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The map label for this gene is prfA [H]

Identifier: 17547620

GI number: 17547620

Start: 3128454

End: 3129536

Strand: Direct

Name: prfA [H]

Synonym: RSc2901

Alternate gene names: 17547620

Gene position: 3128454-3129536 (Clockwise)

Preceding gene: 17547619

Following gene: 17547621

Centisome position: 84.18

GC content: 68.79

Gene sequence:

>1083_bases
ATGAAGCCCAGCATGCTGTCCAAGCTCGACCAACTGGCCGAGCGCACCGTCGAAATCAACGCGCTGCTCGCGCGCGAAGA
CGCCACCGCCAACCTCGACCAGTACCGCAAGCTCACGCGCGAACACGCCGAGCTCGAACCCGTGGTGGCGCAGTACGCCG
CATGGAAACAGGCCGAGGACGACGTCACCACCGCGCAGGAACTGCTGGCCGACCCCGACATGAAGGCGTTCGCCGAAGAC
GAGATCCGCGCGGCGCGCGAACGCATGACCACGCTCGAGGCCGAGCTGCAGCGCCTGCTGCTGCCCAAGGACCCGAACGA
CCATCGCAACATCTTCCTGGAAATCCGCGCCGGCACGGGCGGCGACGAGTCCGCGCTGTTCGCTGGCGACCTGCTGCGCA
TGTACACGCGCTACGCCGAACGCCAGCGCTGGCAGGTGGAGATCGTCAGCGAATCGGCGTCGGACCTCGGCGGCTACAAG
GAAGTGATTGTCCGGCTGGTGGGCGAAGGTGCCTACTCGCGGCTGAAATTCGAGTCGGGCGGCCACCGCGTGCAGCGCGT
GCCGGCCACCGAAGCGCAGGGCCGCATCCACACCTCCGCCTGCACCGTGGCCGTGATGCCCGAGGCCGACCCGCTCGCCG
ACATCCAGATCAACCCGGCCGACCTGCGCATCGACACCTTCCGCGCGTCGGGCGCGGGCGGCCAGCACATCAACAAGACC
GACTCGGCGGTGCGCCTCACCCACCTGCCCACCGGCCTGGTGGTGGAATGCCAGGACGACCGCAGCCAGCACCGCAACAA
GGACCGCGCCATGCAAGTGCTGGCCGCGCGCCTGAAGGACCGCCAGGAGCGCGAGGCGCAGGCCAAGGAGGCCAGCGCGC
GCAAGAGCCTGATCGGCTCGGGCGACCGCAGCGACCGCATCCGCACGTACAACTTCCCGCAGGGCCGCATCACCGACCAC
CGCATCAACCTGACGCTGTACAAGATCGATGCGCTCATGGACGGCGACCTGGGCGAACTGCTGGGTGCGCTGGCCGCCGA
GCATCAGGCCGAACAGCTGGCCGCCCTCGGTGAAGAGGCCTGA

Upstream 100 bases:

>100_bases
CTAGCGGCGCTTGCCATCGCCGTGCGCGCCGCCCGGGCGCGTGCCTTCGGCGCGGTATCATCGCCGCCCGCGTCCTGTGT
TCCCTTCCGCGACTGCCCGA

Downstream 100 bases:

>100_bases
TGGTCAGATTGTTAAAAAGCGCTGGCCCCGTAACATCTGCTTGCGTTTGAAACAAGGTTGTGCGGTGGCACACCGGCGCC
CCCTTCCTATAATCATCGTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKPSMLSKLDQLAERTVEINALLAREDATANLDQYRKLTREHAELEPVVAQYAAWKQAEDDVTTAQELLADPDMKAFAED
EIRAARERMTTLEAELQRLLLPKDPNDHRNIFLEIRAGTGGDESALFAGDLLRMYTRYAERQRWQVEIVSESASDLGGYK
EVIVRLVGEGAYSRLKFESGGHRVQRVPATEAQGRIHTSACTVAVMPEADPLADIQINPADLRIDTFRASGAGGQHINKT
DSAVRLTHLPTGLVVECQDDRSQHRNKDRAMQVLAARLKDRQEREAQAKEASARKSLIGSGDRSDRIRTYNFPQGRITDH
RINLTLYKIDALMDGDLGELLGALAAEHQAEQLAALGEEA

Sequences:

>Translated_360_residues
MKPSMLSKLDQLAERTVEINALLAREDATANLDQYRKLTREHAELEPVVAQYAAWKQAEDDVTTAQELLADPDMKAFAED
EIRAARERMTTLEAELQRLLLPKDPNDHRNIFLEIRAGTGGDESALFAGDLLRMYTRYAERQRWQVEIVSESASDLGGYK
EVIVRLVGEGAYSRLKFESGGHRVQRVPATEAQGRIHTSACTVAVMPEADPLADIQINPADLRIDTFRASGAGGQHINKT
DSAVRLTHLPTGLVVECQDDRSQHRNKDRAMQVLAARLKDRQEREAQAKEASARKSLIGSGDRSDRIRTYNFPQGRITDH
RINLTLYKIDALMDGDLGELLGALAAEHQAEQLAALGEEA
>Mature_360_residues
MKPSMLSKLDQLAERTVEINALLAREDATANLDQYRKLTREHAELEPVVAQYAAWKQAEDDVTTAQELLADPDMKAFAED
EIRAARERMTTLEAELQRLLLPKDPNDHRNIFLEIRAGTGGDESALFAGDLLRMYTRYAERQRWQVEIVSESASDLGGYK
EVIVRLVGEGAYSRLKFESGGHRVQRVPATEAQGRIHTSACTVAVMPEADPLADIQINPADLRIDTFRASGAGGQHINKT
DSAVRLTHLPTGLVVECQDDRSQHRNKDRAMQVLAARLKDRQEREAQAKEASARKSLIGSGDRSDRIRTYNFPQGRITDH
RINLTLYKIDALMDGDLGELLGALAAEHQAEQLAALGEEA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=47.6027397260274, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI34577120, Length=343, Percent_Identity=39.6501457725948, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI166795305, Length=197, Percent_Identity=45.6852791878173, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=62.2222222222222, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=39.5280235988201, Blast_Score=224, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=316, Percent_Identity=33.8607594936709, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=40.4255319148936, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI19921226, Length=341, Percent_Identity=41.0557184750733, Blast_Score=233, Evalue=1e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40077; Mature: 40077

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSMLSKLDQLAERTVEINALLAREDATANLDQYRKLTREHAELEPVVAQYAAWKQAED
CCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVTTAQELLADPDMKAFAEDEIRAARERMTTLEAELQRLLLPKDPNDHRNIFLEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYTRYAERQRWQVEIVSESASDLGGYKEVIVRLVGEGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHCCHHHHHHHHHHCCCHHHEEEECCC
GHRVQRVPATEAQGRIHTSACTVAVMPEADPLADIQINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRLTHLPTGLVVECQDDRSQHRNKDRAMQVLAARLKDRQEREAQAKEASARKSLIGS
CCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSDRIRTYNFPQGRITDHRINLTLYKIDALMDGDLGELLGALAAEHQAEQLAALGEEA
CCCCCCEEEECCCCCCCCCEEEEEEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPSMLSKLDQLAERTVEINALLAREDATANLDQYRKLTREHAELEPVVAQYAAWKQAED
CCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVTTAQELLADPDMKAFAEDEIRAARERMTTLEAELQRLLLPKDPNDHRNIFLEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
GDESALFAGDLLRMYTRYAERQRWQVEIVSESASDLGGYKEVIVRLVGEGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHCCHHHHHHHHHHCCCHHHEEEECCC
GHRVQRVPATEAQGRIHTSACTVAVMPEADPLADIQINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRLTHLPTGLVVECQDDRSQHRNKDRAMQVLAARLKDRQEREAQAKEASARKSLIGS
CCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSDRIRTYNFPQGRITDHRINLTLYKIDALMDGDLGELLGALAAEHQAEQLAALGEEA
CCCCCCEEEECCCCCCCCCEEEEEEEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA