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The map label for this gene is ynfL [H]

Identifier: 17547307

GI number: 17547307

Start: 2794552

End: 2795496

Strand: Reverse

Name: ynfL [H]

Synonym: RSc2588

Alternate gene names: 17547307

Gene position: 2795496-2794552 (Counterclockwise)

Preceding gene: 17547308

Following gene: 17547306

Centisome position: 75.22

GC content: 63.49

Gene sequence:

>945_bases
ATGGAGATTAGGCATCTGCGTTGTTTTCTTGCGGTCGCCGAAGAACTCCACTTCGCCCGCGCAGCGGAGAAGCTGCATAT
CGAGCAATCGCCGCTGTCGCGCACCATCAAAGAACTGGAAGAAGACCTAGGCGAACAGTTGTTCATCCGCACCAGCCGCA
GCACACGACTGACGCGGGCGGGAAAACTGTTCCTTGAGCATGTGCCACGTGTGTTCACTGCCTTGCAACAAGCGCGCGAC
AGCGTGAGGGCCGCAAGCAATGGATTCCACGGTCAGTTGCGCATCGCACTGTCCGACGGCATCACGCCGTCGCGCCTGCC
AGCTTTGCTGGCGATGTGCAGGCAGGAGGAACCCGAGGTCGATATCCGTCTGTTCGAGGTGCCGCTGTCGCAACAGATCA
AGGGACTGCATGACGACCTGTACGACGTGGGTTTCGCCCAGTCCGATGAGGTAGGCGAATGCATCGCAGCCGAAGCGGTG
TGGAGCGATCCGCTGATGGTGGCCGTGCCAGCGCGCCATCCCCTGCTCAAGCACAAGCGCATTCCCTTGGAAGAAATGCT
GCGTTACCCGCTGGTGCTGTGCGATCCACTGGCGTGCGAAGGGCATGCACGCCAGGTAGAGCGGGTGCTGCGCCGCTCGG
ACATGGAGCCGCTGATTGCCGAGCGCGTGGCCTCCTGCGATTTGATGATGGCGCTCGTGTCAGCAGGCTTCGCGCTGGGG
CTGACCGGCGCACCCCACATCGTGACCAGCCGCGAGCCCGGTGTGGTCGCGCGACCCTTGGCGGGCCGCTCCCCCGTGCT
GACAACCTATCTGTTGCATCGGGAGGGCGAGTCTTCGGCAGTGCTGTCCCGGTTCATCGAACGGGTGCAGGCCATCGACT
TGCCCGAAGGCGCACGACCCGCGCTGCCATCTGAAGCCGCGCCCCCGGAGGACACCGAGCCATGA

Upstream 100 bases:

>100_bases
TAACCCAAGGCCTCTCAAATTTGCTTTGAAATAAGTAGGCTCGCATTGATGTCTGGCAATGGTCAGAAGTGAGCCTCGTG
AGAAATAGGAGAAGCAGGCG

Downstream 100 bases:

>100_bases
AGCGAATGATCCCCTTGCTGCTGGTCGCAGCACTTGCGGCCTGCGGCCCATCGGAAACGCCCAGGCAGGCCAACGTGCCG
ACCGTGGAAGAACTTGCCGC

Product: transcription regulator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MEIRHLRCFLAVAEELHFARAAEKLHIEQSPLSRTIKELEEDLGEQLFIRTSRSTRLTRAGKLFLEHVPRVFTALQQARD
SVRAASNGFHGQLRIALSDGITPSRLPALLAMCRQEEPEVDIRLFEVPLSQQIKGLHDDLYDVGFAQSDEVGECIAAEAV
WSDPLMVAVPARHPLLKHKRIPLEEMLRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
LTGAPHIVTSREPGVVARPLAGRSPVLTTYLLHREGESSAVLSRFIERVQAIDLPEGARPALPSEAAPPEDTEP

Sequences:

>Translated_314_residues
MEIRHLRCFLAVAEELHFARAAEKLHIEQSPLSRTIKELEEDLGEQLFIRTSRSTRLTRAGKLFLEHVPRVFTALQQARD
SVRAASNGFHGQLRIALSDGITPSRLPALLAMCRQEEPEVDIRLFEVPLSQQIKGLHDDLYDVGFAQSDEVGECIAAEAV
WSDPLMVAVPARHPLLKHKRIPLEEMLRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
LTGAPHIVTSREPGVVARPLAGRSPVLTTYLLHREGESSAVLSRFIERVQAIDLPEGARPALPSEAAPPEDTEP
>Mature_314_residues
MEIRHLRCFLAVAEELHFARAAEKLHIEQSPLSRTIKELEEDLGEQLFIRTSRSTRLTRAGKLFLEHVPRVFTALQQARD
SVRAASNGFHGQLRIALSDGITPSRLPALLAMCRQEEPEVDIRLFEVPLSQQIKGLHDDLYDVGFAQSDEVGECIAAEAV
WSDPLMVAVPARHPLLKHKRIPLEEMLRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
LTGAPHIVTSREPGVVARPLAGRSPVLTTYLLHREGESSAVLSRFIERVQAIDLPEGARPALPSEAAPPEDTEP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=242, Percent_Identity=33.4710743801653, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=31.9587628865979, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=31.6981132075472, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI157672245, Length=234, Percent_Identity=29.9145299145299, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790399, Length=295, Percent_Identity=26.4406779661017, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=27.734375, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787806, Length=303, Percent_Identity=26.7326732673267, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787601, Length=142, Percent_Identity=32.3943661971831, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=29.2307692307692, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693105, Length=296, Percent_Identity=25.6756756756757, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788748, Length=269, Percent_Identity=25.6505576208178, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788297, Length=127, Percent_Identity=34.6456692913386, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786713, Length=243, Percent_Identity=30.8641975308642, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI48994962, Length=146, Percent_Identity=32.1917808219178, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787949, Length=132, Percent_Identity=32.5757575757576, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=144, Percent_Identity=30.5555555555556, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788296, Length=243, Percent_Identity=26.3374485596708, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=179, Percent_Identity=31.2849162011173, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=34.1463414634146, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34810; Mature: 34810

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRHLRCFLAVAEELHFARAAEKLHIEQSPLSRTIKELEEDLGEQLFIRTSRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHEEEECCCCHHHHHH
GKLFLEHVPRVFTALQQARDSVRAASNGFHGQLRIALSDGITPSRLPALLAMCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCC
DIRLFEVPLSQQIKGLHDDLYDVGFAQSDEVGECIAAEAVWSDPLMVAVPARHPLLKHKR
CEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHCC
IPLEEMLRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
CCHHHHHHCCHHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHCC
LTGAPHIVTSREPGVVARPLAGRSPVLTTYLLHREGESSAVLSRFIERVQAIDLPEGARP
CCCCCCEEECCCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
ALPSEAAPPEDTEP
CCCCCCCCCCCCCC
>Mature Secondary Structure
MEIRHLRCFLAVAEELHFARAAEKLHIEQSPLSRTIKELEEDLGEQLFIRTSRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHEEEECCCCHHHHHH
GKLFLEHVPRVFTALQQARDSVRAASNGFHGQLRIALSDGITPSRLPALLAMCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCC
DIRLFEVPLSQQIKGLHDDLYDVGFAQSDEVGECIAAEAVWSDPLMVAVPARHPLLKHKR
CEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHCC
IPLEEMLRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
CCHHHHHHCCHHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHCC
LTGAPHIVTSREPGVVARPLAGRSPVLTTYLLHREGESSAVLSRFIERVQAIDLPEGARP
CCCCCCEEECCCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
ALPSEAAPPEDTEP
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]