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The map label for this gene is mlaE [H]

Identifier: 17547680

GI number: 17547680

Start: 3188679

End: 3189446

Strand: Reverse

Name: mlaE [H]

Synonym: RSc2961

Alternate gene names: 17547680

Gene position: 3189446-3188679 (Counterclockwise)

Preceding gene: 17547681

Following gene: 17547679

Centisome position: 85.82

GC content: 66.67

Gene sequence:

>768_bases
ATGATTTCGTCGATCGGCTTCCTGGTTCGCATCCTGATCAGCCGCCTCGGCTACGCGACACGCATGCTGTTGGCGCTGAT
CGGCGCCGGCCGCGACGTGCTGCGCCGCCCGCGCCTGCTGGTCGAGCAGTTGCGTTTCATCGGCAACGACTCGTTCATCA
TCATCGCGGTATCGGGCCTGTTCGTCGGCTTCGTGCTGGGCCTGCAGGGCTACTACACGCTCAACCGCTACGGCTCGGAG
CAGGCGCTGGGCCTGCTGGTGGCGCTGTCGCTGGTGCGCGAGCTGGGGCCGGTGGTGACGGCGCTGCTGTTCGCCGGCCG
CGCCGGCACTTCGCTGACCGCCGAGATCGGCCTGATGAAGGCCAACGAAGAGCTGATCGCCATGGAAATGATGGCGGTCG
ATCCTCTTGCCCGCGTGCTGGCGCCGCGCTTCTGGGGCGGCTTCATCGCCATGCCGTTGCTGGCCGCCATCTTCAGCGCG
GTGGGCATTCTGGGCGGATACTTCGTCGGCGTGGGCCTGATCGGCGTGGATGCCGGCGCATTCTGGTCGCAGATGCAGGC
CGGCGTGGACGTGGTGAAAGACGTGCTCAACGGCGTCATCAAGAGCGTGGTGTTCGGCGTGGCGGTCACCTTCGTCGCGC
TGTACCAGGGCTATGAGGCCAAGGCCACGCCCGAGGGCGTGTCGCGTGCGACCACCCGCACCGTGGTGATTGCGTCGCTG
GCCGTGCTGGCGTTGGACTTCGTGCTGACCGCGCTGATGTTCAGTTGA

Upstream 100 bases:

>100_bases
TCGTGCACGGCGAGCCCGACGGCCCCGTGCCGTTCCATTACCCGGGCGTGCCCCTGGCCGAAGACTTCGGCGTAGGTGGC
CGGCGTGACGGGGAGGCGCA

Downstream 100 bases:

>100_bases
AGCGGTCGGCATTCAGAGAGAAAAGATCATGCGTAAGAGCCTCCTCGATTTCTGGGTCGGACTGTTCGTGCTGGTGGGCC
TCATCGCGCTGCTGTTCCTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MISSIGFLVRILISRLGYATRMLLALIGAGRDVLRRPRLLVEQLRFIGNDSFIIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKANEELIAMEMMAVDPLARVLAPRFWGGFIAMPLLAAIFSA
VGILGGYFVGVGLIGVDAGAFWSQMQAGVDVVKDVLNGVIKSVVFGVAVTFVALYQGYEAKATPEGVSRATTRTVVIASL
AVLALDFVLTALMFS

Sequences:

>Translated_255_residues
MISSIGFLVRILISRLGYATRMLLALIGAGRDVLRRPRLLVEQLRFIGNDSFIIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKANEELIAMEMMAVDPLARVLAPRFWGGFIAMPLLAAIFSA
VGILGGYFVGVGLIGVDAGAFWSQMQAGVDVVKDVLNGVIKSVVFGVAVTFVALYQGYEAKATPEGVSRATTRTVVIASL
AVLALDFVLTALMFS
>Mature_255_residues
MISSIGFLVRILISRLGYATRMLLALIGAGRDVLRRPRLLVEQLRFIGNDSFIIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKANEELIAMEMMAVDPLARVLAPRFWGGFIAMPLLAAIFSA
VGILGGYFVGVGLIGVDAGAFWSQMQAGVDVVKDVLNGVIKSVVFGVAVTFVALYQGYEAKATPEGVSRATTRTVVIASL
AVLALDFVLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=220, Percent_Identity=63.6363636363636, Blast_Score=272, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27039; Mature: 27039

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSIGFLVRILISRLGYATRMLLALIGAGRDVLRRPRLLVEQLRFIGNDSFIIIAVSGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHH
FVGFVLGLQGYYTLNRYGSEQALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
ANEELIAMEMMAVDPLARVLAPRFWGGFIAMPLLAAIFSAVGILGGYFVGVGLIGVDAGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FWSQMQAGVDVVKDVLNGVIKSVVFGVAVTFVALYQGYEAKATPEGVSRATTRTVVIASL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AVLALDFVLTALMFS
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MISSIGFLVRILISRLGYATRMLLALIGAGRDVLRRPRLLVEQLRFIGNDSFIIIAVSGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHH
FVGFVLGLQGYYTLNRYGSEQALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
ANEELIAMEMMAVDPLARVLAPRFWGGFIAMPLLAAIFSAVGILGGYFVGVGLIGVDAGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FWSQMQAGVDVVKDVLNGVIKSVVFGVAVTFVALYQGYEAKATPEGVSRATTRTVVIASL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
AVLALDFVLTALMFS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]