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The map label for this gene is ispH

Identifier: 17547161

GI number: 17547161

Start: 2648677

End: 2649651

Strand: Reverse

Name: ispH

Synonym: RSc2442

Alternate gene names: 17547161

Gene position: 2649651-2648677 (Counterclockwise)

Preceding gene: 17547162

Following gene: 17547160

Centisome position: 71.3

GC content: 67.49

Gene sequence:

>975_bases
ATGAGCACGACCGAGAACGCTGCGATCGAACCCGTGACCGGCGCCGACGCCGAAGTCCTGCTGGCGCAGCCGCGCGGCTT
CTGCGCCGGCGTGGACCGCGCCATCGAGATCGTCGAGCGCGCGCTGCAGCGGTTCGGTGCGCCCATCTACGTGCGCCACG
AGATCGTGCACAACGCCTACGTGGTGAGCGATCTGCGCAGCAAGGGCGCGGTGTTCGTGCAGGAGCTGGACGACGTGCCG
GTGGGCGGTACGGTCATCTTCAGCGCGCACGGCGTGTCGCGCGCCGTTCGCCAGGCCGCCGAGGCGCGCGGCCTGCGCGT
GTTCGATGCGACCTGCCCGCTGGTGACCAAGGTGCATGTCGAAGTGTCGAAGATGCGCACCCAGGGCTTCGAGATCATCA
TGATTGGTCACAAGGGCCATCCGGAAGTCGAGGGCACCATGGGGCAGGCCGACGACGGCATGCTGCTGGTCGAGTCGGTG
GACGACGTGGCGCGCCTGGCCGTCAAGGATCCGGCGCGGCTGGCCTACGTTACGCAGACCACGCTGTCGGTGGACGAGAC
GCAGGAGATCGTCGCGGCGATCAAGGCGCGCTTTCCTGCCGTGCACGAGCCCAAGAAGCAGGACATCTGCTATGCCACGC
AGAACCGGCAGGACGCAGTCAAGTTCATGGCGCCGCAGGTCGAGGTCGTGATCGTGGTCGGCAGTCCGAACAGCTCGAAC
TCCAACCGGCTGCGCGAGCTGGCCGAAAAGCTGGGCGTGCCCGCCTATATGGTGGATACCCCGGAGCAGGTCAGGCCGGA
GTGGCTGGCGGGCAAGCGCCGCGTCGGCCTGACGGCGGGGGCGTCCGCGCCGGAGGAGCTGGCGCAGTCGATCGTCGATC
GCCTGCGCGCACTCGGTGCGCGCTCCGTGCGTCCGCTCGACGGCATCCAGGAAAACATGTCTTTCCCTCTTCCGAGGGGA
CTTCAAATCAATTGA

Upstream 100 bases:

>100_bases
TGCTGAAGGAGGTCGGTGAGACCGCGGCGCTGTTCGATTTCAATCATCCGCTGGCCGGGCAGACCATCCTGTTCGAAGTC
CAGCTGATCGGCATCCTGTG

Downstream 100 bases:

>100_bases
TTGGCATGATGTAAATGTCTGCTTAATTGATCGCACGGTAATGGTGCGCACAATTGCGGTGCGTAAAGCGCTTTTATCGA
TATAGCGGCCACTCTTTCAG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSTTENAAIEPVTGADAEVLLAQPRGFCAGVDRAIEIVERALQRFGAPIYVRHEIVHNAYVVSDLRSKGAVFVQELDDVP
VGGTVIFSAHGVSRAVRQAAEARGLRVFDATCPLVTKVHVEVSKMRTQGFEIIMIGHKGHPEVEGTMGQADDGMLLVESV
DDVARLAVKDPARLAYVTQTTLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPNSSN
SNRLRELAEKLGVPAYMVDTPEQVRPEWLAGKRRVGLTAGASAPEELAQSIVDRLRALGARSVRPLDGIQENMSFPLPRG
LQIN

Sequences:

>Translated_324_residues
MSTTENAAIEPVTGADAEVLLAQPRGFCAGVDRAIEIVERALQRFGAPIYVRHEIVHNAYVVSDLRSKGAVFVQELDDVP
VGGTVIFSAHGVSRAVRQAAEARGLRVFDATCPLVTKVHVEVSKMRTQGFEIIMIGHKGHPEVEGTMGQADDGMLLVESV
DDVARLAVKDPARLAYVTQTTLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPNSSN
SNRLRELAEKLGVPAYMVDTPEQVRPEWLAGKRRVGLTAGASAPEELAQSIVDRLRALGARSVRPLDGIQENMSFPLPRG
LQIN
>Mature_323_residues
STTENAAIEPVTGADAEVLLAQPRGFCAGVDRAIEIVERALQRFGAPIYVRHEIVHNAYVVSDLRSKGAVFVQELDDVPV
GGTVIFSAHGVSRAVRQAAEARGLRVFDATCPLVTKVHVEVSKMRTQGFEIIMIGHKGHPEVEGTMGQADDGMLLVESVD
DVARLAVKDPARLAYVTQTTLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPNSSNS
NRLRELAEKLGVPAYMVDTPEQVRPEWLAGKRRVGLTAGASAPEELAQSIVDRLRALGARSVRPLDGIQENMSFPLPRGL
QIN

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=310, Percent_Identity=57.0967741935484, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_RALSO (P58677)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_520563.1
- GeneID:   1221289
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc2442
- NMPDR:   fig|267608.1.peg.2442
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   RSOL267608:RSC2442-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 35146; Mature: 35014

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTENAAIEPVTGADAEVLLAQPRGFCAGVDRAIEIVERALQRFGAPIYVRHEIVHNAY
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
VVSDLRSKGAVFVQELDDVPVGGTVIFSAHGVSRAVRQAAEARGLRVFDATCPLVTKVHV
HHHHHHCCCCEEEEHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEHH
EVSKMRTQGFEIIMIGHKGHPEVEGTMGQADDGMLLVESVDDVARLAVKDPARLAYVTQT
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCEEEEEEE
TLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPNSSN
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHCCCCEEEEEEEECCCCCC
SNRLRELAEKLGVPAYMVDTPEQVRPEWLAGKRRVGLTAGASAPEELAQSIVDRLRALGA
HHHHHHHHHHHCCCEEEECCHHHHCHHHHCCCCEECEECCCCCHHHHHHHHHHHHHHCCC
RSVRPLDGIQENMSFPLPRGLQIN
CCCCCHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STTENAAIEPVTGADAEVLLAQPRGFCAGVDRAIEIVERALQRFGAPIYVRHEIVHNAY
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
VVSDLRSKGAVFVQELDDVPVGGTVIFSAHGVSRAVRQAAEARGLRVFDATCPLVTKVHV
HHHHHHCCCCEEEEHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEHH
EVSKMRTQGFEIIMIGHKGHPEVEGTMGQADDGMLLVESVDDVARLAVKDPARLAYVTQT
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCEEEEEEE
TLSVDETQEIVAAIKARFPAVHEPKKQDICYATQNRQDAVKFMAPQVEVVIVVGSPNSSN
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHCCCCEEEEEEEECCCCCC
SNRLRELAEKLGVPAYMVDTPEQVRPEWLAGKRRVGLTAGASAPEELAQSIVDRLRALGA
HHHHHHHHHHHCCCEEEECCHHHHCHHHHCCCCEECEECCCCCHHHHHHHHHHHHHHCCC
RSVRPLDGIQENMSFPLPRGLQIN
CCCCCHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852