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The map label for this gene is ogt [H]

Identifier: 17547262

GI number: 17547262

Start: 2751515

End: 2752036

Strand: Direct

Name: ogt [H]

Synonym: RSc2543

Alternate gene names: 17547262

Gene position: 2751515-2752036 (Clockwise)

Preceding gene: 17547261

Following gene: 17547263

Centisome position: 74.04

GC content: 68.97

Gene sequence:

>522_bases
GTGCTAGATTCGCAGGATCGCTTTCCCGTGCCGCACACCGCCATGCCATCCCCGTTCGACGCCGTTCTGGAAGCGCCCTT
CGGCAAGCTGGGCGTGCGCGTGGTCGAGGGCGCCCTGTGGGAGATCGTCTACCTGCCTGACAGCGCGCGCACCGTCGACT
CGGCGGCCCCGTTGATCCGCCGGCTGGCGCATCAGCTCGATGCCTACTACGACAATGCCGATGCCGTGTTCGACGTGCCG
CTCACGCACACCGGCACCGGCTTCCAGCACCGCGTGTGGGACGCCATCTGCGCGGTGCCGCGCGGCGCCGTCACCACCTA
CGGCACCCTCGCCAAGCAGATCCGCAGCGCACCGCGCGCCGTGGGCCAGGCGTGCGGCGCGAACTGGTATCCGATCCTCA
TCCCCTGCCACCGCGTGGTGAGCGCCTCGGGCATCGGCGGCTTCTCCAACCACGATGAGGACGGGTTCCACCTGAACGTC
AAGCGCTGGCTGCTGCGCCACGAAGGCGTGATGCTGGCATGA

Upstream 100 bases:

>100_bases
ATCGGCGACGACCCGGTCGACCGCGACCTCAAGAGCGGACTGCTCGGCACCTGAGCGCGGGCCGCGCGGCCGGGGCGGTG
CCCGCCCGGAGACCGCGCCT

Downstream 100 bases:

>100_bases
CCCAGGCCGCCCCCGAAACCCTGCCCCTGCCCCCGAGCAGCACGGAGGCGATCCAGCGCTTCTGCGACGCGCTGTGGCTC
GAGGACGGCCTCGCCCGCAA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MLDSQDRFPVPHTAMPSPFDAVLEAPFGKLGVRVVEGALWEIVYLPDSARTVDSAAPLIRRLAHQLDAYYDNADAVFDVP
LTHTGTGFQHRVWDAICAVPRGAVTTYGTLAKQIRSAPRAVGQACGANWYPILIPCHRVVSASGIGGFSNHDEDGFHLNV
KRWLLRHEGVMLA

Sequences:

>Translated_173_residues
MLDSQDRFPVPHTAMPSPFDAVLEAPFGKLGVRVVEGALWEIVYLPDSARTVDSAAPLIRRLAHQLDAYYDNADAVFDVP
LTHTGTGFQHRVWDAICAVPRGAVTTYGTLAKQIRSAPRAVGQACGANWYPILIPCHRVVSASGIGGFSNHDEDGFHLNV
KRWLLRHEGVMLA
>Mature_173_residues
MLDSQDRFPVPHTAMPSPFDAVLEAPFGKLGVRVVEGALWEIVYLPDSARTVDSAAPLIRRLAHQLDAYYDNADAVFDVP
LTHTGTGFQHRVWDAICAVPRGAVTTYGTLAKQIRSAPRAVGQACGANWYPILIPCHRVVSASGIGGFSNHDEDGFHLNV
KRWLLRHEGVMLA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=170, Percent_Identity=36.4705882352941, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787596, Length=115, Percent_Identity=38.2608695652174, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788542, Length=105, Percent_Identity=38.0952380952381, Blast_Score=64, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=61, Percent_Identity=47.5409836065574, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18929; Mature: 18929

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSQDRFPVPHTAMPSPFDAVLEAPFGKLGVRVVEGALWEIVYLPDSARTVDSAAPLIR
CCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHH
RLAHQLDAYYDNADAVFDVPLTHTGTGFQHRVWDAICAVPRGAVTTYGTLAKQIRSAPRA
HHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VGQACGANWYPILIPCHRVVSASGIGGFSNHDEDGFHLNVKRWLLRHEGVMLA
HHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCEECC
>Mature Secondary Structure
MLDSQDRFPVPHTAMPSPFDAVLEAPFGKLGVRVVEGALWEIVYLPDSARTVDSAAPLIR
CCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHH
RLAHQLDAYYDNADAVFDVPLTHTGTGFQHRVWDAICAVPRGAVTTYGTLAKQIRSAPRA
HHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
VGQACGANWYPILIPCHRVVSASGIGGFSNHDEDGFHLNVKRWLLRHEGVMLA
HHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]