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The map label for this gene is fabG2 [H]

Identifier: 17547253

GI number: 17547253

Start: 2741423

End: 2742181

Strand: Direct

Name: fabG2 [H]

Synonym: RSc2534

Alternate gene names: 17547253

Gene position: 2741423-2742181 (Clockwise)

Preceding gene: 17547252

Following gene: 17547255

Centisome position: 73.77

GC content: 71.41

Gene sequence:

>759_bases
ATGGAGATTCGCGATCAGGTGTTCATCGTCACCGGCGGCGCGTCGGGCTTGGGCGCGGGAACGACGCGCGCGCTGGCGGA
GGCCGGCGGCAAGGTGGTGATCGCAGACCTGAACGAGGCCGCCGGCGCGGCGCTGGCGCAGGAGATCGGCGGGCGCTTCG
TGCGCTGCGACGTCAGCTCGGAAGCCGACGGCCAGGCCGCGGTGCAGGCCGCCACCTCGCTCGGCGCGCTGGCGGGGCTG
GTGAACTGCGCGGGCATCGCCCCGGCGTCGCGCACGGTGGGCAAGGCCGGACCGCACCCGCTCGACCAGTTCGCGCGCGT
CATCAACATCAACCTGATCGGCACGTTCAACATGATCCGCCTGGCGGCCACGGCGATGACCGCCAACGCCCCCAATGCCG
GCGGCGAGCGCGGCGTCATCATCAACACCGCGTCGGTGGCGGCGTTCGACGGGCAGATCGGCCAGGCCGCCTATGCGGCA
TCCAAGGGCGGCGTGGTGGCGATGACGCTGGCGATCGCGCGCGACCTGTCGCGCGACGGCATCCGCGTGATGACGATCGC
GCCGGGCATCTTCGAGACGCCGATGCTGCTGGGCATGCCGCCGGAGGTGCAGGATGCGCTGGGCAAGATGGTGCCGTTCC
CGCCGCGGCTGGGGAAACCGGCGGAGTATGCGCAGCTGGCGCGCGCCATCATCGAGAACCCGATGCTCAACGGCGAGACG
ATCCGGCTCGACGGCGCCATCCGCATGCAGCCGAAGTAA

Upstream 100 bases:

>100_bases
CGCGCCGACCGCCCGGCCTGCCGTCCACCGCCGGCTTCGCGGTAACATATGACGTTGACGTAAACGTCAATTACCTCGAA
GACCCCATCAGGAGACAGGC

Downstream 100 bases:

>100_bases
GGCCGACTGGCTGTGAGCGTCAGGCGGTGGTTTATGGACGGGCGCGCACCGGCCGCCAGGCGGCTGATGTATCGTCGGGA
AGATCACCCTGGATGGACTC

Product: 3-hydroxyacyl-CoA dehydrogenase type II oxidoreductase

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MEIRDQVFIVTGGASGLGAGTTRALAEAGGKVVIADLNEAAGAALAQEIGGRFVRCDVSSEADGQAAVQAATSLGALAGL
VNCAGIAPASRTVGKAGPHPLDQFARVININLIGTFNMIRLAATAMTANAPNAGGERGVIINTASVAAFDGQIGQAAYAA
SKGGVVAMTLAIARDLSRDGIRVMTIAPGIFETPMLLGMPPEVQDALGKMVPFPPRLGKPAEYAQLARAIIENPMLNGET
IRLDGAIRMQPK

Sequences:

>Translated_252_residues
MEIRDQVFIVTGGASGLGAGTTRALAEAGGKVVIADLNEAAGAALAQEIGGRFVRCDVSSEADGQAAVQAATSLGALAGL
VNCAGIAPASRTVGKAGPHPLDQFARVININLIGTFNMIRLAATAMTANAPNAGGERGVIINTASVAAFDGQIGQAAYAA
SKGGVVAMTLAIARDLSRDGIRVMTIAPGIFETPMLLGMPPEVQDALGKMVPFPPRLGKPAEYAQLARAIIENPMLNGET
IRLDGAIRMQPK
>Mature_252_residues
MEIRDQVFIVTGGASGLGAGTTRALAEAGGKVVIADLNEAAGAALAQEIGGRFVRCDVSSEADGQAAVQAATSLGALAGL
VNCAGIAPASRTVGKAGPHPLDQFARVININLIGTFNMIRLAATAMTANAPNAGGERGVIINTASVAAFDGQIGQAAYAA
SKGGVVAMTLAIARDLSRDGIRVMTIAPGIFETPMLLGMPPEVQDALGKMVPFPPRLGKPAEYAQLARAIIENPMLNGET
IRLDGAIRMQPK

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=255, Percent_Identity=56.8627450980392, Blast_Score=267, Evalue=8e-72,
Organism=Homo sapiens, GI83715985, Length=255, Percent_Identity=54.1176470588235, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI15277342, Length=259, Percent_Identity=32.4324324324324, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI40254992, Length=248, Percent_Identity=29.4354838709677, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI59889578, Length=207, Percent_Identity=33.8164251207729, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI31542939, Length=261, Percent_Identity=28.735632183908, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI50726952, Length=222, Percent_Identity=29.7297297297297, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787905, Length=230, Percent_Identity=33.9130434782609, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=29.5275590551181, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787335, Length=255, Percent_Identity=27.4509803921569, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI2367175, Length=229, Percent_Identity=33.6244541484716, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082160, Length=221, Percent_Identity=28.9592760180996, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789057, Length=213, Percent_Identity=29.5774647887324, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789208, Length=249, Percent_Identity=30.1204819277108, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=246, Percent_Identity=51.219512195122, Blast_Score=238, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI25147288, Length=256, Percent_Identity=31.640625, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=33.4645669291339, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560676, Length=259, Percent_Identity=25.0965250965251, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=228, Percent_Identity=31.5789473684211, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320089, Length=203, Percent_Identity=26.6009852216749, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17737361, Length=254, Percent_Identity=58.2677165354331, Blast_Score=280, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24639444, Length=251, Percent_Identity=33.8645418326693, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357041, Length=242, Percent_Identity=32.2314049586777, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571526, Length=237, Percent_Identity=31.6455696202532, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24644339, Length=239, Percent_Identity=28.8702928870293, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI23397609, Length=237, Percent_Identity=27.0042194092827, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 25643; Mature: 25643

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRDQVFIVTGGASGLGAGTTRALAEAGGKVVIADLNEAAGAALAQEIGGRFVRCDVSS
CCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCEEEEEECCC
EADGQAAVQAATSLGALAGLVNCAGIAPASRTVGKAGPHPLDQFARVININLIGTFNMIR
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEHHHHHH
LAATAMTANAPNAGGERGVIINTASVAAFDGQIGQAAYAASKGGVVAMTLAIARDLSRDG
HHHHHHCCCCCCCCCCCCEEEEECEEEEECCCCCHHHHHCCCCCEEEHHHHHHHHCCCCC
IRVMTIAPGIFETPMLLGMPPEVQDALGKMVPFPPRLGKPAEYAQLARAIIENPMLNGET
EEEEEECCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCE
IRLDGAIRMQPK
EEECCEEEECCC
>Mature Secondary Structure
MEIRDQVFIVTGGASGLGAGTTRALAEAGGKVVIADLNEAAGAALAQEIGGRFVRCDVSS
CCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCEEEEEECCC
EADGQAAVQAATSLGALAGLVNCAGIAPASRTVGKAGPHPLDQFARVININLIGTFNMIR
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEEEEEHHHHHH
LAATAMTANAPNAGGERGVIINTASVAAFDGQIGQAAYAASKGGVVAMTLAIARDLSRDG
HHHHHHCCCCCCCCCCCCEEEEECEEEEECCCCCHHHHHCCCCCEEEHHHHHHHHCCCCC
IRVMTIAPGIFETPMLLGMPPEVQDALGKMVPFPPRLGKPAEYAQLARAIIENPMLNGET
EEEEEECCCHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCE
IRLDGAIRMQPK
EEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]