Click here to switch to the map view.

The map label for this gene is xerD

Identifier: 17547263

GI number: 17547263

Start: 2752033

End: 2752959

Strand: Direct

Name: xerD

Synonym: RSc2544

Alternate gene names: 17547263

Gene position: 2752033-2752959 (Clockwise)

Preceding gene: 17547262

Following gene: 17547264

Centisome position: 74.05

GC content: 71.74

Gene sequence:

>927_bases
ATGACCCAGGCCGCCCCCGAAACCCTGCCCCTGCCCCCGAGCAGCACGGAGGCGATCCAGCGCTTCTGCGACGCGCTGTG
GCTCGAGGACGGCCTCGCCCGCAACACGCTCGATGCCTACCGGCGCGACCTGACGCTCTACGCGCAGTGGCTCGCCGGGC
GCGGCAAGGCGCTCGACCAGACCGAGGACGTCGACCTCTCCGACTACTTCGCCGCCCGCCACGAAGACAGCCTCGCCTCC
ACCGCCAACCGGCGGCGCACGGTGTTCAAGCGCTTCTTCCAATGGGCCTTGCGCGAGCACCTGGTCAGCGCCGACCCGAC
GCGCCTGCTGTCGACCGCCAAGCAGCCGCCGCGCGTTCCCAAGACGCTGTCCGAGGCGCAGGTCGAGGCGCTGATCGCCG
CGCCCGATGTCGACGCCCCGCTCGGCCTGCGCGACCGCGCCATGATCGAGCTGATGTATGCGAGCGGCCTGCGCGTGTCG
GAGATCGTCGCGCTCAAGACCGTCGAGGTCGGCCTGAACGAGGGTGTGGTGCGCGTGATCGGCGGCAAGGGCGGCAAGGA
CCGGCTGGTGCCGTTCGGCGCCGAGGCCGGCGACTGGCTGCGGCGCTACCTGCGCGACGGCCGCACCGCGCTGCTGGGCG
AGCGCACCGCCGATGCGCTGTTCGTCACCGCGCGCGGCGACGGCATGACGCGCCAGGCCTTCTGGTACCTGATCAAGCGC
TATGCCCAGCGCGCCGACATCCACGCGCCGCTGTCGCCGCACACGCTGCGGCACGCCTTCGCCACGCACCTGCTCAACCA
CGGCGCCGACCTGCGCGTGGTGCAGATGCTGCTCGGCCACGCCGACATCTCCACCACGCAGATCTACACCCATGTCGCGC
GCGAGCGGCTGCGCACCCTGCATGCACAGCACCATCCGCGCGGATAG

Upstream 100 bases:

>100_bases
TGAGCGCCTCGGGCATCGGCGGCTTCTCCAACCACGATGAGGACGGGTTCCACCTGAACGTCAAGCGCTGGCTGCTGCGC
CACGAAGGCGTGATGCTGGC

Downstream 100 bases:

>100_bases
GCTTTCCGCATGAGCAAACCCCGCCACGTTTCCGAGACGCCCGCCACGCAGTTCCTGAAGGCGCACGGCATCGCCTACAC
CGAACACGCCTACGACTACG

Product: site-specific tyrosine recombinase XerD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTQAAPETLPLPPSSTEAIQRFCDALWLEDGLARNTLDAYRRDLTLYAQWLAGRGKALDQTEDVDLSDYFAARHEDSLAS
TANRRRTVFKRFFQWALREHLVSADPTRLLSTAKQPPRVPKTLSEAQVEALIAAPDVDAPLGLRDRAMIELMYASGLRVS
EIVALKTVEVGLNEGVVRVIGGKGGKDRLVPFGAEAGDWLRRYLRDGRTALLGERTADALFVTARGDGMTRQAFWYLIKR
YAQRADIHAPLSPHTLRHAFATHLLNHGADLRVVQMLLGHADISTTQIYTHVARERLRTLHAQHHPRG

Sequences:

>Translated_308_residues
MTQAAPETLPLPPSSTEAIQRFCDALWLEDGLARNTLDAYRRDLTLYAQWLAGRGKALDQTEDVDLSDYFAARHEDSLAS
TANRRRTVFKRFFQWALREHLVSADPTRLLSTAKQPPRVPKTLSEAQVEALIAAPDVDAPLGLRDRAMIELMYASGLRVS
EIVALKTVEVGLNEGVVRVIGGKGGKDRLVPFGAEAGDWLRRYLRDGRTALLGERTADALFVTARGDGMTRQAFWYLIKR
YAQRADIHAPLSPHTLRHAFATHLLNHGADLRVVQMLLGHADISTTQIYTHVARERLRTLHAQHHPRG
>Mature_307_residues
TQAAPETLPLPPSSTEAIQRFCDALWLEDGLARNTLDAYRRDLTLYAQWLAGRGKALDQTEDVDLSDYFAARHEDSLAST
ANRRRTVFKRFFQWALREHLVSADPTRLLSTAKQPPRVPKTLSEAQVEALIAAPDVDAPLGLRDRAMIELMYASGLRVSE
IVALKTVEVGLNEGVVRVIGGKGGKDRLVPFGAEAGDWLRRYLRDGRTALLGERTADALFVTARGDGMTRQAFWYLIKRY
AQRADIHAPLSPHTLRHAFATHLLNHGADLRVVQMLLGHADISTTQIYTHVARERLRTLHAQHHPRG

Specific function: Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The xerC-xerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell div

COG id: COG4974

COG function: function code L; Site-specific recombinase XerD

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family. XerD subfamily

Homologues:

Organism=Escherichia coli, GI1789261, Length=292, Percent_Identity=56.8493150684932, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1790244, Length=293, Percent_Identity=34.8122866894198, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1790768, Length=170, Percent_Identity=31.7647058823529, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790767, Length=168, Percent_Identity=26.7857142857143, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): XERD_RALSO (Q8XWD0)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520665.1
- ProteinModelPortal:   Q8XWD0
- SMR:   Q8XWD0
- GeneID:   1221391
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2544
- NMPDR:   fig|267608.1.peg.2544
- HOGENOM:   HBG727654
- OMA:   FGEEAHG
- ProtClustDB:   PRK00283
- BioCyc:   RSOL267608:RSC2544-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01807
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR010998
- InterPro:   IPR023109
- InterPro:   IPR004107
- InterPro:   IPR011932
- Gene3D:   G3DSA:1.10.150.130
- Gene3D:   G3DSA:1.10.443.10
- TIGRFAMs:   TIGR02225

Pfam domain/function: PF02899 Phage_integr_N; PF00589 Phage_integrase; SSF56349 DNA_brk_join_enz; SSF47823 L_intgrse_like_N

EC number: NA

Molecular weight: Translated: 34454; Mature: 34323

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: ACT_SITE 158-158 ACT_SITE 183-183 ACT_SITE 254-254 ACT_SITE 257-257 ACT_SITE 280-280 ACT_SITE 289-289

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAAPETLPLPPSSTEAIQRFCDALWLEDGLARNTLDAYRRDLTLYAQWLAGRGKALDQ
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEDVDLSDYFAARHEDSLASTANRRRTVFKRFFQWALREHLVSADPTRLLSTAKQPPRVP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
KTLSEAQVEALIAAPDVDAPLGLRDRAMIELMYASGLRVSEIVALKTVEVGLNEGVVRVI
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEE
GGKGGKDRLVPFGAEAGDWLRRYLRDGRTALLGERTADALFVTARGDGMTRQAFWYLIKR
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHH
YAQRADIHAPLSPHTLRHAFATHLLNHGADLRVVQMLLGHADISTTQIYTHVARERLRTL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HAQHHPRG
HHHCCCCC
>Mature Secondary Structure 
TQAAPETLPLPPSSTEAIQRFCDALWLEDGLARNTLDAYRRDLTLYAQWLAGRGKALDQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEDVDLSDYFAARHEDSLASTANRRRTVFKRFFQWALREHLVSADPTRLLSTAKQPPRVP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
KTLSEAQVEALIAAPDVDAPLGLRDRAMIELMYASGLRVSEIVALKTVEVGLNEGVVRVI
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEE
GGKGGKDRLVPFGAEAGDWLRRYLRDGRTALLGERTADALFVTARGDGMTRQAFWYLIKR
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCHHHHHHHHHHH
YAQRADIHAPLSPHTLRHAFATHLLNHGADLRVVQMLLGHADISTTQIYTHVARERLRTL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
HAQHHPRG
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852