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The map label for this gene is fruB [H]

Identifier: 17547580

GI number: 17547580

Start: 3081592

End: 3084126

Strand: Direct

Name: fruB [H]

Synonym: RSc2861

Alternate gene names: 17547580

Gene position: 3081592-3084126 (Clockwise)

Preceding gene: 17547575

Following gene: 17547581

Centisome position: 82.92

GC content: 74.0

Gene sequence:

>2535_bases
ATGCCGTCCCCCCTCATCTGCGCCGAGCGCATCCGCCTGCAGCAGCGCGCCACCGACAAGACCAGCGCCATCCGGGCCGC
CGGCCGGCTGCTGGCCGACACCGGGTGCATCGACCCGGCGTACATCGACAGCCTGCTGCGCCGCGAGACGGTGGCCAACA
CCTTCCTCGGCCACGGCGTCGCCATTCCGCACGGCATGGGCGAGGACCGCCACCTGATCCGCCAGACCGGCATCGCGGTG
CTGCAGTTTCCCGACGGGCTGGAATGGCACCCGGGCCAGACCACGCACCTAGTGTTTGCGATTGCGGCGCAGTCGGACGA
GCACATCACGCTGCTGCGCCGGCTGACCCGCCTGCTGAACGACGACGCGCGTCTGCGCCAGCTGTTTTCCACGCAGCGGG
CGGAGGACATCGTCGCCGCGCTGTCGCAGGATGCGCCGGCCCCGGCGGCAAGCGCTCCGGGCGGCGATCTGGCCGAACGG
CTGGCGCTGACACTGAACTACCCCAGCGGCCTGCACGCCCGCCCCGCTGCTCAGTGGGTCGAGACCGCGCGCCGGTTCGC
CGCGCGCGTGCAGGTCCGCCATGGCGCGGAGACAGCCGATGCCAAGAACCTGGTGGCGCTGCTGCAGCTGGGGCTGGCCG
CCGGCGCCGCGCTCACGCTGTCGGCCGAGGGGCCGGATGCCCGCGCCGCGCTGACGGCGCTGCAGCACACCATCCGCAGC
CTGACGGCCCAGGAGCGCGCCCAGGCCGCACGCGACACGCAAGCCGCCCGTACGCGCCAGATCGCACGCGGCTGGCAGCC
CGCGGCGGACCTGCCCGCCATCGGCGGCATCGGCGCGAGCCCCGGGCTGGCCATCGGCACCGTCCACGTCATGCAGCCGG
GGGTCTCGGCGATACCCGACCATCCGGTTCCGCTGGCCACGGGCGGCGATCTGCTGGACCGCGCCCTGGCCGACACGCGC
GCCGAACTGGCTGCGCTCGCCCGCGACACCGCCGCGCGGCTGGGCGAAGCCGAGGCCGGCATCTTCAAGGCCCAGGCCGA
ACTGCTGGGCGACACCGACCTGATGACGCTGACCTGCCAGGCGATGGTCGAGGGCCACGGCGTGGCATGGTCGTGGCATC
ACGCCGTGGAGCGGCTGGCCGAGCGGCTGGCGGCGCTGGGCAATCCGCTGCTGGCCGCGCGCGCCGCCGACCTGCGCGAT
GTGGGCCGGCGCGTGCTCGGCCATCTCGATCCCGCCCTGCGCGGCACGGCGCAGGCGCTGCCGGACGGGCCGTGCATCTT
GGTGGCGCAAGACCTGTCGCCGTCGGACACCGCCGCCCTGGATACCCGCCGCATCGCCGGCATCGTCACCGCGCAGGGCG
GCCCCACCTCGCATACCGCCATCCTGACGCGCACGCTGGGCATTCCCGCAGTGGTCGCGGCCGGGCCGGCCGTGCTGGAC
GTGGCCTCCGGCACACAGGCCATCGTCGACGGCTCCGGCGGCCAGCTGTATCTCGACCCGGATGCCGCCGCCGTCGCCGG
CGCCGAGGCCTGGCTGCGGGAAGACGCCGCGCGGGCGCAGCGCGAGCAGGCCGAACGAGGCCTGCCGGCCCGCACGCGCG
ACGGCCACGCGGTGGAGATCGCCGCCAACGTCAACCTGCCCGCGCAAGCCATCGAGGCCGTCACCCTGGGCGCCGAAGGC
GTGGGCCTGATGCGCACCGAGTTCCTCTTCCTCGAGCGCGACCATGCCCCGGACGAAGACGCGCAGCACGAGGTCTATGC
CGCCATGCTCGGCGCCCTGGGCGGCCGTCCGCTGATCGTGCGCACGCTCGACATCGGCGGCGACAAGCAGGTCCCGCACC
TGAACCTGCCCAAGGAGGAGAACCCCTTCCTGGGCGTGCGCGGCGCGCGGCTGCTGCTGCGCCGGCCCGACCTGATGGAG
CCGCAGCTGCGCGCGCTCTACCGCGCGGCCAGCGGCGGCGGCCCGCTGTCCATCATGTTCCCGATGATCACGTCGCTCGG
CGAGGTGATCGCCCTGCGCGCCGCCTGCGAGCGCATCCGCGCCGAACTCGATGCGCCGGCGGTGCCGCTGGGCATCATGG
TGGAAGTGCCGGCCGCCGCGCTGCTGGCCGACCAGTTGGCCGAGCACGTCGATTTCTTCTCGATCGGCACCAACGACCTC
ACGCAGTACACGCTGGCGATCGACCGCCAGCACCCCGACCTTGCCGCCGAGGCCGACAGCCTGCACCCCGCCGTGCTGCG
GCTGATCCAGTTGACCGTGCAAGGCGCGGCGCGGCACGGCCGCTGGGTGGGCGTGTGCGGCGGACTGGCCGGCGACCCGT
TCGGCGCCCTGCTGCTGACGGGCCTGGGCGTGCACGAACTGTCGATGAGCCCGCGGGACATCCCCGCCGTGAAAGCGCGC
CTGCGCGATGCCCATGTCCGGCAACTGACCGAGCTGGCCGGGCAGGCGCTCGCCTGCGCATCGGCCGAAGACGTGCGGGC
GCTCGAAGCCACCCTCCCCGCTCATCGCGCCGGCACGGAGATGGCCGCAGCATGA

Upstream 100 bases:

>100_bases
CCGCACATTGACACGGACAATGTAATCGATTTCAATCCGACCACATCAACGACACGCCCGCCGCGCCCGCGAGCCCGGCG
ATCGCAGGAGACACCGTCGC

Downstream 100 bases:

>100_bases
CCCAGCGCATCGTCACCGTCACCCTGAATCCGGCCATCGACATGACGGTCGGCCTGGATCGGCTCGAACGCGGCCATGTC
AACCTCGGCCGCAGCGTCAC

Product: multiphosphoryl transfer protein

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 844; Mature: 843

Protein sequence:

>844_residues
MPSPLICAERIRLQQRATDKTSAIRAAGRLLADTGCIDPAYIDSLLRRETVANTFLGHGVAIPHGMGEDRHLIRQTGIAV
LQFPDGLEWHPGQTTHLVFAIAAQSDEHITLLRRLTRLLNDDARLRQLFSTQRAEDIVAALSQDAPAPAASAPGGDLAER
LALTLNYPSGLHARPAAQWVETARRFAARVQVRHGAETADAKNLVALLQLGLAAGAALTLSAEGPDARAALTALQHTIRS
LTAQERAQAARDTQAARTRQIARGWQPAADLPAIGGIGASPGLAIGTVHVMQPGVSAIPDHPVPLATGGDLLDRALADTR
AELAALARDTAARLGEAEAGIFKAQAELLGDTDLMTLTCQAMVEGHGVAWSWHHAVERLAERLAALGNPLLAARAADLRD
VGRRVLGHLDPALRGTAQALPDGPCILVAQDLSPSDTAALDTRRIAGIVTAQGGPTSHTAILTRTLGIPAVVAAGPAVLD
VASGTQAIVDGSGGQLYLDPDAAAVAGAEAWLREDAARAQREQAERGLPARTRDGHAVEIAANVNLPAQAIEAVTLGAEG
VGLMRTEFLFLERDHAPDEDAQHEVYAAMLGALGGRPLIVRTLDIGGDKQVPHLNLPKEENPFLGVRGARLLLRRPDLME
PQLRALYRAASGGGPLSIMFPMITSLGEVIALRAACERIRAELDAPAVPLGIMVEVPAAALLADQLAEHVDFFSIGTNDL
TQYTLAIDRQHPDLAAEADSLHPAVLRLIQLTVQGAARHGRWVGVCGGLAGDPFGALLLTGLGVHELSMSPRDIPAVKAR
LRDAHVRQLTELAGQALACASAEDVRALEATLPAHRAGTEMAAA

Sequences:

>Translated_844_residues
MPSPLICAERIRLQQRATDKTSAIRAAGRLLADTGCIDPAYIDSLLRRETVANTFLGHGVAIPHGMGEDRHLIRQTGIAV
LQFPDGLEWHPGQTTHLVFAIAAQSDEHITLLRRLTRLLNDDARLRQLFSTQRAEDIVAALSQDAPAPAASAPGGDLAER
LALTLNYPSGLHARPAAQWVETARRFAARVQVRHGAETADAKNLVALLQLGLAAGAALTLSAEGPDARAALTALQHTIRS
LTAQERAQAARDTQAARTRQIARGWQPAADLPAIGGIGASPGLAIGTVHVMQPGVSAIPDHPVPLATGGDLLDRALADTR
AELAALARDTAARLGEAEAGIFKAQAELLGDTDLMTLTCQAMVEGHGVAWSWHHAVERLAERLAALGNPLLAARAADLRD
VGRRVLGHLDPALRGTAQALPDGPCILVAQDLSPSDTAALDTRRIAGIVTAQGGPTSHTAILTRTLGIPAVVAAGPAVLD
VASGTQAIVDGSGGQLYLDPDAAAVAGAEAWLREDAARAQREQAERGLPARTRDGHAVEIAANVNLPAQAIEAVTLGAEG
VGLMRTEFLFLERDHAPDEDAQHEVYAAMLGALGGRPLIVRTLDIGGDKQVPHLNLPKEENPFLGVRGARLLLRRPDLME
PQLRALYRAASGGGPLSIMFPMITSLGEVIALRAACERIRAELDAPAVPLGIMVEVPAAALLADQLAEHVDFFSIGTNDL
TQYTLAIDRQHPDLAAEADSLHPAVLRLIQLTVQGAARHGRWVGVCGGLAGDPFGALLLTGLGVHELSMSPRDIPAVKAR
LRDAHVRQLTELAGQALACASAEDVRALEATLPAHRAGTEMAAA
>Mature_843_residues
PSPLICAERIRLQQRATDKTSAIRAAGRLLADTGCIDPAYIDSLLRRETVANTFLGHGVAIPHGMGEDRHLIRQTGIAVL
QFPDGLEWHPGQTTHLVFAIAAQSDEHITLLRRLTRLLNDDARLRQLFSTQRAEDIVAALSQDAPAPAASAPGGDLAERL
ALTLNYPSGLHARPAAQWVETARRFAARVQVRHGAETADAKNLVALLQLGLAAGAALTLSAEGPDARAALTALQHTIRSL
TAQERAQAARDTQAARTRQIARGWQPAADLPAIGGIGASPGLAIGTVHVMQPGVSAIPDHPVPLATGGDLLDRALADTRA
ELAALARDTAARLGEAEAGIFKAQAELLGDTDLMTLTCQAMVEGHGVAWSWHHAVERLAERLAALGNPLLAARAADLRDV
GRRVLGHLDPALRGTAQALPDGPCILVAQDLSPSDTAALDTRRIAGIVTAQGGPTSHTAILTRTLGIPAVVAAGPAVLDV
ASGTQAIVDGSGGQLYLDPDAAAVAGAEAWLREDAARAQREQAERGLPARTRDGHAVEIAANVNLPAQAIEAVTLGAEGV
GLMRTEFLFLERDHAPDEDAQHEVYAAMLGALGGRPLIVRTLDIGGDKQVPHLNLPKEENPFLGVRGARLLLRRPDLMEP
QLRALYRAASGGGPLSIMFPMITSLGEVIALRAACERIRAELDAPAVPLGIMVEVPAAALLADQLAEHVDFFSIGTNDLT
QYTLAIDRQHPDLAAEADSLHPAVLRLIQLTVQGAARHGRWVGVCGGLAGDPFGALLLTGLGVHELSMSPRDIPAVKARL
RDAHVRQLTELAGQALACASAEDVRALEATLPAHRAGTEMAAA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=566, Percent_Identity=39.5759717314488, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788726, Length=680, Percent_Identity=35.4411764705882, Blast_Score=346, Evalue=3e-96,
Organism=Escherichia coli, GI48994992, Length=698, Percent_Identity=33.5243553008596, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1789193, Length=564, Percent_Identity=35.9929078014184, Blast_Score=306, Evalue=5e-84,
Organism=Escherichia coli, GI1787994, Length=415, Percent_Identity=28.9156626506024, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1788494, Length=127, Percent_Identity=43.3070866141732, Blast_Score=114, Evalue=4e-26,
Organism=Escherichia coli, GI1790027, Length=137, Percent_Identity=33.5766423357664, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI226510935, Length=324, Percent_Identity=27.4691358024691, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 88703; Mature: 88572

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00369 PTS_HPR_HIS ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPLICAERIRLQQRATDKTSAIRAAGRLLADTGCIDPAYIDSLLRRETVANTFLGHGV
CCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
AIPHGMGEDRHLIRQTGIAVLQFPDGLEWHPGQTTHLVFAIAAQSDEHITLLRRLTRLLN
CCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHC
DDARLRQLFSTQRAEDIVAALSQDAPAPAASAPGGDLAERLALTLNYPSGLHARPAAQWV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEEEEECCCCCCCCHHHHHH
ETARRFAARVQVRHGAETADAKNLVALLQLGLAAGAALTLSAEGPDARAALTALQHTIRS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
LTAQERAQAARDTQAARTRQIARGWQPAADLPAIGGIGASPGLAIGTVHVMQPGVSAIPD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
HPVPLATGGDLLDRALADTRAELAALARDTAARLGEAEAGIFKAQAELLGDTDLMTLTCQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
AMVEGHGVAWSWHHAVERLAERLAALGNPLLAARAADLRDVGRRVLGHLDPALRGTAQAL
HHHCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
PDGPCILVAQDLSPSDTAALDTRRIAGIVTAQGGPTSHTAILTRTLGIPAVVAAGPAVLD
CCCCEEEEECCCCCCCHHHHHHHHHEEEEEECCCCCCCHHHHHHHCCCCHHHHCCCCEEE
VASGTQAIVDGSGGQLYLDPDAAAVAGAEAWLREDAARAQREQAERGLPARTRDGHAVEI
ECCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
AANVNLPAQAIEAVTLGAEGVGLMRTEFLFLERDHAPDEDAQHEVYAAMLGALGGRPLIV
EECCCCCHHHHHHHHCCCCCCCHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEE
RTLDIGGDKQVPHLNLPKEENPFLGVRGARLLLRRPDLMEPQLRALYRAASGGGPLSIMF
EEEECCCCCCCCCCCCCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEHH
PMITSLGEVIALRAACERIRAELDAPAVPLGIMVEVPAAALLADQLAEHVDFFSIGTNDL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEECCCCHH
TQYTLAIDRQHPDLAAEADSLHPAVLRLIQLTVQGAARHGRWVGVCGGLAGDPFGALLLT
HEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
GLGVHELSMSPRDIPAVKARLRDAHVRQLTELAGQALACASAEDVRALEATLPAHRAGTE
CCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCHHHCCCH
MAAA
HCCC
>Mature Secondary Structure 
PSPLICAERIRLQQRATDKTSAIRAAGRLLADTGCIDPAYIDSLLRRETVANTFLGHGV
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
AIPHGMGEDRHLIRQTGIAVLQFPDGLEWHPGQTTHLVFAIAAQSDEHITLLRRLTRLLN
CCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHC
DDARLRQLFSTQRAEDIVAALSQDAPAPAASAPGGDLAERLALTLNYPSGLHARPAAQWV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEEEEECCCCCCCCHHHHHH
ETARRFAARVQVRHGAETADAKNLVALLQLGLAAGAALTLSAEGPDARAALTALQHTIRS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH
LTAQERAQAARDTQAARTRQIARGWQPAADLPAIGGIGASPGLAIGTVHVMQPGVSAIPD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
HPVPLATGGDLLDRALADTRAELAALARDTAARLGEAEAGIFKAQAELLGDTDLMTLTCQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
AMVEGHGVAWSWHHAVERLAERLAALGNPLLAARAADLRDVGRRVLGHLDPALRGTAQAL
HHHCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
PDGPCILVAQDLSPSDTAALDTRRIAGIVTAQGGPTSHTAILTRTLGIPAVVAAGPAVLD
CCCCEEEEECCCCCCCHHHHHHHHHEEEEEECCCCCCCHHHHHHHCCCCHHHHCCCCEEE
VASGTQAIVDGSGGQLYLDPDAAAVAGAEAWLREDAARAQREQAERGLPARTRDGHAVEI
ECCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
AANVNLPAQAIEAVTLGAEGVGLMRTEFLFLERDHAPDEDAQHEVYAAMLGALGGRPLIV
EECCCCCHHHHHHHHCCCCCCCHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEE
RTLDIGGDKQVPHLNLPKEENPFLGVRGARLLLRRPDLMEPQLRALYRAASGGGPLSIMF
EEEECCCCCCCCCCCCCCCCCCEEECCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEHH
PMITSLGEVIALRAACERIRAELDAPAVPLGIMVEVPAAALLADQLAEHVDFFSIGTNDL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHCCEEECCCCHH
TQYTLAIDRQHPDLAAEADSLHPAVLRLIQLTVQGAARHGRWVGVCGGLAGDPFGALLLT
HEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
GLGVHELSMSPRDIPAVKARLRDAHVRQLTELAGQALACASAEDVRALEATLPAHRAGTE
CCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCHHHCCCH
MAAA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496537; 12024217; 1655739 [H]