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The map label for this gene is amiC [H]

Identifier: 17547258

GI number: 17547258

Start: 2747322

End: 2748845

Strand: Reverse

Name: amiC [H]

Synonym: RSc2539

Alternate gene names: 17547258

Gene position: 2748845-2747322 (Counterclockwise)

Preceding gene: 17547259

Following gene: 17547256

Centisome position: 73.97

GC content: 70.54

Gene sequence:

>1524_bases
ATGCTGATCAAGCATCTGCCGACCGACCAACCGGACGATTCCCACCTGCCCAGCCAGGCGCGCAGGCAATGGCTGTCGCG
CATGGCCCGGGCGGGCGCCGGGACGGTCGTCCTCAGCCTGGCCGGTCCGCAGATCGCCTTCGGCGCCAACATCGTGGCGG
TGCGCGTGTGGCCGGCGCAGGATTACACGCGCGTGACCATCGAGTCGGACATGGCGCTGTCGGCCACGCACCAGCTGGTG
GCGGACCCGGACCGCCTGGTTGTCGACATCGACGGCCTTGACCTGAACCCGACGCTGCGCGAGCTGGTGGCCAAGATCAC
GCCGAACGATCCGTACATCCGCCAGGTGCGCGTGGGCCAGAACCGGCCGCGCGTGGTGCGCCTGGTGTTCGACCTGAAGG
AGAGCGTCAATCCGCAGGTGTTCACGCTGCTGCCGATCGCCGGCTATCGCAACCGGCTGGTGTTCGACCTGTATCCGGCC
AATCCGCTTGACCCGTTGATGCAGCTGGTGCGCGCGACCGAAGACAAGCAGCAGCGCTTCATCGCCTCGGGCGGCGCGCC
GATGCCGGCACCGGCCGAGGACGACGATCCCATCGCCGCGCTGGCGCGCCGCAGCGCCGGCAGCCCGGCGTCCGCGGCCA
CGCGGCCGGCCGCGGATGGGCGTCCGGCGTTGGCCAATCGCGCGCCGTCGCGCAAGCCCGCGTCGCCCGCCGCCGACAAT
CCGCCGGCGCTGGCCAACGCGCCGATGTCGCCGCCGCTGGCGATCGAACCCGAGCCGCCGGTGCCGCGCGGCGCGGCTGC
CGGTCCGCGCATGCGCCGGCTGCTGACGGTGGCGATCGATCCCGGCCACGGCGGCGAAGACCCGGGCGCCACCGGCAGCG
CCGGCACGCACGAGAAGGACGTGGTGCTGTCGGTCGCCCGCCTGCTGCGCGCCAAGATCGACGCCCAGCCCAACATGCGC
GCCATGATGACGCGCGACGCCGACTACTTCGTGCCGCTCAACGTGCGCGTGCAGAAGGCGCGCCGCGTGCAGGCCGACCT
GTTCGTCTCCATCCACGCCGATGCCTTCCTGTCGCCGGAGGCGAAGGGGGCATCGGTGTTTGCGCTGTCCGAGCGCGGCG
CCTCCAGCGCCCAGGCCAAGTGGCTCGCCAACAAGGAGAACGCGGCGGACATGATCGGCGGGGCCAACCTGGGCTCCAAG
GATGCGCAGGTCACGCGCGTGCTGCTGGACCTGTCGACCACCGCGCAGATCAGCGATAGCATGAAGGTCGGCCGGTCGGT
GCTGGAGCAGATCGGCGGCATCAACCGCCTGCACAAGGGACAGGTCGAGCAGGCCGGGTTTGCGGTGCTGAAGGCGCCGG
ACATTCCGTCGATCCTGATCGAGACGGCGTTCATCAGCAACCCGGAAGAAGAGTCCAAGCTCACCGACAGCGCCTACCAG
AATCAGCTGGCCGAGGCGATCCTGCGCGGCATCCGCGCCTACTTCGCCAAGAACCCGCCGCTGTCGCGCAATCCGGGCGT
GTGA

Upstream 100 bases:

>100_bases
TGCACGAAACCTACGACGACGGCGTTGAACACGCACCACGCCTCGCGCGCCTGAGCGCACGCACGCCGACCGGCCTTCAA
CTGTTGCAACTGCTGCCCCC

Downstream 100 bases:

>100_bases
CGGCCGGCGGCGATGCTGTCCTGAGCGCGCCGGCGTAGATCGTGGTGTGGCCGATCAGGCGGGCTGGCCGGCGCGGTCGG
CGGCCCGGTCTTCCGCGTGC

Product: N-acetylmuramoyl-L-alanine amidase AMIC precursor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLIKHLPTDQPDDSHLPSQARRQWLSRMARAGAGTVVLSLAGPQIAFGANIVAVRVWPAQDYTRVTIESDMALSATHQLV
ADPDRLVVDIDGLDLNPTLRELVAKITPNDPYIRQVRVGQNRPRVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPA
NPLDPLMQLVRATEDKQQRFIASGGAPMPAPAEDDDPIAALARRSAGSPASAATRPAADGRPALANRAPSRKPASPAADN
PPALANAPMSPPLAIEPEPPVPRGAAAGPRMRRLLTVAIDPGHGGEDPGATGSAGTHEKDVVLSVARLLRAKIDAQPNMR
AMMTRDADYFVPLNVRVQKARRVQADLFVSIHADAFLSPEAKGASVFALSERGASSAQAKWLANKENAADMIGGANLGSK
DAQVTRVLLDLSTTAQISDSMKVGRSVLEQIGGINRLHKGQVEQAGFAVLKAPDIPSILIETAFISNPEEESKLTDSAYQ
NQLAEAILRGIRAYFAKNPPLSRNPGV

Sequences:

>Translated_507_residues
MLIKHLPTDQPDDSHLPSQARRQWLSRMARAGAGTVVLSLAGPQIAFGANIVAVRVWPAQDYTRVTIESDMALSATHQLV
ADPDRLVVDIDGLDLNPTLRELVAKITPNDPYIRQVRVGQNRPRVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPA
NPLDPLMQLVRATEDKQQRFIASGGAPMPAPAEDDDPIAALARRSAGSPASAATRPAADGRPALANRAPSRKPASPAADN
PPALANAPMSPPLAIEPEPPVPRGAAAGPRMRRLLTVAIDPGHGGEDPGATGSAGTHEKDVVLSVARLLRAKIDAQPNMR
AMMTRDADYFVPLNVRVQKARRVQADLFVSIHADAFLSPEAKGASVFALSERGASSAQAKWLANKENAADMIGGANLGSK
DAQVTRVLLDLSTTAQISDSMKVGRSVLEQIGGINRLHKGQVEQAGFAVLKAPDIPSILIETAFISNPEEESKLTDSAYQ
NQLAEAILRGIRAYFAKNPPLSRNPGV
>Mature_507_residues
MLIKHLPTDQPDDSHLPSQARRQWLSRMARAGAGTVVLSLAGPQIAFGANIVAVRVWPAQDYTRVTIESDMALSATHQLV
ADPDRLVVDIDGLDLNPTLRELVAKITPNDPYIRQVRVGQNRPRVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPA
NPLDPLMQLVRATEDKQQRFIASGGAPMPAPAEDDDPIAALARRSAGSPASAATRPAADGRPALANRAPSRKPASPAADN
PPALANAPMSPPLAIEPEPPVPRGAAAGPRMRRLLTVAIDPGHGGEDPGATGSAGTHEKDVVLSVARLLRAKIDAQPNMR
AMMTRDADYFVPLNVRVQKARRVQADLFVSIHADAFLSPEAKGASVFALSERGASSAQAKWLANKENAADMIGGANLGSK
DAQVTRVLLDLSTTAQISDSMKVGRSVLEQIGGINRLHKGQVEQAGFAVLKAPDIPSILIETAFISNPEEESKLTDSAYQ
NQLAEAILRGIRAYFAKNPPLSRNPGV

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=246, Percent_Identity=55.6910569105691, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI1788776, Length=219, Percent_Identity=48.4018264840183, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1790611, Length=233, Percent_Identity=43.3476394849785, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 54288; Mature: 54288

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIKHLPTDQPDDSHLPSQARRQWLSRMARAGAGTVVLSLAGPQIAFGANIVAVRVWPAQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEEECCC
DYTRVTIESDMALSATHQLVADPDRLVVDIDGLDLNPTLRELVAKITPNDPYIRQVRVGQ
CCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCC
NRPRVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPANPLDPLMQLVRATEDKQQRF
CCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHH
IASGGAPMPAPAEDDDPIAALARRSAGSPASAATRPAADGRPALANRAPSRKPASPAADN
HHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
PPALANAPMSPPLAIEPEPPVPRGAAAGPRMRRLLTVAIDPGHGGEDPGATGSAGTHEKD
CCCCCCCCCCCCEEECCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCCCCHHH
VVLSVARLLRAKIDAQPNMRAMMTRDADYFVPLNVRVQKARRVQADLFVSIHADAFLSPE
HHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCEEHHHHHHCCEEEEEEEECCEEECCC
AKGASVFALSERGASSAQAKWLANKENAADMIGGANLGSKDAQVTRVLLDLSTTAQISDS
CCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHH
MKVGRSVLEQIGGINRLHKGQVEQAGFAVLKAPDIPSILIETAFISNPEEESKLTDSAYQ
HHHHHHHHHHHCCCCHHHCCCHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHH
NQLAEAILRGIRAYFAKNPPLSRNPGV
HHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLIKHLPTDQPDDSHLPSQARRQWLSRMARAGAGTVVLSLAGPQIAFGANIVAVRVWPAQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEECCCEEEEEEECCC
DYTRVTIESDMALSATHQLVADPDRLVVDIDGLDLNPTLRELVAKITPNDPYIRQVRVGQ
CCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEECCC
NRPRVVRLVFDLKESVNPQVFTLLPIAGYRNRLVFDLYPANPLDPLMQLVRATEDKQQRF
CCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCHHHHHHH
IASGGAPMPAPAEDDDPIAALARRSAGSPASAATRPAADGRPALANRAPSRKPASPAADN
HHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
PPALANAPMSPPLAIEPEPPVPRGAAAGPRMRRLLTVAIDPGHGGEDPGATGSAGTHEKD
CCCCCCCCCCCCEEECCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCCCCHHH
VVLSVARLLRAKIDAQPNMRAMMTRDADYFVPLNVRVQKARRVQADLFVSIHADAFLSPE
HHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCEEHHHHHHCCEEEEEEEECCEEECCC
AKGASVFALSERGASSAQAKWLANKENAADMIGGANLGSKDAQVTRVLLDLSTTAQISDS
CCCCEEEEEECCCCCCHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHH
MKVGRSVLEQIGGINRLHKGQVEQAGFAVLKAPDIPSILIETAFISNPEEESKLTDSAYQ
HHHHHHHHHHHCCCCHHHCCCHHHCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHH
NQLAEAILRGIRAYFAKNPPLSRNPGV
HHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]