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The map label for this gene is hemD [H]

Identifier: 17547075

GI number: 17547075

Start: 2554880

End: 2556967

Strand: Reverse

Name: hemD [H]

Synonym: RSc2356

Alternate gene names: 17547075

Gene position: 2556967-2554880 (Counterclockwise)

Preceding gene: 17547076

Following gene: 17547074

Centisome position: 68.8

GC content: 73.99

Gene sequence:

>2088_bases
ATGGACGACCGCTCGCCTTCTCCATCCAGCGTGCCGCCCGACGCTCTCCCGCATCCTTCCGCGCCCGTCGTCATCGTCAC
GCGGCCGCGCGCGCAGGCGCCCATGCTGGTTGCCGCGCTGGAGCGCCACGGGCTGCGCACGCTGCAGTTTCCGCTGCTCG
ACATCGCGCCGACGCCCAACCTGGACGACCTGCGTGCTGCGCTGGCCGATCCGTCGCGCTATGCGCTGGTGGTGTTCGTC
AGCCCCAATGCCGTGCAGCAGGCGTTTGCGGCCATGCCCGAAGGCTTCCGCTGGCCGCAGGATGTGCCGGCCGCGGTGGT
CGGCCCCGCCAGCGCCCAGGCCCTGGCCGCGCACGGCGTCGCGGCGCCCACGTACCGCGTGATCCGGCCCGACTCCCATG
CCGACGACGCGCGGCAGGATTCCGAGGCGCTGTATGCGCGCCTGGACGTGGCCACGCTGAGCGGCCGCGAAGTGCTGATC
GTGCGCGGCAACGGCGGCCGCGAATGGCTGGCCGACCGGCTGCGCGAAGCGGGCGTTGCCGTGCGCACGGTGGAGGCGTA
TCGGCGCAGTGTCCCGGTGCCCGACACCGCGGCCTGGGCCGCGCTGCGCGAGGTGCTGGCCGGCCGCCACGCCTGGACGC
TGACCAGCTCCGAGGCGGCGCGCAATCTCGACAGCCTTGCGCGCGCCAGCCTGAGCGCCGCCGAGGTCGAGGCGCTGTAC
GCGGCGCCCTGCTTCGCGCCCCATGCCCGCATTGTCGAGCAGGCACAGTCGCTGGGCTTTCGCGACGTGACGCTGACCGG
CGCCGGCGACGACCGCCTGCTGGCCGGCGTGCTCGCGTGGGCCGGCCCCGTCGCGCTCGCCGCCGAGACCACGCCGTCGC
CCGCCCCCGTGGCGGCGCCGGCCGAATCGAACCCTTCCTCACCTGCTCCGACCGTGTCGCAGACTCCCGTTCCCACGTCC
GTTCCCACACCGGGCCCGGCCGCCGCCTCGCCGGGGCTGGCTCCCGCGCCTGCCGCTCCGACCATGACCGCCGGCGCCGG
ACCCGGCCGCGGCGGCTGGCTGCTCTGGGCCGCGCTGATCCTGGCGCTCGTGGTGGTGGCCGGCCTGCAGATCCGCAATG
AGCGGCTGGCGCGCGAGGTCCGCCAGCGCGCGCAGCAGAACGAGACGCTGGCGCAGGAGATGCGCGTGCTGTCGCGCAAC
AATCAGGACGCCTTTGCCCAGCTGCAGCAGAAATTCGGCGCGCTGGACGCCCGCACCGCCGAAACGCGCGACCGCCAGGG
TGCGCTCGAGCAGGCCAGCCAGGACCTGCTGCGCAACCGCGACGATTGGCAGCGGGCCGAGATCGAGCGCTCGCTCGAAG
TCGCTTCCGAACAATTGCAGCTCACCGGCAACGTGAGCGGCGCGCTGGCCACACTGCAGACCATCGACGCTCGCCTGGCC
ACCGTCGACAAGCCGCAGTTCCGCGCCATGCGACGCGCTGTCGCTCGCGACATCGCCAAGCTCAAGGCCATGCCCGCCGT
CGACCTGTCGGGCGCCGCGATCCGGCTGGACGATGCGATCAACGGCATCGATGCGCTGCCGCTGGTGTCGTCCGCCGCGC
CGCTGGAGTCGGGGCAGGCCGCACCGGCCTCGCGGGGCCGTCCCGCCAAGCCGGGCAAGGGCGCGGCGCCGGCTTCGGCG
TTCGCGGCGTCGCCGGCGGCGGAGTCCGGCTGGTCGGCCGGCGTGCGCGCCTGGTGGAGCCGCCTGTGGCATGACGTGCG
CAGCGAGCTGGGCCAGATCGTGCAGGTACGCCGCGTCGACCAGACCGATGCGCTGCTGCTGTCGTCCGATCAGGCGTGGT
TCCTGCGCGAGAACCTCAAGCTGTGGCTGATGAACGCGCGGCTGGCGCTGCTGTCGCGCAACGAGGCCGTGTTCCGCGCC
GACCTCGGCCGCGCCGATACGCTGCTCGCGCGCTACTTCGACACGCAGTCGCCGCGCGTGGTGGCGGAGCAGACCCTGCT
GCAGCAGGCGCGCGCGAGCGTCGGCGCGCTGGAGGTGCCGACCCTGGGCGACAGTCTCGCCGCCGCCCGCGGCCAGGGCA
AGGAGTAA

Upstream 100 bases:

>100_bases
GCGCCCGCGTCGCGCAGCAGATGCTGGACGGCGGCGCACACGACATCCTCGCCACGCTGGGCGCCGACGCGCCGCCCGCC
ACCTGACCTCCCGGCCCGCC

Downstream 100 bases:

>100_bases
CCATGCGCTGGGTATTCTGGGTTGCGTTGCTGTTTGCCGCCGCCGTCGGCCTGGCGCTGTTCACCGAGCTCAACCTGAGC
AACGTCGTGCTGCTTTACCC

Product: bifunctional uroporphyrinogen-III synthetase/uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MDDRSPSPSSVPPDALPHPSAPVVIVTRPRAQAPMLVAALERHGLRTLQFPLLDIAPTPNLDDLRAALADPSRYALVVFV
SPNAVQQAFAAMPEGFRWPQDVPAAVVGPASAQALAAHGVAAPTYRVIRPDSHADDARQDSEALYARLDVATLSGREVLI
VRGNGGREWLADRLREAGVAVRTVEAYRRSVPVPDTAAWAALREVLAGRHAWTLTSSEAARNLDSLARASLSAAEVEALY
AAPCFAPHARIVEQAQSLGFRDVTLTGAGDDRLLAGVLAWAGPVALAAETTPSPAPVAAPAESNPSSPAPTVSQTPVPTS
VPTPGPAAASPGLAPAPAAPTMTAGAGPGRGGWLLWAALILALVVVAGLQIRNERLAREVRQRAQQNETLAQEMRVLSRN
NQDAFAQLQQKFGALDARTAETRDRQGALEQASQDLLRNRDDWQRAEIERSLEVASEQLQLTGNVSGALATLQTIDARLA
TVDKPQFRAMRRAVARDIAKLKAMPAVDLSGAAIRLDDAINGIDALPLVSSAAPLESGQAAPASRGRPAKPGKGAAPASA
FAASPAAESGWSAGVRAWWSRLWHDVRSELGQIVQVRRVDQTDALLLSSDQAWFLRENLKLWLMNARLALLSRNEAVFRA
DLGRADTLLARYFDTQSPRVVAEQTLLQQARASVGALEVPTLGDSLAAARGQGKE

Sequences:

>Translated_695_residues
MDDRSPSPSSVPPDALPHPSAPVVIVTRPRAQAPMLVAALERHGLRTLQFPLLDIAPTPNLDDLRAALADPSRYALVVFV
SPNAVQQAFAAMPEGFRWPQDVPAAVVGPASAQALAAHGVAAPTYRVIRPDSHADDARQDSEALYARLDVATLSGREVLI
VRGNGGREWLADRLREAGVAVRTVEAYRRSVPVPDTAAWAALREVLAGRHAWTLTSSEAARNLDSLARASLSAAEVEALY
AAPCFAPHARIVEQAQSLGFRDVTLTGAGDDRLLAGVLAWAGPVALAAETTPSPAPVAAPAESNPSSPAPTVSQTPVPTS
VPTPGPAAASPGLAPAPAAPTMTAGAGPGRGGWLLWAALILALVVVAGLQIRNERLAREVRQRAQQNETLAQEMRVLSRN
NQDAFAQLQQKFGALDARTAETRDRQGALEQASQDLLRNRDDWQRAEIERSLEVASEQLQLTGNVSGALATLQTIDARLA
TVDKPQFRAMRRAVARDIAKLKAMPAVDLSGAAIRLDDAINGIDALPLVSSAAPLESGQAAPASRGRPAKPGKGAAPASA
FAASPAAESGWSAGVRAWWSRLWHDVRSELGQIVQVRRVDQTDALLLSSDQAWFLRENLKLWLMNARLALLSRNEAVFRA
DLGRADTLLARYFDTQSPRVVAEQTLLQQARASVGALEVPTLGDSLAAARGQGKE
>Mature_695_residues
MDDRSPSPSSVPPDALPHPSAPVVIVTRPRAQAPMLVAALERHGLRTLQFPLLDIAPTPNLDDLRAALADPSRYALVVFV
SPNAVQQAFAAMPEGFRWPQDVPAAVVGPASAQALAAHGVAAPTYRVIRPDSHADDARQDSEALYARLDVATLSGREVLI
VRGNGGREWLADRLREAGVAVRTVEAYRRSVPVPDTAAWAALREVLAGRHAWTLTSSEAARNLDSLARASLSAAEVEALY
AAPCFAPHARIVEQAQSLGFRDVTLTGAGDDRLLAGVLAWAGPVALAAETTPSPAPVAAPAESNPSSPAPTVSQTPVPTS
VPTPGPAAASPGLAPAPAAPTMTAGAGPGRGGWLLWAALILALVVVAGLQIRNERLAREVRQRAQQNETLAQEMRVLSRN
NQDAFAQLQQKFGALDARTAETRDRQGALEQASQDLLRNRDDWQRAEIERSLEVASEQLQLTGNVSGALATLQTIDARLA
TVDKPQFRAMRRAVARDIAKLKAMPAVDLSGAAIRLDDAINGIDALPLVSSAAPLESGQAAPASRGRPAKPGKGAAPASA
FAASPAAESGWSAGVRAWWSRLWHDVRSELGQIVQVRRVDQTDALLLSSDQAWFLRENLKLWLMNARLALLSRNEAVFRA
DLGRADTLLARYFDTQSPRVVAEQTLLQQARASVGALEVPTLGDSLAAARGQGKE

Specific function: Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen-III synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790235, Length=282, Percent_Identity=24.822695035461, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790236, Length=253, Percent_Identity=28.4584980237154, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003754 [H]

Pfam domain/function: PF02602 HEM4 [H]

EC number: =4.2.1.75 [H]

Molecular weight: Translated: 73671; Mature: 73671

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRSPSPSSVPPDALPHPSAPVVIVTRPRAQAPMLVAALERHGLRTLQFPLLDIAPTPN
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEECCCEECCCCCC
LDDLRAALADPSRYALVVFVSPNAVQQAFAAMPEGFRWPQDVPAAVVGPASAQALAAHGV
HHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHCCC
AAPTYRVIRPDSHADDARQDSEALYARLDVATLSGREVLIVRGNGGREWLADRLREAGVA
CCCCEEEECCCCCCCHHHHHHHHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHCCCH
VRTVEAYRRSVPVPDTAAWAALREVLAGRHAWTLTSSEAARNLDSLARASLSAAEVEALY
HEEHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
AAPCFAPHARIVEQAQSLGFRDVTLTGAGDDRLLAGVLAWAGPVALAAETTPSPAPVAAP
HCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
AESNPSSPAPTVSQTPVPTSVPTPGPAAASPGLAPAPAAPTMTAGAGPGRGGWLLWAALI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LALVVVAGLQIRNERLAREVRQRAQQNETLAQEMRVLSRNNQDAFAQLQQKFGALDARTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHH
ETRDRQGALEQASQDLLRNRDDWQRAEIERSLEVASEQLQLTGNVSGALATLQTIDARLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TVDKPQFRAMRRAVARDIAKLKAMPAVDLSGAAIRLDDAINGIDALPLVSSAAPLESGQA
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHCCCCHHHHHHCCCCCCCCCCC
APASRGRPAKPGKGAAPASAFAASPAAESGWSAGVRAWWSRLWHDVRSELGQIVQVRRVD
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QTDALLLSSDQAWFLRENLKLWLMNARLALLSRNEAVFRADLGRADTLLARYFDTQSPRV
CCCEEEEECCCCHHHHCCCEEEEEEHHEEEHCCCCHHHHHHCCHHHHHHHHHHCCCCCHH
VAEQTLLQQARASVGALEVPTLGDSLAAARGQGKE
HHHHHHHHHHHHHCCCEECCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MDDRSPSPSSVPPDALPHPSAPVVIVTRPRAQAPMLVAALERHGLRTLQFPLLDIAPTPN
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEECCCEECCCCCC
LDDLRAALADPSRYALVVFVSPNAVQQAFAAMPEGFRWPQDVPAAVVGPASAQALAAHGV
HHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHCCC
AAPTYRVIRPDSHADDARQDSEALYARLDVATLSGREVLIVRGNGGREWLADRLREAGVA
CCCCEEEECCCCCCCHHHHHHHHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHCCCH
VRTVEAYRRSVPVPDTAAWAALREVLAGRHAWTLTSSEAARNLDSLARASLSAAEVEALY
HEEHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
AAPCFAPHARIVEQAQSLGFRDVTLTGAGDDRLLAGVLAWAGPVALAAETTPSPAPVAAP
HCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
AESNPSSPAPTVSQTPVPTSVPTPGPAAASPGLAPAPAAPTMTAGAGPGRGGWLLWAALI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LALVVVAGLQIRNERLAREVRQRAQQNETLAQEMRVLSRNNQDAFAQLQQKFGALDARTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHH
ETRDRQGALEQASQDLLRNRDDWQRAEIERSLEVASEQLQLTGNVSGALATLQTIDARLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TVDKPQFRAMRRAVARDIAKLKAMPAVDLSGAAIRLDDAINGIDALPLVSSAAPLESGQA
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHCCCCHHHHHHCCCCCCCCCCC
APASRGRPAKPGKGAAPASAFAASPAAESGWSAGVRAWWSRLWHDVRSELGQIVQVRRVD
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QTDALLLSSDQAWFLRENLKLWLMNARLALLSRNEAVFRADLGRADTLLARYFDTQSPRV
CCCEEEEECCCCHHHHCCCEEEEEEHHEEEHCCCCHHHHHHCCHHHHHHHHHHCCCCCHH
VAEQTLLQQARASVGALEVPTLGDSLAAARGQGKE
HHHHHHHHHHHHHCCCEECCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8159168; 10984043 [H]