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The map label for this gene is cysB2 [H]

Identifier: 17547146

GI number: 17547146

Start: 2631674

End: 2632615

Strand: Direct

Name: cysB2 [H]

Synonym: RSc2427

Alternate gene names: 17547146

Gene position: 2631674-2632615 (Clockwise)

Preceding gene: 17547137

Following gene: 17547147

Centisome position: 70.81

GC content: 65.07

Gene sequence:

>942_bases
ATGAACCTGCACCAATTTCGCTTCGTGCGTGAAGCCGTGCGGCAGAAATTCAATCTGACCGAGGCGGCGAAAGCGCTCTA
TACATCACAACCCGGTGTCTCCAAGGCCATCATCGAGCTGGAGGAAGAACTGGGTGTCGATATCTTCACCCGGCATGGCA
AGCGCATCCGGGGGCTCACCGAGCCCGGGCGGCGCATCCTGGCATCGGTCGAGCGCGTGTTGCAGGAGGTGGACACGCTC
AAGCGCGTCGGCAAAGACTACGCCGCGCAGGACCAGGGCAATTTCACCATCGCCACCACGCACACGCAGGCGCGCTATGC
ACTGCCCAAGGCGATCGCCGAATTCACGCGCCGGTATCCGAAGGTGCGCCTGTCGATCCAGCAGGGCACGCCCGCGCAGA
TCGCCGAGATGGTCCTGCACGACCGCGCCGACATCGCCATCGCCACAGAAGGGCTGTCGCAGATCAAGGACCTGATCTCC
ATCCCCGGCTACCAGTGGCAGCACGTGGTGGTGACGCCGGCCGACCACCCGCTGCTGTCGCGCCAGCACCTGACGCTGGA
AGACCTGAAGAACTATCCGATCATCACCTACGACCAGCACTTCGCCGGGCGCCCCAAGATCGACCACGCCTTCGAGCTGC
GCCAGCTCAAGCCCGACATCGTGCTGGAGGCCATCGACGCGGACGTCATCAAGACCTACGTGGAGGTCGGGCTGGGCATC
GGCATCGTGGCCGGCGTGGCGTTCGACCCGGAGCGCGACCGCAACCTGCGCGCGATCCCGGCCGGCCACCTGTTCGGCAC
CAACGTCACCCACCTGGGGATCAAGCAGGGGGCCTACCTGCGCGGCTTCGTCTACACCTTCATCGAACTGTTCGCGCCGG
CGCTCACGCGCAAGCTGCTCGAGCAGCTGCTGGCGGGCGAGCACGAAGCGTACGAACTGTGA

Upstream 100 bases:

>100_bases
GGGCTCATATCCCAAACCAATAAAAAATTGTTTGTTTATATGGCTTCGGTTATAAGTAGCGGCTAGATCGCTCGTGCCAG
TTCCTTATTGACCGCGCATT

Downstream 100 bases:

>100_bases
GCGTGCCGCCCGCACCGGTTTCCCACCCGCTCCCGGAGACCCCATGACGACCCGACGACGCCTGCTGCACGGCCTGGCGC
TACTGGCCAGCGCCGCCGCC

Product: CysB family transcriptional regulator

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNLHQFRFVREAVRQKFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRGLTEPGRRILASVERVLQEVDTL
KRVGKDYAAQDQGNFTIATTHTQARYALPKAIAEFTRRYPKVRLSIQQGTPAQIAEMVLHDRADIAIATEGLSQIKDLIS
IPGYQWQHVVVTPADHPLLSRQHLTLEDLKNYPIITYDQHFAGRPKIDHAFELRQLKPDIVLEAIDADVIKTYVEVGLGI
GIVAGVAFDPERDRNLRAIPAGHLFGTNVTHLGIKQGAYLRGFVYTFIELFAPALTRKLLEQLLAGEHEAYEL

Sequences:

>Translated_313_residues
MNLHQFRFVREAVRQKFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRGLTEPGRRILASVERVLQEVDTL
KRVGKDYAAQDQGNFTIATTHTQARYALPKAIAEFTRRYPKVRLSIQQGTPAQIAEMVLHDRADIAIATEGLSQIKDLIS
IPGYQWQHVVVTPADHPLLSRQHLTLEDLKNYPIITYDQHFAGRPKIDHAFELRQLKPDIVLEAIDADVIKTYVEVGLGI
GIVAGVAFDPERDRNLRAIPAGHLFGTNVTHLGIKQGAYLRGFVYTFIELFAPALTRKLLEQLLAGEHEAYEL
>Mature_313_residues
MNLHQFRFVREAVRQKFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRGLTEPGRRILASVERVLQEVDTL
KRVGKDYAAQDQGNFTIATTHTQARYALPKAIAEFTRRYPKVRLSIQQGTPAQIAEMVLHDRADIAIATEGLSQIKDLIS
IPGYQWQHVVVTPADHPLLSRQHLTLEDLKNYPIITYDQHFAGRPKIDHAFELRQLKPDIVLEAIDADVIKTYVEVGLGI
GIVAGVAFDPERDRNLRAIPAGHLFGTNVTHLGIKQGAYLRGFVYTFIELFAPALTRKLLEQLLAGEHEAYEL

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=305, Percent_Identity=44.5901639344262, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788296, Length=296, Percent_Identity=46.9594594594595, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI157672245, Length=213, Percent_Identity=34.7417840375587, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI145693105, Length=253, Percent_Identity=29.2490118577075, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789204, Length=239, Percent_Identity=28.0334728033473, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788481, Length=248, Percent_Identity=30.241935483871, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788887, Length=248, Percent_Identity=26.6129032258064, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787806, Length=243, Percent_Identity=25.1028806584362, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790399, Length=259, Percent_Identity=23.9382239382239, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082132, Length=298, Percent_Identity=24.1610738255034, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI2367136, Length=272, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787879, Length=262, Percent_Identity=26.3358778625954, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081904, Length=273, Percent_Identity=26.7399267399267, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=240, Percent_Identity=24.5833333333333, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35231; Mature: 35231

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHQFRFVREAVRQKFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRGLT
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCHHCCCC
EPGRRILASVERVLQEVDTLKRVGKDYAAQDQGNFTIATTHTQARYALPKAIAEFTRRYP
CHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KVRLSIQQGTPAQIAEMVLHDRADIAIATEGLSQIKDLISIPGYQWQHVVVTPADHPLLS
EEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
RQHLTLEDLKNYPIITYDQHFAGRPKIDHAFELRQLKPDIVLEAIDADVIKTYVEVGLGI
CCCCCHHHHCCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCH
GIVAGVAFDPERDRNLRAIPAGHLFGTNVTHLGIKQGAYLRGFVYTFIELFAPALTRKLL
HHHHCCEECCCCCCCCEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
EQLLAGEHEAYEL
HHHHCCCCHHCCC
>Mature Secondary Structure
MNLHQFRFVREAVRQKFNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRGLT
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCHHCCCC
EPGRRILASVERVLQEVDTLKRVGKDYAAQDQGNFTIATTHTQARYALPKAIAEFTRRYP
CHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KVRLSIQQGTPAQIAEMVLHDRADIAIATEGLSQIKDLISIPGYQWQHVVVTPADHPLLS
EEEEEECCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCC
RQHLTLEDLKNYPIITYDQHFAGRPKIDHAFELRQLKPDIVLEAIDADVIKTYVEVGLGI
CCCCCHHHHCCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCH
GIVAGVAFDPERDRNLRAIPAGHLFGTNVTHLGIKQGAYLRGFVYTFIELFAPALTRKLL
HHHHCCEECCCCCCCCEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
EQLLAGEHEAYEL
HHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]