREVAHFVIETGKPTVAQLV
NMVLMQLEVAGIVVPPAASSPTSQVSRQS

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=166, Percent_Identity=47.5903614457831, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1786587, Length=155, Percent_Identity=38.7096774193548, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_RALSO (Q8XV61)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_521091.1
- ProteinModelPortal:   Q8XV61
- SMR:   Q8XV61
- GeneID:   1221824
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2970
- NMPDR:   fig|267608.1.peg.2970
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- ProtClustDB:   PRK00131
- BioCyc:   RSOL267608:RSC2970-MONOMER
- BRENDA:   2.7.1.71
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 21221; Mature: 21089

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS01128 SHIKIMATE_KINASE; PS00267 TACHYKININ

Important sites: BINDING 35-35 BINDING 59-59 BINDING 81-81 BINDING 119-119 BINDING 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSNVFFVGLMGAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPVIFEHEGEMGFRD
CCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEECCCCCCCCC
RETQMIDELTARHGVVVATGGGAVLRPENRAFLRERGTVIYLRANPHDLYLRTRHDKNRP
HHHHHHHHHHHHCCEEEEECCCEEECCCCHHHHHHCCCEEEEEECCCEEEEEECCCCCCC
LLQTENPRARLEELHAIRDPLYREVAHFVIETGKPTVAQLVNMVLMQLEVAGIVVPPAAS
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCCC
SPTSQVSRQS
CCHHHHCCCC
>Mature Secondary Structure 
SVSNVFFVGLMGAGKTTVGRAVARRLDLPFFDSDHEIEARCGVRVPVIFEHEGEMGFRD
CCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEECCCCCCCCC
RETQMIDELTARHGVVVATGGGAVLRPENRAFLRERGTVIYLRANPHDLYLRTRHDKNRP
HHHHHHHHHHHHCCEEEEECCCEEECCCCHHHHHHCCCEEEEEECCCEEEEEECCCCCCC
LLQTENPRARLEELHAIRDPLYREVAHFVIETGKPTVAQLVNMVLMQLEVAGIVVPPAAS
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCCC
SPTSQVSRQS
CCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852