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The map label for this gene is ddl [H]

Identifier: 17547561

GI number: 17547561

Start: 3056943

End: 3057938

Strand: Reverse

Name: ddl [H]

Synonym: RSc2842

Alternate gene names: 17547561

Gene position: 3057938-3056943 (Counterclockwise)

Preceding gene: 17547562

Following gene: 17547560

Centisome position: 82.28

GC content: 70.08

Gene sequence:

>996_bases
ATGACGACCGGTCCGTTCGTCCCCCATCCGACGCTCGATCCGAAGTCCCTCGGCAAGGTGGGCGTGATCCTCGGCGGTCG
CTCGGCCGAGCGCGAGATCTCGCTGCTGTCGGGCAACGGCGTGCTGGCGGCGCTGCGCTCGCGCGGCGTCGACGCGCATC
CGTTCGACCCGGGCACGCAGCCGGTGGCCGGGCTCGCCGCGCAGGGCTTCCACCGCGTGGTGATCTCGCTGCACGGCCGC
TTCGGCGAGGACGGCACGATCCAGGGGCTGCTCGAGCAGTTCGGCATTCCCTACACCGGCAGCGGCGTACTCGCGTCTGC
GCTGGCGATGGACAAGGAAGCCACCAAGCGCTTGTGGCAGACGCACAGCCTGCCGACCCCCGATTTCGTGATGCTGCACG
CCGGCGCCGACTGGCAGGCCGTTGCCGACCGCCTGGGCCTGCCGCTGATCGTCAAGCCGGCGCGCGAGGGCTCGTCGATC
GGCCTGACCAAGGTGACCAGCGTGGCCGAATTGCCCGCCGCCTACGAAAAGGCCGCGCGCCTGGACCGCGACGTGATGGC
CGAACAGTTCATCGACGGCGACGAGCTGACCTGCGCCGTGATCGGCGAAGGCGAGCACGCGACGGCGCTGCCGCTGATCC
GCATCGTCGCGCCGCAGGCCAACTACGACTACCAGCACAAGTACTTCACCGACGACACGCGCTACGAGTGCCCCGCGCCG
ATTCCCGCCGAAGCGGCGGCGCGCGTGCAGGCGCTGGTGGTGCAGGCGTATCGCAGCCTGGGCTGCCGCGGCTGGGGCCG
CGCCGACATCATGCTGCGCAAGCACGATGGCGCGCCGTTCCTGCTCGAGATGAACACCTCGCCGGGCATGACCGGGCATT
CGCTGGTGCCGATGGCCGCGCGCGCGGTGGGCATCAGCTATGAAGATTTCGTGCTGCAGCTGGCGGCGACCGCGTCGCTT
GAGCTGCGCGCGAGCCACGACTGGAAACCGGAATAA

Upstream 100 bases:

>100_bases
TGGGTGCGGGATCGATCGGGCAGGTACCTGGTCAGGTGGTTGCGCGGCAGGCGGAAGTGCGTGCTGCGAACGTGGTCGAC
CTCAACGGGGGCGCCGCAGC

Downstream 100 bases:

>100_bases
GCGACAGGACGCCATCCGCCATGTGGAACAACACCCGCCTCCTCAACGTCATCGCGAGCGCGCTGTATGCGCTGTTCGCG
CTGGCGGCCGTGGGCGTGGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTTGPFVPHPTLDPKSLGKVGVILGGRSAEREISLLSGNGVLAALRSRGVDAHPFDPGTQPVAGLAAQGFHRVVISLHGR
FGEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRLWQTHSLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSI
GLTKVTSVAELPAAYEKAARLDRDVMAEQFIDGDELTCAVIGEGEHATALPLIRIVAPQANYDYQHKYFTDDTRYECPAP
IPAEAAARVQALVVQAYRSLGCRGWGRADIMLRKHDGAPFLLEMNTSPGMTGHSLVPMAARAVGISYEDFVLQLAATASL
ELRASHDWKPE

Sequences:

>Translated_331_residues
MTTGPFVPHPTLDPKSLGKVGVILGGRSAEREISLLSGNGVLAALRSRGVDAHPFDPGTQPVAGLAAQGFHRVVISLHGR
FGEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRLWQTHSLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSI
GLTKVTSVAELPAAYEKAARLDRDVMAEQFIDGDELTCAVIGEGEHATALPLIRIVAPQANYDYQHKYFTDDTRYECPAP
IPAEAAARVQALVVQAYRSLGCRGWGRADIMLRKHDGAPFLLEMNTSPGMTGHSLVPMAARAVGISYEDFVLQLAATASL
ELRASHDWKPE
>Mature_330_residues
TTGPFVPHPTLDPKSLGKVGVILGGRSAEREISLLSGNGVLAALRSRGVDAHPFDPGTQPVAGLAAQGFHRVVISLHGRF
GEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRLWQTHSLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSIG
LTKVTSVAELPAAYEKAARLDRDVMAEQFIDGDELTCAVIGEGEHATALPLIRIVAPQANYDYQHKYFTDDTRYECPAPI
PAEAAARVQALVVQAYRSLGCRGWGRADIMLRKHDGAPFLLEMNTSPGMTGHSLVPMAARAVGISYEDFVLQLAATASLE
LRASHDWKPE

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=50.4950495049505, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=33.6206896551724, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 35435; Mature: 35304

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGPFVPHPTLDPKSLGKVGVILGGRSAEREISLLSGNGVLAALRSRGVDAHPFDPGTQ
CCCCCCCCCCCCCHHHCCCEEEEECCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCC
PVAGLAAQGFHRVVISLHGRFGEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRLWQ
CHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHH
THSLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSIGLTKVTSVAELPAAYEKAAR
HCCCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LDRDVMAEQFIDGDELTCAVIGEGEHATALPLIRIVAPQANYDYQHKYFTDDTRYECPAP
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHEEEEECCCCCCCCCCEEECCCCCCCCCCC
IPAEAAARVQALVVQAYRSLGCRGWGRADIMLRKHDGAPFLLEMNTSPGMTGHSLVPMAA
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHH
RAVGISYEDFVLQLAATASLELRASHDWKPE
HHHCCCHHHHHHHHHHHCEEEEEECCCCCCC
>Mature Secondary Structure 
TTGPFVPHPTLDPKSLGKVGVILGGRSAEREISLLSGNGVLAALRSRGVDAHPFDPGTQ
CCCCCCCCCCCCHHHCCCEEEEECCCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCC
PVAGLAAQGFHRVVISLHGRFGEDGTIQGLLEQFGIPYTGSGVLASALAMDKEATKRLWQ
CHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHH
THSLPTPDFVMLHAGADWQAVADRLGLPLIVKPAREGSSIGLTKVTSVAELPAAYEKAAR
HCCCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
LDRDVMAEQFIDGDELTCAVIGEGEHATALPLIRIVAPQANYDYQHKYFTDDTRYECPAP
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHEEEEECCCCCCCCCCEEECCCCCCCCCCC
IPAEAAARVQALVVQAYRSLGCRGWGRADIMLRKHDGAPFLLEMNTSPGMTGHSLVPMAA
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHH
RAVGISYEDFVLQLAATASLELRASHDWKPE
HHHCCCHHHHHHHHHHHCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA